Ekspresi VEGF di Jaringan Hati Tikus Pasca Induksi 2AAF/CCl4 Article Swipe

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Isabella Kurnia Liem , Radietya Alvarabie , Fanny Oktorina , Firda Asma’ul Husna , Puspita Eka Wuyung , Ria Kodariah ·

YOU? · · 2023 · Open Access · · DOI: https://doi.org/10.23886/ejki.11.406.33-9

Vascular Endothelial Growth Factor (VEGF) merupakan sebuah faktor angiogenik poten yang terpicu ekspresinya jika jaringan hati mengalami cedera. Pada cedera hati kronis, VEGF diduga dapat berperan sebagai faktor pro-fibrotik atau anti-fibrotik. Penelitian ini dilakukan untuk mengetahui peningkatan ekspresi VEGF di jaringan hati tikus model dengan cedera hati kronis 2AAF/CCl4 (2-acetylaminofluorene dan carbon tetrachloride). Penelitian menggunakan desain eksperimental dan dilakukan sejak Oktober 2019- April 2021 di laboratorium Patologi Anatomi dan Departemen Anatomi Fakultas Kedokteran Universitas Indonesia. Dua belas ekor tikus Wistar dibagi menjadi dua kelompok, yaitu kelompok kontrol (normal; n=6) dan kelompok perlakuan (diinduksi dengan 2AAF/CCl4; n=6). Dilakukan pemeriksaan morfologi hati dengan pewarnaan hematoxylin eosin dan Masson’s trichrome dengan skoring NAFLD activity score (NAS) dan pengukuran kadar VEGF menggunakan pewarnaan imunohistokimia. Semua tikus kelompok perlakuan mengalami kerusakan hati dan fibrosis, dengan kerusakan hati terbanyak mencapai derajat 5 (5 dari 6) dan fibrosis hati terbanyak mencapai derajat 2 (4 dari 6). Sedangkan pada kelompok kontrol, semua tikus menunjukkan morfologi hati dalam kondisi normal (derajat 0). Skor ekspresi VEGF pada kelompok perlakuan (median [min-max] = (115 [97-141]) tidak meningkat bermakna (uji Mann-Whitney U; p=0,688) dibandingkan kelompok kontrol (112 [94-129]). Disimpulkan bahwa pada jaringan hati tikus model cedera hati yang diinduksi oleh 2AAF/CCl4 terdapat peningkatan VEGF namun tidak bermakna secara statistik.

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Medicine Gynecology Vascular endothelial growth factor Traditional medicine Gastroenterology Andrology Internal medicine VEGF receptors

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Type: article
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Landing Page: https://doi.org/10.23886/ejki.11.406.33-9
PDF: https://ejki.fk.ui.ac.id/index.php/journal/article/download/406/90
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DOI: https://doi.org/10.23886/ejki.11.406.33-9

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Title: Ekspresi VEGF di Jaringan Hati Tikus Pasca Induksi 2AAF/CCl4

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Publication year: 2023

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Publication date: 2023-05-31

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Authors: Isabella Kurnia Liem, Radietya Alvarabie, Fanny Oktorina, Firda Asma’ul Husna, Puspita Eka Wuyung, Ria Kodariah

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Landing page: https://doi.org/10.23886/ejki.11.406.33-9

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Concepts: Medicine, Gynecology, Vascular endothelial growth factor, Traditional medicine, Gastroenterology, Andrology, Internal medicine, VEGF receptors

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abstract_inverted_index.sebagai	26
abstract_inverted_index.skoring	108
abstract_inverted_index.terpicu	11
abstract_inverted_index.(derajat	161
abstract_inverted_index.(normal;	87
abstract_inverted_index.Fakultas	71
abstract_inverted_index.Patologi	66
abstract_inverted_index.Vascular	0
abstract_inverted_index.activity	110
abstract_inverted_index.bermakna	176, 204
abstract_inverted_index.berperan	25
abstract_inverted_index.ekspresi	37, 164
abstract_inverted_index.fibrosis	140
abstract_inverted_index.jaringan	14, 40, 189
abstract_inverted_index.kelompok	85, 90, 122, 151, 167, 182
abstract_inverted_index.kontrol,	152
abstract_inverted_index.mencapai	133, 143
abstract_inverted_index.p=0,688)	180
abstract_inverted_index.terdapat	199
abstract_inverted_index.2AAF/CCl4	48, 198
abstract_inverted_index.Dilakukan	96
abstract_inverted_index.Sedangkan	149
abstract_inverted_index.[97-141])	173
abstract_inverted_index.[min-max]	170
abstract_inverted_index.diinduksi	196
abstract_inverted_index.dilakukan	33, 58
abstract_inverted_index.fibrosis,	128
abstract_inverted_index.kelompok,	83
abstract_inverted_index.kerusakan	125, 130
abstract_inverted_index.mengalami	16, 124
abstract_inverted_index.meningkat	175
abstract_inverted_index.merupakan	5
abstract_inverted_index.morfologi	98, 156
abstract_inverted_index.perlakuan	91, 123, 168
abstract_inverted_index.pewarnaan	101, 118
abstract_inverted_index.terbanyak	132, 142
abstract_inverted_index.trichrome	106
abstract_inverted_index.(diinduksi	92
abstract_inverted_index.2AAF/CCl4;	94
abstract_inverted_index.Departemen	69
abstract_inverted_index.Indonesia.	74
abstract_inverted_index.Kedokteran	72
abstract_inverted_index.Masson’s	105
abstract_inverted_index.Penelitian	31, 53
abstract_inverted_index.[94-129]).	185
abstract_inverted_index.angiogenik	8
abstract_inverted_index.mengetahui	35
abstract_inverted_index.pengukuran	114
abstract_inverted_index.statistik.	206
abstract_inverted_index.Disimpulkan	186
abstract_inverted_index.Endothelial	1
abstract_inverted_index.Universitas	73
abstract_inverted_index.ekspresinya	12
abstract_inverted_index.hematoxylin	102
abstract_inverted_index.menggunakan	54, 117
abstract_inverted_index.menunjukkan	155
abstract_inverted_index.pemeriksaan	97
abstract_inverted_index.peningkatan	36, 200
abstract_inverted_index.Mann-Whitney	178
abstract_inverted_index.dibandingkan	181
abstract_inverted_index.laboratorium	65
abstract_inverted_index.pro-fibrotik	28
abstract_inverted_index.eksperimental	56
abstract_inverted_index.anti-fibrotik.	30
abstract_inverted_index.tetrachloride).	52
abstract_inverted_index.imunohistokimia.	119
abstract_inverted_index.(2-acetylaminofluorene	49
cited_by_percentile_year
countries_distinct_count	1
institutions_distinct_count	6
citation_normalized_percentile.value	0.03411731
citation_normalized_percentile.is_in_top_1_percent	False
citation_normalized_percentile.is_in_top_10_percent	False