Construction and Expression of Single Recombinant Peptide Surfactant for EOR Application Article Swipe
Cut Nanda Sari
,
Usman Usman
,
Riesa K. W Rohmat
,
Leni Herlina
,
KEN SAWITRI SULIANDRI
,
Onie Kristiawan
,
Dwiyantari Dwiyantari
,
Tati Kristianti
,
Sony Suhandono
·
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.5454/mi.11.1.5
YOU?
·
· 2017
· Open Access
·
· DOI: https://doi.org/10.5454/mi.11.1.5
Surfactant is generally synthetic chemical, which is effective and reliable. However, the chemicals usually did not degraded easily in the environment and could cause damage to the environment. The other possible alternative to produce surfactant is using genetic engineering in order to produce peptide based surfactant. In this research, peptide surfactant was produced using a gene construct which was created using overlapped polymerase chain reaction method (OE-PCR). PAGE analysis shows that single surfactant peptide construction can be expressed by induction of IPTG 1 mM and after at least twice sonication. This research proves that both two constructions have been successfully expressed by producing peptide in expected size (approximately 15 kDa).
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.5454/mi.11.1.5
- OA Status
- gold
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2768375521
All OpenAlex metadata
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https://openalex.org/W2768375521Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.5454/mi.11.1.5Digital Object Identifier
- Title
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Construction and Expression of Single Recombinant Peptide Surfactant for EOR ApplicationWork title
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articleOpenAlex work type
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enPrimary language
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2017Year of publication
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2017-01-01Full publication date if available
- Authors
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Cut Nanda Sari, Usman Usman, Riesa K. W Rohmat, Leni Herlina, KEN SAWITRI SULIANDRI, Onie Kristiawan, Dwiyantari Dwiyantari, Tati Kristianti, Sony SuhandonoList of authors in order
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https://doi.org/10.5454/mi.11.1.5Publisher landing page
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://doi.org/10.5454/mi.11.1.5Direct OA link when available
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Pulmonary surfactant, Peptide, Sonication, Recombinant DNA, Chemistry, Gene, Molecular biology, Chromatography, Biochemistry, Computational biology, BiologyTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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10Other works algorithmically related by OpenAlex
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