Deciphering the structural intricacy in virulence effectors for proton-motive force mediated unfolding and type-III protein secretion Article Swipe
Basavraj Khanppnavar
,
Anupam Roy
,
Kousik Chandra
,
Nakul C. Maiti
,
Saumen Datta
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1101/557496
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.1101/557496
Many gram-negative pathogenic bacteria use type III secretion system (T3SS) to inject virulence effectors directly into the cytosol of targeted host cells. Given that the protein unfolding requisite for secretion via nano-size pore of T3SS injectisome is an energetically unfavorable process, “How do pathogenic bacteria unfold and secrete hundreds of toxic proteins in seconds” remain largely unknown. In this study, first, from an in-depth analysis of folding and stability of T3SS effector ExoY, we show that the proton-concentration gradient (∼pH 5.8-6.0) generated by proton-motive force (PMF) can significantly amortize tertiary structural folding and stability of effectors without significant entropic cost. Strikingly, it was found that the lower energetic cost associated with the global unfolding of ExoY is mainly due to its weakly folded geometry and abundance of geometrical frustrations stemming from buried water molecules and native-like folded intermediates in the folded cores. From in-silico structural analysis of 371 T3SS effectors, it can be curtained that T3SS effectors belong to typical class (disorder globules) of IDPs and have evolved similar conserved intrinsic structural archetypes to mediate early-stage unfolding. The slower folding kinetics in effector proteins requisite for efficient T3SS-mediated secretion mostly stems from reduced hydrophobic density and enhanced polar-polar repulsive interactions in their sequence landscapes. Lastly, the positively evolved histidine-mediated stabilizing interactions and gate-keeper residues in effector proteins shed light on collaborative role of evolved structural chemistry in T3SS effectors and PMF in the spatial-temporal regulation of effector folding and stability essential for maintaining balance in secretion and function trade-off.
Related Topics
Concepts
Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/557496
- https://www.biorxiv.org/content/biorxiv/early/2019/02/21/557496.full.pdf
- OA Status
- green
- References
- 70
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2915714513
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W2915714513Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1101/557496Digital Object Identifier
- Title
-
Deciphering the structural intricacy in virulence effectors for proton-motive force mediated unfolding and type-III protein secretionWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2019Year of publication
- Publication date
-
2019-02-21Full publication date if available
- Authors
-
Basavraj Khanppnavar, Anupam Roy, Kousik Chandra, Nakul C. Maiti, Saumen DattaList of authors in order
- Landing page
-
https://doi.org/10.1101/557496Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2019/02/21/557496.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2019/02/21/557496.full.pdfDirect OA link when available
- Concepts
-
Effector, Secretion, Folding (DSP implementation), Type three secretion system, Virulence, Protein folding, Biophysics, Chemistry, Protein structure, Biology, Biochemistry, Engineering, Electrical engineering, GeneTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
0Total citation count in OpenAlex
- References (count)
-
70Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W2915714513 |
|---|---|
| doi | https://doi.org/10.1101/557496 |
| ids.doi | https://doi.org/10.1101/557496 |
| ids.mag | 2915714513 |
| ids.openalex | https://openalex.org/W2915714513 |
| fwci | 0.0 |
| type | preprint |
| title | Deciphering the structural intricacy in virulence effectors for proton-motive force mediated unfolding and type-III protein secretion |
| biblio.issue | |
| biblio.volume | |
| biblio.last_page | |
| biblio.first_page | |
| topics[0].id | https://openalex.org/T10044 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.9980999827384949 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1312 |
| topics[0].subfield.display_name | Molecular Biology |
| topics[0].display_name | Protein Structure and Dynamics |
| topics[1].id | https://openalex.org/T12232 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9977999925613403 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1311 |
| topics[1].subfield.display_name | Genetics |
| topics[1].display_name | Yersinia bacterium, plague, ectoparasites research |
| topics[2].id | https://openalex.org/T11048 |
| topics[2].field.id | https://openalex.org/fields/23 |
| topics[2].field.display_name | Environmental Science |
| topics[2].score | 0.9976999759674072 |
| topics[2].domain.id | https://openalex.org/domains/3 |
| topics[2].domain.display_name | Physical Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2303 |
| topics[2].subfield.display_name | Ecology |
| topics[2].display_name | Bacteriophages and microbial interactions |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C51785407 |
| concepts[0].level | 2 |
| concepts[0].score | 0.9201617240905762 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q2627568 |
| concepts[0].display_name | Effector |
| concepts[1].id | https://openalex.org/C49039625 |
| concepts[1].level | 2 |
| concepts[1].score | 0.6925221681594849 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q84230 |
| concepts[1].display_name | Secretion |
| concepts[2].id | https://openalex.org/C2776545253 |
| concepts[2].level | 2 |
| concepts[2].score | 0.5941622257232666 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q5464292 |
| concepts[2].display_name | Folding (DSP implementation) |
| concepts[3].id | https://openalex.org/C130211564 |
| concepts[3].level | 4 |
| concepts[3].score | 0.554283857345581 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q21199165 |
| concepts[3].display_name | Type three secretion system |
| concepts[4].id | https://openalex.org/C60987743 |
| concepts[4].level | 3 |
| concepts[4].score | 0.525782585144043 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q1460232 |
| concepts[4].display_name | Virulence |
| concepts[5].id | https://openalex.org/C204328495 |
| concepts[5].level | 2 |
| concepts[5].score | 0.5005500316619873 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q847556 |
| concepts[5].display_name | Protein folding |
| concepts[6].id | https://openalex.org/C12554922 |
| concepts[6].level | 1 |
| concepts[6].score | 0.4807543456554413 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q7100 |
| concepts[6].display_name | Biophysics |
| concepts[7].id | https://openalex.org/C185592680 |
| concepts[7].level | 0 |
| concepts[7].score | 0.4626171886920929 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[7].display_name | Chemistry |
| concepts[8].id | https://openalex.org/C47701112 |
| concepts[8].level | 2 |
| concepts[8].score | 0.4287511706352234 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q735188 |
| concepts[8].display_name | Protein structure |
| concepts[9].id | https://openalex.org/C86803240 |
| concepts[9].level | 0 |
| concepts[9].score | 0.35113656520843506 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[9].display_name | Biology |
| concepts[10].id | https://openalex.org/C55493867 |
| concepts[10].level | 1 |
| concepts[10].score | 0.21067258715629578 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[10].display_name | Biochemistry |
| concepts[11].id | https://openalex.org/C127413603 |
| concepts[11].level | 0 |
| concepts[11].score | 0.0 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q11023 |
| concepts[11].display_name | Engineering |
| concepts[12].id | https://openalex.org/C119599485 |
| concepts[12].level | 1 |
| concepts[12].score | 0.0 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q43035 |
| concepts[12].display_name | Electrical engineering |
| concepts[13].id | https://openalex.org/C104317684 |
| concepts[13].level | 2 |
| concepts[13].score | 0.0 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[13].display_name | Gene |
| keywords[0].id | https://openalex.org/keywords/effector |
| keywords[0].score | 0.9201617240905762 |
| keywords[0].display_name | Effector |
| keywords[1].id | https://openalex.org/keywords/secretion |
| keywords[1].score | 0.6925221681594849 |
| keywords[1].display_name | Secretion |
| keywords[2].id | https://openalex.org/keywords/folding |
| keywords[2].score | 0.5941622257232666 |
| keywords[2].display_name | Folding (DSP implementation) |
| keywords[3].id | https://openalex.org/keywords/type-three-secretion-system |
| keywords[3].score | 0.554283857345581 |
| keywords[3].display_name | Type three secretion system |
| keywords[4].id | https://openalex.org/keywords/virulence |
| keywords[4].score | 0.525782585144043 |
| keywords[4].display_name | Virulence |
| keywords[5].id | https://openalex.org/keywords/protein-folding |
| keywords[5].score | 0.5005500316619873 |
| keywords[5].display_name | Protein folding |
| keywords[6].id | https://openalex.org/keywords/biophysics |
| keywords[6].score | 0.4807543456554413 |
| keywords[6].display_name | Biophysics |
| keywords[7].id | https://openalex.org/keywords/chemistry |
| keywords[7].score | 0.4626171886920929 |
| keywords[7].display_name | Chemistry |
| keywords[8].id | https://openalex.org/keywords/protein-structure |
| keywords[8].score | 0.4287511706352234 |
| keywords[8].display_name | Protein structure |
| keywords[9].id | https://openalex.org/keywords/biology |
| keywords[9].score | 0.35113656520843506 |
| keywords[9].display_name | Biology |
| keywords[10].id | https://openalex.org/keywords/biochemistry |
| keywords[10].score | 0.21067258715629578 |
| keywords[10].display_name | Biochemistry |
| language | en |
| locations[0].id | doi:10.1101/557496 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S4306402567 |
| locations[0].source.issn | |
| locations[0].source.type | repository |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | |
| locations[0].source.is_core | False |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| locations[0].source.host_organization | https://openalex.org/I2750212522 |
| locations[0].source.host_organization_name | Cold Spring Harbor Laboratory |
| locations[0].source.host_organization_lineage | https://openalex.org/I2750212522 |
| locations[0].license | cc-by-nc-nd |
| locations[0].pdf_url | https://www.biorxiv.org/content/biorxiv/early/2019/02/21/557496.full.pdf |
| locations[0].version | acceptedVersion |
| locations[0].raw_type | posted-content |
| locations[0].license_id | https://openalex.org/licenses/cc-by-nc-nd |
| locations[0].is_accepted | True |
| locations[0].is_published | False |
| locations[0].raw_source_name | |
| locations[0].landing_page_url | https://doi.org/10.1101/557496 |
| indexed_in | crossref |
| authorships[0].author.id | https://openalex.org/A5089012220 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-6637-3550 |
| authorships[0].author.display_name | Basavraj Khanppnavar |
| authorships[0].countries | IN |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I99364266 |
| authorships[0].affiliations[0].raw_affiliation_string | Academy of Scientific and Innovative Research (AcSIR) |
| authorships[0].affiliations[1].institution_ids | https://openalex.org/I160344538 |
| authorships[0].affiliations[1].raw_affiliation_string | Department of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), India |
| authorships[0].institutions[0].id | https://openalex.org/I99364266 |
| authorships[0].institutions[0].ror | https://ror.org/053rcsq61 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I99364266 |
| authorships[0].institutions[0].country_code | IN |
| authorships[0].institutions[0].display_name | Academy of Scientific and Innovative Research |
| authorships[0].institutions[1].id | https://openalex.org/I160344538 |
| authorships[0].institutions[1].ror | https://ror.org/01kh0x418 |
| authorships[0].institutions[1].type | facility |
| authorships[0].institutions[1].lineage | https://openalex.org/I160344538, https://openalex.org/I2799351866, https://openalex.org/I4210134808, https://openalex.org/I66760702 |
| authorships[0].institutions[1].country_code | IN |
| authorships[0].institutions[1].display_name | Indian Institute of Chemical Biology |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Basavraj Khanppnavar |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Academy of Scientific and Innovative Research (AcSIR), Department of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), India |
| authorships[1].author.id | https://openalex.org/A5055998046 |
| authorships[1].author.orcid | https://orcid.org/0000-0001-9153-6609 |
| authorships[1].author.display_name | Anupam Roy |
| authorships[1].countries | IN |
| authorships[1].affiliations[0].raw_affiliation_string | NMR research center, Indian Institute of Science (IISc), India |
| authorships[1].affiliations[1].institution_ids | https://openalex.org/I160344538 |
| authorships[1].affiliations[1].raw_affiliation_string | Department of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), India |
| authorships[1].institutions[0].id | https://openalex.org/I160344538 |
| authorships[1].institutions[0].ror | https://ror.org/01kh0x418 |
| authorships[1].institutions[0].type | facility |
| authorships[1].institutions[0].lineage | https://openalex.org/I160344538, https://openalex.org/I2799351866, https://openalex.org/I4210134808, https://openalex.org/I66760702 |
| authorships[1].institutions[0].country_code | IN |
| authorships[1].institutions[0].display_name | Indian Institute of Chemical Biology |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Anupam Roy |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Department of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), India, NMR research center, Indian Institute of Science (IISc), India |
| authorships[2].author.id | https://openalex.org/A5056825657 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-3220-8557 |
| authorships[2].author.display_name | Kousik Chandra |
| authorships[2].affiliations[0].raw_affiliation_string | NMR research center, Indian Institute of Science (IISc), India |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Kousik Chandra |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | NMR research center, Indian Institute of Science (IISc), India |
| authorships[3].author.id | https://openalex.org/A5080505874 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-8498-6502 |
| authorships[3].author.display_name | Nakul C. Maiti |
| authorships[3].countries | IN |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I99364266 |
| authorships[3].affiliations[0].raw_affiliation_string | Academy of Scientific and Innovative Research (AcSIR) |
| authorships[3].affiliations[1].institution_ids | https://openalex.org/I160344538 |
| authorships[3].affiliations[1].raw_affiliation_string | Department of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), India |
| authorships[3].institutions[0].id | https://openalex.org/I99364266 |
| authorships[3].institutions[0].ror | https://ror.org/053rcsq61 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I99364266 |
| authorships[3].institutions[0].country_code | IN |
| authorships[3].institutions[0].display_name | Academy of Scientific and Innovative Research |
| authorships[3].institutions[1].id | https://openalex.org/I160344538 |
| authorships[3].institutions[1].ror | https://ror.org/01kh0x418 |
| authorships[3].institutions[1].type | facility |
| authorships[3].institutions[1].lineage | https://openalex.org/I160344538, https://openalex.org/I2799351866, https://openalex.org/I4210134808, https://openalex.org/I66760702 |
| authorships[3].institutions[1].country_code | IN |
| authorships[3].institutions[1].display_name | Indian Institute of Chemical Biology |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Nakul Chandra Maiti |
| authorships[3].is_corresponding | True |
| authorships[3].raw_affiliation_strings | Academy of Scientific and Innovative Research (AcSIR), Department of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), India |
| authorships[4].author.id | https://openalex.org/A5076786243 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-3638-9383 |
| authorships[4].author.display_name | Saumen Datta |
| authorships[4].countries | IN |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I160344538 |
| authorships[4].affiliations[0].raw_affiliation_string | Department of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), India |
| authorships[4].affiliations[1].institution_ids | https://openalex.org/I99364266 |
| authorships[4].affiliations[1].raw_affiliation_string | Academy of Scientific and Innovative Research (AcSIR) |
| authorships[4].institutions[0].id | https://openalex.org/I99364266 |
| authorships[4].institutions[0].ror | https://ror.org/053rcsq61 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I99364266 |
| authorships[4].institutions[0].country_code | IN |
| authorships[4].institutions[0].display_name | Academy of Scientific and Innovative Research |
| authorships[4].institutions[1].id | https://openalex.org/I160344538 |
| authorships[4].institutions[1].ror | https://ror.org/01kh0x418 |
| authorships[4].institutions[1].type | facility |
| authorships[4].institutions[1].lineage | https://openalex.org/I160344538, https://openalex.org/I2799351866, https://openalex.org/I4210134808, https://openalex.org/I66760702 |
| authorships[4].institutions[1].country_code | IN |
| authorships[4].institutions[1].display_name | Indian Institute of Chemical Biology |
| authorships[4].author_position | last |
| authorships[4].raw_author_name | Saumen Datta |
| authorships[4].is_corresponding | True |
| authorships[4].raw_affiliation_strings | Academy of Scientific and Innovative Research (AcSIR), Department of Structural Biology and Bioinformatics, CSIR-Indian Institute of Chemical Biology (CSIR-IICB), India |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.biorxiv.org/content/biorxiv/early/2019/02/21/557496.full.pdf |
| open_access.oa_status | green |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Deciphering the structural intricacy in virulence effectors for proton-motive force mediated unfolding and type-III protein secretion |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10044 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.9980999827384949 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1312 |
| primary_topic.subfield.display_name | Molecular Biology |
| primary_topic.display_name | Protein Structure and Dynamics |
| related_works | https://openalex.org/W2470108822, https://openalex.org/W4224305457, https://openalex.org/W2116909324, https://openalex.org/W4244161128, https://openalex.org/W1549873021, https://openalex.org/W1577035833, https://openalex.org/W2120190359, https://openalex.org/W2115997854, https://openalex.org/W3012114985, https://openalex.org/W2160402508 |
| cited_by_count | 0 |
| locations_count | 1 |
| best_oa_location.id | doi:10.1101/557496 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4306402567 |
| best_oa_location.source.issn | |
| best_oa_location.source.type | repository |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | |
| best_oa_location.source.is_core | False |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| best_oa_location.source.host_organization | https://openalex.org/I2750212522 |
| best_oa_location.source.host_organization_name | Cold Spring Harbor Laboratory |
| best_oa_location.source.host_organization_lineage | https://openalex.org/I2750212522 |
| best_oa_location.license | cc-by-nc-nd |
| best_oa_location.pdf_url | https://www.biorxiv.org/content/biorxiv/early/2019/02/21/557496.full.pdf |
| best_oa_location.version | acceptedVersion |
| best_oa_location.raw_type | posted-content |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by-nc-nd |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | False |
| best_oa_location.raw_source_name | |
| best_oa_location.landing_page_url | https://doi.org/10.1101/557496 |
| primary_location.id | doi:10.1101/557496 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S4306402567 |
| primary_location.source.issn | |
| primary_location.source.type | repository |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | |
| primary_location.source.is_core | False |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| primary_location.source.host_organization | https://openalex.org/I2750212522 |
| primary_location.source.host_organization_name | Cold Spring Harbor Laboratory |
| primary_location.source.host_organization_lineage | https://openalex.org/I2750212522 |
| primary_location.license | cc-by-nc-nd |
| primary_location.pdf_url | https://www.biorxiv.org/content/biorxiv/early/2019/02/21/557496.full.pdf |
| primary_location.version | acceptedVersion |
| primary_location.raw_type | posted-content |
| primary_location.license_id | https://openalex.org/licenses/cc-by-nc-nd |
| primary_location.is_accepted | True |
| primary_location.is_published | False |
| primary_location.raw_source_name | |
| primary_location.landing_page_url | https://doi.org/10.1101/557496 |
| publication_date | 2019-02-21 |
| publication_year | 2019 |
| referenced_works | https://openalex.org/W2605765536, https://openalex.org/W2308145610, https://openalex.org/W2031923764, https://openalex.org/W2089227855, https://openalex.org/W2095952967, https://openalex.org/W2056525859, https://openalex.org/W2045673770, https://openalex.org/W2009035369, https://openalex.org/W2128882321, https://openalex.org/W2137121488, https://openalex.org/W2170516477, https://openalex.org/W2062892191, https://openalex.org/W2037300459, https://openalex.org/W2809878403, https://openalex.org/W2036977764, https://openalex.org/W2374112384, https://openalex.org/W2006943532, https://openalex.org/W2604527804, https://openalex.org/W2043981786, https://openalex.org/W1983277607, https://openalex.org/W2179902466, https://openalex.org/W2606365851, https://openalex.org/W2098483342, https://openalex.org/W2144670399, https://openalex.org/W1990299056, https://openalex.org/W1530729903, https://openalex.org/W2151143141, https://openalex.org/W1974253064, https://openalex.org/W1992045220, https://openalex.org/W1942728093, https://openalex.org/W2156408001, https://openalex.org/W2085142174, https://openalex.org/W2148494031, https://openalex.org/W2116929905, https://openalex.org/W2050425137, https://openalex.org/W2031016294, https://openalex.org/W2807273963, https://openalex.org/W2131754023, https://openalex.org/W1988123226, https://openalex.org/W2339205744, https://openalex.org/W2148659719, https://openalex.org/W1913602352, https://openalex.org/W2150860983, https://openalex.org/W2581718004, https://openalex.org/W2152384518, https://openalex.org/W2008946448, https://openalex.org/W2059816990, https://openalex.org/W1974819439, https://openalex.org/W1994840640, https://openalex.org/W1501347866, https://openalex.org/W2063937171, https://openalex.org/W2107904341, https://openalex.org/W2065023288, https://openalex.org/W2119693327, https://openalex.org/W2076742999, https://openalex.org/W2043308634, https://openalex.org/W2307152728, https://openalex.org/W2170323490, https://openalex.org/W2328101634, https://openalex.org/W2161199282, https://openalex.org/W2291836267, https://openalex.org/W2136070394, https://openalex.org/W2112837356, https://openalex.org/W2087928415, https://openalex.org/W2540069603, https://openalex.org/W2154501851, https://openalex.org/W2568226856, https://openalex.org/W2023490488, https://openalex.org/W1967055944, https://openalex.org/W2805756256 |
| referenced_works_count | 70 |
| abstract_inverted_index.In | 58 |
| abstract_inverted_index.an | 38, 63 |
| abstract_inverted_index.be | 153 |
| abstract_inverted_index.by | 83 |
| abstract_inverted_index.do | 43 |
| abstract_inverted_index.in | 53, 139, 182, 201, 215, 227, 232, 245 |
| abstract_inverted_index.is | 37, 117 |
| abstract_inverted_index.it | 102, 151 |
| abstract_inverted_index.of | 19, 34, 50, 66, 70, 95, 115, 127, 147, 164, 223, 236 |
| abstract_inverted_index.on | 220 |
| abstract_inverted_index.to | 11, 120, 159, 174 |
| abstract_inverted_index.we | 74 |
| abstract_inverted_index.371 | 148 |
| abstract_inverted_index.III | 7 |
| abstract_inverted_index.PMF | 231 |
| abstract_inverted_index.The | 178 |
| abstract_inverted_index.and | 47, 68, 93, 125, 135, 166, 196, 212, 230, 239, 247 |
| abstract_inverted_index.can | 87, 152 |
| abstract_inverted_index.due | 119 |
| abstract_inverted_index.for | 29, 186, 242 |
| abstract_inverted_index.its | 121 |
| abstract_inverted_index.the | 17, 25, 77, 106, 112, 140, 206, 233 |
| abstract_inverted_index.use | 5 |
| abstract_inverted_index.via | 31 |
| abstract_inverted_index.was | 103 |
| abstract_inverted_index.ExoY | 116 |
| abstract_inverted_index.From | 143 |
| abstract_inverted_index.IDPs | 165 |
| abstract_inverted_index.Many | 1 |
| abstract_inverted_index.T3SS | 35, 71, 149, 156, 228 |
| abstract_inverted_index.cost | 109 |
| abstract_inverted_index.from | 62, 131, 192 |
| abstract_inverted_index.have | 167 |
| abstract_inverted_index.host | 21 |
| abstract_inverted_index.into | 16 |
| abstract_inverted_index.pore | 33 |
| abstract_inverted_index.role | 222 |
| abstract_inverted_index.shed | 218 |
| abstract_inverted_index.show | 75 |
| abstract_inverted_index.that | 24, 76, 105, 155 |
| abstract_inverted_index.this | 59 |
| abstract_inverted_index.type | 6 |
| abstract_inverted_index.with | 111 |
| abstract_inverted_index.(PMF) | 86 |
| abstract_inverted_index.ExoY, | 73 |
| abstract_inverted_index.Given | 23 |
| abstract_inverted_index.class | 161 |
| abstract_inverted_index.cost. | 100 |
| abstract_inverted_index.force | 85 |
| abstract_inverted_index.found | 104 |
| abstract_inverted_index.light | 219 |
| abstract_inverted_index.lower | 107 |
| abstract_inverted_index.stems | 191 |
| abstract_inverted_index.their | 202 |
| abstract_inverted_index.toxic | 51 |
| abstract_inverted_index.water | 133 |
| abstract_inverted_index.(T3SS) | 10 |
| abstract_inverted_index.(∼pH | 80 |
| abstract_inverted_index.belong | 158 |
| abstract_inverted_index.buried | 132 |
| abstract_inverted_index.cells. | 22 |
| abstract_inverted_index.cores. | 142 |
| abstract_inverted_index.first, | 61 |
| abstract_inverted_index.folded | 123, 137, 141 |
| abstract_inverted_index.global | 113 |
| abstract_inverted_index.inject | 12 |
| abstract_inverted_index.mainly | 118 |
| abstract_inverted_index.mostly | 190 |
| abstract_inverted_index.remain | 55 |
| abstract_inverted_index.slower | 179 |
| abstract_inverted_index.study, | 60 |
| abstract_inverted_index.system | 9 |
| abstract_inverted_index.unfold | 46 |
| abstract_inverted_index.weakly | 122 |
| abstract_inverted_index.“How | 42 |
| abstract_inverted_index.Lastly, | 205 |
| abstract_inverted_index.balance | 244 |
| abstract_inverted_index.cytosol | 18 |
| abstract_inverted_index.density | 195 |
| abstract_inverted_index.evolved | 168, 208, 224 |
| abstract_inverted_index.folding | 67, 92, 180, 238 |
| abstract_inverted_index.largely | 56 |
| abstract_inverted_index.mediate | 175 |
| abstract_inverted_index.protein | 26 |
| abstract_inverted_index.reduced | 193 |
| abstract_inverted_index.secrete | 48 |
| abstract_inverted_index.similar | 169 |
| abstract_inverted_index.typical | 160 |
| abstract_inverted_index.without | 97 |
| abstract_inverted_index.5.8-6.0) | 81 |
| abstract_inverted_index.ABSTRACT | 0 |
| abstract_inverted_index.amortize | 89 |
| abstract_inverted_index.analysis | 65, 146 |
| abstract_inverted_index.bacteria | 4, 45 |
| abstract_inverted_index.directly | 15 |
| abstract_inverted_index.effector | 72, 183, 216, 237 |
| abstract_inverted_index.enhanced | 197 |
| abstract_inverted_index.entropic | 99 |
| abstract_inverted_index.function | 248 |
| abstract_inverted_index.geometry | 124 |
| abstract_inverted_index.gradient | 79 |
| abstract_inverted_index.hundreds | 49 |
| abstract_inverted_index.in-depth | 64 |
| abstract_inverted_index.kinetics | 181 |
| abstract_inverted_index.process, | 41 |
| abstract_inverted_index.proteins | 52, 184, 217 |
| abstract_inverted_index.residues | 214 |
| abstract_inverted_index.sequence | 203 |
| abstract_inverted_index.stemming | 130 |
| abstract_inverted_index.targeted | 20 |
| abstract_inverted_index.tertiary | 90 |
| abstract_inverted_index.unknown. | 57 |
| abstract_inverted_index.(disorder | 162 |
| abstract_inverted_index.abundance | 126 |
| abstract_inverted_index.chemistry | 226 |
| abstract_inverted_index.conserved | 170 |
| abstract_inverted_index.curtained | 154 |
| abstract_inverted_index.effectors | 14, 96, 157, 229 |
| abstract_inverted_index.efficient | 187 |
| abstract_inverted_index.energetic | 108 |
| abstract_inverted_index.essential | 241 |
| abstract_inverted_index.generated | 82 |
| abstract_inverted_index.globules) | 163 |
| abstract_inverted_index.in-silico | 144 |
| abstract_inverted_index.intrinsic | 171 |
| abstract_inverted_index.molecules | 134 |
| abstract_inverted_index.nano-size | 32 |
| abstract_inverted_index.repulsive | 199 |
| abstract_inverted_index.requisite | 28, 185 |
| abstract_inverted_index.secretion | 8, 30, 189, 246 |
| abstract_inverted_index.stability | 69, 94, 240 |
| abstract_inverted_index.unfolding | 27, 114 |
| abstract_inverted_index.virulence | 13 |
| abstract_inverted_index.archetypes | 173 |
| abstract_inverted_index.associated | 110 |
| abstract_inverted_index.effectors, | 150 |
| abstract_inverted_index.pathogenic | 3, 44 |
| abstract_inverted_index.positively | 207 |
| abstract_inverted_index.regulation | 235 |
| abstract_inverted_index.seconds” | 54 |
| abstract_inverted_index.structural | 91, 145, 172, 225 |
| abstract_inverted_index.trade-off. | 249 |
| abstract_inverted_index.unfolding. | 177 |
| abstract_inverted_index.Strikingly, | 101 |
| abstract_inverted_index.early-stage | 176 |
| abstract_inverted_index.gate-keeper | 213 |
| abstract_inverted_index.geometrical | 128 |
| abstract_inverted_index.hydrophobic | 194 |
| abstract_inverted_index.injectisome | 36 |
| abstract_inverted_index.landscapes. | 204 |
| abstract_inverted_index.maintaining | 243 |
| abstract_inverted_index.native-like | 136 |
| abstract_inverted_index.polar-polar | 198 |
| abstract_inverted_index.significant | 98 |
| abstract_inverted_index.stabilizing | 210 |
| abstract_inverted_index.unfavorable | 40 |
| abstract_inverted_index.frustrations | 129 |
| abstract_inverted_index.interactions | 200, 211 |
| abstract_inverted_index.T3SS-mediated | 188 |
| abstract_inverted_index.collaborative | 221 |
| abstract_inverted_index.energetically | 39 |
| abstract_inverted_index.gram-negative | 2 |
| abstract_inverted_index.intermediates | 138 |
| abstract_inverted_index.proton-motive | 84 |
| abstract_inverted_index.significantly | 88 |
| abstract_inverted_index.spatial-temporal | 234 |
| abstract_inverted_index.histidine-mediated | 209 |
| abstract_inverted_index.proton-concentration | 78 |
| cited_by_percentile_year | |
| corresponding_author_ids | https://openalex.org/A5080505874, https://openalex.org/A5076786243 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 5 |
| corresponding_institution_ids | https://openalex.org/I160344538, https://openalex.org/I99364266 |
| citation_normalized_percentile.value | 0.01414378 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |