Comparative evaluation of various serological tests in the laboratory diagnosis of Brucellosis Article Swipe
Meena Dias
,
Edwin Dias
·
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.4103/2348-3334.153258
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.4103/2348-3334.153258
Introduction: Brucellosis is a zoonotic disease of public health importance having an impact on animal husbandry and dairy industry. Isolation of bacteria from blood cultures is very low, and diagnosis mainly depends on serology and molecular methods. The aim of this study is to know the impact of various serological tests on the diagnosis of brucellosis. Materials and Methods: In a prospective study, a total of 180 samples, 90 from pyrexia of unknown origin (PUO) and 90 from high-risk group comprising veterinarians and animal handlers were serologically tested by Rose Bengal plate test, standard tube agglutination (STA) test and enzyme-linked immunosorbent assay (ELISA) and results were analyzed. Results: Out of 90 PUO cases, 28 were positive for brucellosis and in high-risk group, out of 30 veterinarians three were positive and out of 60 animal handlers 14 were positive. Male preponderance was seen. Rose Bengal and STA tests were still efficient methods for brucellosis serodiagnosis. The ELISA was observed to be more efficient in both acute and chronic brucellosis. Conclusion: All the three methods used are efficient methods for detecting Brucella antibody. Rose Bengal card test is the best suited for rapid diagnosis in rural endemic area as STA test is laborious and time-consuming. ELISA is the most sensitive test.
Related Topics
Concepts
Brucellosis
Medicine
Serology
Direct agglutination test
Brucella
Veterinary medicine
Rose bengal
Isolation (microbiology)
Antibody
Immunology
Microbiology
Biology
Genetics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.4103/2348-3334.153258
- OA Status
- diamond
- Cited By
- 5
- References
- 19
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W1992344875
All OpenAlex metadata
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https://openalex.org/W1992344875Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.4103/2348-3334.153258Digital Object Identifier
- Title
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Comparative evaluation of various serological tests in the laboratory diagnosis of BrucellosisWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2015Year of publication
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2015-01-01Full publication date if available
- Authors
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Meena Dias, Edwin DiasList of authors in order
- Landing page
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https://doi.org/10.4103/2348-3334.153258Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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diamondOpen access status per OpenAlex
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https://doi.org/10.4103/2348-3334.153258Direct OA link when available
- Concepts
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Brucellosis, Medicine, Serology, Direct agglutination test, Brucella, Veterinary medicine, Rose bengal, Isolation (microbiology), Antibody, Immunology, Microbiology, Biology, GeneticsTop concepts (fields/topics) attached by OpenAlex
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5Total citation count in OpenAlex
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2022: 1, 2020: 2, 2017: 1, 2016: 1Per-year citation counts (last 5 years)
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19Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.blood | 23 |
| abstract_inverted_index.dairy | 17 |
| abstract_inverted_index.group | 79 |
| abstract_inverted_index.plate | 91 |
| abstract_inverted_index.rapid | 190 |
| abstract_inverted_index.rural | 193 |
| abstract_inverted_index.seen. | 141 |
| abstract_inverted_index.still | 148 |
| abstract_inverted_index.study | 41 |
| abstract_inverted_index.test, | 92 |
| abstract_inverted_index.test. | 208 |
| abstract_inverted_index.tests | 50, 146 |
| abstract_inverted_index.three | 126, 171 |
| abstract_inverted_index.total | 64 |
| abstract_inverted_index.Bengal | 90, 143, 182 |
| abstract_inverted_index.animal | 14, 83, 133 |
| abstract_inverted_index.cases, | 112 |
| abstract_inverted_index.group, | 121 |
| abstract_inverted_index.having | 10 |
| abstract_inverted_index.health | 8 |
| abstract_inverted_index.impact | 12, 46 |
| abstract_inverted_index.mainly | 30 |
| abstract_inverted_index.origin | 73 |
| abstract_inverted_index.public | 7 |
| abstract_inverted_index.study, | 62 |
| abstract_inverted_index.suited | 188 |
| abstract_inverted_index.tested | 87 |
| abstract_inverted_index.(ELISA) | 102 |
| abstract_inverted_index.chronic | 166 |
| abstract_inverted_index.depends | 31 |
| abstract_inverted_index.disease | 5 |
| abstract_inverted_index.endemic | 194 |
| abstract_inverted_index.methods | 150, 172, 176 |
| abstract_inverted_index.pyrexia | 70 |
| abstract_inverted_index.results | 104 |
| abstract_inverted_index.unknown | 72 |
| abstract_inverted_index.various | 48 |
| abstract_inverted_index.Brucella | 179 |
| abstract_inverted_index.Methods: | 58 |
| abstract_inverted_index.Results: | 107 |
| abstract_inverted_index.bacteria | 21 |
| abstract_inverted_index.cultures | 24 |
| abstract_inverted_index.handlers | 84, 134 |
| abstract_inverted_index.methods. | 36 |
| abstract_inverted_index.observed | 157 |
| abstract_inverted_index.positive | 115, 128 |
| abstract_inverted_index.samples, | 67 |
| abstract_inverted_index.serology | 33 |
| abstract_inverted_index.standard | 93 |
| abstract_inverted_index.zoonotic | 4 |
| abstract_inverted_index.Isolation | 19 |
| abstract_inverted_index.Materials | 56 |
| abstract_inverted_index.analyzed. | 106 |
| abstract_inverted_index.antibody. | 180 |
| abstract_inverted_index.detecting | 178 |
| abstract_inverted_index.diagnosis | 29, 53, 191 |
| abstract_inverted_index.efficient | 149, 161, 175 |
| abstract_inverted_index.high-risk | 78, 120 |
| abstract_inverted_index.husbandry | 15 |
| abstract_inverted_index.industry. | 18 |
| abstract_inverted_index.laborious | 200 |
| abstract_inverted_index.molecular | 35 |
| abstract_inverted_index.positive. | 137 |
| abstract_inverted_index.sensitive | 207 |
| abstract_inverted_index.comprising | 80 |
| abstract_inverted_index.importance | 9 |
| abstract_inverted_index.Brucellosis | 1 |
| abstract_inverted_index.Conclusion: | 168 |
| abstract_inverted_index.brucellosis | 117, 152 |
| abstract_inverted_index.prospective | 61 |
| abstract_inverted_index.serological | 49 |
| abstract_inverted_index.brucellosis. | 55, 167 |
| abstract_inverted_index.Introduction: | 0 |
| abstract_inverted_index.agglutination | 95 |
| abstract_inverted_index.enzyme-linked | 99 |
| abstract_inverted_index.immunosorbent | 100 |
| abstract_inverted_index.preponderance | 139 |
| abstract_inverted_index.serologically | 86 |
| abstract_inverted_index.veterinarians | 81, 125 |
| abstract_inverted_index.serodiagnosis. | 153 |
| abstract_inverted_index.time-consuming. | 202 |
| cited_by_percentile_year.max | 96 |
| cited_by_percentile_year.min | 89 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 2 |
| citation_normalized_percentile.value | 0.75965462 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |