Development and Validation of a HS-SPME-GC-SIM-MS Multi-Method Targeting Hop-Derived Esters in Beer Article Swipe
Christian Schubert
,
Sarah Thörner
,
Laura Knoke
,
Nils Rettberg
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1080/03610470.2021.1994814
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1080/03610470.2021.1994814
Hop-derived esters contribute to beer flavor and changes in the ester spectrum are believed to be an important driver in the flavor–instability of hoppy beers. To date, there exists no published method that enables reliable quantification of hop-derived esters in beer. As the availability of such a method is vital to enlarge the understanding of beer flavor stability, the current article is concerned with the development, validation, and application of a headspace solid phase microextraction gas chromatography selected ion monitoring mass spectrometry (HS-SPME-GC-SIM-MS) based methodology for quantification of 16 hop-derived esters in beer. The validation data shows that choosing suitable HS-SPME-GC-SIM-MS conditions enabled reliable quantification of esters across a range of 1–200 µg/L, with calculated limits of quantification being well below 1 µg/L. Spiking experiments using terpenes and terpenoids evidenced method robustness, most importantly when two commercially available stable isotope labeled internal standards (d6-geranyl acetate and 13C-methyl octanoate) were used. Application to beer samples indicated that beers brewed with different qualities/quantities of hops and differing in freshness could be well differentiated by HS-SPME-GC-SIM-MS analysis of their ester profile.Supplemental data for this article is available online at at https://doi.org/10.1080/03610470.2021.1994814 .
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Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1080/03610470.2021.1994814
- https://www.tandfonline.com/doi/pdf/10.1080/03610470.2021.1994814?needAccess=true
- OA Status
- bronze
- Cited By
- 7
- References
- 21
- Related Works
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- OpenAlex ID
- https://openalex.org/W4200572676
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4200572676Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1080/03610470.2021.1994814Digital Object Identifier
- Title
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Development and Validation of a HS-SPME-GC-SIM-MS Multi-Method Targeting Hop-Derived Esters in BeerWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2021Year of publication
- Publication date
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2021-12-16Full publication date if available
- Authors
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Christian Schubert, Sarah Thörner, Laura Knoke, Nils RettbergList of authors in order
- Landing page
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https://doi.org/10.1080/03610470.2021.1994814Publisher landing page
- PDF URL
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https://www.tandfonline.com/doi/pdf/10.1080/03610470.2021.1994814?needAccess=trueDirect link to full text PDF
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YesWhether a free full text is available
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bronzeOpen access status per OpenAlex
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https://www.tandfonline.com/doi/pdf/10.1080/03610470.2021.1994814?needAccess=trueDirect OA link when available
- Concepts
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Chemistry, Chromatography, Flavor, Solid-phase microextraction, Gas chromatography–mass spectrometry, Hop (telecommunications), Mass spectrometry, Gas chromatography, Food science, Computer science, Computer networkTop concepts (fields/topics) attached by OpenAlex
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7Total citation count in OpenAlex
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2025: 2, 2024: 1, 2023: 2, 2022: 2Per-year citation counts (last 5 years)
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21Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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| abstract_inverted_index.µg/L. | 122 |
| abstract_inverted_index.1–200 | 111 |
| abstract_inverted_index.Spiking | 123 |
| abstract_inverted_index.acetate | 144 |
| abstract_inverted_index.article | 60, 181 |
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| abstract_inverted_index.octanoate) | 147 |
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| abstract_inverted_index.terpenoids | 128 |
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| abstract_inverted_index.(d6-geranyl | 143 |
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| abstract_inverted_index.https://doi.org/10.1080/03610470.2021.1994814 | 187 |
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| corresponding_author_ids | https://openalex.org/A5074223336 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 4 |
| corresponding_institution_ids | https://openalex.org/I4210153617 |
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| citation_normalized_percentile.is_in_top_10_percent | False |