Development and Validation of the Stability of p-SCN-Bn-Df via the Reversed-Phase Chromatography Method: Practical Experiences Article Swipe
Anjli Shrivastav
,
Mohd. Faheem
,
Vaibhav Pandey
,
Manish Dixit
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.3390/ecsoc-28-20175
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.3390/ecsoc-28-20175
The DFO, a special hexadentate chelator with three hydroxamate moieties, is a bifunctional 1-(4-isothiocyanatophenyl)-3-[6,17-dihydroxy-7,10,18,21-tetraoxo-27-(N-acetylhydroxylamino)- 6,11,17, 22- tetraazaheptaeicosine] thiourea (p-SCN-Bn-Df), a significant next-generation ligand. The presence of the thiocyanate (-SCN) group makes it capable of hydrolysis and the protonation process. In this study aims to optimize the HPLC protocol for 1-(4-isothiocyanatophenyl)-3-[6,17-dihydroxy-7,10,18,21-tetraoxo-27-(n-acetylhydroxylamino)-6,11,17,22-tetraazaheptaeicosine] thiourea (p-SCN-Bn-Df) via the Reversed-Phase Chromatography (RP-HPLC) method. A variety of mobile phases were tested in various ratios of solvent constituents such as methanol/water, acetonitrile/water, and phosphate buffer along with at variable pH concentrations. However, when employing a mobile phase consisting of water to acetonitrile containing 0.1% TFA (05:95, v/v) in an isocratic manner, satisfactory separation and symmetric peaks were observed. This method utilized an Eclipsed C-18 column (5 μm, 4.6 × 250 mm) column with a flow rate of 0.5 mL/min. The maximum absorption of p-SCN-Bn-Dfat 254 nm wavelength was selected as the detection wavelength. The Retention time (tR) of p-SCN-Bn-Df was found at 5.205 min. The ICH guideline was used to evaluate the linearity, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ), specificity, and system appropriateness criteria to validate the optimized chromatographic and spectrophotometric procedures. For accurate compound separation in pharmaceutical and environmental analyses, this phase is adaptable and often used. This study is useful for the evaluation of p-SCN-Bn-Df QC parameters and chelation rates with different radioisotopes e.g., Zirconuim-89 (Zr-89).
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- en
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- OA Status
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- OpenAlex ID
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https://openalex.org/W4405476250Canonical identifier for this work in OpenAlex
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https://doi.org/10.3390/ecsoc-28-20175Digital Object Identifier
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Development and Validation of the Stability of p-SCN-Bn-Df via the Reversed-Phase Chromatography Method: Practical ExperiencesWork title
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articleOpenAlex work type
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enPrimary language
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2024Year of publication
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2024-11-14Full publication date if available
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Anjli Shrivastav, Mohd. Faheem, Vaibhav Pandey, Manish DixitList of authors in order
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https://doi.org/10.3390/ecsoc-28-20175Publisher landing page
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goldOpen access status per OpenAlex
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| abstract_inverted_index.accuracy, | 166 |
| abstract_inverted_index.adaptable | 201 |
| abstract_inverted_index.analyses, | 197 |
| abstract_inverted_index.chelation | 217 |
| abstract_inverted_index.detection | 144, 170 |
| abstract_inverted_index.different | 220 |
| abstract_inverted_index.employing | 86 |
| abstract_inverted_index.guideline | 159 |
| abstract_inverted_index.isocratic | 102 |
| abstract_inverted_index.moieties, | 9 |
| abstract_inverted_index.observed. | 110 |
| abstract_inverted_index.optimized | 184 |
| abstract_inverted_index.phosphate | 76 |
| abstract_inverted_index.symmetric | 107 |
| abstract_inverted_index.absorption | 134 |
| abstract_inverted_index.consisting | 90 |
| abstract_inverted_index.containing | 95 |
| abstract_inverted_index.evaluation | 211 |
| abstract_inverted_index.hydrolysis | 34 |
| abstract_inverted_index.linearity, | 165 |
| abstract_inverted_index.parameters | 215 |
| abstract_inverted_index.precision, | 167 |
| abstract_inverted_index.separation | 105, 192 |
| abstract_inverted_index.wavelength | 139 |
| abstract_inverted_index.hexadentate | 4 |
| abstract_inverted_index.hydroxamate | 8 |
| abstract_inverted_index.p-SCN-Bn-Df | 151, 213 |
| abstract_inverted_index.procedures. | 188 |
| abstract_inverted_index.protonation | 37 |
| abstract_inverted_index.significant | 20 |
| abstract_inverted_index.thiocyanate | 27 |
| abstract_inverted_index.wavelength. | 145 |
| abstract_inverted_index.Zirconuim-89 | 223 |
| abstract_inverted_index.acetonitrile | 94 |
| abstract_inverted_index.bifunctional | 12 |
| abstract_inverted_index.constituents | 70 |
| abstract_inverted_index.quantitation | 174 |
| abstract_inverted_index.satisfactory | 104 |
| abstract_inverted_index.specificity, | 176 |
| abstract_inverted_index.(p-SCN-Bn-Df) | 51 |
| abstract_inverted_index.environmental | 196 |
| abstract_inverted_index.p-SCN-Bn-Dfat | 136 |
| abstract_inverted_index.radioisotopes | 221 |
| abstract_inverted_index.(p-SCN-Bn-Df), | 18 |
| abstract_inverted_index.Chromatography | 55 |
| abstract_inverted_index.Reversed-Phase | 54 |
| abstract_inverted_index.pharmaceutical | 194 |
| abstract_inverted_index.(t<sub>R</sub>) | 149 |
| abstract_inverted_index.appropriateness | 179 |
| abstract_inverted_index.chromatographic | 185 |
| abstract_inverted_index.concentrations. | 83 |
| abstract_inverted_index.methanol/water, | 73 |
| abstract_inverted_index.next-generation | 21 |
| abstract_inverted_index.<i>v</i>/<i>v</i>) | 99 |
| abstract_inverted_index.spectrophotometric | 187 |
| abstract_inverted_index.acetonitrile/water, | 74 |
| abstract_inverted_index.tetraazaheptaeicosine] | 16 |
| abstract_inverted_index.1-(4-isothiocyanatophenyl)-3-[6,17-dihydroxy-7,10,18,21-tetraoxo-27-(N-acetylhydroxylamino)- | 13 |
| abstract_inverted_index.1-(4-isothiocyanatophenyl)-3-[6,17-dihydroxy-7,10,18,21-tetraoxo-27-(<i>n</i>-acetylhydroxylamino)-6,11,17,22-tetraazaheptaeicosine] | 49 |
| cited_by_percentile_year | |
| countries_distinct_count | 1 |
| institutions_distinct_count | 4 |
| citation_normalized_percentile.value | 0.4410585 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |