Development of a lambda Red based system for gene deletion in Chlamydia Article Swipe
Yibing Wang
,
Robert J. Suchland
,
Amy Hua
,
Steven Carrell
,
Daniel D. Rockey
,
Kevin Hybiske
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0311630
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1371/journal.pone.0311630
The ability to efficiently target loci in the Chlamydia trachomatis genome for deletion remains a desirable goal in the field and new strategies need to be developed and refined. Here we describe the development and application of a lambda red recombineering system for Chlamydia . Using a non-replicative plasmid encoding key lambda Red components and targeting sequences, we demonstrate the efficient deletion of numerous gene targets in the model chlamydial strains C . trachomatis L2/434 and C . muridarum . For initial development of the system, we targeted the incA gene of C . trachomatis for deletion. Deletion mutants containing a chloramphenicol resistance marker were recovered within 3 rounds of selection, or 2 rounds of passaging, in McCoy cells and the resulting clones (CTΔ incA ) were verified by PCR-based genotyping and whole genome sequencing. Phenotypic assessment of host cells infected with CTΔ incA was performed by immunofluorescence microscopy and confirmed the lack of IncA expression and the uniform presence of nonfusogenic vacuoles (inclusions) across CTΔ incA -infected monolayers. To explore the utility of this system, we deleted 5 additional candidate virulence factors in C . trachomatis and C . muridarum , including deletions of single and multiple genes. We expect lambda Red recombineering to offer a powerful new strategy for making gene deletion and/or replacement mutants in Chlamydia .
Related Topics
Concepts
Recombineering
Biology
Chlamydia trachomatis
Gene
Genome
Genetics
Plasmid
Computational biology
Virology
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1371/journal.pone.0311630
- OA Status
- gold
- Cited By
- 3
- References
- 40
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4404376695
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4404376695Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1371/journal.pone.0311630Digital Object Identifier
- Title
-
Development of a lambda Red based system for gene deletion in ChlamydiaWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2024Year of publication
- Publication date
-
2024-11-14Full publication date if available
- Authors
-
Yibing Wang, Robert J. Suchland, Amy Hua, Steven Carrell, Daniel D. Rockey, Kevin HybiskeList of authors in order
- Landing page
-
https://doi.org/10.1371/journal.pone.0311630Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.1371/journal.pone.0311630Direct OA link when available
- Concepts
-
Recombineering, Biology, Chlamydia trachomatis, Gene, Genome, Genetics, Plasmid, Computational biology, VirologyTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
3Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 3Per-year citation counts (last 5 years)
- References (count)
-
40Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4404376695 |
|---|---|
| doi | https://doi.org/10.1371/journal.pone.0311630 |
| ids.doi | https://doi.org/10.1371/journal.pone.0311630 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/39541273 |
| ids.openalex | https://openalex.org/W4404376695 |
| fwci | 2.14497238 |
| mesh[0].qualifier_ui | |
| mesh[0].descriptor_ui | D017353 |
| mesh[0].is_major_topic | True |
| mesh[0].qualifier_name | |
| mesh[0].descriptor_name | Gene Deletion |
| mesh[1].qualifier_ui | Q000235 |
| mesh[1].descriptor_ui | D002692 |
| mesh[1].is_major_topic | True |
| mesh[1].qualifier_name | genetics |
| mesh[1].descriptor_name | Chlamydia trachomatis |
| mesh[2].qualifier_ui | Q000235 |
| mesh[2].descriptor_ui | D010582 |
| mesh[2].is_major_topic | True |
| mesh[2].qualifier_name | genetics |
| mesh[2].descriptor_name | Bacteriophage lambda |
| mesh[3].qualifier_ui | |
| mesh[3].descriptor_ui | D006801 |
| mesh[3].is_major_topic | False |
| mesh[3].qualifier_name | |
| mesh[3].descriptor_name | Humans |
| mesh[4].qualifier_ui | Q000235 |
| mesh[4].descriptor_ui | D010957 |
| mesh[4].is_major_topic | False |
| mesh[4].qualifier_name | genetics |
| mesh[4].descriptor_name | Plasmids |
| mesh[5].qualifier_ui | Q000382 |
| mesh[5].descriptor_ui | D002690 |
| mesh[5].is_major_topic | False |
| mesh[5].qualifier_name | microbiology |
| mesh[5].descriptor_name | Chlamydia Infections |
| mesh[6].qualifier_ui | Q000235 |
| mesh[6].descriptor_ui | D002690 |
| mesh[6].is_major_topic | False |
| mesh[6].qualifier_name | genetics |
| mesh[6].descriptor_name | Chlamydia Infections |
| mesh[7].qualifier_ui | Q000235 |
| mesh[7].descriptor_ui | D023502 |
| mesh[7].is_major_topic | False |
| mesh[7].qualifier_name | genetics |
| mesh[7].descriptor_name | Chlamydia muridarum |
| mesh[8].qualifier_ui | |
| mesh[8].descriptor_ui | D017353 |
| mesh[8].is_major_topic | True |
| mesh[8].qualifier_name | |
| mesh[8].descriptor_name | Gene Deletion |
| mesh[9].qualifier_ui | Q000235 |
| mesh[9].descriptor_ui | D002692 |
| mesh[9].is_major_topic | True |
| mesh[9].qualifier_name | genetics |
| mesh[9].descriptor_name | Chlamydia trachomatis |
| mesh[10].qualifier_ui | Q000235 |
| mesh[10].descriptor_ui | D010582 |
| mesh[10].is_major_topic | True |
| mesh[10].qualifier_name | genetics |
| mesh[10].descriptor_name | Bacteriophage lambda |
| mesh[11].qualifier_ui | |
| mesh[11].descriptor_ui | D006801 |
| mesh[11].is_major_topic | False |
| mesh[11].qualifier_name | |
| mesh[11].descriptor_name | Humans |
| mesh[12].qualifier_ui | Q000235 |
| mesh[12].descriptor_ui | D010957 |
| mesh[12].is_major_topic | False |
| mesh[12].qualifier_name | genetics |
| mesh[12].descriptor_name | Plasmids |
| mesh[13].qualifier_ui | Q000382 |
| mesh[13].descriptor_ui | D002690 |
| mesh[13].is_major_topic | False |
| mesh[13].qualifier_name | microbiology |
| mesh[13].descriptor_name | Chlamydia Infections |
| mesh[14].qualifier_ui | Q000235 |
| mesh[14].descriptor_ui | D002690 |
| mesh[14].is_major_topic | False |
| mesh[14].qualifier_name | genetics |
| mesh[14].descriptor_name | Chlamydia Infections |
| mesh[15].qualifier_ui | Q000235 |
| mesh[15].descriptor_ui | D023502 |
| mesh[15].is_major_topic | False |
| mesh[15].qualifier_name | genetics |
| mesh[15].descriptor_name | Chlamydia muridarum |
| mesh[16].qualifier_ui | |
| mesh[16].descriptor_ui | D017353 |
| mesh[16].is_major_topic | True |
| mesh[16].qualifier_name | |
| mesh[16].descriptor_name | Gene Deletion |
| mesh[17].qualifier_ui | Q000235 |
| mesh[17].descriptor_ui | D002692 |
| mesh[17].is_major_topic | True |
| mesh[17].qualifier_name | genetics |
| mesh[17].descriptor_name | Chlamydia trachomatis |
| mesh[18].qualifier_ui | Q000235 |
| mesh[18].descriptor_ui | D010582 |
| mesh[18].is_major_topic | True |
| mesh[18].qualifier_name | genetics |
| mesh[18].descriptor_name | Bacteriophage lambda |
| mesh[19].qualifier_ui | |
| mesh[19].descriptor_ui | D006801 |
| mesh[19].is_major_topic | False |
| mesh[19].qualifier_name | |
| mesh[19].descriptor_name | Humans |
| mesh[20].qualifier_ui | Q000235 |
| mesh[20].descriptor_ui | D010957 |
| mesh[20].is_major_topic | False |
| mesh[20].qualifier_name | genetics |
| mesh[20].descriptor_name | Plasmids |
| mesh[21].qualifier_ui | Q000382 |
| mesh[21].descriptor_ui | D002690 |
| mesh[21].is_major_topic | False |
| mesh[21].qualifier_name | microbiology |
| mesh[21].descriptor_name | Chlamydia Infections |
| mesh[22].qualifier_ui | Q000235 |
| mesh[22].descriptor_ui | D002690 |
| mesh[22].is_major_topic | False |
| mesh[22].qualifier_name | genetics |
| mesh[22].descriptor_name | Chlamydia Infections |
| mesh[23].qualifier_ui | Q000235 |
| mesh[23].descriptor_ui | D023502 |
| mesh[23].is_major_topic | False |
| mesh[23].qualifier_name | genetics |
| mesh[23].descriptor_name | Chlamydia muridarum |
| mesh[24].qualifier_ui | |
| mesh[24].descriptor_ui | D017353 |
| mesh[24].is_major_topic | True |
| mesh[24].qualifier_name | |
| mesh[24].descriptor_name | Gene Deletion |
| mesh[25].qualifier_ui | Q000235 |
| mesh[25].descriptor_ui | D002692 |
| mesh[25].is_major_topic | True |
| mesh[25].qualifier_name | genetics |
| mesh[25].descriptor_name | Chlamydia trachomatis |
| mesh[26].qualifier_ui | Q000235 |
| mesh[26].descriptor_ui | D010582 |
| mesh[26].is_major_topic | True |
| mesh[26].qualifier_name | genetics |
| mesh[26].descriptor_name | Bacteriophage lambda |
| mesh[27].qualifier_ui | |
| mesh[27].descriptor_ui | D006801 |
| mesh[27].is_major_topic | False |
| mesh[27].qualifier_name | |
| mesh[27].descriptor_name | Humans |
| mesh[28].qualifier_ui | Q000235 |
| mesh[28].descriptor_ui | D010957 |
| mesh[28].is_major_topic | False |
| mesh[28].qualifier_name | genetics |
| mesh[28].descriptor_name | Plasmids |
| mesh[29].qualifier_ui | Q000382 |
| mesh[29].descriptor_ui | D002690 |
| mesh[29].is_major_topic | False |
| mesh[29].qualifier_name | microbiology |
| mesh[29].descriptor_name | Chlamydia Infections |
| mesh[30].qualifier_ui | Q000235 |
| mesh[30].descriptor_ui | D002690 |
| mesh[30].is_major_topic | False |
| mesh[30].qualifier_name | genetics |
| mesh[30].descriptor_name | Chlamydia Infections |
| mesh[31].qualifier_ui | Q000235 |
| mesh[31].descriptor_ui | D023502 |
| mesh[31].is_major_topic | False |
| mesh[31].qualifier_name | genetics |
| mesh[31].descriptor_name | Chlamydia muridarum |
| type | article |
| title | Development of a lambda Red based system for gene deletion in Chlamydia |
| awards[0].id | https://openalex.org/G5613624911 |
| awards[0].funder_id | https://openalex.org/F4320338412 |
| awards[0].display_name | |
| awards[0].funder_award_id | R01AI126785 |
| awards[0].funder_display_name | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases |
| biblio.issue | 11 |
| biblio.volume | 19 |
| biblio.last_page | e0311630 |
| biblio.first_page | e0311630 |
| topics[0].id | https://openalex.org/T10428 |
| topics[0].field.id | https://openalex.org/fields/24 |
| topics[0].field.display_name | Immunology and Microbiology |
| topics[0].score | 0.9995999932289124 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/2404 |
| topics[0].subfield.display_name | Microbiology |
| topics[0].display_name | Reproductive tract infections research |
| topics[1].id | https://openalex.org/T12934 |
| topics[1].field.id | https://openalex.org/fields/11 |
| topics[1].field.display_name | Agricultural and Biological Sciences |
| topics[1].score | 0.9872000217437744 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1105 |
| topics[1].subfield.display_name | Ecology, Evolution, Behavior and Systematics |
| topics[1].display_name | Plant and fungal interactions |
| topics[2].id | https://openalex.org/T10066 |
| topics[2].field.id | https://openalex.org/fields/13 |
| topics[2].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[2].score | 0.9470999836921692 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1312 |
| topics[2].subfield.display_name | Molecular Biology |
| topics[2].display_name | Gut microbiota and health |
| funders[0].id | https://openalex.org/F4320338412 |
| funders[0].ror | |
| funders[0].display_name | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases |
| is_xpac | False |
| apc_list.value | 1805 |
| apc_list.currency | USD |
| apc_list.value_usd | 1805 |
| apc_paid.value | 1805 |
| apc_paid.currency | USD |
| apc_paid.value_usd | 1805 |
| concepts[0].id | https://openalex.org/C70239388 |
| concepts[0].level | 4 |
| concepts[0].score | 0.8164531588554382 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q920203 |
| concepts[0].display_name | Recombineering |
| concepts[1].id | https://openalex.org/C86803240 |
| concepts[1].level | 0 |
| concepts[1].score | 0.7723485231399536 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[1].display_name | Biology |
| concepts[2].id | https://openalex.org/C2777391075 |
| concepts[2].level | 2 |
| concepts[2].score | 0.744362473487854 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q131065 |
| concepts[2].display_name | Chlamydia trachomatis |
| concepts[3].id | https://openalex.org/C104317684 |
| concepts[3].level | 2 |
| concepts[3].score | 0.6227394342422485 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[3].display_name | Gene |
| concepts[4].id | https://openalex.org/C141231307 |
| concepts[4].level | 3 |
| concepts[4].score | 0.5327173471450806 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q7020 |
| concepts[4].display_name | Genome |
| concepts[5].id | https://openalex.org/C54355233 |
| concepts[5].level | 1 |
| concepts[5].score | 0.515572726726532 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[5].display_name | Genetics |
| concepts[6].id | https://openalex.org/C22744801 |
| concepts[6].level | 3 |
| concepts[6].score | 0.46272698044776917 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q172778 |
| concepts[6].display_name | Plasmid |
| concepts[7].id | https://openalex.org/C70721500 |
| concepts[7].level | 1 |
| concepts[7].score | 0.33812856674194336 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q177005 |
| concepts[7].display_name | Computational biology |
| concepts[8].id | https://openalex.org/C159047783 |
| concepts[8].level | 1 |
| concepts[8].score | 0.2708479166030884 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q7215 |
| concepts[8].display_name | Virology |
| keywords[0].id | https://openalex.org/keywords/recombineering |
| keywords[0].score | 0.8164531588554382 |
| keywords[0].display_name | Recombineering |
| keywords[1].id | https://openalex.org/keywords/biology |
| keywords[1].score | 0.7723485231399536 |
| keywords[1].display_name | Biology |
| keywords[2].id | https://openalex.org/keywords/chlamydia-trachomatis |
| keywords[2].score | 0.744362473487854 |
| keywords[2].display_name | Chlamydia trachomatis |
| keywords[3].id | https://openalex.org/keywords/gene |
| keywords[3].score | 0.6227394342422485 |
| keywords[3].display_name | Gene |
| keywords[4].id | https://openalex.org/keywords/genome |
| keywords[4].score | 0.5327173471450806 |
| keywords[4].display_name | Genome |
| keywords[5].id | https://openalex.org/keywords/genetics |
| keywords[5].score | 0.515572726726532 |
| keywords[5].display_name | Genetics |
| keywords[6].id | https://openalex.org/keywords/plasmid |
| keywords[6].score | 0.46272698044776917 |
| keywords[6].display_name | Plasmid |
| keywords[7].id | https://openalex.org/keywords/computational-biology |
| keywords[7].score | 0.33812856674194336 |
| keywords[7].display_name | Computational biology |
| keywords[8].id | https://openalex.org/keywords/virology |
| keywords[8].score | 0.2708479166030884 |
| keywords[8].display_name | Virology |
| language | en |
| locations[0].id | doi:10.1371/journal.pone.0311630 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S202381698 |
| locations[0].source.issn | 1932-6203 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 1932-6203 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | PLoS ONE |
| locations[0].source.host_organization | https://openalex.org/P4310315706 |
| locations[0].source.host_organization_name | Public Library of Science |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310315706 |
| locations[0].source.host_organization_lineage_names | Public Library of Science |
| locations[0].license | cc-by |
| locations[0].pdf_url | |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | PLOS ONE |
| locations[0].landing_page_url | https://doi.org/10.1371/journal.pone.0311630 |
| locations[1].id | pmid:39541273 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | PloS one |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/39541273 |
| locations[2].id | pmh:oai:doaj.org/article:3cfbad8539a347429c01a29500bdf45d |
| locations[2].is_oa | False |
| locations[2].source.id | https://openalex.org/S4306401280 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[2].source.host_organization | |
| locations[2].source.host_organization_name | |
| locations[2].license | |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | article |
| locations[2].license_id | |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | PLoS ONE, Vol 19, Iss 11, p e0311630 (2024) |
| locations[2].landing_page_url | https://doaj.org/article/3cfbad8539a347429c01a29500bdf45d |
| locations[3].id | pmh:oai:pubmedcentral.nih.gov:11563418 |
| locations[3].is_oa | True |
| locations[3].source.id | https://openalex.org/S2764455111 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | PubMed Central |
| locations[3].source.host_organization | https://openalex.org/I1299303238 |
| locations[3].source.host_organization_name | National Institutes of Health |
| locations[3].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[3].license | other-oa |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | Text |
| locations[3].license_id | https://openalex.org/licenses/other-oa |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | PLoS One |
| locations[3].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/11563418 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5100664212 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-0012-4455 |
| authorships[0].author.display_name | Yibing Wang |
| authorships[0].countries | US |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I201448701 |
| authorships[0].affiliations[0].raw_affiliation_string | Department of Medicine, University of Washington, Seattle, Washington, United States of America |
| authorships[0].institutions[0].id | https://openalex.org/I201448701 |
| authorships[0].institutions[0].ror | https://ror.org/00cvxb145 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I201448701 |
| authorships[0].institutions[0].country_code | US |
| authorships[0].institutions[0].display_name | University of Washington |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Yibing Wang |
| authorships[0].is_corresponding | True |
| authorships[0].raw_affiliation_strings | Department of Medicine, University of Washington, Seattle, Washington, United States of America |
| authorships[1].author.id | https://openalex.org/A5056061919 |
| authorships[1].author.orcid | |
| authorships[1].author.display_name | Robert J. Suchland |
| authorships[1].countries | US |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I201448701 |
| authorships[1].affiliations[0].raw_affiliation_string | Department of Medicine, University of Washington, Seattle, Washington, United States of America |
| authorships[1].institutions[0].id | https://openalex.org/I201448701 |
| authorships[1].institutions[0].ror | https://ror.org/00cvxb145 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I201448701 |
| authorships[1].institutions[0].country_code | US |
| authorships[1].institutions[0].display_name | University of Washington |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Robert Suchland |
| authorships[1].is_corresponding | True |
| authorships[1].raw_affiliation_strings | Department of Medicine, University of Washington, Seattle, Washington, United States of America |
| authorships[2].author.id | https://openalex.org/A5061755400 |
| authorships[2].author.orcid | https://orcid.org/0009-0007-8093-7125 |
| authorships[2].author.display_name | Amy Hua |
| authorships[2].countries | US |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I131249849 |
| authorships[2].affiliations[0].raw_affiliation_string | Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, United States of America |
| authorships[2].institutions[0].id | https://openalex.org/I131249849 |
| authorships[2].institutions[0].ror | https://ror.org/00ysfqy60 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I131249849 |
| authorships[2].institutions[0].country_code | US |
| authorships[2].institutions[0].display_name | Oregon State University |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Amy Hua |
| authorships[2].is_corresponding | True |
| authorships[2].raw_affiliation_strings | Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, United States of America |
| authorships[3].author.id | https://openalex.org/A5006546528 |
| authorships[3].author.orcid | |
| authorships[3].author.display_name | Steven Carrell |
| authorships[3].countries | US |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I131249849 |
| authorships[3].affiliations[0].raw_affiliation_string | Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, United States of America |
| authorships[3].institutions[0].id | https://openalex.org/I131249849 |
| authorships[3].institutions[0].ror | https://ror.org/00ysfqy60 |
| authorships[3].institutions[0].type | education |
| authorships[3].institutions[0].lineage | https://openalex.org/I131249849 |
| authorships[3].institutions[0].country_code | US |
| authorships[3].institutions[0].display_name | Oregon State University |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Steven Carrell |
| authorships[3].is_corresponding | True |
| authorships[3].raw_affiliation_strings | Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, United States of America |
| authorships[4].author.id | https://openalex.org/A5081751244 |
| authorships[4].author.orcid | https://orcid.org/0000-0003-0663-9916 |
| authorships[4].author.display_name | Daniel D. Rockey |
| authorships[4].countries | US |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I131249849 |
| authorships[4].affiliations[0].raw_affiliation_string | Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, United States of America |
| authorships[4].institutions[0].id | https://openalex.org/I131249849 |
| authorships[4].institutions[0].ror | https://ror.org/00ysfqy60 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I131249849 |
| authorships[4].institutions[0].country_code | US |
| authorships[4].institutions[0].display_name | Oregon State University |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Daniel Rockey |
| authorships[4].is_corresponding | True |
| authorships[4].raw_affiliation_strings | Department of Biomedical Sciences, Oregon State University, Corvallis, Oregon, United States of America |
| authorships[5].author.id | https://openalex.org/A5041716321 |
| authorships[5].author.orcid | https://orcid.org/0000-0002-2967-3079 |
| authorships[5].author.display_name | Kevin Hybiske |
| authorships[5].countries | US |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I201448701 |
| authorships[5].affiliations[0].raw_affiliation_string | Department of Medicine, University of Washington, Seattle, Washington, United States of America |
| authorships[5].institutions[0].id | https://openalex.org/I201448701 |
| authorships[5].institutions[0].ror | https://ror.org/00cvxb145 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I201448701 |
| authorships[5].institutions[0].country_code | US |
| authorships[5].institutions[0].display_name | University of Washington |
| authorships[5].author_position | last |
| authorships[5].raw_author_name | Kevin Hybiske |
| authorships[5].is_corresponding | True |
| authorships[5].raw_affiliation_strings | Department of Medicine, University of Washington, Seattle, Washington, United States of America |
| has_content.pdf | False |
| has_content.grobid_xml | False |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://doi.org/10.1371/journal.pone.0311630 |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Development of a lambda Red based system for gene deletion in Chlamydia |
| has_fulltext | False |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10428 |
| primary_topic.field.id | https://openalex.org/fields/24 |
| primary_topic.field.display_name | Immunology and Microbiology |
| primary_topic.score | 0.9995999932289124 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/2404 |
| primary_topic.subfield.display_name | Microbiology |
| primary_topic.display_name | Reproductive tract infections research |
| related_works | https://openalex.org/W3049761894, https://openalex.org/W1995164683, https://openalex.org/W3217106447, https://openalex.org/W1537398925, https://openalex.org/W2396093027, https://openalex.org/W4223470765, https://openalex.org/W3036564801, https://openalex.org/W143773786, https://openalex.org/W4234324951, https://openalex.org/W4214898270 |
| cited_by_count | 3 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 3 |
| locations_count | 4 |
| best_oa_location.id | doi:10.1371/journal.pone.0311630 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S202381698 |
| best_oa_location.source.issn | 1932-6203 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 1932-6203 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | PLoS ONE |
| best_oa_location.source.host_organization | https://openalex.org/P4310315706 |
| best_oa_location.source.host_organization_name | Public Library of Science |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310315706 |
| best_oa_location.source.host_organization_lineage_names | Public Library of Science |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | PLOS ONE |
| best_oa_location.landing_page_url | https://doi.org/10.1371/journal.pone.0311630 |
| primary_location.id | doi:10.1371/journal.pone.0311630 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S202381698 |
| primary_location.source.issn | 1932-6203 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 1932-6203 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | PLoS ONE |
| primary_location.source.host_organization | https://openalex.org/P4310315706 |
| primary_location.source.host_organization_name | Public Library of Science |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310315706 |
| primary_location.source.host_organization_lineage_names | Public Library of Science |
| primary_location.license | cc-by |
| primary_location.pdf_url | |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | PLOS ONE |
| primary_location.landing_page_url | https://doi.org/10.1371/journal.pone.0311630 |
| publication_date | 2024-11-14 |
| publication_year | 2024 |
| referenced_works | https://openalex.org/W2523562739, https://openalex.org/W3125062323, https://openalex.org/W1968572317, https://openalex.org/W2116059401, https://openalex.org/W2247315273, https://openalex.org/W2332232022, https://openalex.org/W2077269211, https://openalex.org/W2156718046, https://openalex.org/W2143608477, https://openalex.org/W2094119638, https://openalex.org/W2259151740, https://openalex.org/W2966309307, https://openalex.org/W2973063509, https://openalex.org/W3154164226, https://openalex.org/W2973043909, https://openalex.org/W2034054997, https://openalex.org/W4303986639, https://openalex.org/W1884874540, https://openalex.org/W2136670217, https://openalex.org/W2116137883, https://openalex.org/W2071096810, https://openalex.org/W1662450356, https://openalex.org/W1598802930, https://openalex.org/W2001958313, https://openalex.org/W4285150126, https://openalex.org/W2111967267, https://openalex.org/W2316846577, https://openalex.org/W2897257008, https://openalex.org/W2951776284, https://openalex.org/W2146888113, https://openalex.org/W2120879140, https://openalex.org/W2602967040, https://openalex.org/W2230574554, https://openalex.org/W2343929623, https://openalex.org/W2119766894, https://openalex.org/W2163327255, https://openalex.org/W2114593800, https://openalex.org/W4200015580, https://openalex.org/W4387950425, https://openalex.org/W2891727496 |
| referenced_works_count | 40 |
| abstract_inverted_index.) | 125 |
| abstract_inverted_index., | 191 |
| abstract_inverted_index.. | 44, 72, 77, 79, 93, 185, 189, 219 |
| abstract_inverted_index.2 | 112 |
| abstract_inverted_index.3 | 107 |
| abstract_inverted_index.5 | 178 |
| abstract_inverted_index.C | 71, 76, 92, 184, 188 |
| abstract_inverted_index.a | 14, 37, 46, 100, 206 |
| abstract_inverted_index.To | 169 |
| abstract_inverted_index.We | 199 |
| abstract_inverted_index.be | 25 |
| abstract_inverted_index.by | 128, 146 |
| abstract_inverted_index.in | 6, 17, 66, 116, 183, 217 |
| abstract_inverted_index.of | 36, 62, 83, 91, 109, 114, 137, 153, 160, 173, 194 |
| abstract_inverted_index.or | 111 |
| abstract_inverted_index.to | 2, 24, 204 |
| abstract_inverted_index.we | 30, 57, 86, 176 |
| abstract_inverted_index.For | 80 |
| abstract_inverted_index.Red | 52, 202 |
| abstract_inverted_index.The | 0 |
| abstract_inverted_index.and | 20, 27, 34, 54, 75, 119, 131, 149, 156, 187, 196 |
| abstract_inverted_index.for | 11, 42, 95, 210 |
| abstract_inverted_index.key | 50 |
| abstract_inverted_index.new | 21, 208 |
| abstract_inverted_index.red | 39 |
| abstract_inverted_index.the | 7, 18, 32, 59, 67, 84, 88, 120, 151, 157, 171 |
| abstract_inverted_index.was | 144 |
| abstract_inverted_index.CTΔ | 142, 165 |
| abstract_inverted_index.Here | 29 |
| abstract_inverted_index.IncA | 154 |
| abstract_inverted_index.gene | 64, 90, 212 |
| abstract_inverted_index.goal | 16 |
| abstract_inverted_index.host | 138 |
| abstract_inverted_index.incA | 89, 124, 143, 166 |
| abstract_inverted_index.lack | 152 |
| abstract_inverted_index.loci | 5 |
| abstract_inverted_index.need | 23 |
| abstract_inverted_index.this | 174 |
| abstract_inverted_index.were | 104, 126 |
| abstract_inverted_index.with | 141 |
| abstract_inverted_index.(CTΔ | 123 |
| abstract_inverted_index.McCoy | 117 |
| abstract_inverted_index.Using | 45 |
| abstract_inverted_index.cells | 118, 139 |
| abstract_inverted_index.field | 19 |
| abstract_inverted_index.model | 68 |
| abstract_inverted_index.offer | 205 |
| abstract_inverted_index.whole | 132 |
| abstract_inverted_index.L2/434 | 74 |
| abstract_inverted_index.across | 164 |
| abstract_inverted_index.and/or | 214 |
| abstract_inverted_index.clones | 122 |
| abstract_inverted_index.expect | 200 |
| abstract_inverted_index.genes. | 198 |
| abstract_inverted_index.genome | 10, 133 |
| abstract_inverted_index.lambda | 38, 51, 201 |
| abstract_inverted_index.making | 211 |
| abstract_inverted_index.marker | 103 |
| abstract_inverted_index.rounds | 108, 113 |
| abstract_inverted_index.single | 195 |
| abstract_inverted_index.system | 41 |
| abstract_inverted_index.target | 4 |
| abstract_inverted_index.within | 106 |
| abstract_inverted_index.ability | 1 |
| abstract_inverted_index.deleted | 177 |
| abstract_inverted_index.explore | 170 |
| abstract_inverted_index.factors | 182 |
| abstract_inverted_index.initial | 81 |
| abstract_inverted_index.mutants | 98, 216 |
| abstract_inverted_index.plasmid | 48 |
| abstract_inverted_index.remains | 13 |
| abstract_inverted_index.strains | 70 |
| abstract_inverted_index.system, | 85, 175 |
| abstract_inverted_index.targets | 65 |
| abstract_inverted_index.uniform | 158 |
| abstract_inverted_index.utility | 172 |
| abstract_inverted_index.Deletion | 97 |
| abstract_inverted_index.deletion | 12, 61, 213 |
| abstract_inverted_index.describe | 31 |
| abstract_inverted_index.encoding | 49 |
| abstract_inverted_index.infected | 140 |
| abstract_inverted_index.multiple | 197 |
| abstract_inverted_index.numerous | 63 |
| abstract_inverted_index.powerful | 207 |
| abstract_inverted_index.presence | 159 |
| abstract_inverted_index.refined. | 28 |
| abstract_inverted_index.strategy | 209 |
| abstract_inverted_index.targeted | 87 |
| abstract_inverted_index.vacuoles | 162 |
| abstract_inverted_index.verified | 127 |
| abstract_inverted_index.-infected | 167 |
| abstract_inverted_index.Chlamydia | 8, 43, 218 |
| abstract_inverted_index.PCR-based | 129 |
| abstract_inverted_index.candidate | 180 |
| abstract_inverted_index.confirmed | 150 |
| abstract_inverted_index.deletion. | 96 |
| abstract_inverted_index.deletions | 193 |
| abstract_inverted_index.desirable | 15 |
| abstract_inverted_index.developed | 26 |
| abstract_inverted_index.efficient | 60 |
| abstract_inverted_index.including | 192 |
| abstract_inverted_index.muridarum | 78, 190 |
| abstract_inverted_index.performed | 145 |
| abstract_inverted_index.recovered | 105 |
| abstract_inverted_index.resulting | 121 |
| abstract_inverted_index.targeting | 55 |
| abstract_inverted_index.virulence | 181 |
| abstract_inverted_index.Phenotypic | 135 |
| abstract_inverted_index.additional | 179 |
| abstract_inverted_index.assessment | 136 |
| abstract_inverted_index.chlamydial | 69 |
| abstract_inverted_index.components | 53 |
| abstract_inverted_index.containing | 99 |
| abstract_inverted_index.expression | 155 |
| abstract_inverted_index.genotyping | 130 |
| abstract_inverted_index.microscopy | 148 |
| abstract_inverted_index.passaging, | 115 |
| abstract_inverted_index.resistance | 102 |
| abstract_inverted_index.selection, | 110 |
| abstract_inverted_index.sequences, | 56 |
| abstract_inverted_index.strategies | 22 |
| abstract_inverted_index.application | 35 |
| abstract_inverted_index.demonstrate | 58 |
| abstract_inverted_index.development | 33, 82 |
| abstract_inverted_index.efficiently | 3 |
| abstract_inverted_index.monolayers. | 168 |
| abstract_inverted_index.replacement | 215 |
| abstract_inverted_index.sequencing. | 134 |
| abstract_inverted_index.trachomatis | 9, 73, 94, 186 |
| abstract_inverted_index.(inclusions) | 163 |
| abstract_inverted_index.nonfusogenic | 161 |
| abstract_inverted_index.recombineering | 40, 203 |
| abstract_inverted_index.chloramphenicol | 101 |
| abstract_inverted_index.non-replicative | 47 |
| abstract_inverted_index.immunofluorescence | 147 |
| cited_by_percentile_year.max | 97 |
| cited_by_percentile_year.min | 96 |
| corresponding_author_ids | https://openalex.org/A5081751244, https://openalex.org/A5100664212, https://openalex.org/A5056061919, https://openalex.org/A5061755400, https://openalex.org/A5006546528, https://openalex.org/A5041716321 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 6 |
| corresponding_institution_ids | https://openalex.org/I131249849, https://openalex.org/I201448701 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/15 |
| sustainable_development_goals[0].score | 0.5299999713897705 |
| sustainable_development_goals[0].display_name | Life in Land |
| citation_normalized_percentile.value | 0.81764142 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |