Changes in intestinal mucosal barrier function in obese mice v1 Article Swipe

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Xiaozhi Gao , Man Guan , Qiuju Li , Ruizhen Jia , Jianjun Deng ·

YOU? · · 2024 · Open Access · · DOI: https://doi.org/10.17504/protocols.io.bp2l6dbw5vqe/v1

Introduction Few reports have described changes in other functions of the intestinal mucosal barrier in obese patients worldwide. Objectives To observe the changes in intestinal mucosal barrier function in obese mice. Methods Fifty healthy male C57BL/6J mice were randomly divided into a high-fat diet (HFD)-induced obese group and a control group (CON), with 25 mice in each group. Plasma diamine oxidase (DAO), D-lactate and endotoxin levels were detected via the enzyme-labelled colorimetric method. Characteristics of intestinal flora in faeces were detected via Illumina HiSeq 16S rDNA high-throughput sequencing technology. The morphological characteristics of the ileal mucosa were observed via optical microscopy. The data were analysed via bioinformatics and statistics. Results The plasma levels of DAO, D-lactate, andendotoxin levels in the HFD group were significantly higher than those in the CON group at week 10（P＜0.05）. The plasma DAO, D-lactate, and endotoxin levels in the HFD group were significantly greater than in the CON group (P<0.05). The CON group contained more intestinal microbiota phyla and genera than the HFD group; the differences between the two groups were significant（FDR≤0.05, P≤0.05). The ileal mucosa of the HFD group exhibited more epithelial shedding at the intestinal villus tip, and the intestinal glands were more abundant. Conclusion This study is expected to provide a basic experimental reference for further research on the mechanism, prevention, and treatment of obesity.

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Barrier function Function (biology) Chemistry Cell biology Biology

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Type: preprint
Language: en
Landing Page: https://doi.org/10.17504/protocols.io.bp2l6dbw5vqe/v1
PDF: https://www.protocols.io/view/changes-in-intestinal-mucosal-barrier-function-in-dsdc6a2w.pdf
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DOI: https://doi.org/10.17504/protocols.io.bp2l6dbw5vqe/v1

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Title: Changes in intestinal mucosal barrier function in obese mice v1

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Type: preprint

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Language: en

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Publication year: 2024

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Publication date: 2024-11-26

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Authors: Xiaozhi Gao, Man Guan, Qiuju Li, Ruizhen Jia, Jianjun Deng

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Landing page: https://doi.org/10.17504/protocols.io.bp2l6dbw5vqe/v1

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Concepts: Barrier function, Function (biology), Chemistry, Cell biology, Biology

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