Cost-Effective and High-Throughput LPS Detection via Microdroplet Technology in Biopharmaceuticals Article Swipe
Adriano Colombelli
,
Daniela Lospinoso
,
Valentina Arima
,
Vita Guarino
,
Alessandra Zizzari
,
Monica Bianco
,
Elisabetta Perrone
,
Luigi Carbone
,
R. Rella
,
Maria Grazia Manera
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.3390/bios15100649
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.3390/bios15100649
Lipopolysaccharides (LPS) from Gram-negative bacteria represent a significant challenge across various industries due to their prevalence and pathogenicity and the limitations of existing detection methods. Traditional approaches, such as the rabbit pyrogen test (RPT) and the Limulus Amebocyte Lysate (LAL) assay, have served as gold standards for endotoxin detection. However, these methods are constrained by high costs, lengthy processing times, environmental concerns, and the need for significant reagent volumes, which limit their scalability and application in resource-limited settings. In this study, we introduce an innovative microfluidic platform that integrates the LAL assay within microdroplets, addressing the critical limitations of traditional techniques. By leveraging the precise fluid control and reaction isolation offered by microdroplet technology, the system reduces reagent consumption, enhances sensitivity, and enables high-throughput analysis. Calibration tests were performed to validate the platform’s ability to detect LPS, using colorimetric measurements. Results demonstrated comparable or improved performance relative to traditional systems, achieving lower detection limits and greater accuracy. This work demonstrates a proof-of-concept miniaturisation of the pharmacopoeial LAL assay. The method yielded low intra-assay variability (σ ≈ 0.002 OD; CV ≈ 0.9% over n = 50 droplets per point) and a LOD estimated from calibration statistics after path-length normalisation. Broader adoption will require additional comparative validation and standardisation. This scalable, cost-effective, and environmentally sustainable approach offers a practical solution for endotoxin detection in clinical diagnostics, biopharmaceutical production, and environmental monitoring. The proposed technology paves the way for advanced LPS detection methods that meet stringent safety standards while improving efficiency, affordability, and adaptability for diverse applications.
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- Type
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- en
- Landing Page
- https://doi.org/10.3390/bios15100649
- https://www.mdpi.com/2079-6374/15/10/649/pdf?version=1759227223
- OA Status
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- 23
- OpenAlex ID
- https://openalex.org/W4414662462
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4414662462Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/bios15100649Digital Object Identifier
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Cost-Effective and High-Throughput LPS Detection via Microdroplet Technology in BiopharmaceuticalsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
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2025Year of publication
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2025-09-30Full publication date if available
- Authors
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Adriano Colombelli, Daniela Lospinoso, Valentina Arima, Vita Guarino, Alessandra Zizzari, Monica Bianco, Elisabetta Perrone, Luigi Carbone, R. Rella, Maria Grazia ManeraList of authors in order
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https://doi.org/10.3390/bios15100649Publisher landing page
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https://www.mdpi.com/2079-6374/15/10/649/pdf?version=1759227223Direct link to full text PDF
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://www.mdpi.com/2079-6374/15/10/649/pdf?version=1759227223Direct OA link when available
- Cited by
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0Total citation count in OpenAlex
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23Number of works referenced by this work
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