Distribution and associations for antimicrobial resistance and antibiotic resistance genes of Escherichia coli from musk deer (Moschus berezovskii) in Sichuan, China Article Swipe

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Tetracycline Biology Ampicillin Antibiotic resistance Escherichia coli Integron Microbiology Antimicrobial Chloramphenicol Antibiotics Gene Genetics

Hang Liu , Shulei Pan , Yuehong Cheng , Lijun Luo , Lei Zhou , Siping Fan , Liqin Wang , Shaoqi Jiang , Ziyao Zhou , Haifeng Liu , Shaqiu Zhang , Zhihua Ren , Xiaoping Ma , Cao SuiZhong , Liuhong Shen , Ya Wang , Dongjie Cai , Liping Gou , Yi Geng , Guangneng Peng , Qigui Yan , Yan Luo , Zhijun Zhong ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.1371/journal.pone.0289028 · OA: W4389043112

This study aimed to investigate the antimicrobial resistance (AMR), antibiotic resistance genes (ARGs) and integrons in 157 Escherichia coli ( E . coli ) strains isolated from feces of captive musk deer from 2 farms (Dujiang Yan and Barkam) in Sichuan province. Result showed that 91.72% (144/157) strains were resistant to at least one antimicrobial and 24.20% (38/157) strains were multi-drug resistant (MDR). The antibiotics that most E . coli strains were resistant to was sulfamethoxazole (85.99%), followed by ampicillin (26.11%) and tetracycline (24.84%). We further detected 13 ARGs in the 157 E . coli strains, of which bla TEM had the highest occurrence (91.72%), followed by aac(3’)-Iid (60.51%) and bla CTX-M (16.56%). Doxycycline, chloramphenicol, and ceftriaxone resistance were strongly correlated with the presence of tetB , floR and bla CTX-M , respectively. The strongest positive association among AMR phenotypes was ampicillin/cefuroxime sodium (OR, 828.000). The strongest positive association among 16 pairs of ARGs was sul1 / floR (OR, 21.667). Nine pairs positive associations were observed between AMR phenotypes and corresponding resistance genes and the strongest association was observed for CHL/ floR (OR, 301.167). Investigation of integrons revealed intl1 and intl2 genes were detected in 10.19% (16/157) and 1.27% (2/157) E . coli strains, respectively. Only one type of gene cassettes ( drA17-aadA5 ) was detected in class 1 integron positive strains. Our data implied musk deer is a reservoir of ARGs and positive associations were common observed among E . coli strains carrying AMRs and ARGs.