Retention time shifts across chromatographic fingerprints can negatively impact the reliability of quantitative and similarity analysis results. Hence, we present a novel two-dimensional convolution sharpening-based solution (Conv2dPA) for nonlinear peak alignment of co-eluting components in highly complex samples. The core of Conc2dPA is the exploitation of intrinsic bilinear structure in chromatographic-spectral data and the specially designed two-dimensional sharpening filters to compensate for the lack of chro…