Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction Article Swipe
David M. Graham
,
Tomas Andersen
,
Lisa Sharek
,
Gunes Uzer
,
Katheryn E. Rothenberg
,
Brenton D. Hoffman
,
Janet Rubin
,
Martial Balland
,
James E. Bear
,
Keith Burridge
·
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.17615/qd12-gc03
YOU?
·
· 2025
· Open Access
·
· DOI: https://doi.org/10.17615/qd12-gc03
The nucleus has long been postulated to play a critical physical role during cell polarization and migration, but that role has not been defined or rigorously tested. Here, we enucleated cells to test the physical necessity of the nucleus during cell polarization and directed migration. Using enucleated mammalian cells (cytoplasts), we found that polarity establishment and cell migration in one dimension (1D) and two dimensions (2D) occur without the nucleus. Cytoplasts directionally migrate toward soluble (chemotaxis) and surface-bound (haptotaxis) extracellular cues and migrate collectively in scratch-wound assays. Consistent with previous studies, migration in 3D environments was dependent on the nucleus. In part, this likely reflects the decreased force exerted by cytoplasts on mechanically compliant substrates. This response is mimicked both in cells with nucleocytoskeletal defects and upon inhibition of actomyosin-based contractility. Together, our observations reveal that the nucleus is dispensable for polarization and migration in 1D and 2D but critical for proper cell mechanical responses.
Related Topics
Concepts
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.17615/qd12-gc03
- OA Status
- green
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4406597510
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W4406597510Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.17615/qd12-gc03Digital Object Identifier
- Title
-
Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransductionWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2025Year of publication
- Publication date
-
2025-01-17Full publication date if available
- Authors
-
David M. Graham, Tomas Andersen, Lisa Sharek, Gunes Uzer, Katheryn E. Rothenberg, Brenton D. Hoffman, Janet Rubin, Martial Balland, James E. Bear, Keith BurridgeList of authors in order
- Landing page
-
https://doi.org/10.17615/qd12-gc03Publisher landing page
- Open access
-
YesWhether a free full text is available
- OA status
-
greenOpen access status per OpenAlex
- OA URL
-
https://doi.org/10.17615/qd12-gc03Direct OA link when available
- Concepts
-
Mechanotransduction, Polarity (international relations), Nucleus, Differential (mechanical device), Cell biology, Neuroscience, Cell polarity, Cell, Biology, Chemistry, Physics, Genetics, ThermodynamicsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
0Total citation count in OpenAlex
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W4406597510 |
|---|---|
| doi | https://doi.org/10.17615/qd12-gc03 |
| ids.doi | https://doi.org/10.17615/qd12-gc03 |
| ids.openalex | https://openalex.org/W4406597510 |
| fwci | 0.0 |
| type | article |
| title | Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction |
| biblio.issue | |
| biblio.volume | |
| biblio.last_page | |
| biblio.first_page | |
| topics[0].id | https://openalex.org/T10492 |
| topics[0].field.id | https://openalex.org/fields/13 |
| topics[0].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[0].score | 0.901199996471405 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1307 |
| topics[0].subfield.display_name | Cell Biology |
| topics[0].display_name | Microtubule and mitosis dynamics |
| topics[1].id | https://openalex.org/T10379 |
| topics[1].field.id | https://openalex.org/fields/13 |
| topics[1].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[1].score | 0.9006999731063843 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1307 |
| topics[1].subfield.display_name | Cell Biology |
| topics[1].display_name | Cellular Mechanics and Interactions |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C181104049 |
| concepts[0].level | 2 |
| concepts[0].score | 0.8651553392410278 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q11936292 |
| concepts[0].display_name | Mechanotransduction |
| concepts[1].id | https://openalex.org/C2777361361 |
| concepts[1].level | 3 |
| concepts[1].score | 0.7823784351348877 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q1112585 |
| concepts[1].display_name | Polarity (international relations) |
| concepts[2].id | https://openalex.org/C2780723820 |
| concepts[2].level | 2 |
| concepts[2].score | 0.7161951661109924 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q1934178 |
| concepts[2].display_name | Nucleus |
| concepts[3].id | https://openalex.org/C93226319 |
| concepts[3].level | 2 |
| concepts[3].score | 0.6620562076568604 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q193137 |
| concepts[3].display_name | Differential (mechanical device) |
| concepts[4].id | https://openalex.org/C95444343 |
| concepts[4].level | 1 |
| concepts[4].score | 0.5362818241119385 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q7141 |
| concepts[4].display_name | Cell biology |
| concepts[5].id | https://openalex.org/C169760540 |
| concepts[5].level | 1 |
| concepts[5].score | 0.4706137776374817 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q207011 |
| concepts[5].display_name | Neuroscience |
| concepts[6].id | https://openalex.org/C49353375 |
| concepts[6].level | 3 |
| concepts[6].score | 0.4544232189655304 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q189100 |
| concepts[6].display_name | Cell polarity |
| concepts[7].id | https://openalex.org/C1491633281 |
| concepts[7].level | 2 |
| concepts[7].score | 0.38065195083618164 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q7868 |
| concepts[7].display_name | Cell |
| concepts[8].id | https://openalex.org/C86803240 |
| concepts[8].level | 0 |
| concepts[8].score | 0.34050190448760986 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[8].display_name | Biology |
| concepts[9].id | https://openalex.org/C185592680 |
| concepts[9].level | 0 |
| concepts[9].score | 0.3236042857170105 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q2329 |
| concepts[9].display_name | Chemistry |
| concepts[10].id | https://openalex.org/C121332964 |
| concepts[10].level | 0 |
| concepts[10].score | 0.18655192852020264 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q413 |
| concepts[10].display_name | Physics |
| concepts[11].id | https://openalex.org/C54355233 |
| concepts[11].level | 1 |
| concepts[11].score | 0.06512421369552612 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[11].display_name | Genetics |
| concepts[12].id | https://openalex.org/C97355855 |
| concepts[12].level | 1 |
| concepts[12].score | 0.0 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q11473 |
| concepts[12].display_name | Thermodynamics |
| keywords[0].id | https://openalex.org/keywords/mechanotransduction |
| keywords[0].score | 0.8651553392410278 |
| keywords[0].display_name | Mechanotransduction |
| keywords[1].id | https://openalex.org/keywords/polarity |
| keywords[1].score | 0.7823784351348877 |
| keywords[1].display_name | Polarity (international relations) |
| keywords[2].id | https://openalex.org/keywords/nucleus |
| keywords[2].score | 0.7161951661109924 |
| keywords[2].display_name | Nucleus |
| keywords[3].id | https://openalex.org/keywords/differential |
| keywords[3].score | 0.6620562076568604 |
| keywords[3].display_name | Differential (mechanical device) |
| keywords[4].id | https://openalex.org/keywords/cell-biology |
| keywords[4].score | 0.5362818241119385 |
| keywords[4].display_name | Cell biology |
| keywords[5].id | https://openalex.org/keywords/neuroscience |
| keywords[5].score | 0.4706137776374817 |
| keywords[5].display_name | Neuroscience |
| keywords[6].id | https://openalex.org/keywords/cell-polarity |
| keywords[6].score | 0.4544232189655304 |
| keywords[6].display_name | Cell polarity |
| keywords[7].id | https://openalex.org/keywords/cell |
| keywords[7].score | 0.38065195083618164 |
| keywords[7].display_name | Cell |
| keywords[8].id | https://openalex.org/keywords/biology |
| keywords[8].score | 0.34050190448760986 |
| keywords[8].display_name | Biology |
| keywords[9].id | https://openalex.org/keywords/chemistry |
| keywords[9].score | 0.3236042857170105 |
| keywords[9].display_name | Chemistry |
| keywords[10].id | https://openalex.org/keywords/physics |
| keywords[10].score | 0.18655192852020264 |
| keywords[10].display_name | Physics |
| keywords[11].id | https://openalex.org/keywords/genetics |
| keywords[11].score | 0.06512421369552612 |
| keywords[11].display_name | Genetics |
| language | en |
| locations[0].id | doi:10.17615/qd12-gc03 |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S7407051488 |
| locations[0].source.type | repository |
| locations[0].source.is_oa | False |
| locations[0].source.issn_l | |
| locations[0].source.is_core | False |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | UNC Libraries |
| locations[0].source.host_organization | |
| locations[0].source.host_organization_name | |
| locations[0].license | |
| locations[0].pdf_url | |
| locations[0].version | |
| locations[0].raw_type | article-journal |
| locations[0].license_id | |
| locations[0].is_accepted | False |
| locations[0].is_published | |
| locations[0].raw_source_name | |
| locations[0].landing_page_url | https://doi.org/10.17615/qd12-gc03 |
| indexed_in | datacite |
| authorships[0].author.id | https://openalex.org/A5042753300 |
| authorships[0].author.orcid | https://orcid.org/0000-0001-9868-5607 |
| authorships[0].author.display_name | David M. Graham |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Graham, David M. |
| authorships[0].is_corresponding | False |
| authorships[1].author.id | https://openalex.org/A5064252116 |
| authorships[1].author.orcid | https://orcid.org/0000-0003-4325-5399 |
| authorships[1].author.display_name | Tomas Andersen |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Andersen, Tomas |
| authorships[1].is_corresponding | False |
| authorships[2].author.id | https://openalex.org/A5011297717 |
| authorships[2].author.orcid | |
| authorships[2].author.display_name | Lisa Sharek |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Sharek, Lisa |
| authorships[2].is_corresponding | False |
| authorships[3].author.id | https://openalex.org/A5012529101 |
| authorships[3].author.orcid | https://orcid.org/0000-0002-1178-4942 |
| authorships[3].author.display_name | Gunes Uzer |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Uzer, Gunes |
| authorships[3].is_corresponding | False |
| authorships[4].author.id | https://openalex.org/A5075949337 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-8191-7528 |
| authorships[4].author.display_name | Katheryn E. Rothenberg |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Rothenberg, Katheryn |
| authorships[4].is_corresponding | False |
| authorships[5].author.id | https://openalex.org/A5017402732 |
| authorships[5].author.orcid | https://orcid.org/0000-0003-2296-1331 |
| authorships[5].author.display_name | Brenton D. Hoffman |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Hoffman, Brenton D. |
| authorships[5].is_corresponding | False |
| authorships[6].author.id | https://openalex.org/A5070581832 |
| authorships[6].author.orcid | https://orcid.org/0000-0003-3534-8667 |
| authorships[6].author.display_name | Janet Rubin |
| authorships[6].author_position | middle |
| authorships[6].raw_author_name | Rubin, Janet |
| authorships[6].is_corresponding | False |
| authorships[7].author.id | https://openalex.org/A5032169107 |
| authorships[7].author.orcid | https://orcid.org/0000-0002-6585-9735 |
| authorships[7].author.display_name | Martial Balland |
| authorships[7].author_position | middle |
| authorships[7].raw_author_name | Balland, Martial |
| authorships[7].is_corresponding | False |
| authorships[8].author.id | https://openalex.org/A5014769824 |
| authorships[8].author.orcid | https://orcid.org/0000-0002-8489-996X |
| authorships[8].author.display_name | James E. Bear |
| authorships[8].author_position | middle |
| authorships[8].raw_author_name | Bear, James E. |
| authorships[8].is_corresponding | False |
| authorships[9].author.id | https://openalex.org/A5070642099 |
| authorships[9].author.orcid | https://orcid.org/0000-0002-2152-9670 |
| authorships[9].author.display_name | Keith Burridge |
| authorships[9].author_position | last |
| authorships[9].raw_author_name | Burridge, Keith |
| authorships[9].is_corresponding | False |
| has_content.pdf | False |
| has_content.grobid_xml | False |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | https://doi.org/10.17615/qd12-gc03 |
| open_access.oa_status | green |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-01-20T00:00:00 |
| display_name | Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction |
| has_fulltext | False |
| is_retracted | False |
| updated_date | 2025-11-06T06:51:31.235846 |
| primary_topic.id | https://openalex.org/T10492 |
| primary_topic.field.id | https://openalex.org/fields/13 |
| primary_topic.field.display_name | Biochemistry, Genetics and Molecular Biology |
| primary_topic.score | 0.901199996471405 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1307 |
| primary_topic.subfield.display_name | Cell Biology |
| primary_topic.display_name | Microtubule and mitosis dynamics |
| related_works | https://openalex.org/W2141200120, https://openalex.org/W2029952727, https://openalex.org/W2046327341, https://openalex.org/W1994640080, https://openalex.org/W2128680169, https://openalex.org/W3032101389, https://openalex.org/W2082712598, https://openalex.org/W1966072954, https://openalex.org/W2979877637, https://openalex.org/W255972800 |
| cited_by_count | 0 |
| locations_count | 1 |
| best_oa_location.id | doi:10.17615/qd12-gc03 |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S7407051488 |
| best_oa_location.source.type | repository |
| best_oa_location.source.is_oa | False |
| best_oa_location.source.issn_l | |
| best_oa_location.source.is_core | False |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | UNC Libraries |
| best_oa_location.source.host_organization | |
| best_oa_location.source.host_organization_name | |
| best_oa_location.license | |
| best_oa_location.pdf_url | |
| best_oa_location.version | |
| best_oa_location.raw_type | article-journal |
| best_oa_location.license_id | |
| best_oa_location.is_accepted | False |
| best_oa_location.is_published | False |
| best_oa_location.raw_source_name | |
| best_oa_location.landing_page_url | https://doi.org/10.17615/qd12-gc03 |
| primary_location.id | doi:10.17615/qd12-gc03 |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S7407051488 |
| primary_location.source.type | repository |
| primary_location.source.is_oa | False |
| primary_location.source.issn_l | |
| primary_location.source.is_core | False |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | UNC Libraries |
| primary_location.source.host_organization | |
| primary_location.source.host_organization_name | |
| primary_location.license | |
| primary_location.pdf_url | |
| primary_location.version | |
| primary_location.raw_type | article-journal |
| primary_location.license_id | |
| primary_location.is_accepted | False |
| primary_location.is_published | False |
| primary_location.raw_source_name | |
| primary_location.landing_page_url | https://doi.org/10.17615/qd12-gc03 |
| publication_date | 2025-01-17 |
| publication_year | 2025 |
| referenced_works_count | 0 |
| abstract_inverted_index.a | 8 |
| abstract_inverted_index.1D | 145 |
| abstract_inverted_index.2D | 147 |
| abstract_inverted_index.3D | 93 |
| abstract_inverted_index.In | 100 |
| abstract_inverted_index.by | 109 |
| abstract_inverted_index.in | 58, 84, 92, 120, 144 |
| abstract_inverted_index.is | 117, 138 |
| abstract_inverted_index.of | 36, 128 |
| abstract_inverted_index.on | 97, 111 |
| abstract_inverted_index.or | 24 |
| abstract_inverted_index.to | 6, 31 |
| abstract_inverted_index.we | 28, 50 |
| abstract_inverted_index.The | 0 |
| abstract_inverted_index.and | 15, 42, 55, 62, 76, 81, 125, 142, 146 |
| abstract_inverted_index.but | 17, 148 |
| abstract_inverted_index.for | 140, 150 |
| abstract_inverted_index.has | 2, 20 |
| abstract_inverted_index.not | 21 |
| abstract_inverted_index.one | 59 |
| abstract_inverted_index.our | 132 |
| abstract_inverted_index.the | 33, 37, 68, 98, 105, 136 |
| abstract_inverted_index.two | 63 |
| abstract_inverted_index.was | 95 |
| abstract_inverted_index.(1D) | 61 |
| abstract_inverted_index.(2D) | 65 |
| abstract_inverted_index.This | 115 |
| abstract_inverted_index.been | 4, 22 |
| abstract_inverted_index.both | 119 |
| abstract_inverted_index.cell | 13, 40, 56, 152 |
| abstract_inverted_index.cues | 80 |
| abstract_inverted_index.long | 3 |
| abstract_inverted_index.play | 7 |
| abstract_inverted_index.role | 11, 19 |
| abstract_inverted_index.test | 32 |
| abstract_inverted_index.that | 18, 52, 135 |
| abstract_inverted_index.this | 102 |
| abstract_inverted_index.upon | 126 |
| abstract_inverted_index.with | 88, 122 |
| abstract_inverted_index.Here, | 27 |
| abstract_inverted_index.Using | 45 |
| abstract_inverted_index.cells | 30, 48, 121 |
| abstract_inverted_index.force | 107 |
| abstract_inverted_index.found | 51 |
| abstract_inverted_index.occur | 66 |
| abstract_inverted_index.part, | 101 |
| abstract_inverted_index.during | 12, 39 |
| abstract_inverted_index.likely | 103 |
| abstract_inverted_index.proper | 151 |
| abstract_inverted_index.reveal | 134 |
| abstract_inverted_index.toward | 73 |
| abstract_inverted_index.assays. | 86 |
| abstract_inverted_index.defects | 124 |
| abstract_inverted_index.defined | 23 |
| abstract_inverted_index.exerted | 108 |
| abstract_inverted_index.migrate | 72, 82 |
| abstract_inverted_index.nucleus | 1, 38, 137 |
| abstract_inverted_index.soluble | 74 |
| abstract_inverted_index.tested. | 26 |
| abstract_inverted_index.without | 67 |
| abstract_inverted_index.critical | 9, 149 |
| abstract_inverted_index.directed | 43 |
| abstract_inverted_index.mimicked | 118 |
| abstract_inverted_index.nucleus. | 69, 99 |
| abstract_inverted_index.physical | 10, 34 |
| abstract_inverted_index.polarity | 53 |
| abstract_inverted_index.previous | 89 |
| abstract_inverted_index.reflects | 104 |
| abstract_inverted_index.response | 116 |
| abstract_inverted_index.studies, | 90 |
| abstract_inverted_index.Together, | 131 |
| abstract_inverted_index.compliant | 113 |
| abstract_inverted_index.decreased | 106 |
| abstract_inverted_index.dependent | 96 |
| abstract_inverted_index.dimension | 60 |
| abstract_inverted_index.mammalian | 47 |
| abstract_inverted_index.migration | 57, 91, 143 |
| abstract_inverted_index.necessity | 35 |
| abstract_inverted_index.Consistent | 87 |
| abstract_inverted_index.Cytoplasts | 70 |
| abstract_inverted_index.cytoplasts | 110 |
| abstract_inverted_index.dimensions | 64 |
| abstract_inverted_index.enucleated | 29, 46 |
| abstract_inverted_index.inhibition | 127 |
| abstract_inverted_index.mechanical | 153 |
| abstract_inverted_index.migration, | 16 |
| abstract_inverted_index.migration. | 44 |
| abstract_inverted_index.postulated | 5 |
| abstract_inverted_index.responses. | 154 |
| abstract_inverted_index.rigorously | 25 |
| abstract_inverted_index.dispensable | 139 |
| abstract_inverted_index.substrates. | 114 |
| abstract_inverted_index.(chemotaxis) | 75 |
| abstract_inverted_index.(haptotaxis) | 78 |
| abstract_inverted_index.collectively | 83 |
| abstract_inverted_index.environments | 94 |
| abstract_inverted_index.mechanically | 112 |
| abstract_inverted_index.observations | 133 |
| abstract_inverted_index.polarization | 14, 41, 141 |
| abstract_inverted_index.(cytoplasts), | 49 |
| abstract_inverted_index.directionally | 71 |
| abstract_inverted_index.establishment | 54 |
| abstract_inverted_index.extracellular | 79 |
| abstract_inverted_index.scratch-wound | 85 |
| abstract_inverted_index.surface-bound | 77 |
| abstract_inverted_index.contractility. | 130 |
| abstract_inverted_index.actomyosin-based | 129 |
| abstract_inverted_index.nucleocytoskeletal | 123 |
| cited_by_percentile_year | |
| countries_distinct_count | 0 |
| institutions_distinct_count | 10 |
| citation_normalized_percentile.value | 0.01637877 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |