Dynamic spatiotemporal expression pattern of limbal stem cell putative biomarkers during mouse development Article Swipe
Zhi Hou Guo
,
Yiming Zeng
,
Jun Lin
·
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1016/j.exer.2020.107915
YOU?
·
· 2020
· Open Access
·
· DOI: https://doi.org/10.1016/j.exer.2020.107915
Limbal stem cells (LSCs), a subpopulation of limbal epithelial basal cells, are crucial to the homeostasis and wound healing of corneal epithelium. The identification and isolation of LSCs remains a challenge due to lack of specific LSCs biomarkers. In this study, Haematoxylin-eosin (HE), 4', 6-diamidino-2-phenylindole (DAPI), and immunohistochemistry (IHC) stains were performed on the pre- and post-natal limbus tissues of mice which has the advantage of more controllable in term of sampling age relative to human origin. By morphological analysis, we supported that there is an absence of the Palisades of Vogt (POV) in the mouse. The development of prenatal and neonatal cornea was dominated by its stroma, whereas after eyelids opened at P14, the corneal epithelial cells (CECs) quickly go stratification in response to the liquid-air interface. Based on IHC staining, we found that the expression of LSCs putative biomarkers in limbal epithelial basal cells appeared in chronological order as follows: Vim = p63 > CK14 > CK15 (where = represents same time; > represents earlier), and in corneal epithelial basal cells were weakened in chronological order as follows: Vim > p63 > CK15 > CK14, which might also represent the stemness degree. Furthermore, the dynamic spatial expression of the examined LSCs putative biomarkers during mouse development also implied a temporal restriction. The expression of Vim in epithelial cells of mouse ocular surface occurred during E12-E19 only. The expression of CK15 was completely undetectable in CECs after P14, whereas the others putative molecular markers of LSCs, such as p63 and CK14, still remained weak expression, suggesting that CK15 was suitable to serve as the mouse LSCs biomarkers after P14. In this study, our data demonstrated the dynamic spatiotemporal expression pattern of LSCs putative biomarkers in mouse was age-related and revealed the time spectrum of the expression of LSCs in mouse, which adds in our knowledge by understanding the dynamic expression pattern of biomarkers of stem cells relate to maintenance of their stemness.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.exer.2020.107915
- OA Status
- hybrid
- Cited By
- 11
- References
- 53
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2997923154
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- OpenAlex ID
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https://openalex.org/W2997923154Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.1016/j.exer.2020.107915Digital Object Identifier
- Title
-
Dynamic spatiotemporal expression pattern of limbal stem cell putative biomarkers during mouse developmentWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2020Year of publication
- Publication date
-
2020-01-05Full publication date if available
- Authors
-
Zhi Hou Guo, Yiming Zeng, Jun LinList of authors in order
- Landing page
-
https://doi.org/10.1016/j.exer.2020.107915Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
hybridOpen access status per OpenAlex
- OA URL
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https://doi.org/10.1016/j.exer.2020.107915Direct OA link when available
- Concepts
-
Biology, Stem cell, Cornea, Immunohistochemistry, Stroma, DAPI, Pathology, Basal (medicine), Epithelium, Corneal epithelium, Stem cell marker, Cell biology, Staining, Immunology, Medicine, Genetics, Endocrinology, Insulin, NeuroscienceTop concepts (fields/topics) attached by OpenAlex
- Cited by
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11Total citation count in OpenAlex
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2025: 4, 2024: 1, 2023: 2, 2022: 3, 2021: 1Per-year citation counts (last 5 years)
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53Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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