Characterization of alkaline metalloprotease isolated from halophilic bacterium Bacillus cereus and its applications in various industrial processes Article Swipe
KAINAT SAEED
,
SANIA RIAZ
,
A. Adil
,
Ismat Nawaz
,
Syed Kamran‐ul‐Hassan Naqvi
,
Ayesha Baig
,
Muhammad Ali
,
Iftikhar Zeb
,
RAZA AHMED
,
Tatheer Alam Naqvi
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1590/0001-3765202320230014
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.1590/0001-3765202320230014
Microbial proteases are one of the most demanding enzymes for various industries with diverse applications in food, pharmaceutics, and textile industries to name the few. An extracellular alkaline metalloprotease was produced and purified from moderate halophilic bacterial strain, Bacillus cereus TS2, with some unique characteristics required for various industrial applications. The protease was produced in basal medium supplemented with casein and was partially purified by ion exchange chromatography followed by ammonium sulphate precipitation. The alkaline metalloprotease has molecular weight of 35 kDa with specific activity of 535.4 µM/min/mg. It can work at wide range of pH from 3 to 12, while showing optimum activity at pH 10. Similarly, the alkaline metalloprotease is stable till the temperature of 80 °C and works at wide range of temperature from 20 to 90 °C with optimum activity at 60 °C. The turnover rate increases in the presence of NaCl and Co+2 with k cat/KM of 1.42 × 103 and 1.27 × 103 s-1.M-1 respectively, while without NaCl and Co+2 it has a value of 7.58× 102. The alkaline metalloprotease was relatively resistant to thermal and solvent mediated denaturation. Applications revealed that the metalloprotease was efficient to remove hair from goat skin, remove blood stains and degrade milk, thus can be a potential candidate for leather, detergent, and food industry.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1590/0001-3765202320230014
- https://www.scielo.br/j/aabc/a/mjZtHspTwQCR7xV4VdtH6YB/?lang=en&format=pdf
- OA Status
- diamond
- Cited By
- 3
- References
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- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4387813432
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4387813432Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1590/0001-3765202320230014Digital Object Identifier
- Title
-
Characterization of alkaline metalloprotease isolated from halophilic bacterium Bacillus cereus and its applications in various industrial processesWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2023Year of publication
- Publication date
-
2023-01-01Full publication date if available
- Authors
-
KAINAT SAEED, SANIA RIAZ, A. Adil, Ismat Nawaz, Syed Kamran‐ul‐Hassan Naqvi, Ayesha Baig, Muhammad Ali, Iftikhar Zeb, RAZA AHMED, Tatheer Alam NaqviList of authors in order
- Landing page
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https://doi.org/10.1590/0001-3765202320230014Publisher landing page
- PDF URL
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https://www.scielo.br/j/aabc/a/mjZtHspTwQCR7xV4VdtH6YB/?lang=en&format=pdfDirect link to full text PDF
- Open access
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YesWhether a free full text is available
- OA status
-
diamondOpen access status per OpenAlex
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https://www.scielo.br/j/aabc/a/mjZtHspTwQCR7xV4VdtH6YB/?lang=en&format=pdfDirect OA link when available
- Concepts
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Bacillus cereus, Proteases, Chemistry, Bacillus subtilis, Casein, Halophile, Metalloproteinase, Biochemistry, Bacteria, Enzyme, Protease, Chromatography, Food science, Microbiology, Biology, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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3Total citation count in OpenAlex
- Citations by year (recent)
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2025: 3Per-year citation counts (last 5 years)
- References (count)
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46Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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