Comparison of Different Decalcification Methods Using Rat Mandibles as a Model Article Swipe
Flávia Medeiros Savi
,
Gary Brierly
,
Jeremy Baldwin
,
Christina Theodoropoulos
,
Maria A. Woodruff
·
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.1369/0022155417733708
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.1369/0022155417733708
Selection of decalcification agents is an essential consideration when processing mineralized tissues because the integrity and immunohistochemical characteristics of the tissues may be affected. Here, we report results obtained from the decalcification of rat mandibles using 10% ethylenediaminetetraacetic acid (EDTA) at room temperature (RT), 10% EDTA at 37C, 5% nitric acid, and 10% formic acid at RT. Decalcification endpoints were determined by microcomputed tomography. Morphological preservation and antigenicity were evaluated by hematoxylin and eosin staining and immunohistochemistry. Decalcification of the anterior and posterior portions of the mandible took 220 and 191 hr in 10% EDTA RT, 102 and 73 hr in 10% EDTA 37C, 13.5 and 4.3 hr in 5% nitric acid, and 140 and 36 hr in 10% formic acid, respectively. Decalcification in 10% EDTA at 37C was accelerated, but 10% EDTA at RT provided optimal results for immunohistochemistry and cellular and structural details. Decalcification using 5% nitric acid was accomplished in the shortest time and exhibited good cellular and architectural morphology, whereas 10% formic acid was suboptimal with respect to tissue and cellular morphology. Despite being the slowest method, EDTA at RT is still the recommended method for decalcifying mineralized tissues; however, if rapid decalcification is needed, 5% nitric acid is the best option, yielding acceptable tissue integrity and speed.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1369/0022155417733708
- https://journals.sagepub.com/doi/pdf/10.1369/0022155417733708
- OA Status
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- Cited By
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- References
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- Related Works
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- OpenAlex ID
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All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2760007380Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1369/0022155417733708Digital Object Identifier
- Title
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Comparison of Different Decalcification Methods Using Rat Mandibles as a ModelWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2017Year of publication
- Publication date
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2017-09-29Full publication date if available
- Authors
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Flávia Medeiros Savi, Gary Brierly, Jeremy Baldwin, Christina Theodoropoulos, Maria A. WoodruffList of authors in order
- Landing page
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https://doi.org/10.1369/0022155417733708Publisher landing page
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https://journals.sagepub.com/doi/pdf/10.1369/0022155417733708Direct link to full text PDF
- Open access
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YesWhether a free full text is available
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bronzeOpen access status per OpenAlex
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https://journals.sagepub.com/doi/pdf/10.1369/0022155417733708Direct OA link when available
- Concepts
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Bone decalcification, Formic acid, Ethylenediaminetetraacetic acid, Nitric acid, Chemistry, Mineralized tissues, Staining, Immunohistochemistry, Biochemistry, Dentistry, Pathology, Chelation, Medicine, Dentin, Inorganic chemistry, Organic chemistryTop concepts (fields/topics) attached by OpenAlex
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91Total citation count in OpenAlex
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2025: 15, 2024: 11, 2023: 13, 2022: 11, 2021: 12Per-year citation counts (last 5 years)
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44Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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