Discovery of a New Natural Product and a Deactivation of a Quorum Sensing System by Culturing a “Producer” Bacterium With a Heat-Killed “Inducer” Culture Article Swipe
Libang Liang
,
Amanda Sproule
,
Bradley Haltli
,
Douglas H. Marchbank
,
Fabrice Berrué
,
David P. Overy
,
Kate McQuillan
,
Martin Lanteigne
,
Noelle J. Duncan
,
Hebelin Correa
,
Russell G. Kerr
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2018.03351
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2018.03351
Herein we describe a modified bacterial culture methodology as a tool to discover new natural products via supplementing actinomycete fermentation media with autoclaved cultures of "inducer" microbes. Using seven actinomycetes and four inducer microbes, we detected 28 metabolites that were induced in UHPLC-HRESIMS-based analysis of bacterial fermentations. Metabolomic analysis indicated that each inducer elicited a unique response from the actinomycetes and that some chemical responses were specific to each inducer-producer combination. Among these 28 metabolites, hydrazidomycin D, a new hydrazide-containing natural product was isolated from the pair Streptomyces sp. RKBH-B178 and Mycobacterium smegmatis. This result validated the effectiveness of the strategy in discovering new natural products. From the same set of induced metabolites, an in-depth investigation of a fermentation of Streptomyces sp. RKBH-B178 and autoclaved Pseudomonas aeruginosa led to the discovery of a glucuronidated analog of the pseudomonas quinolone signal (PQS). We demonstrated that RKBH-B178 is able to biotransform the P. aeruginosa quorum sensing molecules, 2-heptyl-4-quinolone (HHQ), and PQS to form PQS-GlcA. Further, PQS-GlcA was shown to have poor binding affinity to PqsR, the innate receptor of HHQ and PQS.
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fmicb.2018.03351
- OA Status
- gold
- Cited By
- 18
- References
- 73
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2908576524
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W2908576524Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3389/fmicb.2018.03351Digital Object Identifier
- Title
-
Discovery of a New Natural Product and a Deactivation of a Quorum Sensing System by Culturing a “Producer” Bacterium With a Heat-Killed “Inducer” CultureWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2019Year of publication
- Publication date
-
2019-01-17Full publication date if available
- Authors
-
Libang Liang, Amanda Sproule, Bradley Haltli, Douglas H. Marchbank, Fabrice Berrué, David P. Overy, Kate McQuillan, Martin Lanteigne, Noelle J. Duncan, Hebelin Correa, Russell G. KerrList of authors in order
- Landing page
-
https://doi.org/10.3389/fmicb.2018.03351Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.3389/fmicb.2018.03351Direct OA link when available
- Concepts
-
Inducer, Quorum sensing, Natural product, Pseudomonas aeruginosa, Streptomyces, Bacteria, Fermentation, Microbiology, Biochemistry, Biology, Chemistry, Gene, Genetics, BiofilmTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
18Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 2, 2024: 3, 2023: 4, 2022: 2, 2021: 2Per-year citation counts (last 5 years)
- References (count)
-
73Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W2908576524 |
|---|---|
| doi | https://doi.org/10.3389/fmicb.2018.03351 |
| ids.doi | https://doi.org/10.3389/fmicb.2018.03351 |
| ids.mag | 2908576524 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/30705672 |
| ids.openalex | https://openalex.org/W2908576524 |
| fwci | 0.92745907 |
| type | article |
| title | Discovery of a New Natural Product and a Deactivation of a Quorum Sensing System by Culturing a “Producer” Bacterium With a Heat-Killed “Inducer” Culture |
| biblio.issue | |
| biblio.volume | 9 |
| biblio.last_page | 3351 |
| biblio.first_page | 3351 |
| topics[0].id | https://openalex.org/T10593 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.9995999932289124 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1312 |
| topics[0].subfield.display_name | Molecular Biology |
| topics[0].display_name | Bacterial biofilms and quorum sensing |
| topics[1].id | https://openalex.org/T10252 |
| topics[1].field.id | https://openalex.org/fields/27 |
| topics[1].field.display_name | Medicine |
| topics[1].score | 0.9839000105857849 |
| topics[1].domain.id | https://openalex.org/domains/4 |
| topics[1].domain.display_name | Health Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2736 |
| topics[1].subfield.display_name | Pharmacology |
| topics[1].display_name | Microbial Natural Products and Biosynthesis |
| topics[2].id | https://openalex.org/T12561 |
| topics[2].field.id | https://openalex.org/fields/13 |
| topics[2].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[2].score | 0.9807999730110168 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1312 |
| topics[2].subfield.display_name | Molecular Biology |
| topics[2].display_name | bioluminescence and chemiluminescence research |
| is_xpac | False |
| apc_list.value | 2950 |
| apc_list.currency | USD |
| apc_list.value_usd | 2950 |
| apc_paid.value | 2950 |
| apc_paid.currency | USD |
| apc_paid.value_usd | 2950 |
| concepts[0].id | https://openalex.org/C2225953 |
| concepts[0].level | 3 |
| concepts[0].score | 0.8922184705734253 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q1661899 |
| concepts[0].display_name | Inducer |
| concepts[1].id | https://openalex.org/C118687296 |
| concepts[1].level | 4 |
| concepts[1].score | 0.7972562313079834 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q905754 |
| concepts[1].display_name | Quorum sensing |
| concepts[2].id | https://openalex.org/C2779396153 |
| concepts[2].level | 2 |
| concepts[2].score | 0.7786095142364502 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q901227 |
| concepts[2].display_name | Natural product |
| concepts[3].id | https://openalex.org/C2777637488 |
| concepts[3].level | 3 |
| concepts[3].score | 0.6822349429130554 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q31856 |
| concepts[3].display_name | Pseudomonas aeruginosa |
| concepts[4].id | https://openalex.org/C2779920670 |
| concepts[4].level | 3 |
| concepts[4].score | 0.618122398853302 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q1144013 |
| concepts[4].display_name | Streptomyces |
| concepts[5].id | https://openalex.org/C523546767 |
| concepts[5].level | 2 |
| concepts[5].score | 0.5623220205307007 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q10876 |
| concepts[5].display_name | Bacteria |
| concepts[6].id | https://openalex.org/C100544194 |
| concepts[6].level | 2 |
| concepts[6].score | 0.5118626952171326 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q41760 |
| concepts[6].display_name | Fermentation |
| concepts[7].id | https://openalex.org/C89423630 |
| concepts[7].level | 1 |
| concepts[7].score | 0.4532252252101898 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q7193 |
| concepts[7].display_name | Microbiology |
| concepts[8].id | https://openalex.org/C55493867 |
| concepts[8].level | 1 |
| concepts[8].score | 0.4516734480857849 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[8].display_name | Biochemistry |
| concepts[9].id | https://openalex.org/C86803240 |
| concepts[9].level | 0 |
| concepts[9].score | 0.4449818730354309 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[9].display_name | Biology |
| concepts[10].id | https://openalex.org/C185592680 |
| concepts[10].level | 0 |
| concepts[10].score | 0.3861619532108307 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[10].display_name | Chemistry |
| concepts[11].id | https://openalex.org/C104317684 |
| concepts[11].level | 2 |
| concepts[11].score | 0.06981980800628662 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[11].display_name | Gene |
| concepts[12].id | https://openalex.org/C54355233 |
| concepts[12].level | 1 |
| concepts[12].score | 0.0676071047782898 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[12].display_name | Genetics |
| concepts[13].id | https://openalex.org/C58123911 |
| concepts[13].level | 3 |
| concepts[13].score | 0.0 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q467410 |
| concepts[13].display_name | Biofilm |
| keywords[0].id | https://openalex.org/keywords/inducer |
| keywords[0].score | 0.8922184705734253 |
| keywords[0].display_name | Inducer |
| keywords[1].id | https://openalex.org/keywords/quorum-sensing |
| keywords[1].score | 0.7972562313079834 |
| keywords[1].display_name | Quorum sensing |
| keywords[2].id | https://openalex.org/keywords/natural-product |
| keywords[2].score | 0.7786095142364502 |
| keywords[2].display_name | Natural product |
| keywords[3].id | https://openalex.org/keywords/pseudomonas-aeruginosa |
| keywords[3].score | 0.6822349429130554 |
| keywords[3].display_name | Pseudomonas aeruginosa |
| keywords[4].id | https://openalex.org/keywords/streptomyces |
| keywords[4].score | 0.618122398853302 |
| keywords[4].display_name | Streptomyces |
| keywords[5].id | https://openalex.org/keywords/bacteria |
| keywords[5].score | 0.5623220205307007 |
| keywords[5].display_name | Bacteria |
| keywords[6].id | https://openalex.org/keywords/fermentation |
| keywords[6].score | 0.5118626952171326 |
| keywords[6].display_name | Fermentation |
| keywords[7].id | https://openalex.org/keywords/microbiology |
| keywords[7].score | 0.4532252252101898 |
| keywords[7].display_name | Microbiology |
| keywords[8].id | https://openalex.org/keywords/biochemistry |
| keywords[8].score | 0.4516734480857849 |
| keywords[8].display_name | Biochemistry |
| keywords[9].id | https://openalex.org/keywords/biology |
| keywords[9].score | 0.4449818730354309 |
| keywords[9].display_name | Biology |
| keywords[10].id | https://openalex.org/keywords/chemistry |
| keywords[10].score | 0.3861619532108307 |
| keywords[10].display_name | Chemistry |
| keywords[11].id | https://openalex.org/keywords/gene |
| keywords[11].score | 0.06981980800628662 |
| keywords[11].display_name | Gene |
| keywords[12].id | https://openalex.org/keywords/genetics |
| keywords[12].score | 0.0676071047782898 |
| keywords[12].display_name | Genetics |
| language | en |
| locations[0].id | doi:10.3389/fmicb.2018.03351 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S2484704948 |
| locations[0].source.issn | 1664-302X |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 1664-302X |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | Frontiers in Microbiology |
| locations[0].source.host_organization | https://openalex.org/P4310320527 |
| locations[0].source.host_organization_name | Frontiers Media |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310320527 |
| locations[0].source.host_organization_lineage_names | Frontiers Media |
| locations[0].license | cc-by |
| locations[0].pdf_url | |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Frontiers in Microbiology |
| locations[0].landing_page_url | https://doi.org/10.3389/fmicb.2018.03351 |
| locations[1].id | pmid:30705672 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Frontiers in microbiology |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/30705672 |
| locations[2].id | pmh:oai:doaj.org/article:bdb697a2d9de4931a90f644eb0e054cd |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S4306401280 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[2].source.host_organization | |
| locations[2].source.host_organization_name | |
| locations[2].license | cc-by-sa |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | article |
| locations[2].license_id | https://openalex.org/licenses/cc-by-sa |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | Frontiers in Microbiology, Vol 9 (2019) |
| locations[2].landing_page_url | https://doaj.org/article/bdb697a2d9de4931a90f644eb0e054cd |
| locations[3].id | pmh:oai:europepmc.org:5339102 |
| locations[3].is_oa | True |
| locations[3].source.id | https://openalex.org/S4306400806 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | Europe PMC (PubMed Central) |
| locations[3].source.host_organization | https://openalex.org/I1303153112 |
| locations[3].source.host_organization_name | European Bioinformatics Institute |
| locations[3].source.host_organization_lineage | https://openalex.org/I1303153112 |
| locations[3].license | cc-by |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | Text |
| locations[3].license_id | https://openalex.org/licenses/cc-by |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | |
| locations[3].landing_page_url | http://europepmc.org/pmc/articles/PMC6344404 |
| locations[4].id | pmh:oai:islandscholar.ca:ir_22453 |
| locations[4].is_oa | True |
| locations[4].source.id | https://openalex.org/S4306401181 |
| locations[4].source.issn | |
| locations[4].source.type | repository |
| locations[4].source.is_oa | False |
| locations[4].source.issn_l | |
| locations[4].source.is_core | False |
| locations[4].source.is_in_doaj | False |
| locations[4].source.display_name | IslandScholar (University of Prince Edward Island) |
| locations[4].source.host_organization | https://openalex.org/I22188714 |
| locations[4].source.host_organization_name | University of Prince Edward Island |
| locations[4].source.host_organization_lineage | https://openalex.org/I22188714 |
| locations[4].license | cc-by |
| locations[4].pdf_url | |
| locations[4].version | submittedVersion |
| locations[4].raw_type | Journal Article |
| locations[4].license_id | https://openalex.org/licenses/cc-by |
| locations[4].is_accepted | False |
| locations[4].is_published | False |
| locations[4].raw_source_name | |
| locations[4].landing_page_url | https://www.frontiersin.org/article/10.3389/fmicb.2018.03351/full |
| locations[5].id | pmh:oai:pubmedcentral.nih.gov:6344404 |
| locations[5].is_oa | True |
| locations[5].source.id | https://openalex.org/S2764455111 |
| locations[5].source.issn | |
| locations[5].source.type | repository |
| locations[5].source.is_oa | False |
| locations[5].source.issn_l | |
| locations[5].source.is_core | False |
| locations[5].source.is_in_doaj | False |
| locations[5].source.display_name | PubMed Central |
| locations[5].source.host_organization | https://openalex.org/I1299303238 |
| locations[5].source.host_organization_name | National Institutes of Health |
| locations[5].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[5].license | cc-by |
| locations[5].pdf_url | |
| locations[5].version | submittedVersion |
| locations[5].raw_type | Text |
| locations[5].license_id | https://openalex.org/licenses/cc-by |
| locations[5].is_accepted | False |
| locations[5].is_published | False |
| locations[5].raw_source_name | Front Microbiol |
| locations[5].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/6344404 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5081638445 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-0649-289X |
| authorships[0].author.display_name | Libang Liang |
| authorships[0].countries | CA |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I22188714 |
| authorships[0].affiliations[0].raw_affiliation_string | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[0].institutions[0].id | https://openalex.org/I22188714 |
| authorships[0].institutions[0].ror | https://ror.org/02xh9x144 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I22188714 |
| authorships[0].institutions[0].country_code | CA |
| authorships[0].institutions[0].display_name | University of Prince Edward Island |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Libang Liang |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[1].author.id | https://openalex.org/A5058641273 |
| authorships[1].author.orcid | |
| authorships[1].author.display_name | Amanda Sproule |
| authorships[1].countries | CA |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I22188714 |
| authorships[1].affiliations[0].raw_affiliation_string | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[1].institutions[0].id | https://openalex.org/I22188714 |
| authorships[1].institutions[0].ror | https://ror.org/02xh9x144 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I22188714 |
| authorships[1].institutions[0].country_code | CA |
| authorships[1].institutions[0].display_name | University of Prince Edward Island |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Amanda Sproule |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[2].author.id | https://openalex.org/A5085511213 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-7220-8770 |
| authorships[2].author.display_name | Bradley Haltli |
| authorships[2].countries | CA |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I22188714 |
| authorships[2].affiliations[0].raw_affiliation_string | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[2].affiliations[1].institution_ids | https://openalex.org/I22188714 |
| authorships[2].affiliations[1].raw_affiliation_string | Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[2].affiliations[2].institution_ids | https://openalex.org/I4210107835 |
| authorships[2].affiliations[2].raw_affiliation_string | Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[2].institutions[0].id | https://openalex.org/I4210107835 |
| authorships[2].institutions[0].ror | https://ror.org/01rmpb303 |
| authorships[2].institutions[0].type | company |
| authorships[2].institutions[0].lineage | https://openalex.org/I4210107835 |
| authorships[2].institutions[0].country_code | CA |
| authorships[2].institutions[0].display_name | Nautilus Biosciences (Canada) |
| authorships[2].institutions[1].id | https://openalex.org/I22188714 |
| authorships[2].institutions[1].ror | https://ror.org/02xh9x144 |
| authorships[2].institutions[1].type | education |
| authorships[2].institutions[1].lineage | https://openalex.org/I22188714 |
| authorships[2].institutions[1].country_code | CA |
| authorships[2].institutions[1].display_name | University of Prince Edward Island |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Brad Haltli |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada, Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada, Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[3].author.id | https://openalex.org/A5047322923 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-6002-9257 |
| authorships[3].author.display_name | Douglas H. Marchbank |
| authorships[3].countries | CA |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I4210107835 |
| authorships[3].affiliations[0].raw_affiliation_string | Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[3].affiliations[1].institution_ids | https://openalex.org/I22188714 |
| authorships[3].affiliations[1].raw_affiliation_string | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[3].institutions[0].id | https://openalex.org/I4210107835 |
| authorships[3].institutions[0].ror | https://ror.org/01rmpb303 |
| authorships[3].institutions[0].type | company |
| authorships[3].institutions[0].lineage | https://openalex.org/I4210107835 |
| authorships[3].institutions[0].country_code | CA |
| authorships[3].institutions[0].display_name | Nautilus Biosciences (Canada) |
| authorships[3].institutions[1].id | https://openalex.org/I22188714 |
| authorships[3].institutions[1].ror | https://ror.org/02xh9x144 |
| authorships[3].institutions[1].type | education |
| authorships[3].institutions[1].lineage | https://openalex.org/I22188714 |
| authorships[3].institutions[1].country_code | CA |
| authorships[3].institutions[1].display_name | University of Prince Edward Island |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Douglas H. Marchbank |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada, Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[4].author.id | https://openalex.org/A5042254862 |
| authorships[4].author.orcid | https://orcid.org/0000-0003-2389-4388 |
| authorships[4].author.display_name | Fabrice Berrué |
| authorships[4].countries | CA |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I22188714 |
| authorships[4].affiliations[0].raw_affiliation_string | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[4].institutions[0].id | https://openalex.org/I22188714 |
| authorships[4].institutions[0].ror | https://ror.org/02xh9x144 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I22188714 |
| authorships[4].institutions[0].country_code | CA |
| authorships[4].institutions[0].display_name | University of Prince Edward Island |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Fabrice Berrué |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[5].author.id | https://openalex.org/A5002155410 |
| authorships[5].author.orcid | https://orcid.org/0000-0003-3107-4777 |
| authorships[5].author.display_name | David P. Overy |
| authorships[5].countries | CA |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I22188714 |
| authorships[5].affiliations[0].raw_affiliation_string | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[5].affiliations[1].institution_ids | https://openalex.org/I22188714 |
| authorships[5].affiliations[1].raw_affiliation_string | Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[5].institutions[0].id | https://openalex.org/I22188714 |
| authorships[5].institutions[0].ror | https://ror.org/02xh9x144 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I22188714 |
| authorships[5].institutions[0].country_code | CA |
| authorships[5].institutions[0].display_name | University of Prince Edward Island |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | David P. Overy |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada, Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[6].author.id | https://openalex.org/A5011616455 |
| authorships[6].author.orcid | |
| authorships[6].author.display_name | Kate McQuillan |
| authorships[6].countries | CA |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I4210107835 |
| authorships[6].affiliations[0].raw_affiliation_string | Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[6].institutions[0].id | https://openalex.org/I4210107835 |
| authorships[6].institutions[0].ror | https://ror.org/01rmpb303 |
| authorships[6].institutions[0].type | company |
| authorships[6].institutions[0].lineage | https://openalex.org/I4210107835 |
| authorships[6].institutions[0].country_code | CA |
| authorships[6].institutions[0].display_name | Nautilus Biosciences (Canada) |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Kate McQuillan |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[7].author.id | https://openalex.org/A5073538833 |
| authorships[7].author.orcid | |
| authorships[7].author.display_name | Martin Lanteigne |
| authorships[7].countries | CA |
| authorships[7].affiliations[0].institution_ids | https://openalex.org/I22188714 |
| authorships[7].affiliations[0].raw_affiliation_string | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[7].institutions[0].id | https://openalex.org/I22188714 |
| authorships[7].institutions[0].ror | https://ror.org/02xh9x144 |
| authorships[7].institutions[0].type | education |
| authorships[7].institutions[0].lineage | https://openalex.org/I22188714 |
| authorships[7].institutions[0].country_code | CA |
| authorships[7].institutions[0].display_name | University of Prince Edward Island |
| authorships[7].author_position | middle |
| authorships[7].raw_author_name | Martin Lanteigne |
| authorships[7].is_corresponding | False |
| authorships[7].raw_affiliation_strings | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[8].author.id | https://openalex.org/A5033965069 |
| authorships[8].author.orcid | |
| authorships[8].author.display_name | Noelle J. Duncan |
| authorships[8].countries | CA |
| authorships[8].affiliations[0].institution_ids | https://openalex.org/I4210107835 |
| authorships[8].affiliations[0].raw_affiliation_string | Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[8].institutions[0].id | https://openalex.org/I4210107835 |
| authorships[8].institutions[0].ror | https://ror.org/01rmpb303 |
| authorships[8].institutions[0].type | company |
| authorships[8].institutions[0].lineage | https://openalex.org/I4210107835 |
| authorships[8].institutions[0].country_code | CA |
| authorships[8].institutions[0].display_name | Nautilus Biosciences (Canada) |
| authorships[8].author_position | middle |
| authorships[8].raw_author_name | Noelle Duncan |
| authorships[8].is_corresponding | False |
| authorships[8].raw_affiliation_strings | Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[9].author.id | https://openalex.org/A5090645203 |
| authorships[9].author.orcid | https://orcid.org/0000-0003-4537-6573 |
| authorships[9].author.display_name | Hebelin Correa |
| authorships[9].countries | CA |
| authorships[9].affiliations[0].institution_ids | https://openalex.org/I4210107835 |
| authorships[9].affiliations[0].raw_affiliation_string | Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[9].institutions[0].id | https://openalex.org/I4210107835 |
| authorships[9].institutions[0].ror | https://ror.org/01rmpb303 |
| authorships[9].institutions[0].type | company |
| authorships[9].institutions[0].lineage | https://openalex.org/I4210107835 |
| authorships[9].institutions[0].country_code | CA |
| authorships[9].institutions[0].display_name | Nautilus Biosciences (Canada) |
| authorships[9].author_position | middle |
| authorships[9].raw_author_name | Hebelin Correa |
| authorships[9].is_corresponding | False |
| authorships[9].raw_affiliation_strings | Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[10].author.id | https://openalex.org/A5067050526 |
| authorships[10].author.orcid | https://orcid.org/0000-0002-2557-4321 |
| authorships[10].author.display_name | Russell G. Kerr |
| authorships[10].countries | CA |
| authorships[10].affiliations[0].institution_ids | https://openalex.org/I22188714 |
| authorships[10].affiliations[0].raw_affiliation_string | Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[10].affiliations[1].institution_ids | https://openalex.org/I22188714 |
| authorships[10].affiliations[1].raw_affiliation_string | Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada |
| authorships[10].affiliations[2].institution_ids | https://openalex.org/I4210107835 |
| authorships[10].affiliations[2].raw_affiliation_string | Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| authorships[10].institutions[0].id | https://openalex.org/I4210107835 |
| authorships[10].institutions[0].ror | https://ror.org/01rmpb303 |
| authorships[10].institutions[0].type | company |
| authorships[10].institutions[0].lineage | https://openalex.org/I4210107835 |
| authorships[10].institutions[0].country_code | CA |
| authorships[10].institutions[0].display_name | Nautilus Biosciences (Canada) |
| authorships[10].institutions[1].id | https://openalex.org/I22188714 |
| authorships[10].institutions[1].ror | https://ror.org/02xh9x144 |
| authorships[10].institutions[1].type | education |
| authorships[10].institutions[1].lineage | https://openalex.org/I22188714 |
| authorships[10].institutions[1].country_code | CA |
| authorships[10].institutions[1].display_name | University of Prince Edward Island |
| authorships[10].author_position | last |
| authorships[10].raw_author_name | Russell G. Kerr |
| authorships[10].is_corresponding | True |
| authorships[10].raw_affiliation_strings | Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE, Canada, Department of Chemistry, University of Prince Edward Island, Charlottetown, PE, Canada, Nautilus Biosciences Croda, Charlottetown, PE, Canada |
| has_content.pdf | False |
| has_content.grobid_xml | False |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://doi.org/10.3389/fmicb.2018.03351 |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Discovery of a New Natural Product and a Deactivation of a Quorum Sensing System by Culturing a “Producer” Bacterium With a Heat-Killed “Inducer” Culture |
| has_fulltext | False |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10593 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.9995999932289124 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1312 |
| primary_topic.subfield.display_name | Molecular Biology |
| primary_topic.display_name | Bacterial biofilms and quorum sensing |
| related_works | https://openalex.org/W3029497197, https://openalex.org/W1486285556, https://openalex.org/W2942672939, https://openalex.org/W2726936666, https://openalex.org/W2016497778, https://openalex.org/W4321436493, https://openalex.org/W2114076854, https://openalex.org/W3030741395, https://openalex.org/W2015718294, https://openalex.org/W2046127361 |
| cited_by_count | 18 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 2 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 3 |
| counts_by_year[2].year | 2023 |
| counts_by_year[2].cited_by_count | 4 |
| counts_by_year[3].year | 2022 |
| counts_by_year[3].cited_by_count | 2 |
| counts_by_year[4].year | 2021 |
| counts_by_year[4].cited_by_count | 2 |
| counts_by_year[5].year | 2020 |
| counts_by_year[5].cited_by_count | 3 |
| counts_by_year[6].year | 2019 |
| counts_by_year[6].cited_by_count | 2 |
| locations_count | 6 |
| best_oa_location.id | doi:10.3389/fmicb.2018.03351 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S2484704948 |
| best_oa_location.source.issn | 1664-302X |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 1664-302X |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | Frontiers in Microbiology |
| best_oa_location.source.host_organization | https://openalex.org/P4310320527 |
| best_oa_location.source.host_organization_name | Frontiers Media |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310320527 |
| best_oa_location.source.host_organization_lineage_names | Frontiers Media |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Frontiers in Microbiology |
| best_oa_location.landing_page_url | https://doi.org/10.3389/fmicb.2018.03351 |
| primary_location.id | doi:10.3389/fmicb.2018.03351 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S2484704948 |
| primary_location.source.issn | 1664-302X |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 1664-302X |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | Frontiers in Microbiology |
| primary_location.source.host_organization | https://openalex.org/P4310320527 |
| primary_location.source.host_organization_name | Frontiers Media |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310320527 |
| primary_location.source.host_organization_lineage_names | Frontiers Media |
| primary_location.license | cc-by |
| primary_location.pdf_url | |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Frontiers in Microbiology |
| primary_location.landing_page_url | https://doi.org/10.3389/fmicb.2018.03351 |
| publication_date | 2019-01-17 |
| publication_year | 2019 |
| referenced_works | https://openalex.org/W2165056189, https://openalex.org/W589727570, https://openalex.org/W2017343996, https://openalex.org/W2007055380, https://openalex.org/W2082255786, https://openalex.org/W1998348556, https://openalex.org/W2077665732, https://openalex.org/W2000945984, https://openalex.org/W2045316394, https://openalex.org/W2126793166, https://openalex.org/W1986165300, https://openalex.org/W2081170889, https://openalex.org/W2042754042, https://openalex.org/W1966195053, https://openalex.org/W2168687873, https://openalex.org/W1665661757, https://openalex.org/W2139954389, https://openalex.org/W2101078720, https://openalex.org/W2333489179, https://openalex.org/W1968085498, https://openalex.org/W2088755507, https://openalex.org/W2058128635, https://openalex.org/W110302458, https://openalex.org/W2224175912, https://openalex.org/W2009410432, https://openalex.org/W2164143799, https://openalex.org/W1964813583, https://openalex.org/W2328315760, https://openalex.org/W2061972317, https://openalex.org/W1991631485, https://openalex.org/W7056296537, https://openalex.org/W2029779699, https://openalex.org/W2824379583, https://openalex.org/W2010973183, https://openalex.org/W1969450113, https://openalex.org/W2159794839, https://openalex.org/W2021199154, https://openalex.org/W2069629231, https://openalex.org/W1971917234, https://openalex.org/W1980713037, https://openalex.org/W2032925644, https://openalex.org/W2097655674, https://openalex.org/W2013444724, https://openalex.org/W1977105094, https://openalex.org/W2074779228, https://openalex.org/W2064612941, https://openalex.org/W2132629607, https://openalex.org/W1989299822, https://openalex.org/W2088266211, https://openalex.org/W1996255717, https://openalex.org/W1991052542, https://openalex.org/W1856490997, https://openalex.org/W1988815096, https://openalex.org/W2015334134, https://openalex.org/W1518978916, https://openalex.org/W2111454395, https://openalex.org/W2058390510, https://openalex.org/W1985582040, https://openalex.org/W2021463054, https://openalex.org/W2162029553, https://openalex.org/W1980301095, https://openalex.org/W2153271862, https://openalex.org/W2026382449, https://openalex.org/W2013003830, https://openalex.org/W2323251859, https://openalex.org/W2290216457, https://openalex.org/W2090651792, https://openalex.org/W2151923418, https://openalex.org/W2000263727, https://openalex.org/W1969888221, https://openalex.org/W2436067889, https://openalex.org/W2555487210, https://openalex.org/W1827848435 |
| referenced_works_count | 73 |
| abstract_inverted_index.a | 3, 9, 54, 77, 117, 132 |
| abstract_inverted_index.28 | 36, 73 |
| abstract_inverted_index.D, | 76 |
| abstract_inverted_index.We | 141 |
| abstract_inverted_index.an | 113 |
| abstract_inverted_index.as | 8 |
| abstract_inverted_index.in | 41, 101 |
| abstract_inverted_index.is | 145 |
| abstract_inverted_index.of | 24, 44, 98, 110, 116, 119, 131, 135, 176 |
| abstract_inverted_index.to | 11, 67, 128, 147, 159, 166, 171 |
| abstract_inverted_index.we | 1, 34 |
| abstract_inverted_index.HHQ | 177 |
| abstract_inverted_index.PQS | 158 |
| abstract_inverted_index.and | 30, 60, 90, 123, 157, 178 |
| abstract_inverted_index.led | 127 |
| abstract_inverted_index.new | 13, 78, 103 |
| abstract_inverted_index.set | 109 |
| abstract_inverted_index.sp. | 88, 121 |
| abstract_inverted_index.the | 58, 85, 96, 99, 107, 129, 136, 149, 173 |
| abstract_inverted_index.via | 16 |
| abstract_inverted_index.was | 82, 164 |
| abstract_inverted_index.From | 106 |
| abstract_inverted_index.PQS. | 179 |
| abstract_inverted_index.This | 93 |
| abstract_inverted_index.able | 146 |
| abstract_inverted_index.each | 51, 68 |
| abstract_inverted_index.form | 160 |
| abstract_inverted_index.four | 31 |
| abstract_inverted_index.from | 57, 84 |
| abstract_inverted_index.have | 167 |
| abstract_inverted_index.pair | 86 |
| abstract_inverted_index.poor | 168 |
| abstract_inverted_index.same | 108 |
| abstract_inverted_index.some | 62 |
| abstract_inverted_index.that | 38, 50, 61, 143 |
| abstract_inverted_index.tool | 10 |
| abstract_inverted_index.were | 39, 65 |
| abstract_inverted_index.with | 21 |
| abstract_inverted_index.<i>P. | 150 |
| abstract_inverted_index.Among | 71 |
| abstract_inverted_index.PqsR, | 172 |
| abstract_inverted_index.Using | 27 |
| abstract_inverted_index.media | 20 |
| abstract_inverted_index.seven | 28 |
| abstract_inverted_index.shown | 165 |
| abstract_inverted_index.these | 72 |
| abstract_inverted_index.(HHQ), | 156 |
| abstract_inverted_index.(PQS). | 140 |
| abstract_inverted_index.Herein | 0 |
| abstract_inverted_index.analog | 134 |
| abstract_inverted_index.innate | 174 |
| abstract_inverted_index.quorum | 152 |
| abstract_inverted_index.result | 94 |
| abstract_inverted_index.signal | 139 |
| abstract_inverted_index.unique | 55 |
| abstract_inverted_index.binding | 169 |
| abstract_inverted_index.culture | 6 |
| abstract_inverted_index.induced | 40, 111 |
| abstract_inverted_index.inducer | 32, 52 |
| abstract_inverted_index.natural | 14, 80, 104 |
| abstract_inverted_index.product | 81 |
| abstract_inverted_index.sensing | 153 |
| abstract_inverted_index.Further, | 162 |
| abstract_inverted_index.PQS-GlcA | 163 |
| abstract_inverted_index.affinity | 170 |
| abstract_inverted_index.analysis | 43, 48 |
| abstract_inverted_index.chemical | 63 |
| abstract_inverted_index.cultures | 23 |
| abstract_inverted_index.describe | 2 |
| abstract_inverted_index.detected | 35 |
| abstract_inverted_index.discover | 12 |
| abstract_inverted_index.elicited | 53 |
| abstract_inverted_index.in-depth | 114 |
| abstract_inverted_index.isolated | 83 |
| abstract_inverted_index.modified | 4 |
| abstract_inverted_index.products | 15 |
| abstract_inverted_index.receptor | 175 |
| abstract_inverted_index.response | 56 |
| abstract_inverted_index.specific | 66 |
| abstract_inverted_index.strategy | 100 |
| abstract_inverted_index."inducer" | 25 |
| abstract_inverted_index.PQS-GlcA. | 161 |
| abstract_inverted_index.RKBH-B178 | 89, 122, 144 |
| abstract_inverted_index.bacterial | 5, 45 |
| abstract_inverted_index.discovery | 130 |
| abstract_inverted_index.indicated | 49 |
| abstract_inverted_index.microbes, | 33 |
| abstract_inverted_index.microbes. | 26 |
| abstract_inverted_index.products. | 105 |
| abstract_inverted_index.quinolone | 138 |
| abstract_inverted_index.responses | 64 |
| abstract_inverted_index.validated | 95 |
| abstract_inverted_index.autoclaved | 22, 124 |
| abstract_inverted_index.molecules, | 154 |
| abstract_inverted_index.Metabolomic | 47 |
| abstract_inverted_index.discovering | 102 |
| abstract_inverted_index.metabolites | 37 |
| abstract_inverted_index.methodology | 7 |
| abstract_inverted_index.pseudomonas | 137 |
| abstract_inverted_index.actinomycete | 18 |
| abstract_inverted_index.biotransform | 148 |
| abstract_inverted_index.combination. | 70 |
| abstract_inverted_index.demonstrated | 142 |
| abstract_inverted_index.fermentation | 19, 118 |
| abstract_inverted_index.metabolites, | 74, 112 |
| abstract_inverted_index.actinomycetes | 29, 59 |
| abstract_inverted_index.effectiveness | 97 |
| abstract_inverted_index.investigation | 115 |
| abstract_inverted_index.supplementing | 17 |
| abstract_inverted_index.<i>Pseudomonas | 125 |
| abstract_inverted_index.aeruginosa</i> | 126, 151 |
| abstract_inverted_index.fermentations. | 46 |
| abstract_inverted_index.glucuronidated | 133 |
| abstract_inverted_index.hydrazidomycin | 75 |
| abstract_inverted_index.smegmatis</i>. | 92 |
| abstract_inverted_index.<i>Mycobacterium | 91 |
| abstract_inverted_index.inducer-producer | 69 |
| abstract_inverted_index.<i>Streptomyces</i> | 87, 120 |
| abstract_inverted_index.UHPLC-HRESIMS-based | 42 |
| abstract_inverted_index.2-heptyl-4-quinolone | 155 |
| abstract_inverted_index.hydrazide-containing | 79 |
| cited_by_percentile_year.max | 97 |
| cited_by_percentile_year.min | 93 |
| corresponding_author_ids | https://openalex.org/A5067050526 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 11 |
| corresponding_institution_ids | https://openalex.org/I22188714, https://openalex.org/I4210107835 |
| citation_normalized_percentile.value | 0.71016332 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |