Design and characterisation of mutant and wild-type huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems Article Swipe
Rachel Harding
,
P. Loppnau
,
Suzanne Ackloo
,
Alexander Lemak
,
Ashley Hutchinson
,
Brittany Hunt
,
Alex S. Holehouse
,
Jolene Caifeng Ho
,
Lixin Fan
,
Leticia Toledo‐Sherman
,
Alma Seitova
,
C.H. Arrowsmith
·
YOU?
·
· 2018
· Open Access
·
· DOI: https://doi.org/10.5281/zenodo.2591677
YOU?
·
· 2018
· Open Access
·
· DOI: https://doi.org/10.5281/zenodo.2591677
ABSTRACT: The pathogenic Huntington’s disease (HD) mutation causes polyglutamine (polyQ) tract expansion of the 348 kDa HTT protein above a critical threshold of ~35 glutamines. HD mutation effect on HTT is poorly understood, partly due to difficulties in performing biochemical studies with this large protein. To facilitate such studies, we generated resources for HTT production in multiple eukaryotic expression systems, comprising constructs with polyQ lengths representing general population, HD patients, juvenile HD patients and the more extreme expansions used in some tissue and animal models. These reagents yield milligram quantities of pure HTT protein. We biophysically characterised HTT samples produced using this HD resource, gleaning insight into the nature of full-length HTT in its apo form and when bound to its binding partner HAP40. Work outlined in this manuscript and the tools generated, lay a foundation for further biochemical study of the HTT protein and its functional interactions with other biomolecules. NB: HTT_Manuscript_20190319.zip should contain all of the raw data used for this manuscript including raw images for the Western blots, missing in the previous upload.
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- Type
- article
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- en
- Landing Page
- https://zenodo.org/record/2591677
- OA Status
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- OpenAlex ID
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- DOI
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https://doi.org/10.5281/zenodo.2591677Digital Object Identifier
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Design and characterisation of mutant and wild-type huntingtin proteins produced from a toolkit of scalable eukaryotic expression systemsWork title
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articleOpenAlex work type
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enPrimary language
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2018Year of publication
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2018-12-10Full publication date if available
- Authors
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Rachel Harding, P. Loppnau, Suzanne Ackloo, Alexander Lemak, Ashley Hutchinson, Brittany Hunt, Alex S. Holehouse, Jolene Caifeng Ho, Lixin Fan, Leticia Toledo‐Sherman, Alma Seitova, C.H. ArrowsmithList of authors in order
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https://zenodo.org/record/2591677Publisher landing page
- Open access
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YesWhether a free full text is available
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greenOpen access status per OpenAlex
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https://zenodo.org/record/2591677Direct OA link when available
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Huntingtin, Mutant, Scalability, Computational biology, Biology, Wild type, Cell biology, Expression (computer science), Genetics, Computer science, Gene, Database, Programming languageTop concepts (fields/topics) attached by OpenAlex
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0Total citation count in OpenAlex
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