Dual-view light-sheet imaging through tilted glass interface using a deformable mirror Article Swipe
Nikita Vladimirov
,
Friedrich Preußer
,
Jan Wiśniewski
,
Ziv Yaniv
,
RA Desai
,
Andrew Woehler
,
Stephan Preibisch
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1101/2020.10.20.345306
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1101/2020.10.20.345306
Light-sheet microscopy has become one of the primary tools for imaging live developing organisms because of its high speed, low phototoxicity, and optical sectioning capabilities. Detection from multiple sides (multi-view imaging) additionally allows nearly isotropic resolution via computational merging of the views. However, conventional light-sheet microscopes require that the sample is suspended in a gel to allow optical access from two or more sides. At the same time, the use of microfluidic devices is highly desirable for many experiments, but geometric constrains and strong optical aberrations caused by the coverslip titled relative to objectives make the use of multi-view lightsheet challenging for microfluidics. In this paper we describe the use of adaptive optics (AO) to enable multi-view light-sheet microscopy in such microfluidic setup by correcting optical aberrations introduced by the tilted coverslip. The optimal shape of deformable mirror is computed by an iterative stochastic gradient-descent algorithm that optimizes PSF in two orthogonal planes simultaneously. Simultaneous AO correction in two optical arms is achieved via a knife-edge mirror that splits excitation path and combines the detection path. We characterize the performance of this novel microscope setup and, by dual-view light-sheet imaging of C.elegans inside a microfluidic channel, demonstrate a drastic improvement of image quality due to AO and dual-view reconstruction. Our microscope design allows multi-view light-sheet microscopy with microfluidic devices for precisely controlled experimental conditions and high-content screening.
Related Topics
Concepts
Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1101/2020.10.20.345306
- https://www.biorxiv.org/content/biorxiv/early/2020/10/21/2020.10.20.345306.full.pdf
- OA Status
- green
- References
- 30
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W3093848865
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W3093848865Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1101/2020.10.20.345306Digital Object Identifier
- Title
-
Dual-view light-sheet imaging through tilted glass interface using a deformable mirrorWork title
- Type
-
preprintOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-10-21Full publication date if available
- Authors
-
Nikita Vladimirov, Friedrich Preußer, Jan Wiśniewski, Ziv Yaniv, RA Desai, Andrew Woehler, Stephan PreibischList of authors in order
- Landing page
-
https://doi.org/10.1101/2020.10.20.345306Publisher landing page
- PDF URL
-
https://www.biorxiv.org/content/biorxiv/early/2020/10/21/2020.10.20.345306.full.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://www.biorxiv.org/content/biorxiv/early/2020/10/21/2020.10.20.345306.full.pdfDirect OA link when available
- Concepts
-
Light sheet fluorescence microscopy, Optics, Microscope, Microfluidics, Microscopy, Optical microscope, Materials science, Adaptive optics, Optical path, Optical sectioning, Isotropy, Computer science, Physics, Nanotechnology, Scanning electron microscope, Scanning confocal electron microscopyTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
0Total citation count in OpenAlex
- References (count)
-
30Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W3093848865 |
|---|---|
| doi | https://doi.org/10.1101/2020.10.20.345306 |
| ids.doi | https://doi.org/10.1101/2020.10.20.345306 |
| ids.mag | 3093848865 |
| ids.openalex | https://openalex.org/W3093848865 |
| fwci | 0.0 |
| type | preprint |
| title | Dual-view light-sheet imaging through tilted glass interface using a deformable mirror |
| biblio.issue | |
| biblio.volume | |
| biblio.last_page | |
| biblio.first_page | |
| topics[0].id | https://openalex.org/T10540 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.9998999834060669 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1304 |
| topics[0].subfield.display_name | Biophysics |
| topics[0].display_name | Advanced Fluorescence Microscopy Techniques |
| topics[1].id | https://openalex.org/T11897 |
| topics[1].field.id | https://openalex.org/fields/31 |
| topics[1].field.display_name | Physics and Astronomy |
| topics[1].score | 0.9954000115394592 |
| topics[1].domain.id | https://openalex.org/domains/3 |
| topics[1].domain.display_name | Physical Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/3107 |
| topics[1].subfield.display_name | Atomic and Molecular Physics, and Optics |
| topics[1].display_name | Digital Holography and Microscopy |
| topics[2].id | https://openalex.org/T11569 |
| topics[2].field.id | https://openalex.org/fields/22 |
| topics[2].field.display_name | Engineering |
| topics[2].score | 0.992900013923645 |
| topics[2].domain.id | https://openalex.org/domains/3 |
| topics[2].domain.display_name | Physical Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2204 |
| topics[2].subfield.display_name | Biomedical Engineering |
| topics[2].display_name | Optical Coherence Tomography Applications |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C113976765 |
| concepts[0].level | 4 |
| concepts[0].score | 0.7032225728034973 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q1823502 |
| concepts[0].display_name | Light sheet fluorescence microscopy |
| concepts[1].id | https://openalex.org/C120665830 |
| concepts[1].level | 1 |
| concepts[1].score | 0.6811909675598145 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q14620 |
| concepts[1].display_name | Optics |
| concepts[2].id | https://openalex.org/C67649825 |
| concepts[2].level | 2 |
| concepts[2].score | 0.6507200002670288 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q196538 |
| concepts[2].display_name | Microscope |
| concepts[3].id | https://openalex.org/C8673954 |
| concepts[3].level | 2 |
| concepts[3].score | 0.6371020078659058 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q138845 |
| concepts[3].display_name | Microfluidics |
| concepts[4].id | https://openalex.org/C147080431 |
| concepts[4].level | 2 |
| concepts[4].score | 0.6274430751800537 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q1074953 |
| concepts[4].display_name | Microscopy |
| concepts[5].id | https://openalex.org/C77017923 |
| concepts[5].level | 3 |
| concepts[5].score | 0.5096280574798584 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q912313 |
| concepts[5].display_name | Optical microscope |
| concepts[6].id | https://openalex.org/C192562407 |
| concepts[6].level | 0 |
| concepts[6].score | 0.5056054592132568 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q228736 |
| concepts[6].display_name | Materials science |
| concepts[7].id | https://openalex.org/C132771110 |
| concepts[7].level | 2 |
| concepts[7].score | 0.49459096789360046 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q506922 |
| concepts[7].display_name | Adaptive optics |
| concepts[8].id | https://openalex.org/C31872934 |
| concepts[8].level | 2 |
| concepts[8].score | 0.4706622362136841 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q1417028 |
| concepts[8].display_name | Optical path |
| concepts[9].id | https://openalex.org/C20446124 |
| concepts[9].level | 3 |
| concepts[9].score | 0.4435345232486725 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q7098895 |
| concepts[9].display_name | Optical sectioning |
| concepts[10].id | https://openalex.org/C184050105 |
| concepts[10].level | 2 |
| concepts[10].score | 0.4349677562713623 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q273163 |
| concepts[10].display_name | Isotropy |
| concepts[11].id | https://openalex.org/C41008148 |
| concepts[11].level | 0 |
| concepts[11].score | 0.3543667495250702 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q21198 |
| concepts[11].display_name | Computer science |
| concepts[12].id | https://openalex.org/C121332964 |
| concepts[12].level | 0 |
| concepts[12].score | 0.27873873710632324 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q413 |
| concepts[12].display_name | Physics |
| concepts[13].id | https://openalex.org/C171250308 |
| concepts[13].level | 1 |
| concepts[13].score | 0.15720099210739136 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q11468 |
| concepts[13].display_name | Nanotechnology |
| concepts[14].id | https://openalex.org/C26771246 |
| concepts[14].level | 2 |
| concepts[14].score | 0.0 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q321095 |
| concepts[14].display_name | Scanning electron microscope |
| concepts[15].id | https://openalex.org/C187921700 |
| concepts[15].level | 3 |
| concepts[15].score | 0.0 |
| concepts[15].wikidata | https://www.wikidata.org/wiki/Q7430074 |
| concepts[15].display_name | Scanning confocal electron microscopy |
| keywords[0].id | https://openalex.org/keywords/light-sheet-fluorescence-microscopy |
| keywords[0].score | 0.7032225728034973 |
| keywords[0].display_name | Light sheet fluorescence microscopy |
| keywords[1].id | https://openalex.org/keywords/optics |
| keywords[1].score | 0.6811909675598145 |
| keywords[1].display_name | Optics |
| keywords[2].id | https://openalex.org/keywords/microscope |
| keywords[2].score | 0.6507200002670288 |
| keywords[2].display_name | Microscope |
| keywords[3].id | https://openalex.org/keywords/microfluidics |
| keywords[3].score | 0.6371020078659058 |
| keywords[3].display_name | Microfluidics |
| keywords[4].id | https://openalex.org/keywords/microscopy |
| keywords[4].score | 0.6274430751800537 |
| keywords[4].display_name | Microscopy |
| keywords[5].id | https://openalex.org/keywords/optical-microscope |
| keywords[5].score | 0.5096280574798584 |
| keywords[5].display_name | Optical microscope |
| keywords[6].id | https://openalex.org/keywords/materials-science |
| keywords[6].score | 0.5056054592132568 |
| keywords[6].display_name | Materials science |
| keywords[7].id | https://openalex.org/keywords/adaptive-optics |
| keywords[7].score | 0.49459096789360046 |
| keywords[7].display_name | Adaptive optics |
| keywords[8].id | https://openalex.org/keywords/optical-path |
| keywords[8].score | 0.4706622362136841 |
| keywords[8].display_name | Optical path |
| keywords[9].id | https://openalex.org/keywords/optical-sectioning |
| keywords[9].score | 0.4435345232486725 |
| keywords[9].display_name | Optical sectioning |
| keywords[10].id | https://openalex.org/keywords/isotropy |
| keywords[10].score | 0.4349677562713623 |
| keywords[10].display_name | Isotropy |
| keywords[11].id | https://openalex.org/keywords/computer-science |
| keywords[11].score | 0.3543667495250702 |
| keywords[11].display_name | Computer science |
| keywords[12].id | https://openalex.org/keywords/physics |
| keywords[12].score | 0.27873873710632324 |
| keywords[12].display_name | Physics |
| keywords[13].id | https://openalex.org/keywords/nanotechnology |
| keywords[13].score | 0.15720099210739136 |
| keywords[13].display_name | Nanotechnology |
| language | en |
| locations[0].id | doi:10.1101/2020.10.20.345306 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S4306402567 |
| locations[0].source.issn | |
| locations[0].source.type | repository |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | |
| locations[0].source.is_core | False |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| locations[0].source.host_organization | https://openalex.org/I2750212522 |
| locations[0].source.host_organization_name | Cold Spring Harbor Laboratory |
| locations[0].source.host_organization_lineage | https://openalex.org/I2750212522 |
| locations[0].license | cc-by-nc-nd |
| locations[0].pdf_url | https://www.biorxiv.org/content/biorxiv/early/2020/10/21/2020.10.20.345306.full.pdf |
| locations[0].version | acceptedVersion |
| locations[0].raw_type | posted-content |
| locations[0].license_id | https://openalex.org/licenses/cc-by-nc-nd |
| locations[0].is_accepted | True |
| locations[0].is_published | False |
| locations[0].raw_source_name | |
| locations[0].landing_page_url | https://doi.org/10.1101/2020.10.20.345306 |
| indexed_in | crossref |
| authorships[0].author.id | https://openalex.org/A5078478374 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-1159-0689 |
| authorships[0].author.display_name | Nikita Vladimirov |
| authorships[0].countries | DE |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I205582932 |
| authorships[0].affiliations[0].raw_affiliation_string | Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany |
| authorships[0].institutions[0].id | https://openalex.org/I205582932 |
| authorships[0].institutions[0].ror | https://ror.org/04p5ggc03 |
| authorships[0].institutions[0].type | facility |
| authorships[0].institutions[0].lineage | https://openalex.org/I1305996414, https://openalex.org/I205582932 |
| authorships[0].institutions[0].country_code | DE |
| authorships[0].institutions[0].display_name | Max Delbrück Center |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | N Vladimirov |
| authorships[0].is_corresponding | True |
| authorships[0].raw_affiliation_strings | Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany |
| authorships[1].author.id | https://openalex.org/A5020766717 |
| authorships[1].author.orcid | https://orcid.org/0000-0001-8231-2195 |
| authorships[1].author.display_name | Friedrich Preußer |
| authorships[1].countries | DE |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I205582932 |
| authorships[1].affiliations[0].raw_affiliation_string | Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany |
| authorships[1].institutions[0].id | https://openalex.org/I205582932 |
| authorships[1].institutions[0].ror | https://ror.org/04p5ggc03 |
| authorships[1].institutions[0].type | facility |
| authorships[1].institutions[0].lineage | https://openalex.org/I1305996414, https://openalex.org/I205582932 |
| authorships[1].institutions[0].country_code | DE |
| authorships[1].institutions[0].display_name | Max Delbrück Center |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | F Preusser |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany |
| authorships[2].author.id | https://openalex.org/A5067472504 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-9131-0711 |
| authorships[2].author.display_name | Jan Wiśniewski |
| authorships[2].countries | US |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I1299303238, https://openalex.org/I4210140884 |
| authorships[2].affiliations[0].raw_affiliation_string | Confocal Microscopy and Digital Imaging Facility, Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA |
| authorships[2].institutions[0].id | https://openalex.org/I4210140884 |
| authorships[2].institutions[0].ror | https://ror.org/040gcmg81 |
| authorships[2].institutions[0].type | government |
| authorships[2].institutions[0].lineage | https://openalex.org/I1299022934, https://openalex.org/I1299303238, https://openalex.org/I4210140884 |
| authorships[2].institutions[0].country_code | US |
| authorships[2].institutions[0].display_name | National Cancer Institute |
| authorships[2].institutions[1].id | https://openalex.org/I1299303238 |
| authorships[2].institutions[1].ror | https://ror.org/01cwqze88 |
| authorships[2].institutions[1].type | government |
| authorships[2].institutions[1].lineage | https://openalex.org/I1299022934, https://openalex.org/I1299303238 |
| authorships[2].institutions[1].country_code | US |
| authorships[2].institutions[1].display_name | National Institutes of Health |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | J Wisniewski |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Confocal Microscopy and Digital Imaging Facility, Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA |
| authorships[3].author.id | https://openalex.org/A5051027046 |
| authorships[3].author.orcid | https://orcid.org/0000-0003-0315-7727 |
| authorships[3].author.display_name | Ziv Yaniv |
| authorships[3].countries | US |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I1299303238, https://openalex.org/I4210134534 |
| authorships[3].affiliations[0].raw_affiliation_string | Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA |
| authorships[3].institutions[0].id | https://openalex.org/I4210134534 |
| authorships[3].institutions[0].ror | https://ror.org/043z4tv69 |
| authorships[3].institutions[0].type | facility |
| authorships[3].institutions[0].lineage | https://openalex.org/I1299022934, https://openalex.org/I1299303238, https://openalex.org/I4210134534 |
| authorships[3].institutions[0].country_code | US |
| authorships[3].institutions[0].display_name | National Institute of Allergy and Infectious Diseases |
| authorships[3].institutions[1].id | https://openalex.org/I1299303238 |
| authorships[3].institutions[1].ror | https://ror.org/01cwqze88 |
| authorships[3].institutions[1].type | government |
| authorships[3].institutions[1].lineage | https://openalex.org/I1299022934, https://openalex.org/I1299303238 |
| authorships[3].institutions[1].country_code | US |
| authorships[3].institutions[1].display_name | National Institutes of Health |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Z Yaniv |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA |
| authorships[4].author.id | https://openalex.org/A5109796520 |
| authorships[4].author.orcid | |
| authorships[4].author.display_name | RA Desai |
| authorships[4].countries | GB |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I2801081054 |
| authorships[4].affiliations[0].raw_affiliation_string | Francis Crick Institute, Making Science and Technology Platform, London NW1 1AT |
| authorships[4].institutions[0].id | https://openalex.org/I2801081054 |
| authorships[4].institutions[0].ror | https://ror.org/04tnbqb63 |
| authorships[4].institutions[0].type | facility |
| authorships[4].institutions[0].lineage | https://openalex.org/I2801081054 |
| authorships[4].institutions[0].country_code | GB |
| authorships[4].institutions[0].display_name | The Francis Crick Institute |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | RA Desai |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Francis Crick Institute, Making Science and Technology Platform, London NW1 1AT |
| authorships[5].author.id | https://openalex.org/A5001828442 |
| authorships[5].author.orcid | https://orcid.org/0000-0001-5157-9313 |
| authorships[5].author.display_name | Andrew Woehler |
| authorships[5].countries | DE |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I205582932 |
| authorships[5].affiliations[0].raw_affiliation_string | Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany |
| authorships[5].institutions[0].id | https://openalex.org/I205582932 |
| authorships[5].institutions[0].ror | https://ror.org/04p5ggc03 |
| authorships[5].institutions[0].type | facility |
| authorships[5].institutions[0].lineage | https://openalex.org/I1305996414, https://openalex.org/I205582932 |
| authorships[5].institutions[0].country_code | DE |
| authorships[5].institutions[0].display_name | Max Delbrück Center |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | A Woehler |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany |
| authorships[6].author.id | https://openalex.org/A5036369136 |
| authorships[6].author.orcid | https://orcid.org/0000-0002-0276-494X |
| authorships[6].author.display_name | Stephan Preibisch |
| authorships[6].countries | DE, US |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I195573530 |
| authorships[6].affiliations[0].raw_affiliation_string | HHMI Janelia Research Campus, Ashburn, Virginia, USA |
| authorships[6].affiliations[1].institution_ids | https://openalex.org/I205582932 |
| authorships[6].affiliations[1].raw_affiliation_string | Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany |
| authorships[6].institutions[0].id | https://openalex.org/I205582932 |
| authorships[6].institutions[0].ror | https://ror.org/04p5ggc03 |
| authorships[6].institutions[0].type | facility |
| authorships[6].institutions[0].lineage | https://openalex.org/I1305996414, https://openalex.org/I205582932 |
| authorships[6].institutions[0].country_code | DE |
| authorships[6].institutions[0].display_name | Max Delbrück Center |
| authorships[6].institutions[1].id | https://openalex.org/I195573530 |
| authorships[6].institutions[1].ror | https://ror.org/013sk6x84 |
| authorships[6].institutions[1].type | facility |
| authorships[6].institutions[1].lineage | https://openalex.org/I1344073410, https://openalex.org/I195573530 |
| authorships[6].institutions[1].country_code | US |
| authorships[6].institutions[1].display_name | Janelia Research Campus |
| authorships[6].author_position | last |
| authorships[6].raw_author_name | S Preibisch |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC), Berlin, Germany, HHMI Janelia Research Campus, Ashburn, Virginia, USA |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.biorxiv.org/content/biorxiv/early/2020/10/21/2020.10.20.345306.full.pdf |
| open_access.oa_status | green |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Dual-view light-sheet imaging through tilted glass interface using a deformable mirror |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10540 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.9998999834060669 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1304 |
| primary_topic.subfield.display_name | Biophysics |
| primary_topic.display_name | Advanced Fluorescence Microscopy Techniques |
| related_works | https://openalex.org/W1930341357, https://openalex.org/W4327555629, https://openalex.org/W2979590197, https://openalex.org/W115631233, https://openalex.org/W2098681947, https://openalex.org/W2950764594, https://openalex.org/W2343341536, https://openalex.org/W4297897531, https://openalex.org/W2323541887, https://openalex.org/W2793462084 |
| cited_by_count | 0 |
| locations_count | 1 |
| best_oa_location.id | doi:10.1101/2020.10.20.345306 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4306402567 |
| best_oa_location.source.issn | |
| best_oa_location.source.type | repository |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | |
| best_oa_location.source.is_core | False |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| best_oa_location.source.host_organization | https://openalex.org/I2750212522 |
| best_oa_location.source.host_organization_name | Cold Spring Harbor Laboratory |
| best_oa_location.source.host_organization_lineage | https://openalex.org/I2750212522 |
| best_oa_location.license | cc-by-nc-nd |
| best_oa_location.pdf_url | https://www.biorxiv.org/content/biorxiv/early/2020/10/21/2020.10.20.345306.full.pdf |
| best_oa_location.version | acceptedVersion |
| best_oa_location.raw_type | posted-content |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by-nc-nd |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | False |
| best_oa_location.raw_source_name | |
| best_oa_location.landing_page_url | https://doi.org/10.1101/2020.10.20.345306 |
| primary_location.id | doi:10.1101/2020.10.20.345306 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S4306402567 |
| primary_location.source.issn | |
| primary_location.source.type | repository |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | |
| primary_location.source.is_core | False |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | bioRxiv (Cold Spring Harbor Laboratory) |
| primary_location.source.host_organization | https://openalex.org/I2750212522 |
| primary_location.source.host_organization_name | Cold Spring Harbor Laboratory |
| primary_location.source.host_organization_lineage | https://openalex.org/I2750212522 |
| primary_location.license | cc-by-nc-nd |
| primary_location.pdf_url | https://www.biorxiv.org/content/biorxiv/early/2020/10/21/2020.10.20.345306.full.pdf |
| primary_location.version | acceptedVersion |
| primary_location.raw_type | posted-content |
| primary_location.license_id | https://openalex.org/licenses/cc-by-nc-nd |
| primary_location.is_accepted | True |
| primary_location.is_published | False |
| primary_location.raw_source_name | |
| primary_location.landing_page_url | https://doi.org/10.1101/2020.10.20.345306 |
| publication_date | 2020-10-21 |
| publication_year | 2020 |
| referenced_works | https://openalex.org/W2009622628, https://openalex.org/W2057132085, https://openalex.org/W2911525729, https://openalex.org/W2966422204, https://openalex.org/W2030224529, https://openalex.org/W2011301426, https://openalex.org/W2031312855, https://openalex.org/W2028911404, https://openalex.org/W2088909704, https://openalex.org/W2734339988, https://openalex.org/W2537691135, https://openalex.org/W2000558985, https://openalex.org/W2170608748, https://openalex.org/W1969393369, https://openalex.org/W3100274295, https://openalex.org/W2167279371, https://openalex.org/W2054823391, https://openalex.org/W2146292423, https://openalex.org/W3208652503, https://openalex.org/W2976039826, https://openalex.org/W2080348862, https://openalex.org/W2513309188, https://openalex.org/W2943569471, https://openalex.org/W3089242265, https://openalex.org/W2769556951, https://openalex.org/W3119820220, https://openalex.org/W2036002111, https://openalex.org/W3015275035, https://openalex.org/W3161639224, https://openalex.org/W2953583330 |
| referenced_works_count | 30 |
| abstract_inverted_index.a | 54, 165, 194, 198 |
| abstract_inverted_index.AO | 156, 206 |
| abstract_inverted_index.At | 65 |
| abstract_inverted_index.In | 104 |
| abstract_inverted_index.We | 177 |
| abstract_inverted_index.an | 142 |
| abstract_inverted_index.by | 88, 124, 129, 141, 187 |
| abstract_inverted_index.in | 53, 120, 150, 158 |
| abstract_inverted_index.is | 51, 74, 139, 162 |
| abstract_inverted_index.of | 6, 16, 40, 71, 98, 111, 136, 181, 191, 201 |
| abstract_inverted_index.or | 62 |
| abstract_inverted_index.to | 56, 93, 115, 205 |
| abstract_inverted_index.we | 107 |
| abstract_inverted_index.Our | 210 |
| abstract_inverted_index.PSF | 149 |
| abstract_inverted_index.The | 133 |
| abstract_inverted_index.and | 22, 83, 172, 207, 225 |
| abstract_inverted_index.but | 80 |
| abstract_inverted_index.due | 204 |
| abstract_inverted_index.for | 10, 77, 102, 220 |
| abstract_inverted_index.gel | 55 |
| abstract_inverted_index.has | 3 |
| abstract_inverted_index.its | 17 |
| abstract_inverted_index.low | 20 |
| abstract_inverted_index.one | 5 |
| abstract_inverted_index.the | 7, 41, 49, 66, 69, 89, 96, 109, 130, 174, 179 |
| abstract_inverted_index.two | 61, 151, 159 |
| abstract_inverted_index.use | 70, 97, 110 |
| abstract_inverted_index.via | 37, 164 |
| abstract_inverted_index.(AO) | 114 |
| abstract_inverted_index.and, | 186 |
| abstract_inverted_index.arms | 161 |
| abstract_inverted_index.from | 27, 60 |
| abstract_inverted_index.high | 18 |
| abstract_inverted_index.live | 12 |
| abstract_inverted_index.make | 95 |
| abstract_inverted_index.many | 78 |
| abstract_inverted_index.more | 63 |
| abstract_inverted_index.path | 171 |
| abstract_inverted_index.same | 67 |
| abstract_inverted_index.such | 121 |
| abstract_inverted_index.that | 48, 147, 168 |
| abstract_inverted_index.this | 105, 182 |
| abstract_inverted_index.with | 217 |
| abstract_inverted_index.allow | 57 |
| abstract_inverted_index.image | 202 |
| abstract_inverted_index.novel | 183 |
| abstract_inverted_index.paper | 106 |
| abstract_inverted_index.path. | 176 |
| abstract_inverted_index.setup | 123, 185 |
| abstract_inverted_index.shape | 135 |
| abstract_inverted_index.sides | 29 |
| abstract_inverted_index.time, | 68 |
| abstract_inverted_index.tools | 9 |
| abstract_inverted_index.access | 59 |
| abstract_inverted_index.allows | 33, 213 |
| abstract_inverted_index.become | 4 |
| abstract_inverted_index.caused | 87 |
| abstract_inverted_index.design | 212 |
| abstract_inverted_index.enable | 116 |
| abstract_inverted_index.highly | 75 |
| abstract_inverted_index.inside | 193 |
| abstract_inverted_index.mirror | 138, 167 |
| abstract_inverted_index.nearly | 34 |
| abstract_inverted_index.optics | 113 |
| abstract_inverted_index.planes | 153 |
| abstract_inverted_index.sample | 50 |
| abstract_inverted_index.sides. | 64 |
| abstract_inverted_index.speed, | 19 |
| abstract_inverted_index.splits | 169 |
| abstract_inverted_index.strong | 84 |
| abstract_inverted_index.tilted | 131 |
| abstract_inverted_index.titled | 91 |
| abstract_inverted_index.views. | 42 |
| abstract_inverted_index.because | 15 |
| abstract_inverted_index.devices | 73, 219 |
| abstract_inverted_index.drastic | 199 |
| abstract_inverted_index.imaging | 11, 190 |
| abstract_inverted_index.merging | 39 |
| abstract_inverted_index.optical | 23, 58, 85, 126, 160 |
| abstract_inverted_index.optimal | 134 |
| abstract_inverted_index.primary | 8 |
| abstract_inverted_index.quality | 203 |
| abstract_inverted_index.require | 47 |
| abstract_inverted_index.Abstract | 0 |
| abstract_inverted_index.However, | 43 |
| abstract_inverted_index.achieved | 163 |
| abstract_inverted_index.adaptive | 112 |
| abstract_inverted_index.channel, | 196 |
| abstract_inverted_index.combines | 173 |
| abstract_inverted_index.computed | 140 |
| abstract_inverted_index.describe | 108 |
| abstract_inverted_index.imaging) | 31 |
| abstract_inverted_index.multiple | 28 |
| abstract_inverted_index.relative | 92 |
| abstract_inverted_index.C.elegans | 192 |
| abstract_inverted_index.Detection | 26 |
| abstract_inverted_index.algorithm | 146 |
| abstract_inverted_index.coverslip | 90 |
| abstract_inverted_index.desirable | 76 |
| abstract_inverted_index.detection | 175 |
| abstract_inverted_index.dual-view | 188, 208 |
| abstract_inverted_index.geometric | 81 |
| abstract_inverted_index.isotropic | 35 |
| abstract_inverted_index.iterative | 143 |
| abstract_inverted_index.optimizes | 148 |
| abstract_inverted_index.organisms | 14 |
| abstract_inverted_index.precisely | 221 |
| abstract_inverted_index.suspended | 52 |
| abstract_inverted_index.conditions | 224 |
| abstract_inverted_index.constrains | 82 |
| abstract_inverted_index.controlled | 222 |
| abstract_inverted_index.correcting | 125 |
| abstract_inverted_index.correction | 157 |
| abstract_inverted_index.coverslip. | 132 |
| abstract_inverted_index.deformable | 137 |
| abstract_inverted_index.developing | 13 |
| abstract_inverted_index.excitation | 170 |
| abstract_inverted_index.introduced | 128 |
| abstract_inverted_index.knife-edge | 166 |
| abstract_inverted_index.lightsheet | 100 |
| abstract_inverted_index.microscope | 184, 211 |
| abstract_inverted_index.microscopy | 2, 119, 216 |
| abstract_inverted_index.multi-view | 99, 117, 214 |
| abstract_inverted_index.objectives | 94 |
| abstract_inverted_index.orthogonal | 152 |
| abstract_inverted_index.resolution | 36 |
| abstract_inverted_index.screening. | 227 |
| abstract_inverted_index.sectioning | 24 |
| abstract_inverted_index.stochastic | 144 |
| abstract_inverted_index.(multi-view | 30 |
| abstract_inverted_index.Light-sheet | 1 |
| abstract_inverted_index.aberrations | 86, 127 |
| abstract_inverted_index.challenging | 101 |
| abstract_inverted_index.demonstrate | 197 |
| abstract_inverted_index.improvement | 200 |
| abstract_inverted_index.light-sheet | 45, 118, 189, 215 |
| abstract_inverted_index.microscopes | 46 |
| abstract_inverted_index.performance | 180 |
| abstract_inverted_index.Simultaneous | 155 |
| abstract_inverted_index.additionally | 32 |
| abstract_inverted_index.characterize | 178 |
| abstract_inverted_index.conventional | 44 |
| abstract_inverted_index.experimental | 223 |
| abstract_inverted_index.experiments, | 79 |
| abstract_inverted_index.high-content | 226 |
| abstract_inverted_index.microfluidic | 72, 122, 195, 218 |
| abstract_inverted_index.capabilities. | 25 |
| abstract_inverted_index.computational | 38 |
| abstract_inverted_index.microfluidics. | 103 |
| abstract_inverted_index.phototoxicity, | 21 |
| abstract_inverted_index.reconstruction. | 209 |
| abstract_inverted_index.simultaneously. | 154 |
| abstract_inverted_index.gradient-descent | 145 |
| cited_by_percentile_year | |
| corresponding_author_ids | https://openalex.org/A5078478374 |
| countries_distinct_count | 3 |
| institutions_distinct_count | 7 |
| corresponding_institution_ids | https://openalex.org/I205582932 |
| sustainable_development_goals[0].id | https://metadata.un.org/sdg/9 |
| sustainable_development_goals[0].score | 0.41999998688697815 |
| sustainable_development_goals[0].display_name | Industry, innovation and infrastructure |
| citation_normalized_percentile.value | 0.07619588 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |