Development of an Automated, Ultra-Rapid Bottom-Up Proteomics Workflow Utilizing Alginate-Based Hydrogels Article Swipe
Maor Arad
,
Connor Frey
,
Ronald Balagtas
,
Rhien Hare
,
Kenneth Ku
,
Dario Jereb
,
Zach Nestman
,
Arshdeep Sidhu
,
Yuming Shi
,
Osei Boakye Fordwour
,
Kyung‐Mee Moon
,
Leonard J. Foster
,
Golfam Ghafourifar
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1021/acs.analchem.4c04846
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1021/acs.analchem.4c04846
A new approach to sample preparation and enzymatic digestion in bottom-up proteomics has been developed using alginate-based hydrogel entrapment of enzymes. This hydrogel facilitates rapid and room-temperature digestions with multienzyme capabilities. Three methodologies were tested: within microcentrifuge tubes, in situ pipette tips, and automated robotic liquid handling. Factorial experimental design identified a 1 h, room temperature, pepsin-trypsin dual-enzyme digestion as optimal for sequence coverage and protein group identification, comparable to a gold-standard overnight proteomic protocol. This method promises significant advancements in proteomic analysis by enhancing reusability, speed, throughput, convenience, and cost-effectiveness, without hindering digestion efficiency.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1021/acs.analchem.4c04846
- https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.4c04846?ref=article_openPDF
- OA Status
- hybrid
- Cited By
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- References
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- Related Works
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- OpenAlex ID
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All OpenAlex metadata
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https://openalex.org/W4404235245Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1021/acs.analchem.4c04846Digital Object Identifier
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Development of an Automated, Ultra-Rapid Bottom-Up Proteomics Workflow Utilizing Alginate-Based HydrogelsWork title
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articleOpenAlex work type
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-11-11Full publication date if available
- Authors
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Maor Arad, Connor Frey, Ronald Balagtas, Rhien Hare, Kenneth Ku, Dario Jereb, Zach Nestman, Arshdeep Sidhu, Yuming Shi, Osei Boakye Fordwour, Kyung‐Mee Moon, Leonard J. Foster, Golfam GhafourifarList of authors in order
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https://doi.org/10.1021/acs.analchem.4c04846Publisher landing page
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https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.4c04846?ref=article_openPDFDirect link to full text PDF
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.4c04846?ref=article_openPDFDirect OA link when available
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Chemistry, Self-healing hydrogels, Workflow, Nanotechnology, Polymer chemistry, Computer science, Database, Materials scienceTop concepts (fields/topics) attached by OpenAlex
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4Total citation count in OpenAlex
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2025: 4Per-year citation counts (last 5 years)
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42Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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| referenced_works | https://openalex.org/W4403381371, https://openalex.org/W4366442044, https://openalex.org/W3115609147, https://openalex.org/W4378575828, https://openalex.org/W3106602244, https://openalex.org/W4309164523, https://openalex.org/W2954182729, https://openalex.org/W3155516421, https://openalex.org/W1561283795, https://openalex.org/W2039227708, https://openalex.org/W4399361084, https://openalex.org/W4225259143, https://openalex.org/W4324061310, https://openalex.org/W2279921036, https://openalex.org/W1552928638, https://openalex.org/W2893688084, https://openalex.org/W2550622545, https://openalex.org/W1991366673, https://openalex.org/W1986612337, https://openalex.org/W4297124146, https://openalex.org/W2914535622, https://openalex.org/W4393204883, https://openalex.org/W4387252265, https://openalex.org/W3139290549, https://openalex.org/W3084724826, https://openalex.org/W3214632622, https://openalex.org/W4384037845, https://openalex.org/W1971500643, https://openalex.org/W2790709038, https://openalex.org/W2128635872, https://openalex.org/W2078594804, https://openalex.org/W2076358214, https://openalex.org/W2766946295, https://openalex.org/W4220831425, https://openalex.org/W2143079975, https://openalex.org/W2050345155, https://openalex.org/W2952615074, https://openalex.org/W3208691473, https://openalex.org/W2069201501, https://openalex.org/W2084479022, https://openalex.org/W2022177330, https://openalex.org/W2042095100 |
| referenced_works_count | 42 |
| abstract_inverted_index.1 | 52 |
| abstract_inverted_index.A | 0 |
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| corresponding_author_ids | https://openalex.org/A5058742097, https://openalex.org/A5044695259 |
| countries_distinct_count | 2 |
| institutions_distinct_count | 13 |
| corresponding_institution_ids | https://openalex.org/I141945490, https://openalex.org/I170606269, https://openalex.org/I4210133112 |
| citation_normalized_percentile.value | 0.79686991 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |