CONSTRUCTION AND EXPRESSION OF QUARTET RECOMBINANT PEPTIDE SURFACTANT FOR EOR APPLICATION Article Swipe
Cut Nanda Sari
,
Usman Usman
,
Refiana Lestary
,
W R Riesa Khairunnisa
,
Leni Herlina
,
Syafrizal Syafrizal
,
Tati Kristianti
,
Sony Suhandono
·
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.29017/scog.50.3.98
YOU?
·
· 2016
· Open Access
·
· DOI: https://doi.org/10.29017/scog.50.3.98
The main drawback of the SUPEL peptide surfactant product which has been developed for EOR application is it isunstable at a high temperature. This research is aimed at generating the prototype of peptide surfactant construction in recombinant by stringing up 4 SUPEL linier sequences. Quartet recombinant technology can produce the peptide surfactant characterized as reversible biosurfactant, which is active at high temperature but inactive at low temperature. Multiple SUPEL Construction (MSC) that was developed in this research is using synthetic DNA and producing SUPEL in 4 sequences that can flip at normal temperature and can open when heated. SDS PAGE analysis results show that MSC construction can be expressed by inducting IPTG and cell harvested at 90°C. This research proves that construction and expression of the SUPEL quartet has been achieved by producing the peptide at an ideal size.
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Metadata
- Type
- article
- Language
- en
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- OA Status
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All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W3009902004Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.29017/scog.50.3.98Digital Object Identifier
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CONSTRUCTION AND EXPRESSION OF QUARTET RECOMBINANT PEPTIDE SURFACTANT FOR EOR APPLICATIONWork title
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articleOpenAlex work type
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enPrimary language
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2016Year of publication
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2016-01-01Full publication date if available
- Authors
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Cut Nanda Sari, Usman Usman, Refiana Lestary, W R Riesa Khairunnisa, Leni Herlina, Syafrizal Syafrizal, Tati Kristianti, Sony SuhandonoList of authors in order
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https://journal.lemigas.esdm.go.id/ojs/index.php/SCOG/article/view/98/0Publisher landing page
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greenOpen access status per OpenAlex
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https://journal.lemigas.esdm.go.id/ojs/index.php/SCOG/article/view/98/0Direct OA link when available
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Pulmonary surfactant, Recombinant DNA, Peptide, Chemistry, Biochemistry, GeneTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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1Other works algorithmically related by OpenAlex
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