Detection and isolation of Duck Plague virus from field outbreaks in Assam, India Article Swipe
Samsun Neher
,
Nagendra Nath Barman
,
Durlav Prasad Bora
,
Dipak Deka
,
Shantanu Tamuly
,
Purabi Deka
,
Arpita Bharali
,
Sailendra Kumar Das
·
YOU?
·
· 2018
· Open Access
·
· DOI: https://doi.org/10.18805/ijar.b-3588
YOU?
·
· 2018
· Open Access
·
· DOI: https://doi.org/10.18805/ijar.b-3588
Duck plague is an acute, contagious and lethal disease of ducks caused by an enveloped DNA virus, belonging to Anatid Herpes Virus 1 under genus Mardivirus, subfamily Alphaherpesvirinae, family Herpesviridae. The disease is characterized by sudden death, high mortality, hemorrhages and necrosis in the internal organs. Duck farming plays an important role in the livelihood of the farmers of Assam. Duck Plague being the major killer disease among duck population of Assam, posing a threat to the growth of duck farming in the state. The present report investigates various outbreaks of Duck plague in Assam, detection and isolation of the virus. A total of 29 outbreaks suspected for Duck Plague were attended in different districts of Assam and 380 numbers of samples comprising of both clinical (n=107) as well as post mortem (n=273) were collected. Presence of DPV in samples was detected by Sandwich-ELISA (S-ELISA) and amplification of UL 44.5 as well as DNA polymerase gene of DPV by PCR. Out of 380 samples collected from 29 outbreaks, 213 (56.05%) and 299 (78.68%) samples were found positive for presence of DPV by S-ELISA and PCR, respectively. Pooled positive samples from various outbreaks were processed for isolation of duck plague virus in DEF primary cell culture. DPV field strain produced CPE comprising of rounding of cells, formation of syncytia, marked cytoplasmic granulations and intracellular vacuoles.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.18805/ijar.b-3588
- OA Status
- hybrid
- Cited By
- 7
- Related Works
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- OpenAlex ID
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All OpenAlex metadata
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https://openalex.org/W2884585667Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.18805/ijar.b-3588Digital Object Identifier
- Title
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Detection and isolation of Duck Plague virus from field outbreaks in Assam, IndiaWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2018Year of publication
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2018-06-21Full publication date if available
- Authors
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Samsun Neher, Nagendra Nath Barman, Durlav Prasad Bora, Dipak Deka, Shantanu Tamuly, Purabi Deka, Arpita Bharali, Sailendra Kumar DasList of authors in order
- Landing page
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https://doi.org/10.18805/ijar.b-3588Publisher landing page
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YesWhether a free full text is available
- OA status
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hybridOpen access status per OpenAlex
- OA URL
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https://doi.org/10.18805/ijar.b-3588Direct OA link when available
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Outbreak, Veterinary medicine, Virus, Virology, Biology, Plague (disease), Population, Polymerase chain reaction, Medicine, Pathology, Gene, Biochemistry, Environmental healthTop concepts (fields/topics) attached by OpenAlex
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7Total citation count in OpenAlex
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2024: 2, 2023: 1, 2021: 4Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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