Root-knot nematodes (RKN) are microscopic plant parasites that cause significant economic damage to crops and vegetables. Accurate assessment of RKN populations is required for effective management of this disease. Several “You Only Look Once” (YOLO versions 2 to 7) architectures were investigated for the application of RKN enumeration in microscopic images. YOLOv5-608 model attained Precision score of 0.960, Recall of 0.951, F1-score of 0.990, mAP of 0.972 without mosaic augmentation. Using mosaic dataset, this w…