Co‐Cultivation With New Glucose‐Sparing Chlorella Algae Boosts Tissue Culture Efficiency by Reducing Cell Waste Article Swipe
Melanie Oey
,
Ute C. Marx
,
Horst Joachim Schirra
,
Ian L. Ross
,
Robert G. Parton
,
Ben Hankamer
,
Harriet P. Lo
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/biot.70067
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.1002/biot.70067
Mammalian cell culture technologies are crucial for recombinant protein production, organoid generation, medical applications, and the generation of in vitro cultivated meat. However, they are limited by high costs, lack of vascular O 2 ‐provision, and the resultant inhibition of 3D tissue formation. Effective media and nutrient usage, oxygenation, and waste management are key to improvement. Microalgae utilize organic or inorganic CO 2 to produce O 2 from light, which complements O 2 ‐consuming and CO 2 ‐respiring mammalian cells in culture. However, common microalgal cultivation conditions differ in temperature and salinity from mammalian cell cultivation environments, making co‐cultivation short‐lived and challenging. We screened several different microalgae species to identify Chlorella sp. BDH‐1 (BDH‐1), which has high growth rates in mammalian culture conditions, but unlike other Chlorella species, does not compete for glucose as an energy source. In co‐culture, BDH‐1 reduces cellular waste production by maintaining mammalian cells in oxidative phosphorylation, which stabilizes pH, tripling culture longevity, and optimizes nutrient usage, which increases growth performance up to 80%. It further allows the reduction of expensive and ethically challenging fetal bovine serum requirements. Collectively, mammalian cell/BDH‐1 co‐cultivation improves tissue culture health and reduces costs, paving the path for applications in the biotechnology and medical sectors.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1002/biot.70067
- https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/biot.70067
- OA Status
- hybrid
- Cited By
- 1
- References
- 44
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4412015762
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4412015762Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1002/biot.70067Digital Object Identifier
- Title
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Co‐Cultivation With New Glucose‐Sparing Chlorella Algae Boosts Tissue Culture Efficiency by Reducing Cell WasteWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2025Year of publication
- Publication date
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2025-07-01Full publication date if available
- Authors
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Melanie Oey, Ute C. Marx, Horst Joachim Schirra, Ian L. Ross, Robert G. Parton, Ben Hankamer, Harriet P. LoList of authors in order
- Landing page
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https://doi.org/10.1002/biot.70067Publisher landing page
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/biot.70067Direct link to full text PDF
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/biot.70067Direct OA link when available
- Concepts
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Cell culture, Chlorella, Biology, Biomass (ecology), Nutrient, Algae, Biotechnology, Cell growth, Tissue culture, Cell biology, Biochemistry, In vitro, Botany, Ecology, GeneticsTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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