Effect of ultrasonic pretreatment on antifreeze activity of gluten antifreeze polypeptide Article Swipe
YOU?
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· 2024
· Open Access
·
· DOI: https://doi.org/10.1016/j.lwt.2024.116583
In this study, the effects of thermal hysteresis activity, ice recrystallization inhibition, amino acid composition and surface hydrophobicity of different ultrasonic pretreatments on gluten antifreeze polypeptide was studied. The results of the thermal hysteresis activity showed that the collaborative (flat-panel combined probe) ultrasonic working mode was superior to the single-frequence ultrasound mode. In the optimal working mode (15 min, 100 W/L), the results of polarized light microscope and gel chromatography column showed that ultrasonic pretreatment could effectively inhibit the growth of ice crystals, reduced the size of ice crystals, and to a certain extent, transfer the molecular weight distribution of peptides to small molecular peptides. The molecular weight was mainly concentrated at about 200–1000 u. The contents of Valine, Methionine, Leucine, Proline and Glutamic acid residues in the gluten antifreeze polypeptide pretreated by ultrasonic wave were relatively high and more hydrophobic groups were exposed. The secondary structure results showed that the molecular conformation of the polypeptide was mainly α-helix and β-sheet conformations. This paper systematically revealed the intrinsic correlation mechanism between the changes of key domains of substrate proteins and the antifreeze activity of enzymatic hydrolysis products assisted by ultrasoud, providing a theoretical basis for the preparation of antifreeze polypeptide.
Related Topics
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.1016/j.lwt.2024.116583
- OA Status
- gold
- Cited By
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- References
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- OpenAlex ID
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Raw OpenAlex JSON
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https://openalex.org/W4401275385Canonical identifier for this work in OpenAlex
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https://doi.org/10.1016/j.lwt.2024.116583Digital Object Identifier
- Title
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Effect of ultrasonic pretreatment on antifreeze activity of gluten antifreeze polypeptideWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2024Year of publication
- Publication date
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2024-08-01Full publication date if available
- Authors
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Yanyan Zhang, S.M. Wang, Na Xia, Penglei Guo, Yinli li, Hua Zhang, Suyun LiList of authors in order
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https://doi.org/10.1016/j.lwt.2024.116583Publisher landing page
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YesWhether a free full text is available
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goldOpen access status per OpenAlex
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https://doi.org/10.1016/j.lwt.2024.116583Direct OA link when available
- Concepts
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Antifreeze, Antifreeze protein, Gluten, Wheat gluten, Chemistry, Food science, Biochemistry, Organic chemistryTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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2025: 1Per-year citation counts (last 5 years)
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10Other works algorithmically related by OpenAlex
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| referenced_works | https://openalex.org/W4324135845, https://openalex.org/W1820772182, https://openalex.org/W2027368331, https://openalex.org/W2914937671, https://openalex.org/W4312204526, https://openalex.org/W4298395314, https://openalex.org/W2066570020, https://openalex.org/W1982388937, https://openalex.org/W4390198211, https://openalex.org/W2207709408, https://openalex.org/W1994812138, https://openalex.org/W2978984835, https://openalex.org/W2920804169, https://openalex.org/W3126087514, https://openalex.org/W4390531936, https://openalex.org/W1967400746, https://openalex.org/W4311765952, https://openalex.org/W1985301599, https://openalex.org/W3198060146, https://openalex.org/W4388766862, https://openalex.org/W2054013079, https://openalex.org/W1979946376, https://openalex.org/W3091693456, https://openalex.org/W2004867256, https://openalex.org/W1976519284, https://openalex.org/W2044183895, https://openalex.org/W2130799744, https://openalex.org/W2074415124, https://openalex.org/W4311145610, https://openalex.org/W2017898547, https://openalex.org/W4388845359, https://openalex.org/W4383497900, https://openalex.org/W2599719920, https://openalex.org/W2173179131, https://openalex.org/W4210762728, https://openalex.org/W2180507641, https://openalex.org/W4311679742, https://openalex.org/W2463543580, https://openalex.org/W4390543286, https://openalex.org/W2090157243, https://openalex.org/W4214743462 |
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| corresponding_author_ids | https://openalex.org/A5101764054, https://openalex.org/A5100408664 |
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