Establishment of a high-throughput scale-down clone screening platform for intensified fed-batch culture of CHO cells Article Swipe
Haiyan Luo
,
Shuai Wang
,
Collin Chong
,
Liang Wang
,
Xiaojun Sun
,
Qian Guo
,
Sam Zhang
,
Xiaoyue Chen
,
Hang Zhou
,
Weichang Zhou
·
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-5122178/v1
YOU?
·
· 2024
· Open Access
·
· DOI: https://doi.org/10.21203/rs.3.rs-5122178/v1
Purpose To develop a scale-down clone screening platform for the intensified fed-batch (IFB) process to allow efficient identification of high expressing clones fitting the IFB culture strategy in bioreactor. Results Three monoclonal antibodies (mAbs) were used in the development and validation of the IFB specific clone screening platform for CHO cells. The IFB platform significantly improved titer levels, achieving an average titer of 8 g/L and the highest titer of 9.6 g/L. With similar cell viability, lactate profile and titer levels, both the spin tube model and the AMBR250@ bioreactor system were effective in screening clones suitable for IFB process. The addition of aurintricarboxylic acid (ATA) and uridine in the process optimisation led to a further increase in expression levels in both systems, achieving the highest titer of 12.2g/L. Conclusion This IFB-process specific clone screening serves as an alternative platform that can increase the effectiveness and efficiency of screening high-expressing CHO cell lines for IFB production.
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Metadata
- Type
- preprint
- Language
- en
- Landing Page
- https://doi.org/10.21203/rs.3.rs-5122178/v1
- https://www.researchsquare.com/article/rs-5122178/latest.pdf
- OA Status
- gold
- Related Works
- 10
- OpenAlex ID
- https://openalex.org/W4404498872
All OpenAlex metadata
Raw OpenAlex JSON
- OpenAlex ID
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https://openalex.org/W4404498872Canonical identifier for this work in OpenAlex
- DOI
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https://doi.org/10.21203/rs.3.rs-5122178/v1Digital Object Identifier
- Title
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Establishment of a high-throughput scale-down clone screening platform for intensified fed-batch culture of CHO cellsWork title
- Type
-
preprintOpenAlex work type
- Language
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enPrimary language
- Publication year
-
2024Year of publication
- Publication date
-
2024-11-19Full publication date if available
- Authors
-
Haiyan Luo, Shuai Wang, Collin Chong, Liang Wang, Xiaojun Sun, Qian Guo, Sam Zhang, Xiaoyue Chen, Hang Zhou, Weichang ZhouList of authors in order
- Landing page
-
https://doi.org/10.21203/rs.3.rs-5122178/v1Publisher landing page
- PDF URL
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https://www.researchsquare.com/article/rs-5122178/latest.pdfDirect link to full text PDF
- Open access
-
YesWhether a free full text is available
- OA status
-
goldOpen access status per OpenAlex
- OA URL
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https://www.researchsquare.com/article/rs-5122178/latest.pdfDirect OA link when available
- Concepts
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clone (Java method), Chinese hamster ovary cell, Throughput, Scale (ratio), High-throughput screening, Computational biology, Computer science, Biology, Cell culture, Genetics, Operating system, Gene, Geography, Cartography, WirelessTop concepts (fields/topics) attached by OpenAlex
- Cited by
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0Total citation count in OpenAlex
- Related works (count)
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10Other works algorithmically related by OpenAlex
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| countries_distinct_count | 0 |
| institutions_distinct_count | 10 |
| citation_normalized_percentile.value | 0.22813202 |
| citation_normalized_percentile.is_in_top_1_percent | False |
| citation_normalized_percentile.is_in_top_10_percent | False |