Development and Clinical Validation of a Potential Penside Colorimetric Loop-Mediated Isothermal Amplification Assay of Porcine Circovirus Type 3 Article Swipe
Jie Zhang
,
Miaomiao Li
,
Yunwen Ou
,
Danian Chen
,
Yaozhong Ding
,
Weibing Zhang
,
Yanjun Li
,
Qian Hou
,
Xiaoyun Li
,
Luoyi Zhou
,
Katarzyna Podgórska
,
Zaberezhnyĭ Ad
,
Anna Szczotka-Bochniarz
,
Yongsheng Liu
,
Yang Wang
·
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2021.758064
YOU?
·
· 2022
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2021.758064
Porcine circovirus type 3 (PCV3), a novel circovirus, imposes great burdens on the global pig industry. The penside tests for detecting PCV3 are critical for assessing the epidemiological status and working out disease prevention and control programs due to the unavailability of a commercial vaccine. A one-step molecular assay based on visual loop-mediated isothermal amplification (vLAMP) was developed for simple and rapid detection of PCV3. We compared its sensitivity and specificity with TaqMan quantitative real-time polymerase chain reaction (qPCR) and applied the developed assay in the epidemiological study of ( n = 407) pooled swine sera collected from almost the entire mainland China during the years 2017–2018. We also explored the feasibility of the vLAMP assay for detecting raw samples without a prior DNA isolation step to expand its application capability. Results showed that the vLAMP assay could reliably detect the PCV3 cap gene with a detection limit of 10 DNA copies equal to that of the Taqman qPCR assay. In the epidemiological study, the PCV3 positive detection rate for 407 swine pooled sera detected by the vLAMP assay was 37.35% (152/407), whereas it was 39.01% (159/407) for Taqman qPCR. For the detection method without genome extraction, the results kept satisfactory specificity (100%) but displayed lower sensitivity (100% for CT < 32), indicating the direct detection is not sensitive enough to discriminate the samples with low viral loads. The one-step vLAMP is a convenient, rapid, and cost-effective diagnostic for penside detection and will enable the epidemiological surveillance of PCV3, which has widely spread in mainland China.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fmicb.2021.758064
- https://www.frontiersin.org/articles/10.3389/fmicb.2021.758064/pdf
- OA Status
- gold
- Cited By
- 2
- References
- 23
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4205491031
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4205491031Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3389/fmicb.2021.758064Digital Object Identifier
- Title
-
Development and Clinical Validation of a Potential Penside Colorimetric Loop-Mediated Isothermal Amplification Assay of Porcine Circovirus Type 3Work title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2022Year of publication
- Publication date
-
2022-01-12Full publication date if available
- Authors
-
Jie Zhang, Miaomiao Li, Yunwen Ou, Danian Chen, Yaozhong Ding, Weibing Zhang, Yanjun Li, Qian Hou, Xiaoyun Li, Luoyi Zhou, Katarzyna Podgórska, Zaberezhnyĭ Ad, Anna Szczotka-Bochniarz, Yongsheng Liu, Yang WangList of authors in order
- Landing page
-
https://doi.org/10.3389/fmicb.2021.758064Publisher landing page
- PDF URL
-
https://www.frontiersin.org/articles/10.3389/fmicb.2021.758064/pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.frontiersin.org/articles/10.3389/fmicb.2021.758064/pdfDirect OA link when available
- Concepts
-
Porcine circovirus, TaqMan, Loop-mediated isothermal amplification, Virology, Detection limit, Biology, Polymerase chain reaction, DNA extraction, Real-time polymerase chain reaction, Molecular biology, DNA, Chromatography, Virus, Chemistry, Gene, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
2Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2024: 1Per-year citation counts (last 5 years)
- References (count)
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23Number of works referenced by this work
- Related works (count)
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.We | 65, 107 |
| abstract_inverted_index.by | 175 |
| abstract_inverted_index.in | 84, 253 |
| abstract_inverted_index.is | 216, 231 |
| abstract_inverted_index.it | 183 |
| abstract_inverted_index.of | 41, 63, 88, 112, 148, 155, 247 |
| abstract_inverted_index.on | 11, 50 |
| abstract_inverted_index.to | 38, 126, 153, 220 |
| abstract_inverted_index.407 | 170 |
| abstract_inverted_index.DNA | 123, 150 |
| abstract_inverted_index.For | 190 |
| abstract_inverted_index.The | 16, 228 |
| abstract_inverted_index.and | 29, 34, 60, 69, 79, 235, 241 |
| abstract_inverted_index.are | 22 |
| abstract_inverted_index.but | 203 |
| abstract_inverted_index.cap | 142 |
| abstract_inverted_index.due | 37 |
| abstract_inverted_index.for | 19, 24, 58, 116, 169, 187, 208, 238 |
| abstract_inverted_index.has | 250 |
| abstract_inverted_index.its | 67, 128 |
| abstract_inverted_index.low | 225 |
| abstract_inverted_index.not | 217 |
| abstract_inverted_index.out | 31 |
| abstract_inverted_index.pig | 14 |
| abstract_inverted_index.raw | 118 |
| abstract_inverted_index.the | 12, 26, 39, 81, 85, 99, 104, 110, 113, 134, 140, 156, 161, 164, 176, 191, 197, 213, 222, 244 |
| abstract_inverted_index.was | 56, 179, 184 |
| abstract_inverted_index.32), | 211 |
| abstract_inverted_index.407) | 92 |
| abstract_inverted_index.PCV3 | 21, 141, 165 |
| abstract_inverted_index.also | 108 |
| abstract_inverted_index.from | 97 |
| abstract_inverted_index.gene | 143 |
| abstract_inverted_index.kept | 199 |
| abstract_inverted_index.qPCR | 158 |
| abstract_inverted_index.rate | 168 |
| abstract_inverted_index.sera | 95, 173 |
| abstract_inverted_index.step | 125 |
| abstract_inverted_index.that | 133, 154 |
| abstract_inverted_index.type | 2 |
| abstract_inverted_index.will | 242 |
| abstract_inverted_index.with | 71, 144, 224 |
| abstract_inverted_index.(100% | 207 |
| abstract_inverted_index.China | 102 |
| abstract_inverted_index.PCV3, | 248 |
| abstract_inverted_index.PCV3. | 64 |
| abstract_inverted_index.assay | 48, 83, 115, 136, 178 |
| abstract_inverted_index.based | 49 |
| abstract_inverted_index.chain | 76 |
| abstract_inverted_index.could | 137 |
| abstract_inverted_index.equal | 152 |
| abstract_inverted_index.great | 9 |
| abstract_inverted_index.limit | 147 |
| abstract_inverted_index.lower | 205 |
| abstract_inverted_index.novel | 6 |
| abstract_inverted_index.prior | 122 |
| abstract_inverted_index.qPCR. | 189 |
| abstract_inverted_index.rapid | 61 |
| abstract_inverted_index.study | 87 |
| abstract_inverted_index.swine | 94, 171 |
| abstract_inverted_index.tests | 18 |
| abstract_inverted_index.vLAMP | 114, 135, 177, 230 |
| abstract_inverted_index.viral | 226 |
| abstract_inverted_index.which | 249 |
| abstract_inverted_index.years | 105 |
| abstract_inverted_index.(100%) | 202 |
| abstract_inverted_index.(qPCR) | 78 |
| abstract_inverted_index.37.35% | 180 |
| abstract_inverted_index.39.01% | 185 |
| abstract_inverted_index.China. | 255 |
| abstract_inverted_index.TaqMan | 72 |
| abstract_inverted_index.Taqman | 157, 188 |
| abstract_inverted_index.almost | 98 |
| abstract_inverted_index.assay. | 159 |
| abstract_inverted_index.copies | 151 |
| abstract_inverted_index.detect | 139 |
| abstract_inverted_index.direct | 214 |
| abstract_inverted_index.during | 103 |
| abstract_inverted_index.enable | 243 |
| abstract_inverted_index.enough | 219 |
| abstract_inverted_index.entire | 100 |
| abstract_inverted_index.expand | 127 |
| abstract_inverted_index.genome | 195 |
| abstract_inverted_index.global | 13 |
| abstract_inverted_index.loads. | 227 |
| abstract_inverted_index.method | 193 |
| abstract_inverted_index.pooled | 93, 172 |
| abstract_inverted_index.rapid, | 234 |
| abstract_inverted_index.showed | 132 |
| abstract_inverted_index.simple | 59 |
| abstract_inverted_index.spread | 252 |
| abstract_inverted_index.status | 28 |
| abstract_inverted_index.study, | 163 |
| abstract_inverted_index.visual | 51 |
| abstract_inverted_index.widely | 251 |
| abstract_inverted_index.(PCV3), | 4 |
| abstract_inverted_index.(vLAMP) | 55 |
| abstract_inverted_index.Porcine | 0 |
| abstract_inverted_index.Results | 131 |
| abstract_inverted_index.applied | 80 |
| abstract_inverted_index.burdens | 10 |
| abstract_inverted_index.control | 35 |
| abstract_inverted_index.disease | 32 |
| abstract_inverted_index.imposes | 8 |
| abstract_inverted_index.penside | 17, 239 |
| abstract_inverted_index.results | 198 |
| abstract_inverted_index.samples | 119, 223 |
| abstract_inverted_index.whereas | 182 |
| abstract_inverted_index.without | 120, 194 |
| abstract_inverted_index.working | 30 |
| abstract_inverted_index.&lt; | 210 |
| abstract_inverted_index.compared | 66 |
| abstract_inverted_index.critical | 23 |
| abstract_inverted_index.detected | 174 |
| abstract_inverted_index.explored | 109 |
| abstract_inverted_index.mainland | 101, 254 |
| abstract_inverted_index.one-step | 46, 229 |
| abstract_inverted_index.positive | 166 |
| abstract_inverted_index.programs | 36 |
| abstract_inverted_index.reaction | 77 |
| abstract_inverted_index.reliably | 138 |
| abstract_inverted_index.vaccine. | 44 |
| abstract_inverted_index.(159/407) | 186 |
| abstract_inverted_index.assessing | 25 |
| abstract_inverted_index.collected | 96 |
| abstract_inverted_index.detecting | 20, 117 |
| abstract_inverted_index.detection | 62, 146, 167, 192, 215, 240 |
| abstract_inverted_index.developed | 57, 82 |
| abstract_inverted_index.displayed | 204 |
| abstract_inverted_index.industry. | 15 |
| abstract_inverted_index.isolation | 124 |
| abstract_inverted_index.molecular | 47 |
| abstract_inverted_index.real-time | 74 |
| abstract_inverted_index.sensitive | 218 |
| abstract_inverted_index.(152/407), | 181 |
| abstract_inverted_index.circovirus | 1 |
| abstract_inverted_index.commercial | 43 |
| abstract_inverted_index.diagnostic | 237 |
| abstract_inverted_index.indicating | 212 |
| abstract_inverted_index.isothermal | 53 |
| abstract_inverted_index.polymerase | 75 |
| abstract_inverted_index.prevention | 33 |
| abstract_inverted_index.application | 129 |
| abstract_inverted_index.capability. | 130 |
| abstract_inverted_index.circovirus, | 7 |
| abstract_inverted_index.convenient, | 233 |
| abstract_inverted_index.extraction, | 196 |
| abstract_inverted_index.feasibility | 111 |
| abstract_inverted_index.sensitivity | 68, 206 |
| abstract_inverted_index.specificity | 70, 201 |
| abstract_inverted_index.2017–2018. | 106 |
| abstract_inverted_index.discriminate | 221 |
| abstract_inverted_index.quantitative | 73 |
| abstract_inverted_index.satisfactory | 200 |
| abstract_inverted_index.surveillance | 246 |
| abstract_inverted_index.amplification | 54 |
| abstract_inverted_index.loop-mediated | 52 |
| abstract_inverted_index.cost-effective | 236 |
| abstract_inverted_index.unavailability | 40 |
| abstract_inverted_index.epidemiological | 27, 86, 162, 245 |
| cited_by_percentile_year.max | 95 |
| cited_by_percentile_year.min | 90 |
| corresponding_author_ids | https://openalex.org/A5006213514, https://openalex.org/A5037903777, https://openalex.org/A5103030951 |
| countries_distinct_count | 3 |
| institutions_distinct_count | 15 |
| corresponding_institution_ids | https://openalex.org/I4210090981, https://openalex.org/I4210120736, https://openalex.org/I4210138501, https://openalex.org/I48381559 |
| citation_normalized_percentile.value | 0.49170521 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |