Critical points in Comet assay silver staining procedure Article Swipe

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M Milev , Viktorija Maksimova , Milkica Janeva , Tatjana Ruskovska ·

YOU? · · 2020 · Open Access · · DOI: https://doi.org/10.33320/maced.pharm.bull.2020.66.03.014

Comet assay is one of the most common methods for measuring DNA damage in eukaryotic cells. In 1984, Ostling and Johanson demonstrated movement of DNA strands when nuclei were exposed to an electric field. Later, Singh et al. (1998) modified and optimized the method using alkaline conditions during electrophoresis, thus increasing specificity and reproducibility of the method. In the recent years, this assay has gained in popularity because of its simple, economical, versatile and sensitive procedure. This assay requires only a small number of cells per sample and provides collection of data at individual cell level, allowing robust statistical analysis. According to Azqueta and Collins (2013), there is a number of comet assay variations, applicable to different types of samples, such as: peripheral blood cells, cell lines, buccal mucosa, yeasts, cancer cells and plant cells.

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Staining Comet Silver stain Chemistry Biology Molecular biology Pathology Medicine Astrobiology

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Type: article
Language: en
Landing Page: http://doi.org/10.33320/maced.pharm.bull.2020.66.03.014
PDF: https://doi.org/10.33320/maced.pharm.bull.2020.66.03.014
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DOI: https://doi.org/10.33320/maced.pharm.bull.2020.66.03.014

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Title: Critical points in Comet assay silver staining procedure

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Publication year: 2020

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Publication date: 2020-10-29

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Authors: M Milev, Viktorija Maksimova, Milkica Janeva, Tatjana Ruskovska

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Landing page: https://doi.org/10.33320/maced.pharm.bull.2020.66.03.014

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Concepts: Staining, Comet, Silver stain, Chemistry, Biology, Molecular biology, Pathology, Medicine, Astrobiology

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