Data from Comparative Biomarker Expression and RNA Integrity in Biospecimens Derived from Radical Retropubic and Robot-Assisted Laparoscopic Prostatectomies Article Swipe

View

Carmela Ricciardelli , Tina Bianco‐Miotto , Shalini Jindal , Thomas J. Dodd , Penelope Cohen , Villis R. Marshall , Peter Sutherland , Hemamali Samaratunga , James G. Kench , Ying Dong , Hong Wang , Judith A. Clements , Gail P. Risbridger , Robert L. Sutherland , Wayne D. Tilley , David J. Horsfall ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1158/1055-9965.c.6514812.v1

Background: Knowledge of preanalytic conditions that biospecimens are subjected to is critically important because novel surgical procedures, tissue sampling, handling, and storage might affect biomarker expression or invalidate tissue samples as analytes for some technologies.Methods: We investigated differences in RNA quality, gene expression by quantitative real-time PCR, and immunoreactive protein expression of selected prostate cancer biomarkers between tissues from retropubic radical prostatectomy (RRP) and robot-assisted laparoscopic prostatectomy (RALP). Sections of tissue microarray of 23 RALP and 22 RRP samples were stained with antibodies to androgen receptor (AR) and prostate-specific antigen (PSA) as intersite controls, and 14 other candidate biomarkers of research interest to three laboratories within the Australian Prostate Cancer BioResource tissue banking network. Quantitative real-time PCR was done for AR, PSA (KLK3), KLK2, KLK4, and HIF1A on RNA extracted from five RALP and five RRP frozen tissue cores.Results: No histologic differences were observed between RALP and RRP tissue. Biomarker staining grouped these samples into those with increased (PSA, CK8/18, CKHMW, KLK4), decreased (KLK2, KLK14), or no change in expression (AR, ghrelin, Ki67, PCNA, VEGF-C, PAR2, YB1, p63, versican, and chondroitin 0-sulfate) in RALP compared with RRP tissue. No difference in RNA quality or gene expression was detected between RALP and RRP tissue.Conclusions: Changes in biomarker expression between RALP and RRP tissue exist at the immunoreactive protein level, but the etiology is unclear.Impact: Future studies should account for changes in biomarker expression when using RALP tissues, and mixed cohorts of RALP and RRP tissue should be avoided. Cancer Epidemiol Biomarkers Prev; 19(7); 1755–65. ©2010 AACR.

Related Topics

Concepts

Prostate cancer Tissue microarray Biomarker Prostate Prostatectomy Chemistry Medicine Cancer Internal medicine Biochemistry

Metadata

Type: preprint
Language: en
Landing Page: https://doi.org/10.1158/1055-9965.c.6514812.v1
OA Status: gold
Related Works: 10
OpenAlex ID: https://openalex.org/W4361822718

All OpenAlex metadata

Raw OpenAlex JSON

OpenAlex ID: https://openalex.org/W4361822718

Canonical identifier for this work in OpenAlex
DOI: https://doi.org/10.1158/1055-9965.c.6514812.v1

Digital Object Identifier
Title: Data from Comparative Biomarker Expression and RNA Integrity in Biospecimens Derived from Radical Retropubic and Robot-Assisted Laparoscopic Prostatectomies

Work title
Type: preprint

OpenAlex work type
Language: en

Primary language
Publication year: 2023

Year of publication
Publication date: 2023-03-31

Full publication date if available
Authors: Carmela Ricciardelli, Tina Bianco‐Miotto, Shalini Jindal, Thomas J. Dodd, Penelope Cohen, Villis R. Marshall, Peter Sutherland, Hemamali Samaratunga, James G. Kench, Ying Dong, Hong Wang, Judith A. Clements, Gail P. Risbridger, Robert L. Sutherland, Wayne D. Tilley, David J. Horsfall

List of authors in order
Landing page: https://doi.org/10.1158/1055-9965.c.6514812.v1

Publisher landing page
Open access: Yes

Whether a free full text is available
OA status: gold

Open access status per OpenAlex
OA URL: https://doi.org/10.1158/1055-9965.c.6514812.v1

Direct OA link when available
Concepts: Prostate cancer, Tissue microarray, Biomarker, Prostate, Prostatectomy, Chemistry, Medicine, Cancer, Internal medicine, Biochemistry

Top concepts (fields/topics) attached by OpenAlex
Cited by: 0

Total citation count in OpenAlex
Related works (count): 10

Other works algorithmically related by OpenAlex

Full payload

id	https://openalex.org/W4361822718
doi	https://doi.org/10.1158/1055-9965.c.6514812.v1
ids.doi	https://doi.org/10.1158/1055-9965.c.6514812.v1
ids.openalex	https://openalex.org/W4361822718
fwci	0.0
type	preprint
title	Data from Comparative Biomarker Expression and RNA Integrity in Biospecimens Derived from Radical Retropubic and Robot-Assisted Laparoscopic Prostatectomies
biblio.issue
biblio.volume
biblio.last_page
biblio.first_page
topics[0].id	https://openalex.org/T11970
topics[0].field.id	https://openalex.org/fields/13
topics[0].field.display_name	Biochemistry, Genetics and Molecular Biology
topics[0].score	0.9782999753952026
topics[0].domain.id	https://openalex.org/domains/1
topics[0].domain.display_name	Life Sciences
topics[0].subfield.id	https://openalex.org/subfields/1312
topics[0].subfield.display_name	Molecular Biology
topics[0].display_name	Molecular Biology Techniques and Applications
is_xpac	False
apc_list
apc_paid
concepts[0].id	https://openalex.org/C2780192828
concepts[0].level	3
concepts[0].score	0.6462452411651611
concepts[0].wikidata	https://www.wikidata.org/wiki/Q181257
concepts[0].display_name	Prostate cancer
concepts[1].id	https://openalex.org/C193270364
concepts[1].level	3
concepts[1].score	0.5676480531692505
concepts[1].wikidata	https://www.wikidata.org/wiki/Q17141925
concepts[1].display_name	Tissue microarray
concepts[2].id	https://openalex.org/C2781197716
concepts[2].level	2
concepts[2].score	0.5042837858200073
concepts[2].wikidata	https://www.wikidata.org/wiki/Q864574
concepts[2].display_name	Biomarker
concepts[3].id	https://openalex.org/C2776235491
concepts[3].level	3
concepts[3].score	0.4768182635307312
concepts[3].wikidata	https://www.wikidata.org/wiki/Q9625
concepts[3].display_name	Prostate
concepts[4].id	https://openalex.org/C2779466945
concepts[4].level	4
concepts[4].score	0.448900431394577
concepts[4].wikidata	https://www.wikidata.org/wiki/Q1765251
concepts[4].display_name	Prostatectomy
concepts[5].id	https://openalex.org/C185592680
concepts[5].level	0
concepts[5].score	0.39947575330734253
concepts[5].wikidata	https://www.wikidata.org/wiki/Q2329
concepts[5].display_name	Chemistry
concepts[6].id	https://openalex.org/C71924100
concepts[6].level	0
concepts[6].score	0.34997695684432983
concepts[6].wikidata	https://www.wikidata.org/wiki/Q11190
concepts[6].display_name	Medicine
concepts[7].id	https://openalex.org/C121608353
concepts[7].level	2
concepts[7].score	0.31909382343292236
concepts[7].wikidata	https://www.wikidata.org/wiki/Q12078
concepts[7].display_name	Cancer
concepts[8].id	https://openalex.org/C126322002
concepts[8].level	1
concepts[8].score	0.19465035200119019
concepts[8].wikidata	https://www.wikidata.org/wiki/Q11180
concepts[8].display_name	Internal medicine
concepts[9].id	https://openalex.org/C55493867
concepts[9].level	1
concepts[9].score	0.0
concepts[9].wikidata	https://www.wikidata.org/wiki/Q7094
concepts[9].display_name	Biochemistry
keywords[0].id	https://openalex.org/keywords/prostate-cancer
keywords[0].score	0.6462452411651611
keywords[0].display_name	Prostate cancer
keywords[1].id	https://openalex.org/keywords/tissue-microarray
keywords[1].score	0.5676480531692505
keywords[1].display_name	Tissue microarray
keywords[2].id	https://openalex.org/keywords/biomarker
keywords[2].score	0.5042837858200073
keywords[2].display_name	Biomarker
keywords[3].id	https://openalex.org/keywords/prostate
keywords[3].score	0.4768182635307312
keywords[3].display_name	Prostate
keywords[4].id	https://openalex.org/keywords/prostatectomy
keywords[4].score	0.448900431394577
keywords[4].display_name	Prostatectomy
keywords[5].id	https://openalex.org/keywords/chemistry
keywords[5].score	0.39947575330734253
keywords[5].display_name	Chemistry
keywords[6].id	https://openalex.org/keywords/medicine
keywords[6].score	0.34997695684432983
keywords[6].display_name	Medicine
keywords[7].id	https://openalex.org/keywords/cancer
keywords[7].score	0.31909382343292236
keywords[7].display_name	Cancer
keywords[8].id	https://openalex.org/keywords/internal-medicine
keywords[8].score	0.19465035200119019
keywords[8].display_name	Internal medicine
language	en
locations[0].id	doi:10.1158/1055-9965.c.6514812.v1
locations[0].is_oa	True
locations[0].source
locations[0].license	cc-by
locations[0].pdf_url
locations[0].version	acceptedVersion
locations[0].raw_type	posted-content
locations[0].license_id	https://openalex.org/licenses/cc-by
locations[0].is_accepted	True
locations[0].is_published	False
locations[0].raw_source_name
locations[0].landing_page_url	https://doi.org/10.1158/1055-9965.c.6514812.v1
indexed_in	crossref
authorships[0].author.id	https://openalex.org/A5042201449
authorships[0].author.orcid	https://orcid.org/0000-0001-7415-1854
authorships[0].author.display_name	Carmela Ricciardelli
authorships[0].author_position	first
authorships[0].raw_author_name	Carmela Ricciardelli
authorships[0].is_corresponding	False
authorships[1].author.id	https://openalex.org/A5001363437
authorships[1].author.orcid	https://orcid.org/0000-0002-8431-5338
authorships[1].author.display_name	Tina Bianco‐Miotto
authorships[1].author_position	middle
authorships[1].raw_author_name	Tina Bianco-Miotto
authorships[1].is_corresponding	False
authorships[2].author.id	https://openalex.org/A5103698722
authorships[2].author.orcid
authorships[2].author.display_name	Shalini Jindal
authorships[2].author_position	middle
authorships[2].raw_author_name	Shalini Jindal
authorships[2].is_corresponding	False
authorships[3].author.id	https://openalex.org/A5090088695
authorships[3].author.orcid
authorships[3].author.display_name	Thomas J. Dodd
authorships[3].author_position	middle
authorships[3].raw_author_name	Thomas J. Dodd
authorships[3].is_corresponding	False
authorships[4].author.id	https://openalex.org/A5051897586
authorships[4].author.orcid
authorships[4].author.display_name	Penelope Cohen
authorships[4].author_position	middle
authorships[4].raw_author_name	Penelope A. Cohen
authorships[4].is_corresponding	False
authorships[5].author.id	https://openalex.org/A5087150215
authorships[5].author.orcid
authorships[5].author.display_name	Villis R. Marshall
authorships[5].author_position	middle
authorships[5].raw_author_name	Villis R. Marshall
authorships[5].is_corresponding	False
authorships[6].author.id	https://openalex.org/A5053731371
authorships[6].author.orcid	https://orcid.org/0000-0003-1893-9203
authorships[6].author.display_name	Peter Sutherland
authorships[6].author_position	middle
authorships[6].raw_author_name	Peter D. Sutherland
authorships[6].is_corresponding	False
authorships[7].author.id	https://openalex.org/A5045527532
authorships[7].author.orcid	https://orcid.org/0000-0001-5796-1791
authorships[7].author.display_name	Hemamali Samaratunga
authorships[7].author_position	middle
authorships[7].raw_author_name	Hemamali Samaratunga
authorships[7].is_corresponding	False
authorships[8].author.id	https://openalex.org/A5086908096
authorships[8].author.orcid	https://orcid.org/0000-0001-8687-4988
authorships[8].author.display_name	James G. Kench
authorships[8].author_position	middle
authorships[8].raw_author_name	James G. Kench
authorships[8].is_corresponding	False
authorships[9].author.id	https://openalex.org/A5113543953
authorships[9].author.orcid	https://orcid.org/0000-0002-7367-8862
authorships[9].author.display_name	Ying Dong
authorships[9].author_position	middle
authorships[9].raw_author_name	Ying Dong
authorships[9].is_corresponding	False
authorships[10].author.id	https://openalex.org/A5100370911
authorships[10].author.orcid	https://orcid.org/0000-0002-2754-7052
authorships[10].author.display_name	Hong Wang
authorships[10].author_position	middle
authorships[10].raw_author_name	Hong Wang
authorships[10].is_corresponding	False
authorships[11].author.id	https://openalex.org/A5028698179
authorships[11].author.orcid	https://orcid.org/0000-0001-6026-1964
authorships[11].author.display_name	Judith A. Clements
authorships[11].author_position	middle
authorships[11].raw_author_name	Judith A. Clements
authorships[11].is_corresponding	False
authorships[12].author.id	https://openalex.org/A5089881929
authorships[12].author.orcid	https://orcid.org/0000-0003-3089-4028
authorships[12].author.display_name	Gail P. Risbridger
authorships[12].author_position	middle
authorships[12].raw_author_name	Gail P. Risbridger
authorships[12].is_corresponding	False
authorships[13].author.id	https://openalex.org/A5110319158
authorships[13].author.orcid
authorships[13].author.display_name	Robert L. Sutherland
authorships[13].author_position	middle
authorships[13].raw_author_name	Robert L. Sutherland
authorships[13].is_corresponding	False
authorships[14].author.id	https://openalex.org/A5020285542
authorships[14].author.orcid	https://orcid.org/0000-0003-1893-2626
authorships[14].author.display_name	Wayne D. Tilley
authorships[14].author_position	middle
authorships[14].raw_author_name	Wayne D. Tilley
authorships[14].is_corresponding	False
authorships[15].author.id	https://openalex.org/A5113867086
authorships[15].author.orcid
authorships[15].author.display_name	David J. Horsfall
authorships[15].author_position	last
authorships[15].raw_author_name	David J. Horsfall
authorships[15].is_corresponding	False
has_content.pdf	False
has_content.grobid_xml	False
is_paratext	False
open_access.is_oa	True
open_access.oa_url	https://doi.org/10.1158/1055-9965.c.6514812.v1
open_access.oa_status	gold
open_access.any_repository_has_fulltext	False
created_date	2025-10-10T00:00:00
display_name	Data from Comparative Biomarker Expression and RNA Integrity in Biospecimens Derived from Radical Retropubic and Robot-Assisted Laparoscopic Prostatectomies
has_fulltext	False
is_retracted	False
updated_date	2025-11-06T03:46:38.306776
primary_topic.id	https://openalex.org/T11970
primary_topic.field.id	https://openalex.org/fields/13
primary_topic.field.display_name	Biochemistry, Genetics and Molecular Biology
primary_topic.score	0.9782999753952026
primary_topic.domain.id	https://openalex.org/domains/1
primary_topic.domain.display_name	Life Sciences
primary_topic.subfield.id	https://openalex.org/subfields/1312
primary_topic.subfield.display_name	Molecular Biology
primary_topic.display_name	Molecular Biology Techniques and Applications
related_works	https://openalex.org/W2362764965, https://openalex.org/W2355155998, https://openalex.org/W2023475404, https://openalex.org/W2066092646, https://openalex.org/W2088520467, https://openalex.org/W2075763133, https://openalex.org/W2376423713, https://openalex.org/W1583600832, https://openalex.org/W2006203624, https://openalex.org/W1978302297
cited_by_count	0
locations_count	1
best_oa_location.id	doi:10.1158/1055-9965.c.6514812.v1
best_oa_location.is_oa	True
best_oa_location.source
best_oa_location.license	cc-by
best_oa_location.pdf_url
best_oa_location.version	acceptedVersion
best_oa_location.raw_type	posted-content
best_oa_location.license_id	https://openalex.org/licenses/cc-by
best_oa_location.is_accepted	True
best_oa_location.is_published	False
best_oa_location.raw_source_name
best_oa_location.landing_page_url	https://doi.org/10.1158/1055-9965.c.6514812.v1
primary_location.id	doi:10.1158/1055-9965.c.6514812.v1
primary_location.is_oa	True
primary_location.source
primary_location.license	cc-by
primary_location.pdf_url
primary_location.version	acceptedVersion
primary_location.raw_type	posted-content
primary_location.license_id	https://openalex.org/licenses/cc-by
primary_location.is_accepted	True
primary_location.is_published	False
primary_location.raw_source_name
primary_location.landing_page_url	https://doi.org/10.1158/1055-9965.c.6514812.v1
publication_date	2023-03-31
publication_year	2023
referenced_works_count	0
abstract_inverted_index.14	95
abstract_inverted_index.22	76
abstract_inverted_index.23	73
abstract_inverted_index.No	139, 188
abstract_inverted_index.We	35
abstract_inverted_index.as	30, 91
abstract_inverted_index.at	213
abstract_inverted_index.be	245
abstract_inverted_index.by	43
abstract_inverted_index.in	38, 168, 182, 190, 204, 229
abstract_inverted_index.is	10, 221
abstract_inverted_index.no	166
abstract_inverted_index.of	2, 51, 69, 72, 99, 239
abstract_inverted_index.on	127
abstract_inverted_index.or	26, 165, 193
abstract_inverted_index.to	9, 83, 102
abstract_inverted_index.PCR	116
abstract_inverted_index.RNA	39, 128, 191
abstract_inverted_index.RRP	77, 135, 147, 186, 201, 210, 242
abstract_inverted_index.and	20, 47, 63, 75, 87, 94, 125, 133, 146, 179, 200, 209, 236, 241
abstract_inverted_index.are	7
abstract_inverted_index.but	218
abstract_inverted_index.for	32, 119, 227
abstract_inverted_index.the	106, 214, 219
abstract_inverted_index.was	117, 196
abstract_inverted_index.(AR)	86
abstract_inverted_index.(AR,	170
abstract_inverted_index.PCR,	46
abstract_inverted_index.RALP	74, 132, 145, 183, 199, 208, 234, 240
abstract_inverted_index.YB1,	176
abstract_inverted_index.done	118
abstract_inverted_index.five	131, 134
abstract_inverted_index.from	58, 130
abstract_inverted_index.gene	41, 194
abstract_inverted_index.into	154
abstract_inverted_index.p63,	177
abstract_inverted_index.some	33
abstract_inverted_index.that	5
abstract_inverted_index.were	79, 142
abstract_inverted_index.when	232
abstract_inverted_index.with	81, 156, 185
abstract_inverted_index.(PSA)	90
abstract_inverted_index.(PSA,	158
abstract_inverted_index.(RRP)	62
abstract_inverted_index.Ki67,	172
abstract_inverted_index.PAR2,	175
abstract_inverted_index.PCNA,	173
abstract_inverted_index.Prev;	250
abstract_inverted_index.exist	212
abstract_inverted_index.might	22
abstract_inverted_index.mixed	237
abstract_inverted_index.novel	14
abstract_inverted_index.other	96
abstract_inverted_index.these	152
abstract_inverted_index.those	155
abstract_inverted_index.three	103
abstract_inverted_index.using	233
abstract_inverted_index.(KLK2,	163
abstract_inverted_index.19(7);	251
abstract_inverted_index.CKHMW,	160
abstract_inverted_index.Cancer	109, 247
abstract_inverted_index.Future	223
abstract_inverted_index.KLK4),	161
abstract_inverted_index.affect	23
abstract_inverted_index.cancer	54
abstract_inverted_index.change	167
abstract_inverted_index.frozen	136
abstract_inverted_index.level,	217
abstract_inverted_index.should	225, 244
abstract_inverted_index.tissue	17, 28, 70, 111, 137, 211, 243
abstract_inverted_index.within	105
abstract_inverted_index.©2010	253
abstract_inverted_index.(RALP).	67
abstract_inverted_index.CK8/18,	159
abstract_inverted_index.Changes	203
abstract_inverted_index.KLK14),	164
abstract_inverted_index.VEGF-C,	174
abstract_inverted_index.account	226
abstract_inverted_index.antigen	89
abstract_inverted_index.banking	112
abstract_inverted_index.because	13
abstract_inverted_index.between	56, 144, 198, 207
abstract_inverted_index.changes	228
abstract_inverted_index.cohorts	238
abstract_inverted_index.grouped	151
abstract_inverted_index.protein	49, 216
abstract_inverted_index.quality	192
abstract_inverted_index.radical	60
abstract_inverted_index.samples	29, 78, 153
abstract_inverted_index.stained	80
abstract_inverted_index.storage	21
abstract_inverted_index.studies	224
abstract_inverted_index.tissue.	148, 187
abstract_inverted_index.tissues	57
abstract_inverted_index.Prostate	108
abstract_inverted_index.Sections	68
abstract_inverted_index.analytes	31
abstract_inverted_index.androgen	84
abstract_inverted_index.avoided.	246
abstract_inverted_index.compared	184
abstract_inverted_index.detected	197
abstract_inverted_index.etiology	220
abstract_inverted_index.ghrelin,	171
abstract_inverted_index.interest	101
abstract_inverted_index.network.	113
abstract_inverted_index.observed	143
abstract_inverted_index.prostate	53
abstract_inverted_index.quality,	40
abstract_inverted_index.receptor	85
abstract_inverted_index.research	100
abstract_inverted_index.selected	52
abstract_inverted_index.staining	150
abstract_inverted_index.surgical	15
abstract_inverted_index.tissues,	235
abstract_inverted_index.Biomarker	149
abstract_inverted_index.Epidemiol	248
abstract_inverted_index.Knowledge	1
abstract_inverted_index.biomarker	24, 205, 230
abstract_inverted_index.candidate	97
abstract_inverted_index.controls,	93
abstract_inverted_index.decreased	162
abstract_inverted_index.extracted	129
abstract_inverted_index.handling,	19
abstract_inverted_index.important	12
abstract_inverted_index.increased	157
abstract_inverted_index.intersite	92
abstract_inverted_index.real-time	45, 115
abstract_inverted_index.sampling,	18
abstract_inverted_index.subjected	8
abstract_inverted_index.versican,	178
abstract_inverted_index.0-sulfate)	181
abstract_inverted_index.1755–65.	252
abstract_inverted_index.Australian	107
abstract_inverted_index.Biomarkers	249
abstract_inverted_index.antibodies	82
abstract_inverted_index.biomarkers	55, 98
abstract_inverted_index.conditions	4
abstract_inverted_index.critically	11
abstract_inverted_index.difference	189
abstract_inverted_index.expression	25, 42, 50, 169, 195, 206, 231
abstract_inverted_index.histologic	140
abstract_inverted_index.invalidate	27
abstract_inverted_index.microarray	71
abstract_inverted_index.retropubic	59
abstract_inverted_index.BioResource	110
abstract_inverted_index.chondroitin	180
abstract_inverted_index.differences	37, 141
abstract_inverted_index.preanalytic	3
abstract_inverted_index.procedures,	16
abstract_inverted_index.<i>AR,	120
abstract_inverted_index.Quantitative	114
abstract_inverted_index.biospecimens	6
abstract_inverted_index.investigated	36
abstract_inverted_index.laboratories	104
abstract_inverted_index.laparoscopic	65
abstract_inverted_index.quantitative	44
abstract_inverted_index.PSA</i>	121
abstract_inverted_index.prostatectomy	61, 66
abstract_inverted_index.<i>KLK2,	123
abstract_inverted_index.immunoreactive	48, 215
abstract_inverted_index.robot-assisted	64
abstract_inverted_index.KLK4</i>,	124
abstract_inverted_index.prostate-specific	88
abstract_inverted_index.<i>HIF1A</i>	126
abstract_inverted_index.(<i>KLK3</i>),	122
abstract_inverted_index.AACR.</p></div>	254
abstract_inverted_index.cores.</p><p><b>Results:</b>	138
abstract_inverted_index.unclear.</p><p><b>Impact:</b>	222
abstract_inverted_index.tissue.</p><p><b>Conclusions:</b>	202
abstract_inverted_index.<div>Abstract<p><b>Background:</b>	0
abstract_inverted_index.technologies.</p><p><b>Methods:</b>	34
cited_by_percentile_year
countries_distinct_count	0
institutions_distinct_count	16
citation_normalized_percentile.value	0.06239943
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False