Development and Inter-Laboratory Validation of Diagnostics Panel for Detection of Biothreat Bacteria Based on MOL-PCR Assay Article Swipe
Pavlína Jelínková
,
Jakub Hrdý
,
Jiřina Marková
,
Jiří Dresler
,
Petr Pajer
,
Oto Pavliš
,
Pavel Branich
,
Gabriela Bořilová
,
Marketa Reichelova
,
Vladimír Babák
,
Nikol Reslová
,
Petr Králík
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.3390/microorganisms9010038
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.3390/microorganisms9010038
Early detection of biohazardous bacteria that can be misused as biological weapons is one of the most important measures to prevent the spread and outbreak of biological warfare. For this reason, many instrument platforms need to be introduced into operation in the field of biological warfare detection. Therefore the purpose of this study is to establish a new detection panel for biothreat bacteria (Bacillus anthracis, Yersinia pestis, Francisella tularensis, and Brucella spp.) and confirm it by collaborative validation by using a multiplex oligonucleotide ligation followed by polymerase chain reaction and hybridization to microspheres by MagPix detection platform (MOL-PCR). Appropriate specific sequences in bacterial DNA were selected and tested to assemble the detection panel, and MOLigo probes (short specific oligonucleotides) were designed to show no cross-reactivity when tested between bacteria and to decrease the background signal measurement on the MagPix platform. During testing, sensitivity was assessed for all target bacteria using serially diluted DNA and was determined to be at least 0.5 ng/µL. For use as a diagnostic kit and easier handling, the storage stability of ligation premixes (MOLigo probe mixes) was tested. This highly multiplex method can be used for rapid screening to prevent outbreaks arising from the use of bacterial strains for bioterrorism, because time of analysis take under 4 h.
Related Topics
Concepts
Francisella tularensis
Bacillus anthracis
Multiplex
Bacteria
Microbiology
Polymerase chain reaction
Biology
Oligonucleotide
Biological warfare
Yersinia pestis
Multiplex polymerase chain reaction
Virology
DNA
Gene
Toxicology
Bioinformatics
Virulence
Genetics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/microorganisms9010038
- https://www.mdpi.com/2076-2607/9/1/38/pdf?version=1611137484
- OA Status
- gold
- Cited By
- 2
- References
- 52
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3114928612
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W3114928612Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3390/microorganisms9010038Digital Object Identifier
- Title
-
Development and Inter-Laboratory Validation of Diagnostics Panel for Detection of Biothreat Bacteria Based on MOL-PCR AssayWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-12-24Full publication date if available
- Authors
-
Pavlína Jelínková, Jakub Hrdý, Jiřina Marková, Jiří Dresler, Petr Pajer, Oto Pavliš, Pavel Branich, Gabriela Bořilová, Marketa Reichelova, Vladimír Babák, Nikol Reslová, Petr KrálíkList of authors in order
- Landing page
-
https://doi.org/10.3390/microorganisms9010038Publisher landing page
- PDF URL
-
https://www.mdpi.com/2076-2607/9/1/38/pdf?version=1611137484Direct link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.mdpi.com/2076-2607/9/1/38/pdf?version=1611137484Direct OA link when available
- Concepts
-
Francisella tularensis, Bacillus anthracis, Multiplex, Bacteria, Microbiology, Polymerase chain reaction, Biology, Oligonucleotide, Biological warfare, Yersinia pestis, Multiplex polymerase chain reaction, Virology, DNA, Gene, Toxicology, Bioinformatics, Virulence, GeneticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
2Total citation count in OpenAlex
- Citations by year (recent)
-
2022: 2Per-year citation counts (last 5 years)
- References (count)
-
52Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.(MOLigo | 177 |
| abstract_inverted_index.arising | 195 |
| abstract_inverted_index.because | 204 |
| abstract_inverted_index.between | 127 |
| abstract_inverted_index.confirm | 73 |
| abstract_inverted_index.diluted | 151 |
| abstract_inverted_index.misused | 8 |
| abstract_inverted_index.ng/µL. | 161 |
| abstract_inverted_index.pestis, | 66 |
| abstract_inverted_index.prevent | 20, 193 |
| abstract_inverted_index.purpose | 49 |
| abstract_inverted_index.reason, | 30 |
| abstract_inverted_index.storage | 172 |
| abstract_inverted_index.strains | 201 |
| abstract_inverted_index.tested. | 181 |
| abstract_inverted_index.warfare | 45 |
| abstract_inverted_index.weapons | 11 |
| abstract_inverted_index.Brucella | 70 |
| abstract_inverted_index.Yersinia | 65 |
| abstract_inverted_index.analysis | 207 |
| abstract_inverted_index.assemble | 109 |
| abstract_inverted_index.assessed | 144 |
| abstract_inverted_index.bacteria | 4, 62, 128, 148 |
| abstract_inverted_index.decrease | 131 |
| abstract_inverted_index.designed | 120 |
| abstract_inverted_index.followed | 84 |
| abstract_inverted_index.ligation | 83, 175 |
| abstract_inverted_index.measures | 18 |
| abstract_inverted_index.outbreak | 24 |
| abstract_inverted_index.platform | 96 |
| abstract_inverted_index.premixes | 176 |
| abstract_inverted_index.reaction | 88 |
| abstract_inverted_index.selected | 105 |
| abstract_inverted_index.serially | 150 |
| abstract_inverted_index.specific | 99, 117 |
| abstract_inverted_index.testing, | 141 |
| abstract_inverted_index.warfare. | 27 |
| abstract_inverted_index.(Bacillus | 63 |
| abstract_inverted_index.Therefore | 47 |
| abstract_inverted_index.bacterial | 102, 200 |
| abstract_inverted_index.biothreat | 61 |
| abstract_inverted_index.detection | 1, 58, 95, 111 |
| abstract_inverted_index.establish | 55 |
| abstract_inverted_index.handling, | 170 |
| abstract_inverted_index.important | 17 |
| abstract_inverted_index.multiplex | 81, 184 |
| abstract_inverted_index.operation | 39 |
| abstract_inverted_index.outbreaks | 194 |
| abstract_inverted_index.platform. | 139 |
| abstract_inverted_index.platforms | 33 |
| abstract_inverted_index.screening | 191 |
| abstract_inverted_index.sequences | 100 |
| abstract_inverted_index.stability | 173 |
| abstract_inverted_index.(MOL-PCR). | 97 |
| abstract_inverted_index.anthracis, | 64 |
| abstract_inverted_index.background | 133 |
| abstract_inverted_index.biological | 10, 26, 44 |
| abstract_inverted_index.detection. | 46 |
| abstract_inverted_index.determined | 155 |
| abstract_inverted_index.diagnostic | 166 |
| abstract_inverted_index.instrument | 32 |
| abstract_inverted_index.introduced | 37 |
| abstract_inverted_index.polymerase | 86 |
| abstract_inverted_index.validation | 77 |
| abstract_inverted_index.Appropriate | 98 |
| abstract_inverted_index.Francisella | 67 |
| abstract_inverted_index.measurement | 135 |
| abstract_inverted_index.sensitivity | 142 |
| abstract_inverted_index.tularensis, | 68 |
| abstract_inverted_index.biohazardous | 3 |
| abstract_inverted_index.microspheres | 92 |
| abstract_inverted_index.bioterrorism, | 203 |
| abstract_inverted_index.collaborative | 76 |
| abstract_inverted_index.hybridization | 90 |
| abstract_inverted_index.oligonucleotide | 82 |
| abstract_inverted_index.cross-reactivity | 124 |
| abstract_inverted_index.oligonucleotides) | 118 |
| cited_by_percentile_year.max | 96 |
| cited_by_percentile_year.min | 94 |
| corresponding_author_ids | https://openalex.org/A5022850112 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 12 |
| corresponding_institution_ids | https://openalex.org/I4210118976 |
| citation_normalized_percentile.value | 0.52209024 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |