Click and Detect: Versatile Ampicillin Aptasensor Enabled by Click Chemistry on a Graphene-Alkyne Derivative Article Swipe
José M.R. Flauzino
,
Martin‐Alex Nalepa
,
Demetrios D. Chronopoulos
,
Veronika Šedajová
,
David Panáček
,
Petr Jakubec
,
Petra Kührová
,
Martin Pykal
,
Pavel Banáš
,
Aleš Panáček
,
Aristides Bakandritsos
,
Michal Otyepka
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.5281/zenodo.7509720
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.5281/zenodo.7509720
Tackling the current problem of antimicrobial resistance requires fast, inexpensive and effective methods for controlling and detecting antibiotics in diverse samples at the point of interest. Cost-effective, disposable, point-of-care electrochemical biosensors are a particularly attractive option. However, there is a need for conductive and versatile carbon-based materials and inks that enable effective bioconjugation under mild conditions for the development of robust, sensitive and selective devices. This work describes a simple and fast methodology to construct an aptasensor based on a novel graphene derivative equipped with alkyne groups prepared via fluorographene chemistry. Using click chemistry, an aptamer was immobilized and used as a successful platform for the selective determination of ampicillin in real samples in the presence of interfering molecules. The electrochemical aptasensor displayed a detection limit of 1.36 nM, high selectivity among other antibiotics, storage stability of 4 weeks and was effective in real samples. Additionally, structural and docking simulations of the aptamer shed light on the ampicillin binding mechanism. The versatility of this platform opens up wide possibilities for constructing a new class of aptasensor based on disposable screen-printed carbon electrodes usable in point-of-care devices.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://zenodo.org/record/7509720
- OA Status
- green
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4317856806
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4317856806Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.5281/zenodo.7509720Digital Object Identifier
- Title
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Click and Detect: Versatile Ampicillin Aptasensor Enabled by Click Chemistry on a Graphene-Alkyne DerivativeWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2023Year of publication
- Publication date
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2023-01-23Full publication date if available
- Authors
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José M.R. Flauzino, Martin‐Alex Nalepa, Demetrios D. Chronopoulos, Veronika Šedajová, David Panáček, Petr Jakubec, Petra Kührová, Martin Pykal, Pavel Banáš, Aleš Panáček, Aristides Bakandritsos, Michal OtyepkaList of authors in order
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https://zenodo.org/record/7509720Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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greenOpen access status per OpenAlex
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https://zenodo.org/record/7509720Direct OA link when available
- Concepts
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Click chemistry, Alkyne, Combinatorial chemistry, Derivative (finance), Graphene, Nanotechnology, Chemistry, Computer science, Materials science, Organic chemistry, Financial economics, Catalysis, EconomicsTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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10Other works algorithmically related by OpenAlex
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