Development & Validation of RP-HPLC Method for the Determination of Oseltamivir Phosphate API Article Swipe
Tabassum Bano
,
Rupesh Dudhe
,
Nitin Kumar
·
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.5281/zenodo.3970048
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.5281/zenodo.3970048
A simple and rapid liquid chromatographic assay for the evaluation of potentially counterfeit oseltamivir phosphate has been developed and assessed. The present study describes the development of a new, simple, reproducible RP-HPLC method for the quantitative determination of Oseltamivir phosphate (OSP). The proposed method utilizes C-18 column 100mm x 4.6mm at 25◦C with isocratic run using Methanol and Acetonitrile (50:50 v/v) at a flow rate of 1ml/min. and UV detection at 227 nm. The method was statistically validated for linearity, ruggedness, robustness, precision, and accuracy. The calibration curve was obtained in the concentration range of 5-35μg/ml with correlation coefficient 0.996. The robustness of the method has been studied by slightly varying the chromatographic conditions.
Related Topics
Concepts
Chromatography
Calibration curve
Linearity
Acetonitrile
High-performance liquid chromatography
Chemistry
Correlation coefficient
Methanol
Robustness (evolution)
Accuracy and precision
Analytical Chemistry (journal)
Detection limit
Mathematics
Statistics
Gene
Biochemistry
Quantum mechanics
Physics
Organic chemistry
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.5281/zenodo.3970048
- OA Status
- green
- Cited By
- 1
- References
- 2
- Related Works
- 20
- OpenAlex ID
- https://openalex.org/W2151577648
All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W2151577648Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.5281/zenodo.3970048Digital Object Identifier
- Title
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Development & Validation of RP-HPLC Method for the Determination of Oseltamivir Phosphate APIWork title
- Type
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articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
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2015Year of publication
- Publication date
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2015-07-11Full publication date if available
- Authors
-
Tabassum Bano, Rupesh Dudhe, Nitin KumarList of authors in order
- Landing page
-
https://doi.org/10.5281/zenodo.3970048Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
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greenOpen access status per OpenAlex
- OA URL
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https://doi.org/10.5281/zenodo.3970048Direct OA link when available
- Concepts
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Chromatography, Calibration curve, Linearity, Acetonitrile, High-performance liquid chromatography, Chemistry, Correlation coefficient, Methanol, Robustness (evolution), Accuracy and precision, Analytical Chemistry (journal), Detection limit, Mathematics, Statistics, Gene, Biochemistry, Quantum mechanics, Physics, Organic chemistryTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
1Total citation count in OpenAlex
- Citations by year (recent)
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2019: 1Per-year citation counts (last 5 years)
- References (count)
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2Number of works referenced by this work
- Related works (count)
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20Other works algorithmically related by OpenAlex
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| abstract_inverted_index.developed | 17 |
| abstract_inverted_index.isocratic | 53 |
| abstract_inverted_index.phosphate | 14, 39 |
| abstract_inverted_index.validated | 77 |
| abstract_inverted_index.5-35μg/ml | 95 |
| abstract_inverted_index.evaluation | 9 |
| abstract_inverted_index.linearity, | 79 |
| abstract_inverted_index.precision, | 82 |
| abstract_inverted_index.robustness | 101 |
| abstract_inverted_index.Oseltamivir | 38 |
| abstract_inverted_index.calibration | 86 |
| abstract_inverted_index.coefficient | 98 |
| abstract_inverted_index.conditions. | 113 |
| abstract_inverted_index.correlation | 97 |
| abstract_inverted_index.counterfeit | 12 |
| abstract_inverted_index.development | 25 |
| abstract_inverted_index.oseltamivir | 13 |
| abstract_inverted_index.potentially | 11 |
| abstract_inverted_index.robustness, | 81 |
| abstract_inverted_index.ruggedness, | 80 |
| abstract_inverted_index.Acetonitrile | 58 |
| abstract_inverted_index.quantitative | 35 |
| abstract_inverted_index.reproducible | 30 |
| abstract_inverted_index.concentration | 92 |
| abstract_inverted_index.determination | 36 |
| abstract_inverted_index.statistically | 76 |
| abstract_inverted_index.chromatographic | 5, 112 |
| cited_by_percentile_year.max | 94 |
| cited_by_percentile_year.min | 90 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 3 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/6 |
| sustainable_development_goals[0].score | 0.5699999928474426 |
| sustainable_development_goals[0].display_name | Clean water and sanitation |
| citation_normalized_percentile.value | 0.0932785 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |