Dual-view light-sheet imaging through a tilted glass interface using a deformable mirror Article Swipe
Nikita Vladimirov
,
Friedrich Preußer
,
Jan Wiśniewski
,
Ziv Yaniv
,
Ravi A. Desai
,
Andrew Woehler
,
Stephan Preibisch
·
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1364/boe.416737
YOU?
·
· 2021
· Open Access
·
· DOI: https://doi.org/10.1364/boe.416737
Light-sheet microscopy has become indispensable for imaging developing organisms, and imaging from multiple directions (views) is essential to improve its spatial resolution. We combine multi-view light-sheet microscopy with microfluidics using adaptive optics (deformable mirror) which corrects aberrations introduced by the 45 o -tilted glass coverslip. The optimal shape of the deformable mirror is computed by an iterative algorithm that optimizes the point-spread function in two orthogonal views. Simultaneous correction in two optical arms is achieved via a knife-edge mirror that splits the excitation path and combines the detection paths. Our design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experiments and high-content screening.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1364/boe.416737
- OA Status
- gold
- Cited By
- 14
- References
- 22
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3130047107
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W3130047107Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1364/boe.416737Digital Object Identifier
- Title
-
Dual-view light-sheet imaging through a tilted glass interface using a deformable mirrorWork title
- Type
-
articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2021Year of publication
- Publication date
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2021-02-16Full publication date if available
- Authors
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Nikita Vladimirov, Friedrich Preußer, Jan Wiśniewski, Ziv Yaniv, Ravi A. Desai, Andrew Woehler, Stephan PreibischList of authors in order
- Landing page
-
https://doi.org/10.1364/boe.416737Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.1364/boe.416737Direct OA link when available
- Concepts
-
Optics, Point spread function, Adaptive optics, Light sheet fluorescence microscopy, Microscopy, Deformable mirror, Microfluidics, Materials science, Microscope, Computer science, Computer vision, Physics, Nanotechnology, Scanning confocal electron microscopyTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
14Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 1, 2024: 4, 2023: 5, 2022: 3, 2021: 1Per-year citation counts (last 5 years)
- References (count)
-
22Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
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