Life Science Alliance • Vol 6 • No 12
Comparative analysis of bioinformatics tools to characterize SARS-CoV-2 subgenomic RNAs
September 2023 • Denise Lavezzari, Antonio Mori, Elena Pomari, Michela Deiana, Antonio Fadda, Luca Bertoli, Alessandro Sinigaglia, Silvia Riccetti, Luisa Barzon, Chia…
During the replication of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), positive-sense genomic RNA and subgenomic RNAs (sgRNAs) are synthesized by a discontinuous process of transcription characterized by a template switch, regulated by transcription-regulating sequences (TRS). Although poorly known about makeup and dynamics of sgRNAs population and function of its constituents, next-generation sequencing approaches with the help of bioinformatics tools have made a significant contribution to expan…