Charting the nanotopography of inner hair cell synapses using MINFLUX nanoscopy Article Swipe
Rohan Kapoor
,
Hyo-Jin Kim
,
Evelyn Garlick
,
M. A. C. de Lima
,
Torben Ruhwedel
,
Wiebke Moebius
,
Fred Wolf
,
Tobias Moser
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1101/2025.04.22.649963
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1101/2025.04.22.649963
For us to hear, the cochlea encodes sounds into neural signals at synapses of inner hair cells (IHCs) and the auditory nerve with remarkable fidelity. To achieve the high rates of temporally precise synaptic transmission over long periods of time, IHCs employ sophisticated ribbon-type active zones (AZ). In order for us to understand synaptic sound encoding, we need to decipher the underpinning molecular topography of these synapse which had remained challenging due to technological limitations. Here we applied 3-dimensional minimal flux optical nanoscopy to mouse IHC synapses to chart the position of key pre- and postsynaptic proteins with single digit nanometre resolution of imaging. We demonstrate that nanoclusters of channels and interacting proteins govern the topography of AZs and postsynaptic densities (PSDs). We count synaptic proteins, their nanoclusters and determine their spatial organization feeding into computational modelling of AZ function. In conclusion, this study reveals a nanocluster-based molecular AZ and PSD topography, likely serving as functional modules in synaptic sound encoding.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2025.04.22.649963
- https://www.biorxiv.org/content/biorxiv/early/2025/04/25/2025.04.22.649963.full.pdf
- OA Status
- green
- References
- 112
- Related Works
- 10
- OpenAlex ID
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All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W4409803416Canonical identifier for this work in OpenAlex
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https://doi.org/10.1101/2025.04.22.649963Digital Object Identifier
- Title
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Charting the nanotopography of inner hair cell synapses using MINFLUX nanoscopyWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
-
2025-04-25Full publication date if available
- Authors
-
Rohan Kapoor, Hyo-Jin Kim, Evelyn Garlick, M. A. C. de Lima, Torben Ruhwedel, Wiebke Moebius, Fred Wolf, Tobias MoserList of authors in order
- Landing page
-
https://doi.org/10.1101/2025.04.22.649963Publisher landing page
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https://www.biorxiv.org/content/biorxiv/early/2025/04/25/2025.04.22.649963.full.pdfDirect link to full text PDF
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YesWhether a free full text is available
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-
greenOpen access status per OpenAlex
- OA URL
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https://www.biorxiv.org/content/biorxiv/early/2025/04/25/2025.04.22.649963.full.pdfDirect OA link when available
- Concepts
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Nanotopography, Nanotechnology, Materials scienceTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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| abstract_inverted_index.sophisticated | 43 |
| abstract_inverted_index.technological | 74 |
| abstract_inverted_index.nanocluster-based | 147 |
| cited_by_percentile_year | |
| countries_distinct_count | 0 |
| institutions_distinct_count | 8 |
| citation_normalized_percentile.value | 0.16569453 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |