Establishment and in-house validation of stem-loop RT PCR method for MicroRNA398 expression analysis Article Swipe
Gordana Timotijević
,
Mira Milisavljević
,
Dragana Nikolić
,
Boško Milovanović
,
Dragana Nikolić
,
Miroslav Nikolić
,
Jelena Samardžić
·
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.2298/gensr1502405t
YOU?
·
· 2015
· Open Access
·
· DOI: https://doi.org/10.2298/gensr1502405t
MicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by ?stem-loop? primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for ?stem loop? primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as ?stem loop? RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.
Related Topics
Concepts
microRNA
Biology
Economic shortage
Computational biology
Gene expression
Gene
Gene expression profiling
Stem-loop
Transcription factor
Real-time polymerase chain reaction
Cell biology
Genetics
RNA
Philosophy
Government (linguistics)
Linguistics
Metadata
- Type
- article
- Language
- en
- Landing Page
- https://doi.org/10.2298/gensr1502405t
- http://www.doiserbia.nb.rs/ft.aspx?id=0534-00121502405T
- OA Status
- diamond
- References
- 17
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W2301058572
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
-
https://openalex.org/W2301058572Canonical identifier for this work in OpenAlex
- DOI
-
https://doi.org/10.2298/gensr1502405tDigital Object Identifier
- Title
-
Establishment and in-house validation of stem-loop RT PCR method for MicroRNA398 expression analysisWork title
- Type
-
articleOpenAlex work type
- Language
-
enPrimary language
- Publication year
-
2015Year of publication
- Publication date
-
2015-01-01Full publication date if available
- Authors
-
Gordana Timotijević, Mira Milisavljević, Dragana Nikolić, Boško Milovanović, Dragana Nikolić, Miroslav Nikolić, Jelena SamardžićList of authors in order
- Landing page
-
https://doi.org/10.2298/gensr1502405tPublisher landing page
- PDF URL
-
https://www.doiserbia.nb.rs/ft.aspx?id=0534-00121502405TDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
diamondOpen access status per OpenAlex
- OA URL
-
https://www.doiserbia.nb.rs/ft.aspx?id=0534-00121502405TDirect OA link when available
- Concepts
-
microRNA, Biology, Economic shortage, Computational biology, Gene expression, Gene, Gene expression profiling, Stem-loop, Transcription factor, Real-time polymerase chain reaction, Cell biology, Genetics, RNA, Philosophy, Government (linguistics), LinguisticsTop concepts (fields/topics) attached by OpenAlex
- Cited by
-
0Total citation count in OpenAlex
- References (count)
-
17Number of works referenced by this work
- Related works (count)
-
10Other works algorithmically related by OpenAlex
Full payload
| id | https://openalex.org/W2301058572 |
|---|---|
| doi | https://doi.org/10.2298/gensr1502405t |
| ids.doi | https://doi.org/10.2298/gensr1502405t |
| ids.mag | 2301058572 |
| ids.openalex | https://openalex.org/W2301058572 |
| fwci | 0.0 |
| type | article |
| title | Establishment and in-house validation of stem-loop RT PCR method for MicroRNA398 expression analysis |
| awards[0].id | https://openalex.org/G6102986025 |
| awards[0].funder_id | https://openalex.org/F4320322729 |
| awards[0].display_name | |
| awards[0].funder_award_id | 173005 i ON-173028 |
| awards[0].funder_display_name | Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja |
| biblio.issue | 2 |
| biblio.volume | 47 |
| biblio.last_page | 416 |
| biblio.first_page | 405 |
| topics[0].id | https://openalex.org/T10434 |
| topics[0].field.id | https://openalex.org/fields/11 |
| topics[0].field.display_name | Agricultural and Biological Sciences |
| topics[0].score | 0.9944999814033508 |
| topics[0].domain.id | https://openalex.org/domains/1 |
| topics[0].domain.display_name | Life Sciences |
| topics[0].subfield.id | https://openalex.org/subfields/1110 |
| topics[0].subfield.display_name | Plant Science |
| topics[0].display_name | Chromosomal and Genetic Variations |
| topics[1].id | https://openalex.org/T10184 |
| topics[1].field.id | https://openalex.org/fields/11 |
| topics[1].field.display_name | Agricultural and Biological Sciences |
| topics[1].score | 0.991599977016449 |
| topics[1].domain.id | https://openalex.org/domains/1 |
| topics[1].domain.display_name | Life Sciences |
| topics[1].subfield.id | https://openalex.org/subfields/1110 |
| topics[1].subfield.display_name | Plant Science |
| topics[1].display_name | Plant Molecular Biology Research |
| topics[2].id | https://openalex.org/T10062 |
| topics[2].field.id | https://openalex.org/fields/13 |
| topics[2].field.display_name | Biochemistry, Genetics and Molecular Biology |
| topics[2].score | 0.9904999732971191 |
| topics[2].domain.id | https://openalex.org/domains/1 |
| topics[2].domain.display_name | Life Sciences |
| topics[2].subfield.id | https://openalex.org/subfields/1306 |
| topics[2].subfield.display_name | Cancer Research |
| topics[2].display_name | MicroRNA in disease regulation |
| funders[0].id | https://openalex.org/F4320322729 |
| funders[0].ror | https://ror.org/01znas443 |
| funders[0].display_name | Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja |
| is_xpac | False |
| apc_list | |
| apc_paid | |
| concepts[0].id | https://openalex.org/C145059251 |
| concepts[0].level | 3 |
| concepts[0].score | 0.8050802946090698 |
| concepts[0].wikidata | https://www.wikidata.org/wiki/Q310899 |
| concepts[0].display_name | microRNA |
| concepts[1].id | https://openalex.org/C86803240 |
| concepts[1].level | 0 |
| concepts[1].score | 0.6428568959236145 |
| concepts[1].wikidata | https://www.wikidata.org/wiki/Q420 |
| concepts[1].display_name | Biology |
| concepts[2].id | https://openalex.org/C194051981 |
| concepts[2].level | 3 |
| concepts[2].score | 0.6087362766265869 |
| concepts[2].wikidata | https://www.wikidata.org/wiki/Q1337691 |
| concepts[2].display_name | Economic shortage |
| concepts[3].id | https://openalex.org/C70721500 |
| concepts[3].level | 1 |
| concepts[3].score | 0.5900038480758667 |
| concepts[3].wikidata | https://www.wikidata.org/wiki/Q177005 |
| concepts[3].display_name | Computational biology |
| concepts[4].id | https://openalex.org/C150194340 |
| concepts[4].level | 3 |
| concepts[4].score | 0.5243092775344849 |
| concepts[4].wikidata | https://www.wikidata.org/wiki/Q26972 |
| concepts[4].display_name | Gene expression |
| concepts[5].id | https://openalex.org/C104317684 |
| concepts[5].level | 2 |
| concepts[5].score | 0.4807721972465515 |
| concepts[5].wikidata | https://www.wikidata.org/wiki/Q7187 |
| concepts[5].display_name | Gene |
| concepts[6].id | https://openalex.org/C18431079 |
| concepts[6].level | 4 |
| concepts[6].score | 0.4461801052093506 |
| concepts[6].wikidata | https://www.wikidata.org/wiki/Q1502169 |
| concepts[6].display_name | Gene expression profiling |
| concepts[7].id | https://openalex.org/C169663058 |
| concepts[7].level | 4 |
| concepts[7].score | 0.42706534266471863 |
| concepts[7].wikidata | https://www.wikidata.org/wiki/Q418900 |
| concepts[7].display_name | Stem-loop |
| concepts[8].id | https://openalex.org/C86339819 |
| concepts[8].level | 3 |
| concepts[8].score | 0.42457297444343567 |
| concepts[8].wikidata | https://www.wikidata.org/wiki/Q407384 |
| concepts[8].display_name | Transcription factor |
| concepts[9].id | https://openalex.org/C48023723 |
| concepts[9].level | 3 |
| concepts[9].score | 0.4173964858055115 |
| concepts[9].wikidata | https://www.wikidata.org/wiki/Q856198 |
| concepts[9].display_name | Real-time polymerase chain reaction |
| concepts[10].id | https://openalex.org/C95444343 |
| concepts[10].level | 1 |
| concepts[10].score | 0.32005250453948975 |
| concepts[10].wikidata | https://www.wikidata.org/wiki/Q7141 |
| concepts[10].display_name | Cell biology |
| concepts[11].id | https://openalex.org/C54355233 |
| concepts[11].level | 1 |
| concepts[11].score | 0.27101558446884155 |
| concepts[11].wikidata | https://www.wikidata.org/wiki/Q7162 |
| concepts[11].display_name | Genetics |
| concepts[12].id | https://openalex.org/C67705224 |
| concepts[12].level | 3 |
| concepts[12].score | 0.25601285696029663 |
| concepts[12].wikidata | https://www.wikidata.org/wiki/Q11053 |
| concepts[12].display_name | RNA |
| concepts[13].id | https://openalex.org/C138885662 |
| concepts[13].level | 0 |
| concepts[13].score | 0.0 |
| concepts[13].wikidata | https://www.wikidata.org/wiki/Q5891 |
| concepts[13].display_name | Philosophy |
| concepts[14].id | https://openalex.org/C2778137410 |
| concepts[14].level | 2 |
| concepts[14].score | 0.0 |
| concepts[14].wikidata | https://www.wikidata.org/wiki/Q2732820 |
| concepts[14].display_name | Government (linguistics) |
| concepts[15].id | https://openalex.org/C41895202 |
| concepts[15].level | 1 |
| concepts[15].score | 0.0 |
| concepts[15].wikidata | https://www.wikidata.org/wiki/Q8162 |
| concepts[15].display_name | Linguistics |
| keywords[0].id | https://openalex.org/keywords/microrna |
| keywords[0].score | 0.8050802946090698 |
| keywords[0].display_name | microRNA |
| keywords[1].id | https://openalex.org/keywords/biology |
| keywords[1].score | 0.6428568959236145 |
| keywords[1].display_name | Biology |
| keywords[2].id | https://openalex.org/keywords/economic-shortage |
| keywords[2].score | 0.6087362766265869 |
| keywords[2].display_name | Economic shortage |
| keywords[3].id | https://openalex.org/keywords/computational-biology |
| keywords[3].score | 0.5900038480758667 |
| keywords[3].display_name | Computational biology |
| keywords[4].id | https://openalex.org/keywords/gene-expression |
| keywords[4].score | 0.5243092775344849 |
| keywords[4].display_name | Gene expression |
| keywords[5].id | https://openalex.org/keywords/gene |
| keywords[5].score | 0.4807721972465515 |
| keywords[5].display_name | Gene |
| keywords[6].id | https://openalex.org/keywords/gene-expression-profiling |
| keywords[6].score | 0.4461801052093506 |
| keywords[6].display_name | Gene expression profiling |
| keywords[7].id | https://openalex.org/keywords/stem-loop |
| keywords[7].score | 0.42706534266471863 |
| keywords[7].display_name | Stem-loop |
| keywords[8].id | https://openalex.org/keywords/transcription-factor |
| keywords[8].score | 0.42457297444343567 |
| keywords[8].display_name | Transcription factor |
| keywords[9].id | https://openalex.org/keywords/real-time-polymerase-chain-reaction |
| keywords[9].score | 0.4173964858055115 |
| keywords[9].display_name | Real-time polymerase chain reaction |
| keywords[10].id | https://openalex.org/keywords/cell-biology |
| keywords[10].score | 0.32005250453948975 |
| keywords[10].display_name | Cell biology |
| keywords[11].id | https://openalex.org/keywords/genetics |
| keywords[11].score | 0.27101558446884155 |
| keywords[11].display_name | Genetics |
| keywords[12].id | https://openalex.org/keywords/rna |
| keywords[12].score | 0.25601285696029663 |
| keywords[12].display_name | RNA |
| language | en |
| locations[0].id | doi:10.2298/gensr1502405t |
| locations[0].is_oa | True |
| locations[0].source.id | https://openalex.org/S4210171837 |
| locations[0].source.issn | 0534-0012, 1820-6069 |
| locations[0].source.type | journal |
| locations[0].source.is_oa | True |
| locations[0].source.issn_l | 0534-0012 |
| locations[0].source.is_core | True |
| locations[0].source.is_in_doaj | False |
| locations[0].source.display_name | Genetika |
| locations[0].source.host_organization | https://openalex.org/P4310321594 |
| locations[0].source.host_organization_name | Serbian Genetics Society |
| locations[0].source.host_organization_lineage | https://openalex.org/P4310321594 |
| locations[0].source.host_organization_lineage_names | Serbian Genetics Society |
| locations[0].license | |
| locations[0].pdf_url | http://www.doiserbia.nb.rs/ft.aspx?id=0534-00121502405T |
| locations[0].version | publishedVersion |
| locations[0].raw_type | journal-article |
| locations[0].license_id | |
| locations[0].is_accepted | True |
| locations[0].is_published | True |
| locations[0].raw_source_name | Genetika |
| locations[0].landing_page_url | https://doi.org/10.2298/gensr1502405t |
| locations[1].id | pmh:oai:doaj.org/article:0d2cea68c1c343a1b8579578a1ef82c9 |
| locations[1].is_oa | True |
| locations[1].source.id | https://openalex.org/S4306401280 |
| locations[1].source.issn | |
| locations[1].source.type | repository |
| locations[1].source.is_oa | False |
| locations[1].source.issn_l | |
| locations[1].source.is_core | False |
| locations[1].source.is_in_doaj | False |
| locations[1].source.display_name | DOAJ (DOAJ: Directory of Open Access Journals) |
| locations[1].source.host_organization | |
| locations[1].source.host_organization_name | |
| locations[1].license | cc-by-sa |
| locations[1].pdf_url | |
| locations[1].version | submittedVersion |
| locations[1].raw_type | article |
| locations[1].license_id | https://openalex.org/licenses/cc-by-sa |
| locations[1].is_accepted | False |
| locations[1].is_published | False |
| locations[1].raw_source_name | Genetika, Vol 47, Iss 2, Pp 405-416 (2015) |
| locations[1].landing_page_url | https://doaj.org/article/0d2cea68c1c343a1b8579578a1ef82c9 |
| locations[2].id | pmh:oai:doiserbia:0534-00121502405T |
| locations[2].is_oa | False |
| locations[2].source.id | https://openalex.org/S4306400515 |
| locations[2].source.issn | |
| locations[2].source.type | repository |
| locations[2].source.is_oa | False |
| locations[2].source.issn_l | |
| locations[2].source.is_core | False |
| locations[2].source.is_in_doaj | False |
| locations[2].source.display_name | Digital Object Identifier (DOI) Repository Serbia (National Library of Serbia) |
| locations[2].source.host_organization | https://openalex.org/I4210142455 |
| locations[2].source.host_organization_name | National Library of Serbia |
| locations[2].source.host_organization_lineage | https://openalex.org/I4210142455 |
| locations[2].license | |
| locations[2].pdf_url | |
| locations[2].version | submittedVersion |
| locations[2].raw_type | . 47(2) 405-416 |
| locations[2].license_id | |
| locations[2].is_accepted | False |
| locations[2].is_published | False |
| locations[2].raw_source_name | |
| locations[2].landing_page_url | http://doi.org/10.2298/GENSR1502405T |
| locations[3].id | pmh:oai:imagine.imgge.bg.ac.rs:123456789/843 |
| locations[3].is_oa | True |
| locations[3].source.id | https://openalex.org/S4377196579 |
| locations[3].source.issn | |
| locations[3].source.type | repository |
| locations[3].source.is_oa | False |
| locations[3].source.issn_l | |
| locations[3].source.is_core | False |
| locations[3].source.is_in_doaj | False |
| locations[3].source.display_name | IMAGINE - Repository of the Institute of molecular genetics and genetic engineering (University of Belgrade) |
| locations[3].source.host_organization | https://openalex.org/I4068193 |
| locations[3].source.host_organization_name | University of Belgrade |
| locations[3].source.host_organization_lineage | https://openalex.org/I4068193 |
| locations[3].license | cc-by-nc-nd |
| locations[3].pdf_url | |
| locations[3].version | submittedVersion |
| locations[3].raw_type | article |
| locations[3].license_id | https://openalex.org/licenses/cc-by-nc-nd |
| locations[3].is_accepted | False |
| locations[3].is_published | False |
| locations[3].raw_source_name | Genetika-Belgrade |
| locations[3].landing_page_url | https://imagine.imgge.bg.ac.rs/handle/123456789/843 |
| locations[4].id | pmh:oai:rimsi.imsi.bg.ac.rs:123456789/848 |
| locations[4].is_oa | True |
| locations[4].source.id | https://openalex.org/S4306402458 |
| locations[4].source.issn | |
| locations[4].source.type | repository |
| locations[4].source.is_oa | False |
| locations[4].source.issn_l | |
| locations[4].source.is_core | False |
| locations[4].source.is_in_doaj | False |
| locations[4].source.display_name | RIMSI (Institute for Multidisciplinary Research, University of Belgrade) |
| locations[4].source.host_organization | https://openalex.org/I4210101313 |
| locations[4].source.host_organization_name | University Hospital Center Dr Dragiša Mišović |
| locations[4].source.host_organization_lineage | https://openalex.org/I4210101313 |
| locations[4].license | cc-by-nc-nd |
| locations[4].pdf_url | |
| locations[4].version | submittedVersion |
| locations[4].raw_type | article |
| locations[4].license_id | https://openalex.org/licenses/cc-by-nc-nd |
| locations[4].is_accepted | False |
| locations[4].is_published | False |
| locations[4].raw_source_name | Genetika-Belgrade |
| locations[4].landing_page_url | http://rimsi.imsi.bg.ac.rs/handle/123456789/848 |
| indexed_in | crossref, doaj |
| authorships[0].author.id | https://openalex.org/A5029094269 |
| authorships[0].author.orcid | https://orcid.org/0000-0002-5716-9419 |
| authorships[0].author.display_name | Gordana Timotijević |
| authorships[0].countries | RS |
| authorships[0].affiliations[0].institution_ids | https://openalex.org/I4068193 |
| authorships[0].affiliations[0].raw_affiliation_string | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| authorships[0].institutions[0].id | https://openalex.org/I4068193 |
| authorships[0].institutions[0].ror | https://ror.org/02qsmb048 |
| authorships[0].institutions[0].type | education |
| authorships[0].institutions[0].lineage | https://openalex.org/I4068193 |
| authorships[0].institutions[0].country_code | RS |
| authorships[0].institutions[0].display_name | University of Belgrade |
| authorships[0].author_position | first |
| authorships[0].raw_author_name | Gordana Timotijevic |
| authorships[0].is_corresponding | True |
| authorships[0].raw_affiliation_strings | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| authorships[1].author.id | https://openalex.org/A5035103744 |
| authorships[1].author.orcid | https://orcid.org/0000-0002-0382-1360 |
| authorships[1].author.display_name | Mira Milisavljević |
| authorships[1].countries | RS |
| authorships[1].affiliations[0].institution_ids | https://openalex.org/I4068193 |
| authorships[1].affiliations[0].raw_affiliation_string | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| authorships[1].institutions[0].id | https://openalex.org/I4068193 |
| authorships[1].institutions[0].ror | https://ror.org/02qsmb048 |
| authorships[1].institutions[0].type | education |
| authorships[1].institutions[0].lineage | https://openalex.org/I4068193 |
| authorships[1].institutions[0].country_code | RS |
| authorships[1].institutions[0].display_name | University of Belgrade |
| authorships[1].author_position | middle |
| authorships[1].raw_author_name | Mira Milisavljevic |
| authorships[1].is_corresponding | False |
| authorships[1].raw_affiliation_strings | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| authorships[2].author.id | https://openalex.org/A5029589655 |
| authorships[2].author.orcid | https://orcid.org/0000-0002-9812-062X |
| authorships[2].author.display_name | Dragana Nikolić |
| authorships[2].countries | RS |
| authorships[2].affiliations[0].institution_ids | https://openalex.org/I4068193 |
| authorships[2].affiliations[0].raw_affiliation_string | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| authorships[2].institutions[0].id | https://openalex.org/I4068193 |
| authorships[2].institutions[0].ror | https://ror.org/02qsmb048 |
| authorships[2].institutions[0].type | education |
| authorships[2].institutions[0].lineage | https://openalex.org/I4068193 |
| authorships[2].institutions[0].country_code | RS |
| authorships[2].institutions[0].display_name | University of Belgrade |
| authorships[2].author_position | middle |
| authorships[2].raw_author_name | Dragana Nikolic |
| authorships[2].is_corresponding | False |
| authorships[2].raw_affiliation_strings | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| authorships[3].author.id | https://openalex.org/A5051845795 |
| authorships[3].author.orcid | https://orcid.org/0000-0001-7080-7334 |
| authorships[3].author.display_name | Boško Milovanović |
| authorships[3].countries | HU, RS |
| authorships[3].affiliations[0].institution_ids | https://openalex.org/I1338511612, https://openalex.org/I4210122271 |
| authorships[3].affiliations[0].raw_affiliation_string | Serbian Academy of Sciences and Arts, Geographical Institute "Jovan Cvijić", Belgrade |
| authorships[3].institutions[0].id | https://openalex.org/I4210122271 |
| authorships[3].institutions[0].ror | https://ror.org/02j8vpa07 |
| authorships[3].institutions[0].type | facility |
| authorships[3].institutions[0].lineage | https://openalex.org/I4210122271, https://openalex.org/I4210128682, https://openalex.org/I7597260 |
| authorships[3].institutions[0].country_code | HU |
| authorships[3].institutions[0].display_name | Geographical Institute |
| authorships[3].institutions[1].id | https://openalex.org/I1338511612 |
| authorships[3].institutions[1].ror | https://ror.org/05m1y4204 |
| authorships[3].institutions[1].type | government |
| authorships[3].institutions[1].lineage | https://openalex.org/I1338511612 |
| authorships[3].institutions[1].country_code | RS |
| authorships[3].institutions[1].display_name | Serbian Academy of Sciences and Arts |
| authorships[3].author_position | middle |
| authorships[3].raw_author_name | Bosko Milovanovic |
| authorships[3].is_corresponding | False |
| authorships[3].raw_affiliation_strings | Serbian Academy of Sciences and Arts, Geographical Institute "Jovan Cvijić", Belgrade |
| authorships[4].author.id | https://openalex.org/A5029589655 |
| authorships[4].author.orcid | https://orcid.org/0000-0002-9812-062X |
| authorships[4].author.display_name | Dragana Nikolić |
| authorships[4].countries | RS |
| authorships[4].affiliations[0].institution_ids | https://openalex.org/I4068193 |
| authorships[4].affiliations[0].raw_affiliation_string | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| authorships[4].institutions[0].id | https://openalex.org/I4068193 |
| authorships[4].institutions[0].ror | https://ror.org/02qsmb048 |
| authorships[4].institutions[0].type | education |
| authorships[4].institutions[0].lineage | https://openalex.org/I4068193 |
| authorships[4].institutions[0].country_code | RS |
| authorships[4].institutions[0].display_name | University of Belgrade |
| authorships[4].author_position | middle |
| authorships[4].raw_author_name | Dragana Nikolic |
| authorships[4].is_corresponding | False |
| authorships[4].raw_affiliation_strings | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| authorships[5].author.id | https://openalex.org/A5086771408 |
| authorships[5].author.orcid | https://orcid.org/0000-0001-9643-138X |
| authorships[5].author.display_name | Miroslav Nikolić |
| authorships[5].countries | RS |
| authorships[5].affiliations[0].institution_ids | https://openalex.org/I4068193 |
| authorships[5].affiliations[0].raw_affiliation_string | Plant Nutrition Research Group, Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia |
| authorships[5].institutions[0].id | https://openalex.org/I4068193 |
| authorships[5].institutions[0].ror | https://ror.org/02qsmb048 |
| authorships[5].institutions[0].type | education |
| authorships[5].institutions[0].lineage | https://openalex.org/I4068193 |
| authorships[5].institutions[0].country_code | RS |
| authorships[5].institutions[0].display_name | University of Belgrade |
| authorships[5].author_position | middle |
| authorships[5].raw_author_name | Miroslav Nikolic |
| authorships[5].is_corresponding | False |
| authorships[5].raw_affiliation_strings | Plant Nutrition Research Group, Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia |
| authorships[6].author.id | https://openalex.org/A5076732289 |
| authorships[6].author.orcid | https://orcid.org/0000-0002-9923-563X |
| authorships[6].author.display_name | Jelena Samardžić |
| authorships[6].countries | RS |
| authorships[6].affiliations[0].institution_ids | https://openalex.org/I4068193 |
| authorships[6].affiliations[0].raw_affiliation_string | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| authorships[6].institutions[0].id | https://openalex.org/I4068193 |
| authorships[6].institutions[0].ror | https://ror.org/02qsmb048 |
| authorships[6].institutions[0].type | education |
| authorships[6].institutions[0].lineage | https://openalex.org/I4068193 |
| authorships[6].institutions[0].country_code | RS |
| authorships[6].institutions[0].display_name | University of Belgrade |
| authorships[6].author_position | last |
| authorships[6].raw_author_name | Jelena Samardzic |
| authorships[6].is_corresponding | False |
| authorships[6].raw_affiliation_strings | Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia, |
| has_content.pdf | True |
| has_content.grobid_xml | True |
| is_paratext | False |
| open_access.is_oa | True |
| open_access.oa_url | http://www.doiserbia.nb.rs/ft.aspx?id=0534-00121502405T |
| open_access.oa_status | diamond |
| open_access.any_repository_has_fulltext | False |
| created_date | 2025-10-10T00:00:00 |
| display_name | Establishment and in-house validation of stem-loop RT PCR method for MicroRNA398 expression analysis |
| has_fulltext | True |
| is_retracted | False |
| updated_date | 2025-11-06T03:46:38.306776 |
| primary_topic.id | https://openalex.org/T10434 |
| primary_topic.field.id | https://openalex.org/fields/11 |
| primary_topic.field.display_name | Agricultural and Biological Sciences |
| primary_topic.score | 0.9944999814033508 |
| primary_topic.domain.id | https://openalex.org/domains/1 |
| primary_topic.domain.display_name | Life Sciences |
| primary_topic.subfield.id | https://openalex.org/subfields/1110 |
| primary_topic.subfield.display_name | Plant Science |
| primary_topic.display_name | Chromosomal and Genetic Variations |
| related_works | https://openalex.org/W2579473328, https://openalex.org/W2509147714, https://openalex.org/W4239898203, https://openalex.org/W2586833044, https://openalex.org/W2603666417, https://openalex.org/W46955353, https://openalex.org/W2315021981, https://openalex.org/W2955295882, https://openalex.org/W2145869671, https://openalex.org/W2737319532 |
| cited_by_count | 0 |
| locations_count | 5 |
| best_oa_location.id | doi:10.2298/gensr1502405t |
| best_oa_location.is_oa | True |
| best_oa_location.source.id | https://openalex.org/S4210171837 |
| best_oa_location.source.issn | 0534-0012, 1820-6069 |
| best_oa_location.source.type | journal |
| best_oa_location.source.is_oa | True |
| best_oa_location.source.issn_l | 0534-0012 |
| best_oa_location.source.is_core | True |
| best_oa_location.source.is_in_doaj | False |
| best_oa_location.source.display_name | Genetika |
| best_oa_location.source.host_organization | https://openalex.org/P4310321594 |
| best_oa_location.source.host_organization_name | Serbian Genetics Society |
| best_oa_location.source.host_organization_lineage | https://openalex.org/P4310321594 |
| best_oa_location.source.host_organization_lineage_names | Serbian Genetics Society |
| best_oa_location.license | |
| best_oa_location.pdf_url | http://www.doiserbia.nb.rs/ft.aspx?id=0534-00121502405T |
| best_oa_location.version | publishedVersion |
| best_oa_location.raw_type | journal-article |
| best_oa_location.license_id | |
| best_oa_location.is_accepted | True |
| best_oa_location.is_published | True |
| best_oa_location.raw_source_name | Genetika |
| best_oa_location.landing_page_url | https://doi.org/10.2298/gensr1502405t |
| primary_location.id | doi:10.2298/gensr1502405t |
| primary_location.is_oa | True |
| primary_location.source.id | https://openalex.org/S4210171837 |
| primary_location.source.issn | 0534-0012, 1820-6069 |
| primary_location.source.type | journal |
| primary_location.source.is_oa | True |
| primary_location.source.issn_l | 0534-0012 |
| primary_location.source.is_core | True |
| primary_location.source.is_in_doaj | False |
| primary_location.source.display_name | Genetika |
| primary_location.source.host_organization | https://openalex.org/P4310321594 |
| primary_location.source.host_organization_name | Serbian Genetics Society |
| primary_location.source.host_organization_lineage | https://openalex.org/P4310321594 |
| primary_location.source.host_organization_lineage_names | Serbian Genetics Society |
| primary_location.license | |
| primary_location.pdf_url | http://www.doiserbia.nb.rs/ft.aspx?id=0534-00121502405T |
| primary_location.version | publishedVersion |
| primary_location.raw_type | journal-article |
| primary_location.license_id | |
| primary_location.is_accepted | True |
| primary_location.is_published | True |
| primary_location.raw_source_name | Genetika |
| primary_location.landing_page_url | https://doi.org/10.2298/gensr1502405t |
| publication_date | 2015-01-01 |
| publication_year | 2015 |
| referenced_works | https://openalex.org/W2057824004, https://openalex.org/W2009540277, https://openalex.org/W2042325491, https://openalex.org/W2529188053, https://openalex.org/W2069870317, https://openalex.org/W2076497558, https://openalex.org/W2015969705, https://openalex.org/W2117391818, https://openalex.org/W2011518477, https://openalex.org/W2001911979, https://openalex.org/W2027503834, https://openalex.org/W2161222316, https://openalex.org/W2056078276, https://openalex.org/W1987438700, https://openalex.org/W1966612206, https://openalex.org/W2071671704, https://openalex.org/W2143804344 |
| referenced_works_count | 17 |
| abstract_inverted_index.a | 121, 181 |
| abstract_inverted_index.In | 116 |
| abstract_inverted_index.RT | 154 |
| abstract_inverted_index.We | 69 |
| abstract_inverted_index.as | 16, 18, 131, 133, 151 |
| abstract_inverted_index.by | 95, 168 |
| abstract_inverted_index.in | 19, 42, 82, 98, 191 |
| abstract_inverted_index.is | 105 |
| abstract_inverted_index.of | 6, 27, 36, 56, 61, 107, 112, 138, 146, 159, 170, 176, 188 |
| abstract_inverted_index.on | 185 |
| abstract_inverted_index.or | 44 |
| abstract_inverted_index.to | 3, 22, 47, 72 |
| abstract_inverted_index.we | 119 |
| abstract_inverted_index.PCR | 103, 141 |
| abstract_inverted_index.The | 164 |
| abstract_inverted_index.all | 189 |
| abstract_inverted_index.and | 34, 136, 161 |
| abstract_inverted_index.are | 30, 41 |
| abstract_inverted_index.for | 32, 50, 64, 77, 110, 123, 129 |
| abstract_inverted_index.kit | 183 |
| abstract_inverted_index.one | 106 |
| abstract_inverted_index.out | 71 |
| abstract_inverted_index.set | 70 |
| abstract_inverted_index.the | 4, 75, 144, 171 |
| abstract_inverted_index.was | 149, 166 |
| abstract_inverted_index.RNAs | 9, 190 |
| abstract_inverted_index.Real | 101, 139 |
| abstract_inverted_index.Some | 26 |
| abstract_inverted_index.data | 173 |
| abstract_inverted_index.gene | 67, 79, 114 |
| abstract_inverted_index.have | 11 |
| abstract_inverted_index.iron | 91 |
| abstract_inverted_index.this | 117, 147 |
| abstract_inverted_index.time | 102, 140 |
| abstract_inverted_index.well | 17, 132 |
| abstract_inverted_index.when | 39 |
| abstract_inverted_index.with | 100, 174 |
| abstract_inverted_index.?stem | 124, 152 |
| abstract_inverted_index.based | 184 |
| abstract_inverted_index.class | 5 |
| abstract_inverted_index.grown | 84 |
| abstract_inverted_index.loop? | 125, 153 |
| abstract_inverted_index.miRNA | 78, 113 |
| abstract_inverted_index.paper | 118 |
| abstract_inverted_index.plant | 20, 28, 52 |
| abstract_inverted_index.roles | 13 |
| abstract_inverted_index.small | 7 |
| abstract_inverted_index.their | 66 |
| abstract_inverted_index.under | 85 |
| abstract_inverted_index.using | 180 |
| abstract_inverted_index.which | 10, 155 |
| abstract_inverted_index.Better | 54 |
| abstract_inverted_index.belong | 2 |
| abstract_inverted_index.cDNAs. | 192 |
| abstract_inverted_index.design | 127 |
| abstract_inverted_index.excess | 43 |
| abstract_inverted_index.method | 104, 122, 148, 165 |
| abstract_inverted_index.miR398 | 177 |
| abstract_inverted_index.miRNAs | 29, 57 |
| abstract_inverted_index.primer | 126 |
| abstract_inverted_index.target | 160 |
| abstract_inverted_index.Proving | 143 |
| abstract_inverted_index.Reverse | 93 |
| abstract_inverted_index.certain | 51 |
| abstract_inverted_index.diverse | 23 |
| abstract_inverted_index.implies | 59 |
| abstract_inverted_index.miR398, | 130 |
| abstract_inverted_index.mineral | 87 |
| abstract_inverted_index.optimal | 48 |
| abstract_inverted_index.primers | 97 |
| abstract_inverted_index.results | 175 |
| abstract_inverted_index.(miRNAs) | 1 |
| abstract_inverted_index.accuracy | 145 |
| abstract_inverted_index.achieved | 179 |
| abstract_inverted_index.analysis | 81 |
| abstract_inverted_index.consider | 156 |
| abstract_inverted_index.control. | 163 |
| abstract_inverted_index.cucumber | 83 |
| abstract_inverted_index.nutrient | 88 |
| abstract_inverted_index.obtained | 172 |
| abstract_inverted_index.reaction | 134 |
| abstract_inverted_index.regimes, | 89 |
| abstract_inverted_index.response | 21 |
| abstract_inverted_index.separate | 157 |
| abstract_inverted_index.shortage | 45 |
| abstract_inverted_index.species. | 53 |
| abstract_inverted_index.specific | 128 |
| abstract_inverted_index.validate | 74 |
| abstract_inverted_index.verified | 167 |
| abstract_inverted_index.MicroRNAs | 0 |
| abstract_inverted_index.comparing | 46 |
| abstract_inverted_index.efficient | 62 |
| abstract_inverted_index.essential | 31 |
| abstract_inverted_index.establish | 73 |
| abstract_inverted_index.functions | 58 |
| abstract_inverted_index.important | 12 |
| abstract_inverted_index.including | 90 |
| abstract_inverted_index.nutrients | 40 |
| abstract_inverted_index.nutritive | 37 |
| abstract_inverted_index.potential | 108 |
| abstract_inverted_index.presented | 120 |
| abstract_inverted_index.profiling | 65 |
| abstract_inverted_index.stresses. | 25 |
| abstract_inverted_index.approaches | 109 |
| abstract_inverted_index.commercial | 182 |
| abstract_inverted_index.comparison | 169 |
| abstract_inverted_index.conversion | 187 |
| abstract_inverted_index.endogenous | 162 |
| abstract_inverted_index.expression | 80, 178 |
| abstract_inverted_index.imperative | 150 |
| abstract_inverted_index.non-coding | 8 |
| abstract_inverted_index.regulation | 33 |
| abstract_inverted_index.suboptimal | 86 |
| abstract_inverted_index.technology | 63 |
| abstract_inverted_index.throughout | 14 |
| abstract_inverted_index.?stem-loop? | 96 |
| abstract_inverted_index.combination | 99 |
| abstract_inverted_index.deficiency. | 92 |
| abstract_inverted_index.development | 15, 60 |
| abstract_inverted_index.efficiency. | 142 |
| abstract_inverted_index.expression. | 68, 115 |
| abstract_inverted_index.homeostasis | 38 |
| abstract_inverted_index.maintenance | 35 |
| abstract_inverted_index.methodology | 76 |
| abstract_inverted_index.optimization | 135 |
| abstract_inverted_index.simultaneous | 186 |
| abstract_inverted_index.concentration | 49 |
| abstract_inverted_index.determination | 137 |
| abstract_inverted_index.environmental | 24 |
| abstract_inverted_index.transcription | 94, 158 |
| abstract_inverted_index.understanding | 55 |
| abstract_inverted_index.quantification | 111 |
| cited_by_percentile_year | |
| corresponding_author_ids | https://openalex.org/A5029094269 |
| countries_distinct_count | 2 |
| institutions_distinct_count | 7 |
| corresponding_institution_ids | https://openalex.org/I4068193 |
| citation_normalized_percentile.value | 0.08754982 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |