Cytokine-Induced Transcriptional Changes in Human Neutrophils Reveal Immune Regulatory Plasticity Article Swipe
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· 2025
· Open Access
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· DOI: https://doi.org/10.1093/discim/kyaf013
· OA: W4414371456
Introduction Neutrophils are innate immune cells that play a central role in the inflammatory response. They produce an array of destructive molecules and antimicrobial proteases that characterise the cells as front-line defenders, crucial to host defence. It is now appreciated that neutrophils produce and respond to a variety of inflammatory signals and are able to regulate both the innate and adaptive immune responses. However, the mechanisms by which neutrophils respond to different inflammatory signals to regulate their own function and the functions of other immune cells are incompletely defined. Methods In this study, we performed RNA sequencing of healthy human neutrophils exposed for 1h to a range of pro-inflammatory cytokines. GM-CSF and TNFα induced significant changes in 1651 and 693 genes respectively (adj. p<0.05) including activation of genes regulating apoptosis and encoding cytokines and chemokines that can drive the differentiation and activation of CD4 T cells. Results Stimulation of neutrophils with G-CSF, IFNα, IFNγ, IL-1β, or IL-8 resulted in expression of discrete gene sets and differential activation of signalling pathways including changes in cell adhesion and migration, immune receptor expression, apoptosis, and production of pro-inflammatory prostaglandins. Conclusion This work defines the differential gene expression patterns in neutrophils exposed to different regulatory cytokines. This is important in both increasing our understanding of the role of neutrophils in driving innate and adaptive immune responses and, importantly, for deconvoluting the neutrophil gene expression signatures observed in inflammatory diseases.
