Dual-Emission Fluorescence Resonance Energy Transfer (FRET) PCR Discriminates Salmonella Pullorum and Gallinarum Article Swipe
Jiansen Gong
,
Nneka Vivian Iduu
,
Di Zhang
,
Kelly Chenoweth
,
Lanjing Wei
,
Yi Yang
,
Xinhong Dou
,
Chengming Wang
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.3390/microorganisms12091815
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.3390/microorganisms12091815
Salmonella Pullorum (S. Pullorum) and Salmonella Gallinarum (S. Gallinarum) are two biovars of Salmonella enterica serovar Gallinarum, responsible for pullorum disease and fowl typhoid, which are the most prevalent and pathogenic forms of salmonellosis in poultry in developing countries. Traditional differentiation methods for S. Pullorum and S. Gallinarum are based on distinct clinical manifestations and biochemical traits, given their indistinguishable nature via serological assays alone. Molecular differentiation methods such as allele-specific PCR and dual PCR combined with gel electrophoresis or enzyme digestion have also been used to discriminate S. Pullorum and S. Gallinarum, but the detection efficiency is not high. This investigation introduces a Fluorescence Resonance Energy Transfer (FRET) PCR assay targeting the pegB gene, exclusively found in specific Salmonella serovars such as S. Pullorum and S. Gallinarum, and exhibiting conserved single-nucleotide polymorphisms across these two biovars. High-resolution melting curve analysis demonstrates distinct dissolution profiles, facilitating the precise discrimination of S. Pullorum and S. Gallinarum. This FRET-PCR assay exhibits a detection limit of 10 copies per reaction and has been rigorously validated utilizing 17 reference strains and 39 clinical isolates. The innovation presented herein provides a valuable tool for the rapid differentiation of S. Pullorum and S. Gallinarum, thereby enhancing diagnostic efficiency and molecular surveillance of poultry Salmonella. The developed pegB-targeting FRET-PCR assay presents a promising alternative to current cumbersome and time-consuming diagnostic modalities, offering significant potential for expedited identification and control of Salmonella in poultry and mitigating economic losses associated with Salmonella contamination in poultry production.
Related Topics
Concepts
Biology
Salmonella
Salmonella enterica
Förster resonance energy transfer
Microbiology
High Resolution Melt
Serotype
Virology
Melting curve analysis
Polymerase chain reaction
Bacteria
Gene
Fluorescence
Genetics
Physics
Quantum mechanics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/microorganisms12091815
- OA Status
- gold
- Cited By
- 2
- References
- 21
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4402132152
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4402132152Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/microorganisms12091815Digital Object Identifier
- Title
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Dual-Emission Fluorescence Resonance Energy Transfer (FRET) PCR Discriminates Salmonella Pullorum and GallinarumWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-09-02Full publication date if available
- Authors
-
Jiansen Gong, Nneka Vivian Iduu, Di Zhang, Kelly Chenoweth, Lanjing Wei, Yi Yang, Xinhong Dou, Chengming WangList of authors in order
- Landing page
-
https://doi.org/10.3390/microorganisms12091815Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.3390/microorganisms12091815Direct OA link when available
- Concepts
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Biology, Salmonella, Salmonella enterica, Förster resonance energy transfer, Microbiology, High Resolution Melt, Serotype, Virology, Melting curve analysis, Polymerase chain reaction, Bacteria, Gene, Fluorescence, Genetics, Physics, Quantum mechanicsTop concepts (fields/topics) attached by OpenAlex
- Cited by
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2Total citation count in OpenAlex
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2025: 2Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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