Oncotarget • Vol 10 • No 55
Comparison of different semi-automated cfDNA extraction methods in combination with UMI-based targeted sequencing
October 2019 • Anna Streubel, Albrecht Stenzinger, Susann Stephan-Falkenau, Jens Kollmeier, Daniel Misch, Torsten Blum, Torsten T. Bauer, Olfert Landt, Alexander Am…
The analysis of circulating cell-free DNA (cfDNA) extracted from peripheral blood can serve as a minimally invasive alternative to tumor tissue biopsies in cases with impaired access to tissue. Its clinical utility has been well demonstrated for EGFR T790M testing in lung cancer patients suffering progress after tyrosine kinase inhibitor treatment. At present, highly sensitive unique molecular identifiers (UMI)-based NGS for liquid biopsy testing is less established compared to single gene assays. However, the cri…