Enhancement of Cellulase Production in Trichoderma reesei via Disruption of Multiple Protease Genes Identified by Comparative Secretomics Article Swipe
Yuanchao Qian
,
Lixia Zhong
,
Yu Sun
,
Ningning Sun
,
Lei Zhang
,
Weifeng Liu
,
Yinbo Qu
,
Yaohua Zhong
·
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2019.02784
YOU?
·
· 2019
· Open Access
·
· DOI: https://doi.org/10.3389/fmicb.2019.02784
The filamentous fungus Trichoderma reesei is one of the most studied cellulolytic organisms and the major producer of cellulases for industrial applications. However, undesired degradation of cellulases often happens in culture filtrates and commercial enzyme preparations. Even studies have been reported about describing proteolytic degradation of heterologous proteins in T. reesei, there are few systematic explorations concerning the extracellular proteases responsible for degradation of cellulases. In this study, the cellulase activity was observed to rapidly decrease at late cultivation stages using corn steep liquor (CSL) as the nitrogen source in T. reesei. It was discovered that this decrease may be caused by proteases. To identify the proteases, comparative secretomics was performed to analyze the concomitant proteases during the cellulase production. 12 candidate proteases from the secretome of T. reesei were identified and their encoding genes were individually deleted via homologous recombination. Furthermore, three target proteases (tre81070, tre120998, and tre123234) were simultaneously deleted by one-step genetic transformation. The triple deletion strain ΔP70 showed a 78% decrease in protease activity and a six-fold increase in cellulase activity at late fermentation stages. These results demonstrated the feasibility of improvement of cellulase production by genetically disrupting the potential protease genes to construct the T. reesei strains with low extracellular protease secretion. This dataset also provides an efficient approach for strain improvement by precise genetic engineering combined with "omics" strategy for high-production of industrial enzymes to reduce the cost of lignocellulose bioconversion.
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Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.3389/fmicb.2019.02784
- OA Status
- gold
- Cited By
- 79
- References
- 40
- Related Works
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- OpenAlex ID
- https://openalex.org/W2990443476
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W2990443476Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.3389/fmicb.2019.02784Digital Object Identifier
- Title
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Enhancement of Cellulase Production in Trichoderma reesei via Disruption of Multiple Protease Genes Identified by Comparative SecretomicsWork title
- Type
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articleOpenAlex work type
- Language
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enPrimary language
- Publication year
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2019Year of publication
- Publication date
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2019-12-03Full publication date if available
- Authors
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Yuanchao Qian, Lixia Zhong, Yu Sun, Ningning Sun, Lei Zhang, Weifeng Liu, Yinbo Qu, Yaohua ZhongList of authors in order
- Landing page
-
https://doi.org/10.3389/fmicb.2019.02784Publisher landing page
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YesWhether a free full text is available
- OA status
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goldOpen access status per OpenAlex
- OA URL
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https://doi.org/10.3389/fmicb.2019.02784Direct OA link when available
- Concepts
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Trichoderma reesei, Cellulase, Proteases, Protease, Biology, Biochemistry, Microbiology, EnzymeTop concepts (fields/topics) attached by OpenAlex
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79Total citation count in OpenAlex
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2025: 12, 2024: 14, 2023: 29, 2022: 5, 2021: 12Per-year citation counts (last 5 years)
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40Number of works referenced by this work
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10Other works algorithmically related by OpenAlex
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| primary_topic.display_name | Biofuel production and bioconversion |
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| counts_by_year[6].year | 2017 |
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