Engineering and Purification of Microcin C7 Variants Resistant to Trypsin and Analysis of Their Biological Activity Article Swipe
Guangxin Yang
,
Lijun Shang
,
Lu Liu
,
Ze-qiang Li
,
Xiangfang Zeng
,
Xiuliang Ding
,
Jinxiu Huang
,
Shiyan Qiao
,
Haitao Yu
·
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/antibiotics12091346
YOU?
·
· 2023
· Open Access
·
· DOI: https://doi.org/10.3390/antibiotics12091346
Microcin C7 (McC) as a viable form of antimicrobial has gained substantial attention due to its distinctive antimicrobial activity, by targeting aspartyl tRNA synthetase. McC can be a potential solution against pathogenic microbial infections in the postantibiotic era. However, considering that degradation by digestive enzymes can disrupt the function of this peptide in the gastrointestinal tract, in this study, we attempt to design McC variants to overcome several barriers that may affect its stability and biological activity. The mccA gene encoding the McC peptide precursor was mutated and 12 new McC variants with trypsin resistance were found. The Yej+rimL− strain was used as an indicator to determine the minimum inhibitory concentrations (MICs). The results showed that three variants, including R2A, R2T and R2Q, among 12 variants formed by the replacement of the second arginine of the McC peptide with different amino acids, were resistant to trypsin and had an outstanding antimicrobial ability, with MIC values of 12.5, 25, and 25 μg/mL, respectively. Taken together, our findings show that the engineering of the site-directed mutagenesis of McC significantly enhances McC trypsin resistance and maintains a great antimicrobial activity.
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/antibiotics12091346
- https://www.mdpi.com/2079-6382/12/9/1346/pdf?version=1692679372
- OA Status
- gold
- Cited By
- 4
- References
- 25
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4386051062
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4386051062Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.3390/antibiotics12091346Digital Object Identifier
- Title
-
Engineering and Purification of Microcin C7 Variants Resistant to Trypsin and Analysis of Their Biological ActivityWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2023Year of publication
- Publication date
-
2023-08-22Full publication date if available
- Authors
-
Guangxin Yang, Lijun Shang, Lu Liu, Ze-qiang Li, Xiangfang Zeng, Xiuliang Ding, Jinxiu Huang, Shiyan Qiao, Haitao YuList of authors in order
- Landing page
-
https://doi.org/10.3390/antibiotics12091346Publisher landing page
- PDF URL
-
https://www.mdpi.com/2079-6382/12/9/1346/pdf?version=1692679372Direct link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
-
https://www.mdpi.com/2079-6382/12/9/1346/pdf?version=1692679372Direct OA link when available
- Concepts
-
Trypsin, Antimicrobial, Mutagenesis, Peptide, Enzyme, Biochemistry, Biology, Arginine, Antimicrobial peptides, Escherichia coli, Gene, Amino acid, Chemistry, Microbiology, MutantTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
4Total citation count in OpenAlex
- Citations by year (recent)
-
2025: 3, 2024: 1Per-year citation counts (last 5 years)
- References (count)
-
25Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4386051062 |
|---|---|
| doi | https://doi.org/10.3390/antibiotics12091346 |
| ids.doi | https://doi.org/10.3390/antibiotics12091346 |
| ids.pmid | https://pubmed.ncbi.nlm.nih.gov/37760643 |
| ids.openalex | https://openalex.org/W4386051062 |
| fwci | 0.74268163 |
| type | article |
| title | Engineering and Purification of Microcin C7 Variants Resistant to Trypsin and Analysis of Their Biological Activity |
| awards[0].id | https://openalex.org/G1830384233 |
| awards[0].funder_id | https://openalex.org/F4320335777 |
| awards[0].display_name | |
| awards[0].funder_award_id | 32030105 |
| awards[0].funder_display_name | National Key Research and Development Program of China |
| biblio.issue | 9 |
| biblio.volume | 12 |
| biblio.last_page | 1346 |
| biblio.first_page | 1346 |
| topics[0].id | https://openalex.org/T10521 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.9991999864578247 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1312 |
| topics[0].subfield.display_name | Molecular Biology |
| topics[0].display_name | RNA and protein synthesis mechanisms |
| topics[1].id | https://openalex.org/T10252 |
| topics[1].field.id | https://openalex.org/fields/27 |
| topics[1].field.display_name | Medicine |
| topics[1].score | 0.9977999925613403 |
| topics[1].domain.id | https://openalex.org/domains/4 |
| topics[1].domain.display_name | Health Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/2736 |
| topics[1].subfield.display_name | Pharmacology |
| topics[1].display_name | Microbial Natural Products and Biosynthesis |
| topics[2].id | https://openalex.org/T11103 |
| topics[2].field.id | https://openalex.org/fields/24 |
| topics[2].field.display_name | Immunology and Microbiology |
| topics[2].score | 0.9969000220298767 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/2404 |
| topics[2].subfield.display_name | Microbiology |
| topics[2].display_name | Antimicrobial Peptides and Activities |
| funders[0].id | https://openalex.org/F4320335777 |
| funders[0].ror | |
| funders[0].display_name | National Key Research and Development Program of China |
| is_xpac | False |
| apc_list.value | 2000 |
| apc_list.currency | CHF |
| apc_list.value_usd | 2165 |
| apc_paid.value | 2000 |
| apc_paid.currency | CHF |
| apc_paid.value_usd | 2165 |
| concepts[0].id | https://openalex.org/C2780340462 |
| concepts[0].level | 3 |
| concepts[0].score | 0.7791052460670471 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q127450 |
| concepts[0].display_name | Trypsin |
| concepts[1].id | https://openalex.org/C4937899 |
| concepts[1].level | 2 |
| concepts[1].score | 0.693952202796936 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q68541106 |
| concepts[1].display_name | Antimicrobial |
| concepts[2].id | https://openalex.org/C16318435 |
| concepts[2].level | 4 |
| concepts[2].score | 0.6099750399589539 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q149299 |
| concepts[2].display_name | Mutagenesis |
| concepts[3].id | https://openalex.org/C2779281246 |
| concepts[3].level | 2 |
| concepts[3].score | 0.5949183106422424 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q172847 |
| concepts[3].display_name | Peptide |
| concepts[4].id | https://openalex.org/C181199279 |
| concepts[4].level | 2 |
| concepts[4].score | 0.4755682349205017 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q8047 |
| concepts[4].display_name | Enzyme |
| concepts[5].id | https://openalex.org/C55493867 |
| concepts[5].level | 1 |
| concepts[5].score | 0.473073810338974 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q7094 |
| concepts[5].display_name | Biochemistry |
| concepts[6].id | https://openalex.org/C86803240 |
| concepts[6].level | 0 |
| concepts[6].score | 0.4659852385520935 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[6].display_name | Biology |
| concepts[7].id | https://openalex.org/C2777468819 |
| concepts[7].level | 3 |
| concepts[7].score | 0.46121442317962646 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q173670 |
| concepts[7].display_name | Arginine |
| concepts[8].id | https://openalex.org/C540938839 |
| concepts[8].level | 3 |
| concepts[8].score | 0.4528067111968994 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q1201508 |
| concepts[8].display_name | Antimicrobial peptides |
| concepts[9].id | https://openalex.org/C547475151 |
| concepts[9].level | 3 |
| concepts[9].score | 0.44091731309890747 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q25419 |
| concepts[9].display_name | Escherichia coli |
| concepts[10].id | https://openalex.org/C104317684 |
| concepts[10].level | 2 |
| concepts[10].score | 0.4240856468677521 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[10].display_name | Gene |
| concepts[11].id | https://openalex.org/C515207424 |
| concepts[11].level | 2 |
| concepts[11].score | 0.41254308819770813 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q8066 |
| concepts[11].display_name | Amino acid |
| concepts[12].id | https://openalex.org/C185592680 |
| concepts[12].level | 0 |
| concepts[12].score | 0.4124497175216675 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[12].display_name | Chemistry |
| concepts[13].id | https://openalex.org/C89423630 |
| concepts[13].level | 1 |
| concepts[13].score | 0.40744805335998535 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q7193 |
| concepts[13].display_name | Microbiology |
| concepts[14].id | https://openalex.org/C143065580 |
| concepts[14].level | 3 |
| concepts[14].score | 0.27529674768447876 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q3285695 |
| concepts[14].display_name | Mutant |
| keywords[0].id | https://openalex.org/keywords/trypsin |
| keywords[0].score | 0.7791052460670471 |
| keywords[0].display_name | Trypsin |
| keywords[1].id | https://openalex.org/keywords/antimicrobial |
| keywords[1].score | 0.693952202796936 |
| keywords[1].display_name | Antimicrobial |
| keywords[2].id | https://openalex.org/keywords/mutagenesis |
| keywords[2].score | 0.6099750399589539 |
| keywords[2].display_name | Mutagenesis |
| keywords[3].id | https://openalex.org/keywords/peptide |
| keywords[3].score | 0.5949183106422424 |
| keywords[3].display_name | Peptide |
| keywords[4].id | https://openalex.org/keywords/enzyme |
| keywords[4].score | 0.4755682349205017 |
| keywords[4].display_name | Enzyme |
| keywords[5].id | https://openalex.org/keywords/biochemistry |
| keywords[5].score | 0.473073810338974 |
| keywords[5].display_name | Biochemistry |
| keywords[6].id | https://openalex.org/keywords/biology |
| keywords[6].score | 0.4659852385520935 |
| keywords[6].display_name | Biology |
| keywords[7].id | https://openalex.org/keywords/arginine |
| keywords[7].score | 0.46121442317962646 |
| keywords[7].display_name | Arginine |
| keywords[8].id | https://openalex.org/keywords/antimicrobial-peptides |
| keywords[8].score | 0.4528067111968994 |
| keywords[8].display_name | Antimicrobial peptides |
| keywords[9].id | https://openalex.org/keywords/escherichia-coli |
| keywords[9].score | 0.44091731309890747 |
| keywords[9].display_name | Escherichia coli |
| keywords[10].id | https://openalex.org/keywords/gene |
| keywords[10].score | 0.4240856468677521 |
| keywords[10].display_name | Gene |
| keywords[11].id | https://openalex.org/keywords/amino-acid |
| keywords[11].score | 0.41254308819770813 |
| keywords[11].display_name | Amino acid |
| keywords[12].id | https://openalex.org/keywords/chemistry |
| keywords[12].score | 0.4124497175216675 |
| keywords[12].display_name | Chemistry |
| keywords[13].id | https://openalex.org/keywords/microbiology |
| keywords[13].score | 0.40744805335998535 |
| keywords[13].display_name | Microbiology |
| keywords[14].id | https://openalex.org/keywords/mutant |
| keywords[14].score | 0.27529674768447876 |
| keywords[14].display_name | Mutant |
| language | en |
| locations[0].id | doi:10.3390/antibiotics12091346 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S2736332253 |
| locations[0].source.issn | 2079-6382 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 2079-6382 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | True |
| locations[0].source.display_name | Antibiotics |
| locations[0].source.host_organization | https://openalex.org/P4310310987 |
| locations[0].source.host_organization_name | Multidisciplinary Digital Publishing Institute |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310310987 |
| locations[0].source.host_organization_lineage_names | Multidisciplinary Digital Publishing Institute |
| locations[0].license | cc-by |
| locations[0].pdf_url | https://www.mdpi.com/2079-6382/12/9/1346/pdf?version=1692679372 |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | https://openalex.org/licenses/cc-by |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Antibiotics |
| locations[0].landing_page_url | https://doi.org/10.3390/antibiotics12091346 |
| locations[1].id | pmid:37760643 |
| locations[1].is_oa | False |
| locations[1].source.id | https://openalex.org/S4306525036 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | PubMed |
| locations[1].source.host_organization | https://openalex.org/I1299303238 |
| locations[1].source.host_organization_name | National Institutes of Health |
| locations[1].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[1].license | |
| locations[1].pdf_url | |
| locations[1].version | publishedVersion |
| locations[1].raw_type | |
| locations[1].license_id | |
| locations[1].is_accepted | True |
| locations[1].is_published | True |
| locations[1].raw_source_name | Antibiotics (Basel, Switzerland) |
| locations[1].landing_page_url | https://pubmed.ncbi.nlm.nih.gov/37760643 |
| locations[2].id | pmh:oai:pubmedcentral.nih.gov:10525924 |
| locations[2].is_oa | True |
| locations[2].source.id | https://openalex.org/S2764455111 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | PubMed Central |
| locations[2].source.host_organization | https://openalex.org/I1299303238 |
| locations[2].source.host_organization_name | National Institutes of Health |
| locations[2].source.host_organization_lineage | https://openalex.org/I1299303238 |
| locations[2].license | cc-by |
| locations[2].pdf_url | https://pmc.ncbi.nlm.nih.gov/articles/PMC10525924/pdf/antibiotics-12-01346.pdf |
| locations[2].version | submittedVersion |
| locations[2].raw_type | Text |
| locations[2].license_id | https://openalex.org/licenses/cc-by |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | Antibiotics (Basel) |
| locations[2].landing_page_url | https://www.ncbi.nlm.nih.gov/pmc/articles/10525924 |
| locations[3].id | pmh:oai:doaj.org/article:34dd4d42a3974a7691eec7aeb13a0c70 |
| locations[3].is_oa | False |
| locations[3].source.id | https://openalex.org/S4306401280 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[3].source.host_organization | |
| locations[3].source.host_organization_name | |
| locations[3].license | |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | article |
| locations[3].license_id | |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | Antibiotics, Vol 12, Iss 9, p 1346 (2023) |
| locations[3].landing_page_url | https://doaj.org/article/34dd4d42a3974a7691eec7aeb13a0c70 |
| locations[4].id | pmh:oai:mdpi.com:/2079-6382/12/9/1346/ |
| locations[4].is_oa | True |
| locations[4].source.id | https://openalex.org/S4306400947 |
| locations[4].source.issn | |
| locations[4].source.type | repository |
| locations[4].source.is_oa | True |
| locations[4].source.issn_l | |
| locations[4].source.is_core | False |
| locations[4].source.is_in_doaj | False |
| locations[4].source.display_name | MDPI (MDPI AG) |
| locations[4].source.host_organization | https://openalex.org/I4210097602 |
| locations[4].source.host_organization_name | Multidisciplinary Digital Publishing Institute (Switzerland) |
| locations[4].source.host_organization_lineage | https://openalex.org/I4210097602 |
| locations[4].license | cc-by |
| locations[4].pdf_url | |
| locations[4].version | submittedVersion |
| locations[4].raw_type | Text |
| locations[4].license_id | https://openalex.org/licenses/cc-by |
| locations[4].is_accepted | False |
| locations[4].is_published | False |
| locations[4].raw_source_name | Antibiotics; Volume 12; Issue 9; Pages: 1346 |
| locations[4].landing_page_url | https://dx.doi.org/10.3390/antibiotics12091346 |
| indexed_in | crossref, doaj, pubmed |
| authorships[0].author.id | https://openalex.org/A5102896320 |
| authorships[0].author.orcid | |
| authorships[0].author.display_name | Guangxin Yang |
| authorships[0].countries | CN |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I4210149219, https://openalex.org/I52158045 |
| authorships[0].affiliations[0].raw_affiliation_string | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[0].institutions[0].id | https://openalex.org/I52158045 |
| authorships[0].institutions[0].ror | https://ror.org/04v3ywz14 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I52158045 |
| authorships[0].institutions[0].country_code | CN |
| authorships[0].institutions[0].display_name | China Agricultural University |
| authorships[0].institutions[1].id | https://openalex.org/I4210149219 |
| authorships[0].institutions[1].ror | https://ror.org/0490rfk07 |
| authorships[0].institutions[1].type | facility |
| authorships[0].institutions[1].lineage | https://openalex.org/I4210127390, https://openalex.org/I4210138501, https://openalex.org/I4210149219, https://openalex.org/I4210151987 |
| authorships[0].institutions[1].country_code | CN |
| authorships[0].institutions[1].display_name | Feed Research Institute |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Guangxin Yang |
| authorships[0].is_corresponding | False |
| authorships[0].raw_affiliation_strings | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[1].author.id | https://openalex.org/A5061052748 |
| authorships[1].author.orcid | https://orcid.org/0000-0002-4425-4809 |
| authorships[1].author.display_name | Lijun Shang |
| authorships[1].countries | CN |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I4210149219, https://openalex.org/I52158045 |
| authorships[1].affiliations[0].raw_affiliation_string | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[1].institutions[0].id | https://openalex.org/I52158045 |
| authorships[1].institutions[0].ror | https://ror.org/04v3ywz14 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I52158045 |
| authorships[1].institutions[0].country_code | CN |
| authorships[1].institutions[0].display_name | China Agricultural University |
| authorships[1].institutions[1].id | https://openalex.org/I4210149219 |
| authorships[1].institutions[1].ror | https://ror.org/0490rfk07 |
| authorships[1].institutions[1].type | facility |
| authorships[1].institutions[1].lineage | https://openalex.org/I4210127390, https://openalex.org/I4210138501, https://openalex.org/I4210149219, https://openalex.org/I4210151987 |
| authorships[1].institutions[1].country_code | CN |
| authorships[1].institutions[1].display_name | Feed Research Institute |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Lijun Shang |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[2].author.id | https://openalex.org/A5100396549 |
| authorships[2].author.orcid | https://orcid.org/0000-0003-2741-2542 |
| authorships[2].author.display_name | Lu Liu |
| authorships[2].countries | CN |
| authorships[2].affiliations[0].raw_affiliation_string | Luzhou Modern Agriculture Development Promotion Center, Luzhou 646000, China |
| authorships[2].affiliations[1].institution_ids | https://openalex.org/I4210149219, https://openalex.org/I52158045 |
| authorships[2].affiliations[1].raw_affiliation_string | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[2].institutions[0].id | https://openalex.org/I52158045 |
| authorships[2].institutions[0].ror | https://ror.org/04v3ywz14 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I52158045 |
| authorships[2].institutions[0].country_code | CN |
| authorships[2].institutions[0].display_name | China Agricultural University |
| authorships[2].institutions[1].id | https://openalex.org/I4210149219 |
| authorships[2].institutions[1].ror | https://ror.org/0490rfk07 |
| authorships[2].institutions[1].type | facility |
| authorships[2].institutions[1].lineage | https://openalex.org/I4210127390, https://openalex.org/I4210138501, https://openalex.org/I4210149219, https://openalex.org/I4210151987 |
| authorships[2].institutions[1].country_code | CN |
| authorships[2].institutions[1].display_name | Feed Research Institute |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Lu Liu |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Luzhou Modern Agriculture Development Promotion Center, Luzhou 646000, China, State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[3].author.id | https://openalex.org/A5106542688 |
| authorships[3].author.orcid | |
| authorships[3].author.display_name | Ze-qiang Li |
| authorships[3].affiliations[0].raw_affiliation_string | Shanghai Menon Animal Nutrition Technology Co., Ltd., Shanghai 201807, China |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Zeqiang Li |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Shanghai Menon Animal Nutrition Technology Co., Ltd., Shanghai 201807, China |
| authorships[4].author.id | https://openalex.org/A5001236643 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-0245-989X |
| authorships[4].author.display_name | Xiangfang Zeng |
| authorships[4].countries | CN |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I4210149219, https://openalex.org/I52158045 |
| authorships[4].affiliations[0].raw_affiliation_string | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[4].institutions[0].id | https://openalex.org/I52158045 |
| authorships[4].institutions[0].ror | https://ror.org/04v3ywz14 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I52158045 |
| authorships[4].institutions[0].country_code | CN |
| authorships[4].institutions[0].display_name | China Agricultural University |
| authorships[4].institutions[1].id | https://openalex.org/I4210149219 |
| authorships[4].institutions[1].ror | https://ror.org/0490rfk07 |
| authorships[4].institutions[1].type | facility |
| authorships[4].institutions[1].lineage | https://openalex.org/I4210127390, https://openalex.org/I4210138501, https://openalex.org/I4210149219, https://openalex.org/I4210151987 |
| authorships[4].institutions[1].country_code | CN |
| authorships[4].institutions[1].display_name | Feed Research Institute |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Xiangfang Zeng |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[5].author.id | https://openalex.org/A5064112418 |
| authorships[5].author.orcid | |
| authorships[5].author.display_name | Xiuliang Ding |
| authorships[5].countries | CN |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I4210124501 |
| authorships[5].affiliations[0].raw_affiliation_string | Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China |
| authorships[5].affiliations[1].raw_affiliation_string | National Center of Technology Innovation for Pigs, Rongchang, Chongqing 402460, China |
| authorships[5].institutions[0].id | https://openalex.org/I4210124501 |
| authorships[5].institutions[0].ror | https://ror.org/026mnhe80 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I4210124501 |
| authorships[5].institutions[0].country_code | CN |
| authorships[5].institutions[0].display_name | Chongqing Academy of Animal Science |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Xiuliang Ding |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China, National Center of Technology Innovation for Pigs, Rongchang, Chongqing 402460, China |
| authorships[6].author.id | https://openalex.org/A5031999679 |
| authorships[6].author.orcid | https://orcid.org/0000-0003-4114-3414 |
| authorships[6].author.display_name | Jinxiu Huang |
| authorships[6].countries | CN |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I4210124501 |
| authorships[6].affiliations[0].raw_affiliation_string | Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China |
| authorships[6].affiliations[1].raw_affiliation_string | National Center of Technology Innovation for Pigs, Rongchang, Chongqing 402460, China |
| authorships[6].institutions[0].id | https://openalex.org/I4210124501 |
| authorships[6].institutions[0].ror | https://ror.org/026mnhe80 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I4210124501 |
| authorships[6].institutions[0].country_code | CN |
| authorships[6].institutions[0].display_name | Chongqing Academy of Animal Science |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Jinxiu Huang |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Chongqing Academy of Animal Science, Rongchang, Chongqing 402460, China, National Center of Technology Innovation for Pigs, Rongchang, Chongqing 402460, China |
| authorships[7].author.id | https://openalex.org/A5111574196 |
| authorships[7].author.orcid | |
| authorships[7].author.display_name | Shiyan Qiao |
| authorships[7].countries | CN |
| authorships[7].affiliations[0].institution_ids | https://openalex.org/I4210149219, https://openalex.org/I52158045 |
| authorships[7].affiliations[0].raw_affiliation_string | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[7].institutions[0].id | https://openalex.org/I52158045 |
| authorships[7].institutions[0].ror | https://ror.org/04v3ywz14 |
| authorships[7].institutions[0].type | education |
| authorships[7].institutions[0].lineage | https://openalex.org/I52158045 |
| authorships[7].institutions[0].country_code | CN |
| authorships[7].institutions[0].display_name | China Agricultural University |
| authorships[7].institutions[1].id | https://openalex.org/I4210149219 |
| authorships[7].institutions[1].ror | https://ror.org/0490rfk07 |
| authorships[7].institutions[1].type | facility |
| authorships[7].institutions[1].lineage | https://openalex.org/I4210127390, https://openalex.org/I4210138501, https://openalex.org/I4210149219, https://openalex.org/I4210151987 |
| authorships[7].institutions[1].country_code | CN |
| authorships[7].institutions[1].display_name | Feed Research Institute |
| authorships[7].author_position | middle |
| authorships[7].raw_author_name | Shiyan Qiao |
| authorships[7].is_corresponding | False |
| authorships[7].raw_affiliation_strings | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[8].author.id | https://openalex.org/A5072832473 |
| authorships[8].author.orcid | https://orcid.org/0000-0002-7700-2392 |
| authorships[8].author.display_name | Haitao Yu |
| authorships[8].countries | CN |
| authorships[8].affiliations[0].institution_ids | https://openalex.org/I4210149219, https://openalex.org/I52158045 |
| authorships[8].affiliations[0].raw_affiliation_string | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| authorships[8].institutions[0].id | https://openalex.org/I52158045 |
| authorships[8].institutions[0].ror | https://ror.org/04v3ywz14 |
| authorships[8].institutions[0].type | education |
| authorships[8].institutions[0].lineage | https://openalex.org/I52158045 |
| authorships[8].institutions[0].country_code | CN |
| authorships[8].institutions[0].display_name | China Agricultural University |
| authorships[8].institutions[1].id | https://openalex.org/I4210149219 |
| authorships[8].institutions[1].ror | https://ror.org/0490rfk07 |
| authorships[8].institutions[1].type | facility |
| authorships[8].institutions[1].lineage | https://openalex.org/I4210127390, https://openalex.org/I4210138501, https://openalex.org/I4210149219, https://openalex.org/I4210151987 |
| authorships[8].institutions[1].country_code | CN |
| authorships[8].institutions[1].display_name | Feed Research Institute |
| authorships[8].author_position | last |
| authorships[8].raw_author_name | Haitao Yu |
| authorships[8].is_corresponding | True |
| authorships[8].raw_affiliation_strings | State Key Laboratory of Animal Nutrition and Feeding, Ministry of Agriculture Rural Affairs Feed Industry Centre, China Agricultural University, Beijing Bio-Feed Additives Key Laboratory, Beijing 100193, China |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://www.mdpi.com/2079-6382/12/9/1346/pdf?version=1692679372 |
| open_access.oa_status | gold |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Engineering and Purification of Microcin C7 Variants Resistant to Trypsin and Analysis of Their Biological Activity |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10521 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.9991999864578247 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1312 |
| primary_topic.subfield.display_name | Molecular Biology |
| primary_topic.display_name | RNA and protein synthesis mechanisms |
| related_works | https://openalex.org/W2011561549, https://openalex.org/W1971704222, https://openalex.org/W2025544676, https://openalex.org/W2318999255, https://openalex.org/W3189975923, https://openalex.org/W2011467532, https://openalex.org/W2356870418, https://openalex.org/W2038141943, https://openalex.org/W116306709, https://openalex.org/W2805213854 |
| cited_by_count | 4 |
| counts_by_year[0].year | 2025 |
| counts_by_year[0].cited_by_count | 3 |
| counts_by_year[1].year | 2024 |
| counts_by_year[1].cited_by_count | 1 |
| locations_count | 5 |
| best_oa_location.id | doi:10.3390/antibiotics12091346 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S2736332253 |
| best_oa_location.source.issn | 2079-6382 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 2079-6382 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | True |
| best_oa_location.source.display_name | Antibiotics |
| best_oa_location.source.host_organization | https://openalex.org/P4310310987 |
| best_oa_location.source.host_organization_name | Multidisciplinary Digital Publishing Institute |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310310987 |
| best_oa_location.source.host_organization_lineage_names | Multidisciplinary Digital Publishing Institute |
| best_oa_location.license | cc-by |
| best_oa_location.pdf_url | https://www.mdpi.com/2079-6382/12/9/1346/pdf?version=1692679372 |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | https://openalex.org/licenses/cc-by |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Antibiotics |
| best_oa_location.landing_page_url | https://doi.org/10.3390/antibiotics12091346 |
| primary_location.id | doi:10.3390/antibiotics12091346 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S2736332253 |
| primary_location.source.issn | 2079-6382 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 2079-6382 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | True |
| primary_location.source.display_name | Antibiotics |
| primary_location.source.host_organization | https://openalex.org/P4310310987 |
| primary_location.source.host_organization_name | Multidisciplinary Digital Publishing Institute |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310310987 |
| primary_location.source.host_organization_lineage_names | Multidisciplinary Digital Publishing Institute |
| primary_location.license | cc-by |
| primary_location.pdf_url | https://www.mdpi.com/2079-6382/12/9/1346/pdf?version=1692679372 |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | https://openalex.org/licenses/cc-by |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Antibiotics |
| primary_location.landing_page_url | https://doi.org/10.3390/antibiotics12091346 |
| publication_date | 2023-08-22 |
| publication_year | 2023 |
| referenced_works | https://openalex.org/W2757252374, https://openalex.org/W2973432546, https://openalex.org/W2969291231, https://openalex.org/W2115227691, https://openalex.org/W2140645633, https://openalex.org/W2028093584, https://openalex.org/W2070846320, https://openalex.org/W2149869218, https://openalex.org/W1981151557, https://openalex.org/W2100356537, https://openalex.org/W2048439515, https://openalex.org/W1544835748, https://openalex.org/W2052627468, https://openalex.org/W2159785306, https://openalex.org/W2122319025, https://openalex.org/W2153164798, https://openalex.org/W2025147903, https://openalex.org/W2912168115, https://openalex.org/W2070909432, https://openalex.org/W2064870983, https://openalex.org/W2048615721, https://openalex.org/W4213203364, https://openalex.org/W2095626429, https://openalex.org/W2044302426, https://openalex.org/W1564230599 |
| referenced_works_count | 25 |
| abstract_inverted_index.a | 4, 27, 183 |
| abstract_inverted_index.12 | 88, 124 |
| abstract_inverted_index.25 | 159 |
| abstract_inverted_index.C7 | 1 |
| abstract_inverted_index.an | 103, 148 |
| abstract_inverted_index.as | 3, 102 |
| abstract_inverted_index.be | 26 |
| abstract_inverted_index.by | 19, 42, 127 |
| abstract_inverted_index.in | 34, 52, 56 |
| abstract_inverted_index.of | 7, 49, 130, 134, 155, 170, 174 |
| abstract_inverted_index.to | 14, 61, 65, 105, 144 |
| abstract_inverted_index.we | 59 |
| abstract_inverted_index.25, | 157 |
| abstract_inverted_index.MIC | 153 |
| abstract_inverted_index.McC | 24, 63, 82, 90, 136, 175, 178 |
| abstract_inverted_index.R2T | 120 |
| abstract_inverted_index.The | 77, 97, 112 |
| abstract_inverted_index.and | 74, 87, 121, 146, 158, 181 |
| abstract_inverted_index.can | 25, 45 |
| abstract_inverted_index.due | 13 |
| abstract_inverted_index.had | 147 |
| abstract_inverted_index.has | 9 |
| abstract_inverted_index.its | 15, 72 |
| abstract_inverted_index.may | 70 |
| abstract_inverted_index.new | 89 |
| abstract_inverted_index.our | 164 |
| abstract_inverted_index.the | 35, 47, 53, 81, 107, 128, 131, 135, 168, 171 |
| abstract_inverted_index.was | 85, 100 |
| abstract_inverted_index.R2A, | 119 |
| abstract_inverted_index.R2Q, | 122 |
| abstract_inverted_index.era. | 37 |
| abstract_inverted_index.form | 6 |
| abstract_inverted_index.gene | 79 |
| abstract_inverted_index.mccA | 78 |
| abstract_inverted_index.show | 166 |
| abstract_inverted_index.tRNA | 22 |
| abstract_inverted_index.that | 40, 69, 115, 167 |
| abstract_inverted_index.this | 50, 57 |
| abstract_inverted_index.used | 101 |
| abstract_inverted_index.were | 95, 142 |
| abstract_inverted_index.with | 92, 138, 152 |
| abstract_inverted_index.(McC) | 2 |
| abstract_inverted_index.12.5, | 156 |
| abstract_inverted_index.Taken | 162 |
| abstract_inverted_index.amino | 140 |
| abstract_inverted_index.among | 123 |
| abstract_inverted_index.great | 184 |
| abstract_inverted_index.three | 116 |
| abstract_inverted_index.acids, | 141 |
| abstract_inverted_index.affect | 71 |
| abstract_inverted_index.design | 62 |
| abstract_inverted_index.formed | 126 |
| abstract_inverted_index.found. | 96 |
| abstract_inverted_index.gained | 10 |
| abstract_inverted_index.second | 132 |
| abstract_inverted_index.showed | 114 |
| abstract_inverted_index.strain | 99 |
| abstract_inverted_index.study, | 58 |
| abstract_inverted_index.tract, | 55 |
| abstract_inverted_index.values | 154 |
| abstract_inverted_index.viable | 5 |
| abstract_inverted_index.(MICs). | 111 |
| abstract_inverted_index.against | 30 |
| abstract_inverted_index.attempt | 60 |
| abstract_inverted_index.disrupt | 46 |
| abstract_inverted_index.enzymes | 44 |
| abstract_inverted_index.minimum | 108 |
| abstract_inverted_index.mutated | 86 |
| abstract_inverted_index.peptide | 51, 83, 137 |
| abstract_inverted_index.results | 113 |
| abstract_inverted_index.several | 67 |
| abstract_inverted_index.trypsin | 93, 145, 179 |
| abstract_inverted_index.μg/mL, | 160 |
| abstract_inverted_index.However, | 38 |
| abstract_inverted_index.Microcin | 0 |
| abstract_inverted_index.ability, | 151 |
| abstract_inverted_index.arginine | 133 |
| abstract_inverted_index.aspartyl | 21 |
| abstract_inverted_index.barriers | 68 |
| abstract_inverted_index.encoding | 80 |
| abstract_inverted_index.enhances | 177 |
| abstract_inverted_index.findings | 165 |
| abstract_inverted_index.function | 48 |
| abstract_inverted_index.overcome | 66 |
| abstract_inverted_index.solution | 29 |
| abstract_inverted_index.variants | 64, 91, 125 |
| abstract_inverted_index.activity, | 18 |
| abstract_inverted_index.activity. | 76, 186 |
| abstract_inverted_index.attention | 12 |
| abstract_inverted_index.determine | 106 |
| abstract_inverted_index.different | 139 |
| abstract_inverted_index.digestive | 43 |
| abstract_inverted_index.including | 118 |
| abstract_inverted_index.indicator | 104 |
| abstract_inverted_index.maintains | 182 |
| abstract_inverted_index.microbial | 32 |
| abstract_inverted_index.potential | 28 |
| abstract_inverted_index.precursor | 84 |
| abstract_inverted_index.resistant | 143 |
| abstract_inverted_index.stability | 73 |
| abstract_inverted_index.targeting | 20 |
| abstract_inverted_index.together, | 163 |
| abstract_inverted_index.variants, | 117 |
| abstract_inverted_index.biological | 75 |
| abstract_inverted_index.infections | 33 |
| abstract_inverted_index.inhibitory | 109 |
| abstract_inverted_index.pathogenic | 31 |
| abstract_inverted_index.resistance | 94, 180 |
| abstract_inverted_index.Yej+rimL− | 98 |
| abstract_inverted_index.considering | 39 |
| abstract_inverted_index.degradation | 41 |
| abstract_inverted_index.distinctive | 16 |
| abstract_inverted_index.engineering | 169 |
| abstract_inverted_index.mutagenesis | 173 |
| abstract_inverted_index.outstanding | 149 |
| abstract_inverted_index.replacement | 129 |
| abstract_inverted_index.substantial | 11 |
| abstract_inverted_index.synthetase. | 23 |
| abstract_inverted_index.antimicrobial | 8, 17, 150, 185 |
| abstract_inverted_index.respectively. | 161 |
| abstract_inverted_index.significantly | 176 |
| abstract_inverted_index.site-directed | 172 |
| abstract_inverted_index.concentrations | 110 |
| abstract_inverted_index.postantibiotic | 36 |
| abstract_inverted_index.gastrointestinal | 54 |
| cited_by_percentile_year.max | 97 |
| cited_by_percentile_year.min | 90 |
| corresponding_author_ids | https://openalex.org/A5072832473 |
| countries_distinct_count | 1 |
| institutions_distinct_count | 9 |
| corresponding_institution_ids | https://openalex.org/I4210149219, https://openalex.org/I52158045 |
| citation_normalized_percentile.value | 0.7308987 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |