Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-Down Proteomics Article Swipe
Che‐Fan Huang
,
Michael A. R. Hollas
,
Aniel Sánchez
,
Mrittika Bhattacharya
,
Giang Ho
,
Ambika Sundaresan
,
Michael A. Caldwell
,
Xiaoyan Zhao
,
Ryan W. Benz
,
Asim Siddiqui
,
Neil L. Kelleher
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1021/acs.jproteome.4c00621
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1021/acs.jproteome.4c00621
The dynamic range challenge for the detection of proteins and their proteoforms in human plasma has been well documented. Here, we use the nanoparticle protein corona approach to enrich low-abundance proteins selectively and reproducibly from human plasma and use top-down proteomics to quantify differential enrichment for the 2841 detected proteoforms from 114 proteins. Furthermore, nanoparticle enrichment allowed top-down detection of proteoforms between ∼1 μg/mL and ∼10 pg/mL in absolute abundance, providing up to a 105-fold increase in proteome depth over neat plasma in which only proteoforms from abundant proteins (>1 μg/mL) were detected. The ability to monitor medium and some low-abundant proteoforms through reproducible enrichment significantly extends the applicability of proteoform research by adding depth beyond albumin, immunoglobins, and apolipoproteins to uncover many involved in immunity and cell signaling. As proteoforms carry unique information content relative to peptides, this report opens the door to deeper proteoform sequencing in clinical proteomics of disease or aging cohorts.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1021/acs.jproteome.4c00621
- OA Status
- green
- Cited By
- 16
- References
- 51
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4402738677
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4402738677Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1021/acs.jproteome.4c00621Digital Object Identifier
- Title
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Deep Profiling of Plasma Proteoforms with Engineered Nanoparticles for Top-Down ProteomicsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-09-23Full publication date if available
- Authors
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Che‐Fan Huang, Michael A. R. Hollas, Aniel Sánchez, Mrittika Bhattacharya, Giang Ho, Ambika Sundaresan, Michael A. Caldwell, Xiaoyan Zhao, Ryan W. Benz, Asim Siddiqui, Neil L. KelleherList of authors in order
- Landing page
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https://doi.org/10.1021/acs.jproteome.4c00621Publisher landing page
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://pmc.ncbi.nlm.nih.gov/articles/PMC11789057/Direct OA link when available
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Profiling (computer programming), Proteomics, Nanoparticle, Nanotechnology, Plasma, Chemistry, Computational biology, Computer science, Materials science, Biology, Physics, Biochemistry, Gene, Quantum mechanics, Operating systemTop concepts (fields/topics) attached by OpenAlex
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16Total citation count in OpenAlex
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2025: 14, 2024: 2Per-year citation counts (last 5 years)
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51Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.10<sup>5</sup>-fold | 74 |
| cited_by_percentile_year.max | 100 |
| cited_by_percentile_year.min | 94 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 11 |
| citation_normalized_percentile.value | 0.96636235 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | True |