Enhanced Antifungal Activity of Engineered Proteins via Swapping between Thioredoxin H2 and H3 Article Swipe
Jin‐Young Kim
,
Yong Hun
,
Il Ryong Kim
,
Heabin Kim
,
Ji Hyun Jung
,
Seong‐Cheol Park
,
Mi-Kyeong Jang
,
Sang Yeol Lee
,
Jung Ro Lee
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.3390/app9224766
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.3390/app9224766
Thioredoxins (Trxs) are proteins that act as antioxidants by facilitating the reduction of other proteins and are highly conserved in all organisms. Plant H-type Trx isoforms have different structures and perform multiple functions. Previous studies have reported that the low molecular weight AtTrx-H2 acts as a disulfide reductase and the high molecular weight AtTrx-H3 functions as an oxidoreductase and a molecular chaperone. In this study, we compared the antifungal activities of Arabidopsis Trx-H2 and -H3 with engineered proteins 2N3C and 3N2C via domain-swapping between the N- and C-terminal regions of Trx-H2 and -H3. All AtTrx-H variant proteins inhibited cell growth of various pathogenic fungal strains at pH 5.2 and pH 7.2 and showed significant intracellular accumulation in the fungal cells. Interestingly, only two engineered proteins penetrated the fungal cell wall and membrane, indicating their ability to destabilize the fungal cell membrane before internalization into the cytosol. To our knowledge, this is the first study that demonstrates novel functions of plant antioxidants AtTrx-H2 and -H3 as antifungal proteins and shows their enhanced activity using the domain swapping technique.
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- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3390/app9224766
- https://www.mdpi.com/2076-3417/9/22/4766/pdf?version=1573194256
- OA Status
- gold
- Cited By
- 1
- References
- 25
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2983709118
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Raw OpenAlex JSON
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https://openalex.org/W2983709118Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3390/app9224766Digital Object Identifier
- Title
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Enhanced Antifungal Activity of Engineered Proteins via Swapping between Thioredoxin H2 and H3Work title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2019Year of publication
- Publication date
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2019-11-08Full publication date if available
- Authors
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Jin‐Young Kim, Yong Hun, Il Ryong Kim, Heabin Kim, Ji Hyun Jung, Seong‐Cheol Park, Mi-Kyeong Jang, Sang Yeol Lee, Jung Ro LeeList of authors in order
- Landing page
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https://doi.org/10.3390/app9224766Publisher landing page
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https://www.mdpi.com/2076-3417/9/22/4766/pdf?version=1573194256Direct link to full text PDF
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goldOpen access status per OpenAlex
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https://www.mdpi.com/2076-3417/9/22/4766/pdf?version=1573194256Direct OA link when available
- Concepts
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Thioredoxin, Biochemistry, Intracellular, Cytosol, Chemistry, Cell biology, Internalization, Gene isoform, Arabidopsis, Biology, Cell, Enzyme, Gene, MutantTop concepts (fields/topics) attached by OpenAlex
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1Total citation count in OpenAlex
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10Other works algorithmically related by OpenAlex
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