Development of RP HPLC‐PDA method for simultaneous quantitative analysis of Inoscavin A and Meshimakobnol A and application on some Phellinus mushroom species
Article Swipe
Ngoc-Anh Le
,
Nguyễn Ngọc Tuân
,
Dong Thi Anh Dao
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1002/fsn3.4031
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.1002/fsn3.4031
Phellinus igniarius , a medicinal mushroom containing many active ingredients with health benefits, can be applied in functional food. At present, the quantification of the main active ingredients from higher fungi ( Ganoderma , Phellinus… ) materials from different growing sources is a mandatory requirement to standardize the input resources of pharmaceutical and food production. Our study's aims are to perfect the RP HPLC‐PDA method for quantitative analysis of Inoscavin A and Meshimakobnol A which are two main active ingredients present in Phellinus mushroom. In this analytical method, a C18‐HPLC column and the mixture of methanol and formic acid solutions (pH = 2.2) are used to analyze and elute the active substances with the column activity parameters being the concentration gradient. This perfect method was tested for system suitability, repeatability, intermediate precision, recovery, and linear curve calibration to validate the method. After validation, the perfected RP HPLC‐PDA method was applied to analyze eight samples of Phellinus and three samples of Ganoderma mushroom category . This method can be the basis for classifying between Phellinus and some other medicinal mushrooms.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1002/fsn3.4031
- OA Status
- hybrid
- Cited By
- 1
- References
- 31
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4392039037
All OpenAlex metadata
Raw OpenAlex JSON
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https://openalex.org/W4392039037Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.1002/fsn3.4031Digital Object Identifier
- Title
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Development of
RP HPLC‐PDA method for simultaneous quantitative analysis of Inoscavin A and Meshimakobnol A and application on some Phellinus mushroom speciesWork title - Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-02-22Full publication date if available
- Authors
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Ngoc-Anh Le, Nguyễn Ngọc Tuân, Dong Thi Anh DaoList of authors in order
- Landing page
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https://doi.org/10.1002/fsn3.4031Publisher landing page
- Open access
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YesWhether a free full text is available
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hybridOpen access status per OpenAlex
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https://doi.org/10.1002/fsn3.4031Direct OA link when available
- Concepts
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Ganoderma, Mushroom, High-performance liquid chromatography, Repeatability, Chromatography, Formic acid, Gradient elution, Chemistry, Ganoderma lucidum, Active ingredient, Calibration curve, Food science, Biology, Detection limit, BioinformaticsTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
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