Clinical Validation of Loop‐Mediated Isothermal Amplification Assays for the Rapid Detection of Neisseria gonorrhoeae and Chlamydia trachomatis Article Swipe
Yasaman Ahmadi
,
Yejiong Yu
,
Zhanfeng Cui
,
Wei E. Huang
,
Monique Andersson
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1111/1751-7915.70214
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1111/1751-7915.70214
Neisseria gonorrhoeae (GC) and Chlamydia trachomatis (CT) are the predominant causes of bacterial sexually transmitted diseases. While nucleic acid amplification testing (NAATs), primarily polymerase chain reaction (PCR), is regarded as the gold standard for identifying these two pathogens, it usually takes a prolonged turnaround time and requires sophisticated equipment with considerable expense. In this study, we developed novel loop‐mediated isothermal amplification (LAMP) assays for rapid detection (< 30 min) of GC and CT in clinical urine and swab specimens. We analysed 208 clinical samples with three different pre‐treatment techniques including heating inactivation, centrifugation, and DNA extraction. LAMP results were compared with clinical results from the FDA‐approved BD ProbeTec ET assay. After heating inactivation, LAMP detected merely 41% and 65% of BD‐identified GC‐ and CT‐positive samples, respectively. Introducing centrifugation as an affordable and rapid pre‐treatment step increased detection rates to 81% and 91% for GC and CT, respectively. DNA extraction further enhanced the detection rates to 96% and 95% for GC‐ and CT‐LAMP, respectively. All these LAMP assays exhibited clinical specificity of ≥ 98%, underscoring the specificity of the chosen target genes (the porA pesudogene for GC and the ftsK gene for CT). Discrepant samples were verified by real‐time PCR; results were consistent with our LAMP findings. The overall LAMP performance met the WHO criteria for sensitivity and specificity for GC/CT point‐of‐care testing.
Related Topics
Concepts
Chlamydia trachomatis
Neisseria gonorrhoeae
Loop-mediated isothermal amplification
Nucleic Acid Amplification Tests
DNA extraction
Nucleic acid
Biology
Polymerase chain reaction
Chlamydiales
Chlamydia
Neisseria
Microbiology
Virology
DNA
Molecular biology
Bacteria
Gene
Immunology
Genetics
Biochemistry
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1111/1751-7915.70214
- https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/1751-7915.70214
- OA Status
- gold
- Cited By
- 1
- References
- 53
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4413810978
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4413810978Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1111/1751-7915.70214Digital Object Identifier
- Title
-
Clinical Validation of Loop‐Mediated Isothermal Amplification Assays for the Rapid Detection of Neisseria gonorrhoeae and Chlamydia trachomatisWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2025Year of publication
- Publication date
-
2025-08-29Full publication date if available
- Authors
-
Yasaman Ahmadi, Yejiong Yu, Zhanfeng Cui, Wei E. Huang, Monique AnderssonList of authors in order
- Landing page
-
https://doi.org/10.1111/1751-7915.70214Publisher landing page
- PDF URL
-
https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/1751-7915.70214Direct link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/1751-7915.70214Direct OA link when available
- Concepts
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Chlamydia trachomatis, Neisseria gonorrhoeae, Loop-mediated isothermal amplification, Nucleic Acid Amplification Tests, DNA extraction, Nucleic acid, Biology, Polymerase chain reaction, Chlamydiales, Chlamydia, Neisseria, Microbiology, Virology, DNA, Molecular biology, Bacteria, Gene, Immunology, Genetics, BiochemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
1Total citation count in OpenAlex
- Citations by year (recent)
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2025: 1Per-year citation counts (last 5 years)
- References (count)
-
53Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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