Data from: Real-time imaging of rotation during synthesis by the replisome Article Swipe

View

YOU? · · 2025 · Open Access · · DOI: https://doi.org/10.5061/dryad.j0zpc86rc

During chromosome replication, unwinding by the helicase and synthesis by the polymerases can lead to overwinding and supercoiling of DNA. The mechanical consequences of these events and resulting local dynamics at the replication fork are not well understood. To address these issues, we developed a transverse DNA flow-stretching approach to spatially resolve the parental, leading, and lagging strands in real-time. Using bacteriophage T7 as a model system, this approach revealed bursts of high-speed replisome rotation that support continuous DNA synthesis. Surprisingly, excessive rotation does not reduce replisome speed, but increases pausing, reduces processivity, and increases the number of polymerases. Taken together, our observations reveal intrinsic pathways to overcome challenges posed by unfavorable DNA topologies during DNA replication.

Related Topics

Concepts

Replisome Rotation (mathematics) Physics DNA polymerase Helicase DNA DNA replication Circular bacterial chromosome DNA supercoil Biology Coupling (piping) Biophysics Topology (electrical circuits) A-DNA Biological system DNA synthesis Magnetic tweezers Genetics

Metadata

Type: dataset
Language: en
Landing Page: https://doi.org/10.5061/dryad.j0zpc86rc
OA Status: green
OpenAlex ID: https://openalex.org/W7105925207

All OpenAlex metadata

Raw OpenAlex JSON

OpenAlex ID: https://openalex.org/W7105925207

Canonical identifier for this work in OpenAlex
DOI: https://doi.org/10.5061/dryad.j0zpc86rc

Digital Object Identifier
Title: Data from: Real-time imaging of rotation during synthesis by the replisome

Work title
Type: dataset

OpenAlex work type
Language: en

Primary language
Publication year: 2025

Year of publication
Publication date: 2025-11-05

Full publication date if available
Authors: Retzer, Thomas, Duderstadt, Karl

List of authors in order
Landing page: https://doi.org/10.5061/dryad.j0zpc86rc

Publisher landing page
Open access: Yes

Whether a free full text is available
OA status: green

Open access status per OpenAlex
OA URL: https://doi.org/10.5061/dryad.j0zpc86rc

Direct OA link when available
Concepts: Replisome, Rotation (mathematics), Physics, DNA polymerase, Helicase, DNA, DNA replication, Circular bacterial chromosome, DNA supercoil, Biology, Coupling (piping), Biophysics, Topology (electrical circuits), A-DNA, Biological system, DNA synthesis, Magnetic tweezers, Genetics

Top concepts (fields/topics) attached by OpenAlex
Cited by: 0

Total citation count in OpenAlex

Full payload

id	https://openalex.org/W7105925207
doi	https://doi.org/10.5061/dryad.j0zpc86rc
ids.doi	https://doi.org/10.5061/dryad.j0zpc86rc
ids.openalex	https://openalex.org/W7105925207
fwci
type	dataset
title	Data from: Real-time imaging of rotation during synthesis by the replisome
biblio.issue
biblio.volume
biblio.last_page
biblio.first_page
is_xpac	False
apc_list
apc_paid
concepts[0].id	https://openalex.org/C149187182
concepts[0].level	5
concepts[0].score	0.9840427041053772
concepts[0].wikidata	https://www.wikidata.org/wiki/Q22325217
concepts[0].display_name	Replisome
concepts[1].id	https://openalex.org/C74050887
concepts[1].level	2
concepts[1].score	0.5882542133331299
concepts[1].wikidata	https://www.wikidata.org/wiki/Q848368
concepts[1].display_name	Rotation (mathematics)
concepts[2].id	https://openalex.org/C121332964
concepts[2].level	0
concepts[2].score	0.5622890591621399
concepts[2].wikidata	https://www.wikidata.org/wiki/Q413
concepts[2].display_name	Physics
concepts[3].id	https://openalex.org/C2756471
concepts[3].level	3
concepts[3].score	0.5277902483940125
concepts[3].wikidata	https://www.wikidata.org/wiki/Q206286
concepts[3].display_name	DNA polymerase
concepts[4].id	https://openalex.org/C161223559
concepts[4].level	4
concepts[4].score	0.5008739233016968
concepts[4].wikidata	https://www.wikidata.org/wiki/Q138864
concepts[4].display_name	Helicase
concepts[5].id	https://openalex.org/C552990157
concepts[5].level	2
concepts[5].score	0.4998149275779724
concepts[5].wikidata	https://www.wikidata.org/wiki/Q7430
concepts[5].display_name	DNA
concepts[6].id	https://openalex.org/C73573662
concepts[6].level	3
concepts[6].score	0.4931517541408539
concepts[6].wikidata	https://www.wikidata.org/wiki/Q130996
concepts[6].display_name	DNA replication
concepts[7].id	https://openalex.org/C58167604
concepts[7].level	4
concepts[7].score	0.491873174905777
concepts[7].wikidata	https://www.wikidata.org/wiki/Q5121654
concepts[7].display_name	Circular bacterial chromosome
concepts[8].id	https://openalex.org/C158456008
concepts[8].level	4
concepts[8].score	0.4777708351612091
concepts[8].wikidata	https://www.wikidata.org/wiki/Q412639
concepts[8].display_name	DNA supercoil
concepts[9].id	https://openalex.org/C86803240
concepts[9].level	0
concepts[9].score	0.4193321764469147
concepts[9].wikidata	https://www.wikidata.org/wiki/Q420
concepts[9].display_name	Biology
concepts[10].id	https://openalex.org/C131584629
concepts[10].level	2
concepts[10].score	0.4057861864566803
concepts[10].wikidata	https://www.wikidata.org/wiki/Q4308705
concepts[10].display_name	Coupling (piping)
concepts[11].id	https://openalex.org/C12554922
concepts[11].level	1
concepts[11].score	0.3439638316631317
concepts[11].wikidata	https://www.wikidata.org/wiki/Q7100
concepts[11].display_name	Biophysics
concepts[12].id	https://openalex.org/C184720557
concepts[12].level	2
concepts[12].score	0.3341066241264343
concepts[12].wikidata	https://www.wikidata.org/wiki/Q7825049
concepts[12].display_name	Topology (electrical circuits)
concepts[13].id	https://openalex.org/C191961535
concepts[13].level	3
concepts[13].score	0.3008469045162201
concepts[13].wikidata	https://www.wikidata.org/wiki/Q2360205
concepts[13].display_name	A-DNA
concepts[14].id	https://openalex.org/C186060115
concepts[14].level	1
concepts[14].score	0.28659722208976746
concepts[14].wikidata	https://www.wikidata.org/wiki/Q30336093
concepts[14].display_name	Biological system
concepts[15].id	https://openalex.org/C64241487
concepts[15].level	3
concepts[15].score	0.28613707423210144
concepts[15].wikidata	https://www.wikidata.org/wiki/Q5205774
concepts[15].display_name	DNA synthesis
concepts[16].id	https://openalex.org/C105480513
concepts[16].level	3
concepts[16].score	0.2536976933479309
concepts[16].wikidata	https://www.wikidata.org/wiki/Q6731630
concepts[16].display_name	Magnetic tweezers
concepts[17].id	https://openalex.org/C54355233
concepts[17].level	1
concepts[17].score	0.25227805972099304
concepts[17].wikidata	https://www.wikidata.org/wiki/Q7162
concepts[17].display_name	Genetics
keywords[0].id	https://openalex.org/keywords/replisome
keywords[0].score	0.9840427041053772
keywords[0].display_name	Replisome
keywords[1].id	https://openalex.org/keywords/rotation
keywords[1].score	0.5882542133331299
keywords[1].display_name	Rotation (mathematics)
keywords[2].id	https://openalex.org/keywords/dna-polymerase
keywords[2].score	0.5277902483940125
keywords[2].display_name	DNA polymerase
keywords[3].id	https://openalex.org/keywords/helicase
keywords[3].score	0.5008739233016968
keywords[3].display_name	Helicase
keywords[4].id	https://openalex.org/keywords/dna
keywords[4].score	0.4998149275779724
keywords[4].display_name	DNA
keywords[5].id	https://openalex.org/keywords/dna-replication
keywords[5].score	0.4931517541408539
keywords[5].display_name	DNA replication
keywords[6].id	https://openalex.org/keywords/circular-bacterial-chromosome
keywords[6].score	0.491873174905777
keywords[6].display_name	Circular bacterial chromosome
language	en
locations[0].id	doi:10.5061/dryad.j0zpc86rc
locations[0].is_oa	True
locations[0].source.id	https://openalex.org/S7407051161
locations[0].source.type	repository
locations[0].source.is_oa	False
locations[0].source.issn_l
locations[0].source.is_core	False
locations[0].source.is_in_doaj	False
locations[0].source.display_name	DRYAD
locations[0].source.host_organization
locations[0].source.host_organization_name
locations[0].license	public-domain
locations[0].pdf_url
locations[0].version
locations[0].raw_type	dataset
locations[0].license_id	https://openalex.org/licenses/public-domain
locations[0].is_accepted	False
locations[0].is_published
locations[0].raw_source_name
locations[0].landing_page_url	https://doi.org/10.5061/dryad.j0zpc86rc
indexed_in	datacite
authorships[0].author.id
authorships[0].author.orcid
authorships[0].author.display_name	Retzer, Thomas
authorships[0].countries	DE
authorships[0].affiliations[0].institution_ids	https://openalex.org/I62916508
authorships[0].affiliations[0].raw_affiliation_string	Technical University of Munich
authorships[0].institutions[0].id	https://openalex.org/I62916508
authorships[0].institutions[0].ror	https://ror.org/02kkvpp62
authorships[0].institutions[0].type	education
authorships[0].institutions[0].lineage	https://openalex.org/I62916508
authorships[0].institutions[0].country_code	DE
authorships[0].institutions[0].display_name	Technical University of Munich
authorships[0].author_position	first
authorships[0].raw_author_name	Retzer, Thomas
authorships[0].is_corresponding	True
authorships[0].raw_affiliation_strings	Technical University of Munich
authorships[1].author.id
authorships[1].author.orcid
authorships[1].author.display_name	Duderstadt, Karl
authorships[1].countries	DE
authorships[1].affiliations[0].institution_ids	https://openalex.org/I62916508
authorships[1].affiliations[0].raw_affiliation_string	Technical University of Munich
authorships[1].institutions[0].id	https://openalex.org/I62916508
authorships[1].institutions[0].ror	https://ror.org/02kkvpp62
authorships[1].institutions[0].type	education
authorships[1].institutions[0].lineage	https://openalex.org/I62916508
authorships[1].institutions[0].country_code	DE
authorships[1].institutions[0].display_name	Technical University of Munich
authorships[1].author_position	last
authorships[1].raw_author_name	Duderstadt, Karl
authorships[1].is_corresponding	False
authorships[1].raw_affiliation_strings	Technical University of Munich
has_content.pdf	False
has_content.grobid_xml	False
is_paratext	False
open_access.is_oa	True
open_access.oa_url	https://doi.org/10.5061/dryad.j0zpc86rc
open_access.oa_status	green
open_access.any_repository_has_fulltext	False
created_date	2025-11-18T00:00:00
display_name	Data from: Real-time imaging of rotation during synthesis by the replisome
has_fulltext	False
is_retracted	False
updated_date	2025-11-18T23:46:17.205004
primary_topic
cited_by_count	0
locations_count	1
best_oa_location.id	doi:10.5061/dryad.j0zpc86rc
best_oa_location.is_oa	True
best_oa_location.source.id	https://openalex.org/S7407051161
best_oa_location.source.type	repository
best_oa_location.source.is_oa	False
best_oa_location.source.issn_l
best_oa_location.source.is_core	False
best_oa_location.source.is_in_doaj	False
best_oa_location.source.display_name	DRYAD
best_oa_location.source.host_organization
best_oa_location.source.host_organization_name
best_oa_location.license	public-domain
best_oa_location.pdf_url
best_oa_location.version
best_oa_location.raw_type	dataset
best_oa_location.license_id	https://openalex.org/licenses/public-domain
best_oa_location.is_accepted	False
best_oa_location.is_published	False
best_oa_location.raw_source_name
best_oa_location.landing_page_url	https://doi.org/10.5061/dryad.j0zpc86rc
primary_location.id	doi:10.5061/dryad.j0zpc86rc
primary_location.is_oa	True
primary_location.source.id	https://openalex.org/S7407051161
primary_location.source.type	repository
primary_location.source.is_oa	False
primary_location.source.issn_l
primary_location.source.is_core	False
primary_location.source.is_in_doaj	False
primary_location.source.display_name	DRYAD
primary_location.source.host_organization
primary_location.source.host_organization_name
primary_location.license	public-domain
primary_location.pdf_url
primary_location.version
primary_location.raw_type	dataset
primary_location.license_id	https://openalex.org/licenses/public-domain
primary_location.is_accepted	False
primary_location.is_published	False
primary_location.raw_source_name
primary_location.landing_page_url	https://doi.org/10.5061/dryad.j0zpc86rc
publication_date	2025-11-05
publication_year	2025
referenced_works_count	0
abstract_inverted_index.a	44, 64
abstract_inverted_index.T7	62
abstract_inverted_index.To	38
abstract_inverted_index.as	63
abstract_inverted_index.at	30
abstract_inverted_index.by	4, 9, 110
abstract_inverted_index.in	58
abstract_inverted_index.of	18, 23, 71, 97
abstract_inverted_index.to	14, 49, 106
abstract_inverted_index.we	42
abstract_inverted_index.DNA	46, 78, 112, 115
abstract_inverted_index.The	20
abstract_inverted_index.and	7, 16, 26, 55, 93
abstract_inverted_index.are	34
abstract_inverted_index.but	88
abstract_inverted_index.can	12
abstract_inverted_index.not	35, 84
abstract_inverted_index.our	101
abstract_inverted_index.the	5, 10, 31, 52, 95
abstract_inverted_index.DNA.	19
abstract_inverted_index.does	83
abstract_inverted_index.fork	33
abstract_inverted_index.lead	13
abstract_inverted_index.that	75
abstract_inverted_index.this	67
abstract_inverted_index.well	36
abstract_inverted_index.Taken	99
abstract_inverted_index.Using	60
abstract_inverted_index.local	28
abstract_inverted_index.model	65
abstract_inverted_index.posed	109
abstract_inverted_index.these	24, 40
abstract_inverted_index.During	0
abstract_inverted_index.bursts	70
abstract_inverted_index.during	114
abstract_inverted_index.events	25
abstract_inverted_index.number	96
abstract_inverted_index.reduce	85
abstract_inverted_index.reveal	103
abstract_inverted_index.speed,	87
abstract_inverted_index.address	39
abstract_inverted_index.issues,	41
abstract_inverted_index.lagging	56
abstract_inverted_index.reduces	91
abstract_inverted_index.resolve	51
abstract_inverted_index.strands	57
abstract_inverted_index.support	76
abstract_inverted_index.system,	66
abstract_inverted_index.approach	48, 68
abstract_inverted_index.dynamics	29
abstract_inverted_index.helicase	6
abstract_inverted_index.leading,	54
abstract_inverted_index.overcome	107
abstract_inverted_index.pathways	105
abstract_inverted_index.pausing,	90
abstract_inverted_index.revealed	69
abstract_inverted_index.rotation	74, 82
abstract_inverted_index.developed	43
abstract_inverted_index.excessive	81
abstract_inverted_index.increases	89, 94
abstract_inverted_index.intrinsic	104
abstract_inverted_index.parental,	53
abstract_inverted_index.replisome	73, 86
abstract_inverted_index.resulting	27
abstract_inverted_index.spatially	50
abstract_inverted_index.synthesis	8
abstract_inverted_index.together,	100
abstract_inverted_index.unwinding	3
abstract_inverted_index.challenges	108
abstract_inverted_index.chromosome	1
abstract_inverted_index.continuous	77
abstract_inverted_index.high-speed	72
abstract_inverted_index.mechanical	21
abstract_inverted_index.real-time.	59
abstract_inverted_index.synthesis.	79
abstract_inverted_index.topologies	113
abstract_inverted_index.transverse	45
abstract_inverted_index.overwinding	15
abstract_inverted_index.polymerases	11
abstract_inverted_index.replication	32
abstract_inverted_index.understood.	37
abstract_inverted_index.unfavorable	111
abstract_inverted_index.consequences	22
abstract_inverted_index.observations	102
abstract_inverted_index.polymerases.	98
abstract_inverted_index.replication,	2
abstract_inverted_index.replication.	116
abstract_inverted_index.supercoiling	17
abstract_inverted_index.Surprisingly,	80
abstract_inverted_index.bacteriophage	61
abstract_inverted_index.processivity,	92
abstract_inverted_index.flow-stretching	47
cited_by_percentile_year
countries_distinct_count	1
institutions_distinct_count	2
citation_normalized_percentile