Crystallization and X-ray analysis of the transcription-activator protein C1 of bacteriophage P22 in complex with the PREpromoter element Article Swipe
Avisek Mondal
,
Rajagopal Chattopadhyaya
,
Ajit B. Datta
,
Pradeep Parrack
·
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.1107/s2053230x15015708
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.1107/s2053230x15015708
The transcription-activator protein C1 of the temperate phage P22 of Salmonella typhimurium plays a key role in the lytic versus lysogenic switch of the phage. A homotetramer of 92-residue polypeptides, C1 binds to an approximate direct repeat similar to the transcription activator CII of coliphage λ. Despite this and several other similarities, including 57% sequence identity to coliphage CII, many biochemical observations on P22 C1 cannot be explained based on the structure of CII. To understand the molecular basis of these differences, C1 was overexpressed and purified and subjected to crystallization trials. Although no successful hits were obtained for the apoprotein, crystals could be obtained when the protein was subjected to crystallization trials in complex with a 23-mer promoter DNA fragment (P RE ). These crystals diffracted very well at the home source, allowing the collection of a 2.2 Å resolution data set. The C1–DNA crystals belonged to space group P 2 1 , with unit-cell parameters a = 87.27, b = 93.58, c = 111.16 Å, β = 94.51°. Solvent-content analysis suggests that the asymmetric unit contains three tetramer–DNA complexes. The three-dimensional structure is expected to shed light on the mechanism of activation by C1 and the molecular basis of its specificity.
Related Topics
Concepts
Bacteriophage
Transcription (linguistics)
Tetramer
DNA
Crystallization
Homotetramer
Biology
Promoter
Lytic cycle
Lysogenic cycle
Activator (genetics)
Molecular biology
Crystallography
Chemistry
Biophysics
Genetics
Gene
Escherichia coli
Biochemistry
Protein subunit
Gene expression
Virus
Organic chemistry
Linguistics
Philosophy
Enzyme
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1107/s2053230x15015708
- http://journals.iucr.org/f/issues/2015/10/00/wd5253/wd5253.pdf
- OA Status
- bronze
- Cited By
- 1
- References
- 29
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2178514860
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W2178514860Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1107/s2053230x15015708Digital Object Identifier
- Title
-
Crystallization and X-ray analysis of the transcription-activator protein C1 of bacteriophage P22 in complex with the PREpromoter elementWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2015Year of publication
- Publication date
-
2015-09-23Full publication date if available
- Authors
-
Avisek Mondal, Rajagopal Chattopadhyaya, Ajit B. Datta, Pradeep ParrackList of authors in order
- Landing page
-
https://doi.org/10.1107/s2053230x15015708Publisher landing page
- PDF URL
-
https://journals.iucr.org/f/issues/2015/10/00/wd5253/wd5253.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
bronzeOpen access status per OpenAlex
- OA URL
-
https://journals.iucr.org/f/issues/2015/10/00/wd5253/wd5253.pdfDirect OA link when available
- Concepts
-
Bacteriophage, Transcription (linguistics), Tetramer, DNA, Crystallization, Homotetramer, Biology, Promoter, Lytic cycle, Lysogenic cycle, Activator (genetics), Molecular biology, Crystallography, Chemistry, Biophysics, Genetics, Gene, Escherichia coli, Biochemistry, Protein subunit, Gene expression, Virus, Organic chemistry, Linguistics, Philosophy, EnzymeTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
1Total citation count in OpenAlex
- Citations by year (recent)
-
2022: 1Per-year citation counts (last 5 years)
- References (count)
-
29Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W2178514860 |
|---|---|
| doi | https://doi.org/10.1107/s2053230x15015708 |
| ids.doi | https://doi.org/10.1107/s2053230x15015708 |
| ids.mag | 2178514860 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/26457520 |
| ids.openalex | https://openalex.org/W2178514860 |
| fwci | 0.0 |
| mesh[0].qualifier_ui | |
| mesh[0].descriptor_ui | D000595 |
| mesh[0].is_major_topic | False |
| mesh[0].qualifier_name | |
| mesh[0].descriptor_name | Amino Acid Sequence |
| mesh[1].qualifier_ui | Q000737 |
| mesh[1].descriptor_ui | D017100 |
| mesh[1].is_major_topic | False |
| mesh[1].qualifier_name | chemistry |
| mesh[1].descriptor_name | Bacteriophage P22 |
| mesh[2].qualifier_ui | |
| mesh[2].descriptor_ui | D002850 |
| mesh[2].is_major_topic | False |
| mesh[2].qualifier_name | |
| mesh[2].descriptor_name | Chromatography, Gel |
| mesh[3].qualifier_ui | |
| mesh[3].descriptor_ui | D003460 |
| mesh[3].is_major_topic | False |
| mesh[3].qualifier_name | |
| mesh[3].descriptor_name | Crystallization |
| mesh[4].qualifier_ui | |
| mesh[4].descriptor_ui | D018360 |
| mesh[4].is_major_topic | False |
| mesh[4].qualifier_name | |
| mesh[4].descriptor_name | Crystallography, X-Ray |
| mesh[5].qualifier_ui | |
| mesh[5].descriptor_ui | D008969 |
| mesh[5].is_major_topic | False |
| mesh[5].qualifier_name | |
| mesh[5].descriptor_name | Molecular Sequence Data |
| mesh[6].qualifier_ui | |
| mesh[6].descriptor_ui | D011401 |
| mesh[6].is_major_topic | True |
| mesh[6].qualifier_name | |
| mesh[6].descriptor_name | Promoter Regions, Genetic |
| mesh[7].qualifier_ui | |
| mesh[7].descriptor_ui | D017386 |
| mesh[7].is_major_topic | False |
| mesh[7].qualifier_name | |
| mesh[7].descriptor_name | Sequence Homology, Amino Acid |
| mesh[8].qualifier_ui | Q000737 |
| mesh[8].descriptor_ui | D014764 |
| mesh[8].is_major_topic | False |
| mesh[8].qualifier_name | chemistry |
| mesh[8].descriptor_name | Viral Proteins |
| mesh[9].qualifier_ui | |
| mesh[9].descriptor_ui | D000595 |
| mesh[9].is_major_topic | False |
| mesh[9].qualifier_name | |
| mesh[9].descriptor_name | Amino Acid Sequence |
| mesh[10].qualifier_ui | Q000737 |
| mesh[10].descriptor_ui | D017100 |
| mesh[10].is_major_topic | False |
| mesh[10].qualifier_name | chemistry |
| mesh[10].descriptor_name | Bacteriophage P22 |
| mesh[11].qualifier_ui | |
| mesh[11].descriptor_ui | D002850 |
| mesh[11].is_major_topic | False |
| mesh[11].qualifier_name | |
| mesh[11].descriptor_name | Chromatography, Gel |
| mesh[12].qualifier_ui | |
| mesh[12].descriptor_ui | D003460 |
| mesh[12].is_major_topic | False |
| mesh[12].qualifier_name | |
| mesh[12].descriptor_name | Crystallization |
| mesh[13].qualifier_ui | |
| mesh[13].descriptor_ui | D018360 |
| mesh[13].is_major_topic | False |
| mesh[13].qualifier_name | |
| mesh[13].descriptor_name | Crystallography, X-Ray |
| mesh[14].qualifier_ui | |
| mesh[14].descriptor_ui | D008969 |
| mesh[14].is_major_topic | False |
| mesh[14].qualifier_name | |
| mesh[14].descriptor_name | Molecular Sequence Data |
| mesh[15].qualifier_ui | |
| mesh[15].descriptor_ui | D011401 |
| mesh[15].is_major_topic | True |
| mesh[15].qualifier_name | |
| mesh[15].descriptor_name | Promoter Regions, Genetic |
| mesh[16].qualifier_ui | |
| mesh[16].descriptor_ui | D017386 |
| mesh[16].is_major_topic | False |
| mesh[16].qualifier_name | |
| mesh[16].descriptor_name | Sequence Homology, Amino Acid |
| mesh[17].qualifier_ui | Q000737 |
| mesh[17].descriptor_ui | D014764 |
| mesh[17].is_major_topic | False |
| mesh[17].qualifier_name | chemistry |
| mesh[17].descriptor_name | Viral Proteins |
| mesh[18].qualifier_ui | |
| mesh[18].descriptor_ui | D000595 |
| mesh[18].is_major_topic | False |
| mesh[18].qualifier_name | |
| mesh[18].descriptor_name | Amino Acid Sequence |
| mesh[19].qualifier_ui | Q000737 |
| mesh[19].descriptor_ui | D017100 |
| mesh[19].is_major_topic | False |
| mesh[19].qualifier_name | chemistry |
| mesh[19].descriptor_name | Bacteriophage P22 |
| mesh[20].qualifier_ui | |
| mesh[20].descriptor_ui | D002850 |
| mesh[20].is_major_topic | False |
| mesh[20].qualifier_name | |
| mesh[20].descriptor_name | Chromatography, Gel |
| mesh[21].qualifier_ui | |
| mesh[21].descriptor_ui | D003460 |
| mesh[21].is_major_topic | False |
| mesh[21].qualifier_name | |
| mesh[21].descriptor_name | Crystallization |
| mesh[22].qualifier_ui | |
| mesh[22].descriptor_ui | D018360 |
| mesh[22].is_major_topic | False |
| mesh[22].qualifier_name | |
| mesh[22].descriptor_name | Crystallography, X-Ray |
| mesh[23].qualifier_ui | |
| mesh[23].descriptor_ui | D008969 |
| mesh[23].is_major_topic | False |
| mesh[23].qualifier_name | |
| mesh[23].descriptor_name | Molecular Sequence Data |
| mesh[24].qualifier_ui | |
| mesh[24].descriptor_ui | D011401 |
| mesh[24].is_major_topic | True |
| mesh[24].qualifier_name | |
| mesh[24].descriptor_name | Promoter Regions, Genetic |
| mesh[25].qualifier_ui | |
| mesh[25].descriptor_ui | D017386 |
| mesh[25].is_major_topic | False |
| mesh[25].qualifier_name | |
| mesh[25].descriptor_name | Sequence Homology, Amino Acid |
| mesh[26].qualifier_ui | Q000737 |
| mesh[26].descriptor_ui | D014764 |
| mesh[26].is_major_topic | False |
| mesh[26].qualifier_name | chemistry |
| mesh[26].descriptor_name | Viral Proteins |
| type | article |
| title | Crystallization and X-ray analysis of the transcription-activator protein C1 of bacteriophage P22 in complex with the PREpromoter element |
| biblio.issue | 10 |
| biblio.volume | 71 |
| biblio.last_page | 1291 |
| biblio.first_page | 1286 |
| topics[0].id | https://openalex.org/T11048 |
| topics[0].field.id | https://openalex.org/fields/23 |
| topics[0].field.display_name | Environmental Science |
| topics[0].score | 0.9998999834060669 |
| topics[0].domain.id | https://openalex.org/domains/3 |
| topics[0].domain.display_name | Physical Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/2303 |
| topics[0].subfield.display_name | Ecology |
| topics[0].display_name | Bacteriophages and microbial interactions |
| topics[1].id | https://openalex.org/T10120 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9994999766349792 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1311 |
| topics[1].subfield.display_name | Genetics |
| topics[1].display_name | Bacterial Genetics and Biotechnology |
| topics[2].id | https://openalex.org/T11162 |
| topics[2].field.id | https://openalex.org/fields/25 |
| topics[2].field.display_name | Materials Science |
| topics[2].score | 0.9987999796867371 |
| topics[2].domain.id | https://openalex.org/domains/3 |
| topics[2].domain.display_name | Physical Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2505 |
| topics[2].subfield.display_name | Materials Chemistry |
| topics[2].display_name | Enzyme Structure and Function |
| is_xpac | False |
| apc_list.value | 3100 |
| apc_list.currency | USD |
| apc_list.value_usd | 3100 |
| apc_paid | |
| concepts[0].id | https://openalex.org/C2776441376 |
| concepts[0].level | 4 |
| concepts[0].score | 0.672072172164917 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q165028 |
| concepts[0].display_name | Bacteriophage |
| concepts[1].id | https://openalex.org/C179926584 |
| concepts[1].level | 2 |
| concepts[1].score | 0.6713937520980835 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q207714 |
| concepts[1].display_name | Transcription (linguistics) |
| concepts[2].id | https://openalex.org/C2778886173 |
| concepts[2].level | 3 |
| concepts[2].score | 0.6317955255508423 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q53832 |
| concepts[2].display_name | Tetramer |
| concepts[3].id | https://openalex.org/C552990157 |
| concepts[3].level | 2 |
| concepts[3].score | 0.563271701335907 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q7430 |
| concepts[3].display_name | DNA |
| concepts[4].id | https://openalex.org/C203036418 |
| concepts[4].level | 2 |
| concepts[4].score | 0.5420327186584473 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q284256 |
| concepts[4].display_name | Crystallization |
| concepts[5].id | https://openalex.org/C2778481663 |
| concepts[5].level | 4 |
| concepts[5].score | 0.5303006172180176 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q3408232 |
| concepts[5].display_name | Homotetramer |
| concepts[6].id | https://openalex.org/C86803240 |
| concepts[6].level | 0 |
| concepts[6].score | 0.5266133546829224 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[6].display_name | Biology |
| concepts[7].id | https://openalex.org/C101762097 |
| concepts[7].level | 4 |
| concepts[7].score | 0.5077344179153442 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q224093 |
| concepts[7].display_name | Promoter |
| concepts[8].id | https://openalex.org/C182615771 |
| concepts[8].level | 3 |
| concepts[8].score | 0.505810022354126 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q1369386 |
| concepts[8].display_name | Lytic cycle |
| concepts[9].id | https://openalex.org/C136199206 |
| concepts[9].level | 5 |
| concepts[9].score | 0.4981687068939209 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q1148110 |
| concepts[9].display_name | Lysogenic cycle |
| concepts[10].id | https://openalex.org/C88045685 |
| concepts[10].level | 3 |
| concepts[10].score | 0.4908638596534729 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q174576 |
| concepts[10].display_name | Activator (genetics) |
| concepts[11].id | https://openalex.org/C153911025 |
| concepts[11].level | 1 |
| concepts[11].score | 0.48921632766723633 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q7202 |
| concepts[11].display_name | Molecular biology |
| concepts[12].id | https://openalex.org/C8010536 |
| concepts[12].level | 1 |
| concepts[12].score | 0.4504568874835968 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q160398 |
| concepts[12].display_name | Crystallography |
| concepts[13].id | https://openalex.org/C185592680 |
| concepts[13].level | 0 |
| concepts[13].score | 0.3695361316204071 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[13].display_name | Chemistry |
| concepts[14].id | https://openalex.org/C12554922 |
| concepts[14].level | 1 |
| concepts[14].score | 0.3259807229042053 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q7100 |
| concepts[14].display_name | Biophysics |
| concepts[15].id | https://openalex.org/C54355233 |
| concepts[15].level | 1 |
| concepts[15].score | 0.3081992268562317 |
| concepts[15].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[15].display_name | Genetics |
| concepts[16].id | https://openalex.org/C104317684 |
| concepts[16].level | 2 |
| concepts[16].score | 0.29071611166000366 |
| concepts[16].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[16].display_name | Gene |
| concepts[17].id | https://openalex.org/C547475151 |
| concepts[17].level | 3 |
| concepts[17].score | 0.2389790415763855 |
| concepts[17].wikidata | https://www.wikidata.org/wiki/Q25419 |
| concepts[17].display_name | Escherichia coli |
| concepts[18].id | https://openalex.org/C55493867 |
| concepts[18].level | 1 |
| concepts[18].score | 0.2170340120792389 |
| concepts[18].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[18].display_name | Biochemistry |
| concepts[19].id | https://openalex.org/C104292427 |
| concepts[19].level | 3 |
| concepts[19].score | 0.16604095697402954 |
| concepts[19].wikidata | https://www.wikidata.org/wiki/Q899781 |
| concepts[19].display_name | Protein subunit |
| concepts[20].id | https://openalex.org/C150194340 |
| concepts[20].level | 3 |
| concepts[20].score | 0.13654044270515442 |
| concepts[20].wikidata | https://www.wikidata.org/wiki/Q26972 |
| concepts[20].display_name | Gene expression |
| concepts[21].id | https://openalex.org/C2522874641 |
| concepts[21].level | 2 |
| concepts[21].score | 0.09694728255271912 |
| concepts[21].wikidata | https://www.wikidata.org/wiki/Q808 |
| concepts[21].display_name | Virus |
| concepts[22].id | https://openalex.org/C178790620 |
| concepts[22].level | 1 |
| concepts[22].score | 0.0 |
| concepts[22].wikidata | https://www.wikidata.org/wiki/Q11351 |
| concepts[22].display_name | Organic chemistry |
| concepts[23].id | https://openalex.org/C41895202 |
| concepts[23].level | 1 |
| concepts[23].score | 0.0 |
| concepts[23].wikidata | https://www.wikidata.org/wiki/Q8162 |
| concepts[23].display_name | Linguistics |
| concepts[24].id | https://openalex.org/C138885662 |
| concepts[24].level | 0 |
| concepts[24].score | 0.0 |
| concepts[24].wikidata | https://www.wikidata.org/wiki/Q5891 |
| concepts[24].display_name | Philosophy |
| concepts[25].id | https://openalex.org/C181199279 |
| concepts[25].level | 2 |
| concepts[25].score | 0.0 |
| concepts[25].wikidata | https://www.wikidata.org/wiki/Q8047 |
| concepts[25].display_name | Enzyme |
| keywords[0].id | https://openalex.org/keywords/bacteriophage |
| keywords[0].score | 0.672072172164917 |
| keywords[0].display_name | Bacteriophage |
| keywords[1].id | https://openalex.org/keywords/transcription |
| keywords[1].score | 0.6713937520980835 |
| keywords[1].display_name | Transcription (linguistics) |
| keywords[2].id | https://openalex.org/keywords/tetramer |
| keywords[2].score | 0.6317955255508423 |
| keywords[2].display_name | Tetramer |
| keywords[3].id | https://openalex.org/keywords/dna |
| keywords[3].score | 0.563271701335907 |
| keywords[3].display_name | DNA |
| keywords[4].id | https://openalex.org/keywords/crystallization |
| keywords[4].score | 0.5420327186584473 |
| keywords[4].display_name | Crystallization |
| keywords[5].id | https://openalex.org/keywords/homotetramer |
| keywords[5].score | 0.5303006172180176 |
| keywords[5].display_name | Homotetramer |
| keywords[6].id | https://openalex.org/keywords/biology |
| keywords[6].score | 0.5266133546829224 |
| keywords[6].display_name | Biology |
| keywords[7].id | https://openalex.org/keywords/promoter |
| keywords[7].score | 0.5077344179153442 |
| keywords[7].display_name | Promoter |
| keywords[8].id | https://openalex.org/keywords/lytic-cycle |
| keywords[8].score | 0.505810022354126 |
| keywords[8].display_name | Lytic cycle |
| keywords[9].id | https://openalex.org/keywords/lysogenic-cycle |
| keywords[9].score | 0.4981687068939209 |
| keywords[9].display_name | Lysogenic cycle |
| keywords[10].id | https://openalex.org/keywords/activator |
| keywords[10].score | 0.4908638596534729 |
| keywords[10].display_name | Activator (genetics) |
| keywords[11].id | https://openalex.org/keywords/molecular-biology |
| keywords[11].score | 0.48921632766723633 |
| keywords[11].display_name | Molecular biology |
| keywords[12].id | https://openalex.org/keywords/crystallography |
| keywords[12].score | 0.4504568874835968 |
| keywords[12].display_name | Crystallography |
| keywords[13].id | https://openalex.org/keywords/chemistry |
| keywords[13].score | 0.3695361316204071 |
| keywords[13].display_name | Chemistry |
| keywords[14].id | https://openalex.org/keywords/biophysics |
| keywords[14].score | 0.3259807229042053 |
| keywords[14].display_name | Biophysics |
| keywords[15].id | https://openalex.org/keywords/genetics |
| keywords[15].score | 0.3081992268562317 |
| keywords[15].display_name | Genetics |
| keywords[16].id | https://openalex.org/keywords/gene |
| keywords[16].score | 0.29071611166000366 |
| keywords[16].display_name | Gene |
| keywords[17].id | https://openalex.org/keywords/escherichia-coli |
| keywords[17].score | 0.2389790415763855 |
| keywords[17].display_name | Escherichia coli |
| keywords[18].id | https://openalex.org/keywords/biochemistry |
| keywords[18].score | 0.2170340120792389 |
| keywords[18].display_name | Biochemistry |
| keywords[19].id | https://openalex.org/keywords/protein-subunit |
| keywords[19].score | 0.16604095697402954 |
| keywords[19].display_name | Protein subunit |
| keywords[20].id | https://openalex.org/keywords/gene-expression |
| keywords[20].score | 0.13654044270515442 |
| keywords[20].display_name | Gene expression |
| keywords[21].id | https://openalex.org/keywords/virus |
| keywords[21].score | 0.09694728255271912 |
| keywords[21].display_name | Virus |
| language | en |
| locations[0].id | doi:10.1107/s2053230x15015708 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S4210171526 |
| locations[0].source.issn | 2053-230X |
| locations[0].source.type | journal |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | 2053-230X |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | Acta Crystallographica Section F Structural Biology Communications |
| locations[0].source.host_organization | https://openalex.org/P4310320595 |
| locations[0].source.host_organization_name | Wiley |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320595 |
| locations[0].license | |
| locations[0].pdf_url | http://journals.iucr.org/f/issues/2015/10/00/wd5253/wd5253.pdf |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Acta Crystallographica Section F Structural Biology Communications |
| locations[0].landing_page_url | https://doi.org/10.1107/s2053230x15015708 |
| locations[1].id | pmid:26457520 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Acta crystallographica. Section F, Structural biology communications |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/26457520 |
| locations[2].id | pmh:oai:pubmedcentral.nih.gov:4601593 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S2764455111 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | PubMed Central |
| locations[2].source.host_organization | https://openalex.org/I1299303238 |
| locations[2].source.host_organization_name | National Institutes of Health |
| locations[2].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[2].license | |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | |
| locations[2].landing_page_url | http://doi.org/10.1107/S2053230X15015708 |
| indexed_in | crossref, pubmed |
| authorships[0].author.id | https://openalex.org/A5085668290 |
| authorships[0].author.orcid | https://orcid.org/0000-0003-1443-219X |
| authorships[0].author.display_name | Avisek Mondal |
| authorships[0].countries | IN |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I152754861 |
| authorships[0].affiliations[0].raw_affiliation_string | Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VIIM, Kolkata 700 054, India |
| authorships[0].institutions[0].id | https://openalex.org/I152754861 |
| authorships[0].institutions[0].ror | https://ror.org/01a5mqy88 |
| authorships[0].institutions[0].type | facility |
| authorships[0].institutions[0].lineage | https://openalex.org/I152754861 |
| authorships[0].institutions[0].country_code | IN |
| authorships[0].institutions[0].display_name | Bose Institute |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Avisek Mondal |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VIIM, Kolkata 700 054, India |
| authorships[1].author.id | https://openalex.org/A5080200652 |
| authorships[1].author.orcid | |
| authorships[1].author.display_name | Rajagopal Chattopadhyaya |
| authorships[1].countries | IN |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I152754861 |
| authorships[1].affiliations[0].raw_affiliation_string | Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VIIM, Kolkata 700 054, India |
| authorships[1].institutions[0].id | https://openalex.org/I152754861 |
| authorships[1].institutions[0].ror | https://ror.org/01a5mqy88 |
| authorships[1].institutions[0].type | facility |
| authorships[1].institutions[0].lineage | https://openalex.org/I152754861 |
| authorships[1].institutions[0].country_code | IN |
| authorships[1].institutions[0].display_name | Bose Institute |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Rajagopal Chattopadhyaya |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VIIM, Kolkata 700 054, India |
| authorships[2].author.id | https://openalex.org/A5103216612 |
| authorships[2].author.orcid | https://orcid.org/0000-0001-9695-4228 |
| authorships[2].author.display_name | Ajit B. Datta |
| authorships[2].countries | IN |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I152754861 |
| authorships[2].affiliations[0].raw_affiliation_string | Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VIIM, Kolkata 700 054, India |
| authorships[2].institutions[0].id | https://openalex.org/I152754861 |
| authorships[2].institutions[0].ror | https://ror.org/01a5mqy88 |
| authorships[2].institutions[0].type | facility |
| authorships[2].institutions[0].lineage | https://openalex.org/I152754861 |
| authorships[2].institutions[0].country_code | IN |
| authorships[2].institutions[0].display_name | Bose Institute |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Ajit Bikram Datta |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VIIM, Kolkata 700 054, India |
| authorships[3].author.id | https://openalex.org/A5050596874 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-2044-3787 |
| authorships[3].author.display_name | Pradeep Parrack |
| authorships[3].countries | IN |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I152754861 |
| authorships[3].affiliations[0].raw_affiliation_string | Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VIIM, Kolkata 700 054, India |
| authorships[3].institutions[0].id | https://openalex.org/I152754861 |
| authorships[3].institutions[0].ror | https://ror.org/01a5mqy88 |
| authorships[3].institutions[0].type | facility |
| authorships[3].institutions[0].lineage | https://openalex.org/I152754861 |
| authorships[3].institutions[0].country_code | IN |
| authorships[3].institutions[0].display_name | Bose Institute |
| authorships[3].author_position | last |
| authorships[3].raw_author_name | Pradeep Parrack |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VIIM, Kolkata 700 054, India |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | http://journals.iucr.org/f/issues/2015/10/00/wd5253/wd5253.pdf |
| open_access.oa_status | bronze |
| open_access.any_repository_has_fulltext | False |
| created_date | 2016-06-24T00:00:00 |
| display_name | Crystallization and X-ray analysis of the transcription-activator protein C1 of bacteriophage P22 in complex with the PREpromoter element |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T11048 |
| primary_topic.field.id | https://openalex.org/fields/23 |
| primary_topic.field.display_name | Environmental Science |
| primary_topic.score | 0.9998999834060669 |
| primary_topic.domain.id | https://openalex.org/domains/3 |
| primary_topic.domain.display_name | Physical Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/2303 |
| primary_topic.subfield.display_name | Ecology |
| primary_topic.display_name | Bacteriophages and microbial interactions |
| related_works | https://openalex.org/W2098176221, https://openalex.org/W2168167613, https://openalex.org/W2108639545, https://openalex.org/W95610762, https://openalex.org/W3198927870, https://openalex.org/W2768112850, https://openalex.org/W1984628064, https://openalex.org/W2148468498, https://openalex.org/W4285248991, https://openalex.org/W4255734500 |
| cited_by_count | 1 |
| counts_by_year[0].year | 2022 |
| counts_by_year[0].cited_by_count | 1 |
| locations_count | 3 |
| best_oa_location.id | doi:10.1107/s2053230x15015708 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4210171526 |
| best_oa_location.source.issn | 2053-230X |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | 2053-230X |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | Acta Crystallographica Section F Structural Biology Communications |
| best_oa_location.source.host_organization | https://openalex.org/P4310320595 |
| best_oa_location.source.host_organization_name | Wiley |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310320595 |
| best_oa_location.license | |
| best_oa_location.pdf_url | http://journals.iucr.org/f/issues/2015/10/00/wd5253/wd5253.pdf |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Acta Crystallographica Section F Structural Biology Communications |
| best_oa_location.landing_page_url | https://doi.org/10.1107/s2053230x15015708 |
| primary_location.id | doi:10.1107/s2053230x15015708 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S4210171526 |
| primary_location.source.issn | 2053-230X |
| primary_location.source.type | journal |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | 2053-230X |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | Acta Crystallographica Section F Structural Biology Communications |
| primary_location.source.host_organization | https://openalex.org/P4310320595 |
| primary_location.source.host_organization_name | Wiley |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320595 |
| primary_location.license | |
| primary_location.pdf_url | http://journals.iucr.org/f/issues/2015/10/00/wd5253/wd5253.pdf |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Acta Crystallographica Section F Structural Biology Communications |
| primary_location.landing_page_url | https://doi.org/10.1107/s2053230x15015708 |
| publication_date | 2015-09-23 |
| publication_year | 2015 |
| referenced_works | https://openalex.org/W2090636516, https://openalex.org/W2006615896, https://openalex.org/W2044479234, https://openalex.org/W2171054167, https://openalex.org/W2089142468, https://openalex.org/W2076108054, https://openalex.org/W1993545526, https://openalex.org/W2174616248, https://openalex.org/W2039316847, https://openalex.org/W2027485866, https://openalex.org/W1577586137, https://openalex.org/W1551139644, https://openalex.org/W2048315663, https://openalex.org/W2113832037, https://openalex.org/W2060807817, https://openalex.org/W2046802115, https://openalex.org/W2097498120, https://openalex.org/W2014694459, https://openalex.org/W2159211495, https://openalex.org/W2116947993, https://openalex.org/W2118374275, https://openalex.org/W2157477167, https://openalex.org/W2106882534, https://openalex.org/W2146080841, https://openalex.org/W1837297060, https://openalex.org/W2108921801, https://openalex.org/W1977515630, https://openalex.org/W2007389575, https://openalex.org/W2184594086 |
| referenced_works_count | 29 |
| abstract_inverted_index., | 153 |
| abstract_inverted_index.1 | 152 |
| abstract_inverted_index.2 | 151 |
| abstract_inverted_index.= | 158, 161, 164, 168 |
| abstract_inverted_index.A | 25 |
| abstract_inverted_index.P | 150 |
| abstract_inverted_index.a | 13, 116, 137, 157 |
| abstract_inverted_index.b | 160 |
| abstract_inverted_index.c | 163 |
| abstract_inverted_index.(P | 121 |
| abstract_inverted_index.). | 123 |
| abstract_inverted_index.C1 | 3, 30, 64, 82, 195 |
| abstract_inverted_index.RE | 122 |
| abstract_inverted_index.To | 74 |
| abstract_inverted_index.an | 33 |
| abstract_inverted_index.at | 129 |
| abstract_inverted_index.be | 66, 103 |
| abstract_inverted_index.by | 194 |
| abstract_inverted_index.in | 16, 113 |
| abstract_inverted_index.is | 184 |
| abstract_inverted_index.no | 93 |
| abstract_inverted_index.of | 4, 9, 22, 27, 43, 72, 79, 136, 192, 200 |
| abstract_inverted_index.on | 62, 69, 189 |
| abstract_inverted_index.to | 32, 38, 56, 89, 110, 147, 186 |
| abstract_inverted_index.Å | 139 |
| abstract_inverted_index.β | 167 |
| abstract_inverted_index.2.2 | 138 |
| abstract_inverted_index.57% | 53 |
| abstract_inverted_index.CII | 42 |
| abstract_inverted_index.DNA | 119 |
| abstract_inverted_index.P22 | 8, 63 |
| abstract_inverted_index.The | 0, 143, 181 |
| abstract_inverted_index.and | 48, 85, 87, 196 |
| abstract_inverted_index.for | 98 |
| abstract_inverted_index.its | 201 |
| abstract_inverted_index.key | 14 |
| abstract_inverted_index.the | 5, 17, 23, 39, 70, 76, 99, 106, 130, 134, 174, 190, 197 |
| abstract_inverted_index.was | 83, 108 |
| abstract_inverted_index.Å, | 166 |
| abstract_inverted_index.λ. | 45 |
| abstract_inverted_index.CII, | 58 |
| abstract_inverted_index.CII. | 73 |
| abstract_inverted_index.data | 141 |
| abstract_inverted_index.hits | 95 |
| abstract_inverted_index.home | 131 |
| abstract_inverted_index.many | 59 |
| abstract_inverted_index.role | 15 |
| abstract_inverted_index.set. | 142 |
| abstract_inverted_index.shed | 187 |
| abstract_inverted_index.that | 173 |
| abstract_inverted_index.this | 47 |
| abstract_inverted_index.unit | 176 |
| abstract_inverted_index.very | 127 |
| abstract_inverted_index.well | 128 |
| abstract_inverted_index.were | 96 |
| abstract_inverted_index.when | 105 |
| abstract_inverted_index.with | 115, 154 |
| abstract_inverted_index.These | 124 |
| abstract_inverted_index.based | 68 |
| abstract_inverted_index.basis | 78, 199 |
| abstract_inverted_index.binds | 31 |
| abstract_inverted_index.could | 102 |
| abstract_inverted_index.group | 149 |
| abstract_inverted_index.light | 188 |
| abstract_inverted_index.lytic | 18 |
| abstract_inverted_index.other | 50 |
| abstract_inverted_index.phage | 7 |
| abstract_inverted_index.plays | 12 |
| abstract_inverted_index.space | 148 |
| abstract_inverted_index.these | 80 |
| abstract_inverted_index.three | 178 |
| abstract_inverted_index.111.16 | 165 |
| abstract_inverted_index.23-mer | 117 |
| abstract_inverted_index.87.27, | 159 |
| abstract_inverted_index.93.58, | 162 |
| abstract_inverted_index.cannot | 65 |
| abstract_inverted_index.direct | 35 |
| abstract_inverted_index.phage. | 24 |
| abstract_inverted_index.repeat | 36 |
| abstract_inverted_index.switch | 21 |
| abstract_inverted_index.trials | 112 |
| abstract_inverted_index.versus | 19 |
| abstract_inverted_index.Despite | 46 |
| abstract_inverted_index.complex | 114 |
| abstract_inverted_index.protein | 2, 107 |
| abstract_inverted_index.several | 49 |
| abstract_inverted_index.similar | 37 |
| abstract_inverted_index.source, | 132 |
| abstract_inverted_index.trials. | 91 |
| abstract_inverted_index.94.51°. | 169 |
| abstract_inverted_index.Although | 92 |
| abstract_inverted_index.C1–DNA | 144 |
| abstract_inverted_index.allowing | 133 |
| abstract_inverted_index.analysis | 171 |
| abstract_inverted_index.belonged | 146 |
| abstract_inverted_index.contains | 177 |
| abstract_inverted_index.crystals | 101, 125, 145 |
| abstract_inverted_index.expected | 185 |
| abstract_inverted_index.fragment | 120 |
| abstract_inverted_index.identity | 55 |
| abstract_inverted_index.obtained | 97, 104 |
| abstract_inverted_index.promoter | 118 |
| abstract_inverted_index.purified | 86 |
| abstract_inverted_index.sequence | 54 |
| abstract_inverted_index.suggests | 172 |
| abstract_inverted_index.activator | 41 |
| abstract_inverted_index.coliphage | 44, 57 |
| abstract_inverted_index.explained | 67 |
| abstract_inverted_index.including | 52 |
| abstract_inverted_index.lysogenic | 20 |
| abstract_inverted_index.mechanism | 191 |
| abstract_inverted_index.molecular | 77, 198 |
| abstract_inverted_index.structure | 71, 183 |
| abstract_inverted_index.subjected | 88, 109 |
| abstract_inverted_index.temperate | 6 |
| abstract_inverted_index.unit-cell | 155 |
| abstract_inverted_index.92-residue | 28 |
| abstract_inverted_index.Salmonella | 10 |
| abstract_inverted_index.activation | 193 |
| abstract_inverted_index.asymmetric | 175 |
| abstract_inverted_index.collection | 135 |
| abstract_inverted_index.complexes. | 180 |
| abstract_inverted_index.diffracted | 126 |
| abstract_inverted_index.parameters | 156 |
| abstract_inverted_index.resolution | 140 |
| abstract_inverted_index.successful | 94 |
| abstract_inverted_index.understand | 75 |
| abstract_inverted_index.apoprotein, | 100 |
| abstract_inverted_index.approximate | 34 |
| abstract_inverted_index.biochemical | 60 |
| abstract_inverted_index.typhimurium | 11 |
| abstract_inverted_index.differences, | 81 |
| abstract_inverted_index.homotetramer | 26 |
| abstract_inverted_index.observations | 61 |
| abstract_inverted_index.specificity. | 202 |
| abstract_inverted_index.overexpressed | 84 |
| abstract_inverted_index.polypeptides, | 29 |
| abstract_inverted_index.similarities, | 51 |
| abstract_inverted_index.transcription | 40 |
| abstract_inverted_index.tetramer–DNA | 179 |
| abstract_inverted_index.Solvent-content | 170 |
| abstract_inverted_index.crystallization | 90, 111 |
| abstract_inverted_index.three-dimensional | 182 |
| abstract_inverted_index.transcription-activator | 1 |
| cited_by_percentile_year.max | 94 |
| cited_by_percentile_year.min | 89 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 4 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/14 |
| sustainable_development_goals[0].score | 0.6399999856948853 |
| sustainable_development_goals[0].display_name | Life below water |
| citation_normalized_percentile.value | 0.0544115 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |