Connecting structure to function with the recovery of over 1000 high-quality activated sludge metagenome-assembled genomes encoding full-length rRNA genes using long-read sequencing Article Swipe
Caitlin M. Singleton
,
Francesca Petriglieri
,
Jannie Munk Kristensen
,
Rasmus Hansen Kirkegaard
,
Thomas Yssing Michaelsen
,
Martin Hjorth Andersen
,
Zivile Kondrotaite
,
Søren Michael Karst
,
Morten Simonsen Dueholm
,
Per Halkjær Nielsen
,
Mads Albertsen
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1101/2020.05.12.088096
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1101/2020.05.12.088096
Microorganisms are critical to water recycling, pollution removal and resource recovery processes in the wastewater industry. While the structure of this complex community is increasingly understood based on 16S rRNA gene studies, this structure cannot currently be linked to functional potential due to the absence of high-quality metagenome-assembled genomes (MAGs) with full-length rRNA genes for nearly all species. Here, we sequence 23 Danish full-scale wastewater treatment plant metagenomes, producing >1 Tbp of long-read and >0.9 Tbp of short-read data. We recovered 1083 high-quality MAGs, including 57 closed circular genomes. The MAGs accounted for ~30% of the community, and meet the stringent MIMAG high-quality draft requirements including full-length rRNA genes. We show how novel high-quality MAGs in combination with >13 years of amplicon data, Raman microspectroscopy and fluorescence in situ hybridisation can be used to uncover abundant undescribed lineages belonging to important functional groups.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2020.05.12.088096
- https://www.biorxiv.org/content/biorxiv/early/2020/05/12/2020.05.12.088096.full.pdf
- OA Status
- green
- Cited By
- 29
- References
- 95
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3024501871
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3024501871Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1101/2020.05.12.088096Digital Object Identifier
- Title
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Connecting structure to function with the recovery of over 1000 high-quality activated sludge metagenome-assembled genomes encoding full-length rRNA genes using long-read sequencingWork title
- Type
-
preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2020Year of publication
- Publication date
-
2020-05-12Full publication date if available
- Authors
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Caitlin M. Singleton, Francesca Petriglieri, Jannie Munk Kristensen, Rasmus Hansen Kirkegaard, Thomas Yssing Michaelsen, Martin Hjorth Andersen, Zivile Kondrotaite, Søren Michael Karst, Morten Simonsen Dueholm, Per Halkjær Nielsen, Mads AlbertsenList of authors in order
- Landing page
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https://doi.org/10.1101/2020.05.12.088096Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2020/05/12/2020.05.12.088096.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
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https://www.biorxiv.org/content/biorxiv/early/2020/05/12/2020.05.12.088096.full.pdfDirect OA link when available
- Concepts
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Metagenomics, 16S ribosomal RNA, Biology, Amplicon, Genome, Ribosomal RNA, Gene, Computational biology, Genetics, Polymerase chain reactionTop concepts (fields/topics) attached by OpenAlex
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29Total citation count in OpenAlex
- Citations by year (recent)
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2025: 1, 2023: 3, 2022: 1, 2021: 14, 2020: 8Per-year citation counts (last 5 years)
- References (count)
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95Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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