Development of a Simple, Low-Cost, Highly Specific Colorimetry-Based Whole-Cell Bacterial Biosensor for Cadmium Detection in the Environment Article Swipe

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Rajkumar Prabhakaran , Tharmarajan Ramprasath , Prakash Shyam Karuppiah , Govindan Sadasivam Selvam ·

YOU? · · 2024 · Open Access · · DOI: https://doi.org/10.21203/rs.3.rs-4222259/v1

The urgent requirement for cost-effective technology to detect heavy metals in the environment is undeniable. This study introduces a novel approach: a low-cost colorimetry-based optical whole-cell bacterial biosensor utilizing a newly identified reporter gene, agarase (dagA), for the specific detection of cadmium (Cd). Through the incorporation of a Cd-sensing promoter (P_cadR) upstream of agarase in Pseudomonas aeruginosa, a highly specific Cd biosensor was engineered. Initially developed in E. coli and subsequently in P. aeruginosa, these agarase (dagA) based optical whole-cell bacterial biosensors exhibited promising capabilities. Specifically, P. aeruginosa carrying the vector P_cadR-dagA demonstrated a strong linear range detection for cadmium chloride concentrations ranging from 10 ppb to 500 ppm (R² = 0.9851), with a correlation of 0.96 (Bland-Altman) to the standard inductively coupled plasma mass spectroscopy method. Furthermore, morphological alterations were observed through scanning electron microscopy. Notably, this colorimetry-based optical whole-cell bacterial biosensor presents itself as a straightforward, resilient, portable, and cost-effective solution, eliminating the need for complex sample preparation. Consequently, the biosensor platform proposed in this study stands as a viable, low-cost alternative for monitoring low levels of Cd in the environment.

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Biosensor Colorimetry Chemistry Nanotechnology Chromatography Materials science Biochemistry

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Type: preprint
Language: en
Landing Page: https://doi.org/10.21203/rs.3.rs-4222259/v1
PDF: https://www.researchsquare.com/article/rs-4222259/latest.pdf
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References: 23
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DOI: https://doi.org/10.21203/rs.3.rs-4222259/v1

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Title: Development of a Simple, Low-Cost, Highly Specific Colorimetry-Based Whole-Cell Bacterial Biosensor for Cadmium Detection in the Environment

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Type: preprint

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Language: en

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Publication year: 2024

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Publication date: 2024-05-30

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Authors: Rajkumar Prabhakaran, Tharmarajan Ramprasath, Prakash Shyam Karuppiah, Govindan Sadasivam Selvam

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Landing page: https://doi.org/10.21203/rs.3.rs-4222259/v1

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Concepts: Biosensor, Colorimetry, Chemistry, Nanotechnology, Chromatography, Materials science, Biochemistry

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