Dynamic contrast in scanning microscopic OCT Article Swipe
Michael Münter
,
Malte vom Endt
,
Mario Pieper
,
Malte Casper
,
Martin Ahrens
,
Tabea Kohlfaerber
,
Ramtin Rahmanzadeh
,
Peter König
,
Gereon Hüttmann
,
Hinnerk Schulz‐Hildebrandt
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1364/ol.396134
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1364/ol.396134
While optical coherence tomography (OCT) provides a resolution down to 1 µm, it has difficulties in visualizing cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast enhancement was demonstrated using time-domain full-field OCT (FF-OCT), which makes cellular and subcellular structures visible. The putative cause of the dynamic OCT signal is the site-dependent active motion of cellular structures in a sub-micrometer range, which provides histology-like contrast. Here we demonstrate dynamic contrast with a scanning frequency-domain OCT (FD-OCT), which we believe has crucial advantages. Given the inherent sectional imaging geometry, scanning FD-OCT provides depth-resolved images across tissue layers, a perspective known from histopathology, much faster and more efficiently than FF-OCT. Both shorter acquisition times and tomographic depth-sectioning reduce the sensitivity of dynamic contrast for bulk tissue motion artifacts and simplify their correction in post-processing. Dynamic contrast makes microscopic FD-OCT a promising tool for the histological analysis of unstained tissues.
Related Topics
Concepts
Optical coherence tomography
Contrast (vision)
Optics
Micrometer
Dynamic range
Dynamic contrast
Materials science
SIGNAL (programming language)
Resolution (logic)
Computer science
Biomedical engineering
Physics
Artificial intelligence
Magnetic resonance imaging
Medicine
Radiology
Programming language
Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.1364/ol.396134
- OA Status
- green
- Cited By
- 7
- References
- 27
- Related Works
- 20
- OpenAlex ID
- https://openalex.org/W3009003589
All OpenAlex metadata
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https://openalex.org/W3009003589Canonical identifier for this work in OpenAlex
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https://doi.org/10.1364/ol.396134Digital Object Identifier
- Title
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Dynamic contrast in scanning microscopic OCTWork title
- Type
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preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
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2020Year of publication
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2020-07-13Full publication date if available
- Authors
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Michael Münter, Malte vom Endt, Mario Pieper, Malte Casper, Martin Ahrens, Tabea Kohlfaerber, Ramtin Rahmanzadeh, Peter König, Gereon Hüttmann, Hinnerk Schulz‐HildebrandtList of authors in order
- Landing page
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https://doi.org/10.1364/ol.396134Publisher landing page
- Open access
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YesWhether a free full text is available
- OA status
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greenOpen access status per OpenAlex
- OA URL
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https://arxiv.org/pdf/2003.00006.pdfDirect OA link when available
- Concepts
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Optical coherence tomography, Contrast (vision), Optics, Micrometer, Dynamic range, Dynamic contrast, Materials science, SIGNAL (programming language), Resolution (logic), Computer science, Biomedical engineering, Physics, Artificial intelligence, Magnetic resonance imaging, Medicine, Radiology, Programming languageTop concepts (fields/topics) attached by OpenAlex
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7Total citation count in OpenAlex
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2024: 1, 2023: 2, 2021: 4Per-year citation counts (last 5 years)
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20Other works algorithmically related by OpenAlex
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