CLSM-Guided Imaging for Quantifying Endodontic Disinfection Article Swipe
YOU?
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· 2024
· Open Access
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· DOI: https://doi.org/10.3390/antibiotics13010054
Elimination of microbes in the root canal system is crucial for achieving long-term success in endodontic treatment. Further efforts in study design and standardization are needed in order to improve the validity and comparability of in vitro results on endodontic disinfection procedures, in turn improving clinical outcomes. This study optimizes two models at all steps: tooth selection, pretreatment, inoculation method (by growth or centrifugation), and confocal laser scanning microscopy (CLSM)-guided imaging of LIVE/DEAD-stained specimens. Individual anatomical conditions lead to substantial differences in penetration depth. Sclerosis grading (SCG), a classification system introduced in this study, provides information about the sclerosis status of the dentine and is helpful for careful, specific, and comparable tooth selection in in vitro studies. Sonically activated EDTA for the pretreatment of roots, inoculation of Enterococcus faecalis in an overflow model, 3–4 weeks of incubation, as well as polishing of dentine slices before staining, led to advances in the visualization of bacterial penetration and irrigation depths. In contrast, NaOCl pretreatment negatively affected performance reproducibility and should be avoided in any pretreatment. Nonsclerotized teeth (SCG0) can be used for microbial semilunar-shaped inoculation by centrifugation as a “quick-and-dirty” model for initial orientation. In conclusion, CLSM-guided imaging for quantifying endodontic infection/disinfection is a very powerful method after the fine-tuning of materials and methods.
Related Topics
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/antibiotics13010054
- https://www.mdpi.com/2079-6382/13/1/54/pdf?version=1704378526
- OA Status
- gold
- Cited By
- 3
- References
- 39
- Related Works
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- OpenAlex ID
- https://openalex.org/W4390579493
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4390579493Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/antibiotics13010054Digital Object Identifier
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CLSM-Guided Imaging for Quantifying Endodontic DisinfectionWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-01-04Full publication date if available
- Authors
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R. Mattern, Sabrina Ernst, Sarah Böcher, Andreas Braun, Johannes-Simon Wenzler, Georg ConradsList of authors in order
- Landing page
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https://doi.org/10.3390/antibiotics13010054Publisher landing page
- PDF URL
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https://www.mdpi.com/2079-6382/13/1/54/pdf?version=1704378526Direct link to full text PDF
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://www.mdpi.com/2079-6382/13/1/54/pdf?version=1704378526Direct OA link when available
- Concepts
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Root canal, Confocal laser scanning microscopy, Biomedical engineering, Dentistry, Penetration (warfare), Dentin, Medicine, Operations research, EngineeringTop concepts (fields/topics) attached by OpenAlex
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3Total citation count in OpenAlex
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2025: 2, 2024: 1Per-year citation counts (last 5 years)
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39Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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